Semaphorin 7a (SEMA7A) (NM_003612) Human Untagged Clone

SKU SC117884

SEMA7A (untagged)-Human semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Semaphorin 7a
• Synonyms: CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; SEMAK1; SEMAL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117884 sequence for NM_003612 edited (data generated by NextGen Sequencing); ATGACGCCTCCTCCGCCCGGACGTGCCGCCCCCAGCGCACCGCGCGCCCGCGTCCCTGGC; CCGCCGGCTCGGTTGGGGCTTCCGCTGCGGCTGCGGCTGCTGCTGCTGCTCTGGGCGGCC; GCCGCCTCCGCCCAGGGCCACCTAAGGAGCGGACCCCGCATCTTCGCCGTCTGGAAAGGC; CATGTAGGGCAGGACCGGGTGGACTTTGGCCAGACTGAGCCGCACACGGTGCTTTTCCAC; GAGCCAGGCAGCTCCTCTGTGTGGGTGGGAGGACGTGGCAAGGTCTACCTCTTTGACTTC; CCCGAGGGCAAGAACGCATCTGTGCGCACGGTGAATATCGGCTCCACAAAGGGGTCCTGT; CTGGATAAGCGGGACTGCGAGAACTACATCACTCTCCTGGAGAGGCGGAGTGAGGGGCTG; CTGGCCTGTGGCACCAACGCCCGGCACCCCAGCTGCTGGAACCTGGTGAATGGCACTGTG; GTGCCACTTGGCGAGATGAGAGGCTACGCCCCCTTCAGCCCGGACGAGAACTCCCTGGTT; CTGTTTGAAGGGGACGAGGTGTATTCCACCATCCGGAAGCAGGAATACAATGGGAAGATC; CCTCGGTTCCGCCGCATCCGGGGCGAGAGTGAGCTGTACACCAGTGATACTGTCATGCAG; AACCCACAGTTCATCAAAGCCACCATCGTGCACCAAGACCAGGCTTACGATGACAAGATC; TACTACTTCTTCCGAGAGGACAATCCTGACAAGAATCCTGAGGCTCCTCTCAATGTGTCC; CGTGTGGCCCAGTTGTGCAGGGGGGACCAGGGTGGGGAAAGTTCACTGTCAGTCTCCAAG; TGGAACACTTTTCTGAAAGCCATGCTGGTATGCAGTGATGCTGCCACCAACAAGAACTTC; AACAGGCTGCAAGACGTCTTCCTGCTCCCTGACCCCAGCGGCCAGTGGAGGGACACCAGG; GTCTATGGTGTTTTCTCCAACCCCTGGAACTACTCAGCCGTCTGTGTGTATTCCCTCGGT; GACATTGACAAGGTCTTCCGTACCTCCTCACTCAAGGGCTACCACTCAAGCCTTCCCAAC; CCGCGGCCTGGCAAGTGCCTCCCAGACCAGCAGCCGATACCCACAGAGACCTTCCAGGTG; GCTGACCGTCACCCAGAGGTGGCGCAGAGGGTGGAGCCCATGGGGCCTCTGAAGACGCCA; TTGTTCCACTCTAAATACCACTACCAGAAAGTGGCCGTCCACCGCATGCAAGCCAGCCAC; GGGGAGACCTTTCATGTGCTTTACCTAACTACAGACAGGGGCACTATCCACAAGGTGGTG; GAACCGGGGGAGCAGGAGCACAGCTTCGCCTTCAACATCATGGAGATCCAGCCCTTCCGC; CGCGCGGCTGCCATCCAGACCATGTCGCTGGATGCTGAGCGGAGGAAGCTGTATGTGAGC; TCCCAGTGGGAGGTGAGCCAGGTGCCCCTGGACCTGTGTGAGGTCTATGGCGGGGGCTGC; CACGGTTGCCTCATGTCCCGAGACCCCTACTGCGGCTGGGACCAAGGCCGCTGCATCTCC; ATCTACAGCTCCGAACGGTCAGTGCTGCAATCCATTAATCCAGCCGAGCCACACAAGGAG; TGTCCCAACCCCAAACCAGACAAGGCCCCACTGCAGAAGGTTTCCCTGGCCCCAAACTCT; CGCTACTACCTGAGCTGCCCCATGGAATCCCGCCACGCCACCTACTCATGGCGCCACAAG; GAGAACGTGGAGCAGAGCTGCGAACCTGGTCACCAGAGCCCCAACTGCATCCTGTTCATC; GAGAACCTCACGGCGCAGCAGTACGGCCACTACTTCTGCGAGGCCCAGGAGGGCTCCTAC; TTCCGCGAGGCTCAGCACTGGCAGCTGCTGCCCGAGGACGGCATCATGGCCGAGCACCTG; CTGGGTCATGCCTGTGCCCTGGCCGCCTCCCTCTGGCTGGGGGTGCTGCCCACACTCACT; CTTGGCTTGCTGGTCCACTAG; ; Clone variation with respect to NM_003612.