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ALAD (NM_000031) Human Untagged Clone

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SKU
SC117775
ALAD (untagged)-Human aminolevulinate dehydratase (ALAD)
  $457.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ALAD
Synonyms ALADH; PBGS
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_000031, the custom clone sequence may differ by one or more nucleotides


ATGCAGCCCCAGTCCGTTCTGCACAGCGGCTACTTCCACCCACTACTTCGGGCCTGGCAGACAGCCACCA
CCACCCTCAATGCCTCCAACCTCATCTACCCCATCTTTGTCACGGATGTTCCTGATGACATACAGCCTAT
CACCAGCCTCCCAGGAGTGGCCAGGTATGGTGTGAAGCGGCTGGAAGAGATGCTGAGGCCCTTGGTGGAA
GAGGGCCTACGCTGTGTCTTGATCTTTGGCGTCCCCAGCAGAGTTCCCAAGGACGAGCGGGGTTCCGCAG
CTGACTCCGAGGAGTCCCCAGCTATTGAGGCAATCCATCTGTTGAGGAAGACCTTCCCCAACCTCCTGGT
GGCCTGTGATGTCTGCCTGTGTCCCTACACCTCCCATGGTCACTGCGGGCTCCTGAGTGAAAACGGAGCA
TTCCGGGCTGAGGAGAGCCGCCAGCGGCTGGCTGAGGTGGCATTGGCGTATGCCAAGGCAGGATGTCAGG
TGGTAGCCCCGTCGGACATGATGGATGGACGCGTGGAAGCCATCAAAGAGGCCCTGATGGCACATGGACT
TGGCAACAGGGTATCGGTGATGAGCTACAGTGCCAAATTTGCTTCCTGTTTCTATGGCCCTTTCCGGGAT
GCAGCTAAGTCAAGCCCAGCTTTTGGGGACCGCCGCTGCTACCAGCTGCCCCCTGGAGCACGAGGCCTGG
CTCTCCGAGCTGTGGACCGGGATGTACGGGAAGGAGCTGACATGCTCATGGTGAAGCCGGGAATGCCCTA
CCTGGACATCGTGCGGGAGGTAAAGGACAAGCACCCTGACCTCCCTCTCGCCGTGTACCACGTCTCTGGA
GAGTTTGCCATGCTGTGGCATGGAGCCCAGGCCGGGGCATTTGATCTCAAGGCTGCCGTACTGGAGGCCA
TGACTGCCTTCCGCAGAGCAGGTGCTGACATCATCATCACCTACTACACACCGCAGCTGCTGCAGTGGCT
GAAGGAGGAATGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_000031 unedited
GTCAAAATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCCAATGCCCCAG
GAGCCCTCGGTTCCAACCAACTGATGCCCCTGTGCCCACTGGCCCACGCCATGCAGCCCC
AGTCCGTTCTGCACAGCGGCTACTTCCACCCACTACTTCGGGCCTGGCAGACAGCCACCA
CCACCCTCAATGCCTCCAACCTCATCTACCCCATCTTTGTCACGGATGTTCCTGATGACA
TACAGCCTATCACCAGCCTCCCAGGAGTGGCCAGGTACGGTGTGAAGCGGCTGGAAGAGA
TGCTGAGGCCCTTGGTGGAAGAGGGCCTACGCTGTGTCTTGATCTTTGGCGTCCCCAGCA
GAGTTCCCAAGGACGAGCGGGGTTCCGCAGCTGACTCCGAGGAGTCCCCAGCTATTGAGG
CAATCCATCTGTTGAGGAAGACCTTCCCCAACCTCCTGGTGGCCTGTGATGTCTGCCTGT
GTCCCTACACCTCCCATGGTCACTGCGGGCTCCTGAGTGAAAACGGAGCATTCCGGGCTG
AGGAGAGCCGCCAGCGGCTGGCTGAGGTGGCATTGGCGTATGCCAAGGCAGGATGTCAGG
TGGTAGCCCCGTCGGACATGATGGATGGACGCGTGGAAGCCATCANAGAGGCCCTGATGG
CACATGGACTTGGCAACAGGGTATCGGTGATGAGCTACAGTGCCAAATTTTGCTTCTGTT
TCTATGGCCCTTTCCGGGATGCAGCTAAGTCAAGCCCAGCTTTTGGNGACCGNCGCTGCT
ACCAGCTNGCCCCTGGAGCACGAAGCCTGGCTCTCCGAGCTGTGGACGGGNATGTACGGG
AAGGAGCTGAATGCTCATGGTGAACCNGGNATGCCCTACCTGGACTCGTGCGGNAGGTAA
