MRPL40 (NM_003776) Human Untagged Clone

SKU
SC117758
MRPL40 (untagged)-Human mitochondrial ribosomal protein L40 (MRPL40), nuclear gene encoding mitochondrial protein
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MRPL40
Synonyms L40mt; MRP-L22; MRP-L40; MRPL22; NLVCF; URIM
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117758 sequence for NM_003776 edited (data generated by NextGen Sequencing)
ATGACGGCCTCCGTGCTGCGAAGTATCTCGCTAGCCCTGCGCCCGACTAGCGGGCTTCTG
GGAACTTGGCAGACGCAGCTTAGAGAGACTCACCAGCGAGCGTCATTGTTGTCTTTCTGG
GAACTCATTCCCATGAGATCAGAACCTCTTCGAAAAAAGAAGAAGGTAGATCCTAAAAAA
GACCAAGAAGCAAAGGAGCGCTTGAAAAGGAAGATCCGAAAACTGGAAAAGGCTACTCAA
GAGCTAATTCCTATTGAAGATTTTATTACCCCTCTAAAGTTCTTGGATAAAGCAAGAGAG
CGGCCTCAGGTGGAGCTCACCTTTGAGGAGACTGAGAGGAGAGCTCTGCTTCTGAAGAAG
TGGTCCTTGTACAAGCAGCAAGAGCGTAAGATGGAGAGGGACACCATCAGGGCTATGCTA
GAAGCCCAGCAGGAAGCTCTGGAGGAACTGCAACTGGAATCCCCGAAGCTCCATGCTGAG
GCCATCAAGCGGGATCCTAACCTGTTCCCCTTTGAGAAGGAAGGGCCACATTACACACCA
CCGATCCCTAACTACCAACCCCCTGAAGGCAGGTACAATGACATCACCAAGGTGTACACA
CAAGTGGAGTTTAAGAGATAG

Clone variation with respect to NM_003776.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003776 unedited
GTTCGTCACCCGCCCGTTGCCGTTATAGGGCGTAGGCGTGTACGGTGGNGAGTCTATATA
AGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCG
CGAATCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGGTGGAGGGGCGCACGCCCGGA
AGCGGCGAGGGTAGCCATGACGGCCTCCGTGCTGCGAAGTATCTCGCTAGCCCTGCGCCC
GACTAGCGGGCTTCTGGGAACTTGGCAGACGCAGCTTAGAGAGACTCACCAGCGAGCGTC
ATTGTTGTCTTTCTGGGAACTCATTCCCATGAGATCAGAACCTCTTCGAAAAAAGAAGAA
GGTAGATCCTAAAAAAGACCAAGAAGCANAGGAGCGCTTGANAAGGAAGATCCGAANACT
GGAAAAGGCTACTCAAGAGCTAATTCCTATTGAAGATTTTATTACCCCTCTAAAGTTCTT
GGATAAAGCAAGAGAGCGGCCTCAGGTGGAGCTCACCTTTGAGGAGACTGAGAGGAGAGC
TCTGCTTCTGAAGAAGTGTCCTTGTACAAGCAGCAAGAGCGTAAGATGGAGAGGGACACC
ATCAGGCCTATGCTAGAAGCCCAGCAAGAAGCTCTGGAGGAACTGCCACTGAATCCCCCG
AGCTCCTTGCTGGAGCCATCAAGCGGGATCCTAAACCTGTTCCCCTTTGGAAAGGGAGGG
NCCCAATAACAAACAACGGTCCCTAATTACAACCCCCTGGAAGGCGGTCCATGGCTTCCC
CAAGGTGACCAACCAATGGAGTTTAAGAAAATAAATTTGGAGGTTGCTTATCTTAAAAAT
GCTGGCCCTGGAAATAGGGAATGACCAGGGTTTAAGATTTGTTTTCAAAAAATAAGGGTG
GCT
Restriction Sites NotI-NotI |
ACCN NM_003776
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003776.2, NP_003767.2
RefSeq Size 787 bp
RefSeq ORF 621 bp
Locus ID 64976
UniProt ID Q9NQ50
Cytogenetics 22q11.21
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Deletions in this gene may contribute to the etiology of velo-cardio-facial syndrome and DiGeorge syndrome. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_003776" in other vectors (5)
SKU Description Size Price
RC202166 MRPL40 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L40 (MRPL40), nuclear gene encoding mitochondrial protein 10 ug
$300.00
RC202166L3 Lenti ORF clone of Human mitochondrial ribosomal protein L40 (MRPL40), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$600.00
RC202166L4 Lenti ORF clone of Human mitochondrial ribosomal protein L40 (MRPL40), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$600.00
RG202166 MRPL40 (tGFP-tagged) - Human mitochondrial ribosomal protein L40 (MRPL40), nuclear gene encoding mitochondrial protein 10 ug
$500.00
SC322398 MRPL40 (untagged)-Human mitochondrial ribosomal protein L40 (MRPL40), nuclear gene encoding mitochondrial protein 10 ug
$300.00

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