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Apc6 (CDC16) (NM_003903) Human Untagged Clone

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SKU
SC117672
CDC16 (untagged)-Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 1
  $577.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Apc6
Synonyms ANAPC6; APC6; CDC16Hs; CUT9
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117672 sequence for NM_003903 edited (data generated by NextGen Sequencing)
ATGAACCTAGAGCGGCTGCGGAAGCGCGTCCGGCAGTACCTCGACCAGCAACAGTATCAA
AGTGCTCTATTTTGGGCAGATAAAGTAGCTTCACTCTCTCGTGAAGAACCCCAGGACATC
TATTGGTTGGCTCAGTGTCTTTACCTGACAGCACAATATCACAGAGCCGCCCATGCACTT
CGGTCACGAAAACTGGACAAATTGTATGAAGCATGTCGTTACCTTGCAGCTAGGTGCCAT
TATGCTGCAAAAGAGCACCAGCAGGCCCTTGATGTTCTTGACATGGAAGAGCCCATCAAT
AAAAGATTATTTGAAAAATACTTGAAGGATGAAAGTGGCTTCAAAGATCCCTCCAGCGAC
TGGGAAATGTCACAGTCTTCAATAAAGAGTTCTATCTGTCTTCTACGCGGGAAAATCTAT
GATGCTCTAGATAACCGAACCCTGGCTACCTACAGCTACAAAGAAGCTTTGAAGCTTGAT
GTCTACTGTTTTGAAGCGTTCGATCTTTTAACATCACATCACATGCTGACAGCACAAGAA
GAAAAAGAACTTCTTGAATCACTACCCCTTAGCAAGCTGTGTAATGAAGAACAGGAATTG
CTGCGTTTTCTATTTGAGAACAAATTGAAAAAATATAATAAGCCTAGTGAAACGGTCATC
CCTGAATCTGTAGATGGCTTGCAAGAGAATCTGGATGTGGTAGTGTCTTTAGCTGAGAGA
CATTATTATAACTGTGATTTTAAAATGTGCTACAAGCTTACTTCTGTAGTAATGGAGAAA
GATCCTTTCCATGCAAGTTGTTTACCTGTACATATAGGGACGCTTGTAGAGCTGAATAAA
GCCAATGAACTTTTCTATCTTTCTCATAAACTGGTGGATTTATATCCTAGTAATCCTGTG
TCTTGGTTTGCAGTGGGATGTTACTATCTCATGGTCGGTCATAAAAATGAACATGCCAGA
AGATATCTCAGCAAAGCCACAACACTTGAGAAAACCTATGGACCTGCATGGATAGCCTAT
GGACATTCATTTGCGGTGGAGAGTGAGCACGACCAAGCGATGGCTGCTTACTTCACAGCA
GCACAGCTGATGAAAGGGTGTCATTTGCCTATGCTGTATATTGGATTAGAATATGGTTTG
ACCAATAACTCAAAACTAGCTGAAAGGTTCTTCAGCCAAGCTCTGAGCATTGCACCGGAA
GACCCTTTTGTTATGCATGAGGTCGGCGTGGTTGCATTTCAGAATGGAGAATGGAAAACA
GCCGAAAAATGGTTTCTTGATGCTTTGGAAAAAATTAAAGCAATTGGGAACGAGGTAACA
GTTGACAAATGGGAACCTTTGTTGAACAACTTGGGGCATGTCTGCAGAAAACTTAAAAAG
TATGCTGAGGCCTTGGATTACCACCGTCAGGCACTGGTGTTGATTCCTCAGAACGCATCC
ACCTACTCTGCTATTGGATATATCCACAGTCTGATGGGCAACTTTGAAAATGCTGTGGAC
TACTTCCACACAGCCCTTGGTCTTAGGCGAGATGATACATTTTCTGTTACAATGCTTGGT
CATTGCATCGAAATGTACATTGGTGATTCTGAAGCTTATATTGGAGCAGACATTAAAGAC
AAATTAAAATGTTATGACTTTGATGTGCATACAATGAAGACACTAAAAAACATTATTTCA
CCTCCGTGGGATTTCAGGGAATTTGAAGTAGAAAAACAGACTGCAGAAGAAACGGGGCTT
ACGCCATTGGAAACCTCAAGGAAAACTCCAGATTCCAGACCTTCCTTGGAAGAAACCTTT
GAAATTGAAATGAATGAAAGTGACATGATGTTAGAGACATCTATGTCAGACCACAGCACG
TGA