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003612 unedited; CCCCCCCCCGNNNNNNNNNNNCNNNCNCNNNNCCCCNCCCCCCCCNNNGGGTTCATAATT; GNATACAATCATATAGGCGGCCGCGAAATTCGCACGAGTCGCTCCCGGGGCCACGGGNAG; ACGCCTCCTCCGCCCGGACGTGCCGCCCCCAGCGCACCGCGCGCCCGCGTCCCTGGCCCG; CCGGCTCGGTTGGGGCTTCCGCTGCGGCTGCGGCTGCTGCTGCTGCTCTGGGCGGCCGCC; GCCTCCGCCCAGGGCCACCTAAGGAGCGGACCCCGCATCTTCGCCGTCTGGAAAGGCCAT; GTAGGGCAGGACCGGGTGGACTTTGGCCAGACTGAGCCGCACACGGTGCTTTTCCACGAG; CCAGGCAGCTCCTCTGTGTGGGTGGGAGGACGTGGCAAGGTCTACCTCTTTGACTTCCCC; GAGGGCAAGAACGCATCTGTGCGCACGGTGAATATCGGCTCCACAAAGGGGTCCTGTCTG; GATAAGCGGGACTGCGAGAACTACATCACTCTCCTGGAGAGGCGGAGTGAGGGGCTGCTG; GCCTGTGGCACCAACGCCCGGCACCCCAGCTGCTGGAACCTGGTGAATGGCACTGTGGTG; CCACTTGGCGAGATGAGAGGCTACGCCCCCTTCAGCCCGGACGAGAACTCCCTGGTTCTG; TTTGAAGGGGACGAGGTGTATTCCACCATCCNGAAGCAGGAATACAATGGGAAGATCCCT; NCGTTCCGCCGCATCCGGNGCGAGAGTGAGCTGTACACCAGTGATACTGTCATGCAGAAC; CCACAGTTCATCAAAGCCACCATCGTGCACCAAGACCCAGCTTNACGATGACAGATCTAC; TACTTCTTNCGAGAGGACATCCTGACAAGAATCCTGAGGCCTCTCTCATGTGTCCCGTGT; TGCCCCAGTGTGCCAGGGGGACCAGGTTGGGAAAGTCACTGTCAGTCTCCAGTGGGACAC; TTTTCTGGAGAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003612 unedited; CCGTGGCGCCATTGGGCGATGGTCAACTTGCCAGGGCCAGGAAAGCACTGGGGAAGGGTC; ACAGGGCTGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAG; TCGAGTTTTTTTTTTTTTTTTTTTAAGCCTAACATAAAAATAGAGCTTTTTCCGTCCTTC; CGTCCGGAAAGCAAACATCCTTCAATAAACATGCAAGGCGGCTGTCCTGTGGGACCCAGG; ACCAGAGAGGGAGCTGCAGAGGACAGGGCTGGACAGAGGGTAGCCCTGGGTCTTCAAGAA; CACCAGCCACCCAGCCATGAGAGAGGGAGGGGAAGGAGGCAATGTGGGTACCAAGAGTCC; AGAAGGACTCAGGCCTCAGCCCCAGGGTCGAGATGGAGTCCCAGCTCTCCTATCCAAACC; CACTCCCCGACCCATGGGCTCTTGGGCTGGGAGCATCGCTGCATTTAGTCAAGTTTGAGG; AGTCTGAAAAATATTTTCCAGAAGATAAAGTCTTGGGTCATCGATGCCCCAGCTTCACAG; TCGGTGCCCTCATTCTCAGCCCCTCACCATCCGTGCGCCACCTGGGGCCCAGCAGCCGCC; TGCGGCTGGACGTCTCCAGGCCTGGCATCCTCCACTGGGTTATTCTGTCCCCTGGAAGAA; GTGGCAGGCAAGGAGCTCCCGGGCCAGCCGGCTCTGAGTGTGAGACGTTCTAGTGCCCTG; GGCTGCANAAGCCTGAGGCATGCCCAGCCTCGGGAGGCCCTAGTGGACCAGCAAGCCAAG; AGTGAGTGTGGGCAGCACCCCCAGCCAGAGGGAGGCGGCCAGGGCACAGGCATGACCCAG; CAGGTGCTCGGCCATGATGCCGTCCTCGGGCAGCAGCTGCCAGTGCTGAGCCTCGCAGAA; GTAAGAGCCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003612
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003612.1, NP_003603.1
• RefSeq Size: 2661 bp
• RefSeq ORF: 2001 bp
• Locus ID: 8482
• UniProt ID: O75326
• Cytogenetics: 15q24.1
• Domains: PSI, Sema
• Protein Pathways: Axon guidance
• Summary: This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record represents the SEMA7A*001.1.1 allele.