AGACAAGACCCGGACTCCTTTTCGCGGTACACGTCTTGGAAATTTGCCGA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000031 unedited
NNNNNCCCCCCCTAGGAGATGGCACTTCCAGGCCCAGAAAAGCACTGGGGNAGGGTCACA
GGNATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG
AGTTTTTTCTTTTTTTTTTATTGTACTTCAAAAATTGAATTTTTAATCAAAATTCTTTAT
AAATTTATTACAATATAGATTTTGTGATGCATACCTTTGCCCACTGCAGGCTAATGTGAG
TGTTCTGAGCAAGTTTAAGGCAGGCTAGGCTAACCTATGATGTTTGGAAGGTTAGGTATA
TTAAATACATTTTTGACTCACGATCATATTTTCAACTTATGATCACATTTTCAACTTAGG
ACGGGTTTATCAGGATGCAGACCCATCACATAAATGGAGGGGTGTCCATAAGACATTGCT
AGACCTATAAATTTGCTGTTGAATAATGGGAGGTGGTATGGCACAGTGGTTAGGGGCATA
GGCCTTGGAGTTGGACTACATGGGTTCAAATCCCAGCTTGGCTGTTTTCTGTGCAGTTCT
AATCCAGTTCTGCCACAAACTTGTTGAGTGGCCTTAAGTGACTCACATTAGCACTCTAGA
TATAGCTTCCTTATGTGGAAAAGGCGCAAGCAAACTTTAGTATTCTCCCTCCACCCCGAC
CATGCTTCTGTTCCTCATCCAGGCTGCCCCATCTTAGTAAATGGACCATCAACCACCGCG
AAGTCAAGCCAAATTAATCCTTCACATCGGATCTACCTTCTAAACACATCCCCGGTCCCA
TTTCCCTTTCTCCATCGCACTGTCCCCGGTCCGGTCCATGACAAGAAGAATTTTCAAT
Restriction Sites NotI-NotI |
ACCN NM_000031
Insert Size 3700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000031.4, NP_000022.2
RefSeq Size 3151 bp
RefSeq ORF 1020 bp
Locus ID 210
UniProt ID P13716
Cytogenetics 9q32
Domains ALAD
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Porphyrin and chlorophyll metabolism
Summary The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. provided by RefSeq, Dec 2015
Transcript Variant: This variant (2) lacks a segment of the coding region and initiates translation from an alternate start codon, compared to variant 1. The resulting isoform (b) has a distinct N-terminus, compared to isoform a.
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Documents
Resources
"NM_000031" in other vectors (6)
SKU Description Size Price
RC201545 ALAD (Myc-DDK-tagged)-Human aminolevulinate dehydratase (ALAD) 10 ug
$457.00
RC201545L1 Lenti ORF clone of Human aminolevulinate dehydratase (ALAD), Myc-DDK-tagged 10 ug
$757.00
RC201545L2 Lenti ORF clone of Human aminolevulinate dehydratase (ALAD), mGFP tagged 10 ug
$757.00
RC201545L3 Lenti ORF clone of Human aminolevulinate dehydratase (ALAD), Myc-DDK-tagged 10 ug
$757.00
RC201545L4 Lenti ORF clone of Human aminolevulinate dehydratase (ALAD), mGFP tagged 10 ug
$757.00
RG201545 ALAD (tGFP-tagged) - Human aminolevulinate dehydratase (ALAD) 10 ug
$657.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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