Clone variation with respect to NM_003903.3
330 c=>t;351 t=>c
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003903 unedited
GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACCTGCGGCC
TTCGAGTCCGCGGCCTTCGAGTCCTGGGGCGGCGGCGGCGGCTGCAGGCACGGGCACGGG
CACGGGGCGGGGTGCTTAGGGTGCAGGAGGCGCGCGCCTAGCGGCGGAGTGTGGCGTGAG
GCCGGGCCCGCGCCGCCATGAACCTAGAGCGGCTGCGGAAGCGCGTCCGGCAGTACCTCG
ACCAGCAACAGTATCAAAGTGCTCTATTTTGGGCAGATAAAGTAGCTTCACTCTCTCGTG
AAGAACCCCAGGACATCTATTGGTTGGCTCAGTGTCTTTACCTGACAGCACAATATCACA
GAGCCGCCCATGCACTTCGGTCACGAAAACTGGACAAATTGTATGAAGCATGTCGTTACC
TTGCAGCTAGGTGCCATTATGCTGCAAAAGAGCACCAGCAGGCCCTTGATGTTCTTGACA
TGGAAGAGCCCATCAATAAAAGATTATTTGAAAAATACTTGAAGGATGAAAGTGGCTTCA
AAGATCCCTCCAGCGACTGGGAAATGTCACAGTCTTCAATAAAGAGTTCTATCTGTCTTC
TACGCGGGAAAATCTATGATGCTCTAGATAACCGAACCCTGGCTACCTACAGCTACAAAG
AAGCTNTGAAGCTTGATGTCTACTGTTTTGAAAGCGTCGATCTTTTAACATCACATCACA
TGCTGACAGCACAAGAAGAAAAGAACTTCTTTGATCACTACCCTTNNAGCAGCTGTGTAA
TGAAGAAACAGAATTGCTGCCGTNTCTATTTGAGAACNNAATGAAAAATATATAAGCCTA
GTGAAACGGTCATCCTGATCTGTANATGGCTGCAGAGAATCTGGTGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003903 unedited
TGAACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAGGGGTACCTTTTTTTTTCCACTTTGTATA
ACTATTTTGGGGGGTCAAAAAATCAAAACCCATTTGTTGCAAAGGGGGGCCAACCAATCT
TTTAAGGGGGGTCTTTTTTCCTACCCTGGGACAGGGGGACCCAGACCCCTGGCTGGGGTC
ACCTGCTGGGGGCTGACATAAATGTCTTTAACATTATGGGACTTTTATTTATTTCAATTT
CAAAGGGTTTTTCCAAGGAAGGGCTGGAATTTGGAGTTTTCCTTGAGGGTTCCAAAGGGG
TAAACCCCGTTTTTTTTGCAGGCTGTTTTTTTACTTCAAATTCCCTGAAATCCCACGGGG
GGGAAAAAATGGTTTTTAGGGGCTTCATTGGGTGCCCCTCAAAGGCATAACATTTTAATT
TGGCTTTAAAGGCCGCTCCAAAATAAACTTCAAAAACACCAATGTACATTTCGATGCAAT
GACCAAACATTGTAACCAAAAATGTATTATTTCTCCTAAAACCACAGGGCCTGTGGGGGA
AGAACTCCACACCCTTTTTAAAAGTGCCCCTCAAACCGGGGGATTTATCCCAAACCACAA
AAGGGGGGGTCCGTTTTGAGGAATCAACCCCCAGGCCTGGCGGTGGGAATTCCAAGGCCT
CAAAATTATCTTATAAATTTTTTTGCAAAACTGCCCCACAGTGTTCAACAAGAGTTCCCT
TTTTGGAAACGTGTAACCCGCTCCCCCATTGGTTTTAATTTTTTCCAAAACATAAAGAAA
CCTTTTTTGGCTGTGTTTCCATTTCCACTTTCGAAAGCCCCCCCCCCCCCCTCTGCCTAA
AAAAAGGGGTT
Restriction Sites NotI-NotI |
ACCN NM_003903
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003903.2, NP_003894.2
RefSeq Size 2196 bp
RefSeq ORF 1860 bp
Locus ID 8881
UniProt ID Q13042
Cytogenetics 13q34
Domains TPR
Protein Families Druggable Genome
Protein Pathways Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
Summary The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. provided by RefSeq, Jan 2016
Transcript Variant: This variant (1) lacks an exon segment in the 3' UTR, compared to variant 2. Variants 1 and 2 encode the same isoform (1).
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Documents
Resources
"NM_003903" in other vectors (7)
SKU Description Size Price
RC205639 CDC16 (Myc-DDK-tagged)-Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 1 10 ug
$577.00
RC205639L1 Lenti ORF clone of Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 1, Myc-DDK-tagged 10 ug
$877.00
RC205639L2 Lenti ORF clone of Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 1, mGFP tagged 10 ug
$877.00
RC205639L3 Lenti ORF clone of Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 1, Myc-DDK-tagged 10 ug
$877.00
RC205639L4 Lenti ORF clone of Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 1, mGFP tagged 10 ug
$877.00
RG205639 CDC16 (tGFP-tagged) - Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 1 10 ug
$489.00 $777.00
SC318066 CDC16 (untagged)-Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 1 10 ug
$636.00

file-alt Citations

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