TXN (NM_003329) Human Untagged Clone
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | TXN |
| Synonyms | TRDX; TRX; TRX1; Trx80 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_003329 |
| Insert Size | 630 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003329.2, NP_003320.2 |
| RefSeq Size | 508 bp |
| RefSeq ORF | 318 bp |
| Locus ID | 7295 |
| UniProt ID | P10599 |
| Cytogenetics | 9q31.3 |
| Domains | thiored |
| Protein Families | Druggable Genome |
| Summary | The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2011 Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003329" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208876 | TXN (Myc-DDK-tagged)-Human thioredoxin (TXN) | 10 ug |
$225.00
|
|
| RC208876L1 | Lenti ORF clone of Human thioredoxin (TXN), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC208876L2 | Lenti ORF clone of Human thioredoxin (TXN), mGFP tagged | 10 ug |
$525.00
|
|
| RC208876L3 | Lenti ORF clone of Human thioredoxin (TXN), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC208876L4 | Lenti ORF clone of Human thioredoxin (TXN), mGFP tagged | 10 ug |
$525.00
|
|
| RG208876 | TXN (tGFP-tagged) - Human thioredoxin (TXN) | 10 ug |
$489.00
|
|
Citations
Truncated and full-length thioredoxin-1 have opposing activating and inhibitory properties for human complement with relevance to endothelial surfaces
King, Nowakowska, Karsten et al
J. Immunol. (2012) 188 (8), 4103-12 DOI: 10.4049/jimmunol.1101295
King, Nowakowska, Karsten et al
J. Immunol. (2012) 188 (8), 4103-12 DOI: 10.4049/jimmunol.1101295
Cited by 32
AI Summary: OriGene contributed to this study by providing the human Trx1 cDNA, which was used for protein expression experiments to investigate the distinct roles of full-length and truncated thioredoxin-1 in complement activation and inhibition.
Snippet: isomerase (PDI) was from ProSpec. Protein expression. Human Trx1 cDNA was obtained from Origene and cloned into pET16b (Novagen) after introduction of NdeI and BamHI restriction sites by PCR. N-terminus His-tagged
The relationship of thioredoxin-1 and cisplatin resistance: its impact on ROS and oxidative metabolism in lung cancer cells
Wangpaichitr, Sullivan, Theodoropoulos et al
Mol. Cancer Ther. (2012) 11 (3), 604-15 DOI: 10.1158/1535-7163.MCT-11-0599
Wangpaichitr, Sullivan, Theodoropoulos et al
Mol. Cancer Ther. (2012) 11 (3), 604-15 DOI: 10.1158/1535-7163.MCT-11-0599
Cited by 3
AI Summary: OriGene’s pCMV6 expression vector was used to clone and overexpress TRX1-cDNA in lung cancer cells, enabling the study of how altered thioredoxin-1 levels affect cisplatin resistance and oxidative metabolism.
Snippet: INTERFERin transfection reagent (Polyplus) was then used to transfect following short interfering RNAs (siRNA;Dharmacon) siTRXC1 (GAAAAGUAUUCCAACGUGA ... TRX1-cDNA with restriction sites SgfI and Mlul was cloned in to the pCMV6 expression vector (Origene).
Showing 1-2 of 2 papers.
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