H6PD (NM_004285) Human Untagged Clone

SKU SC117481

H6PD (untagged)-Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD)

Product Data

• Type: Human Untagged Clone
• Target Symbol: H6PD
• Synonyms: CORTRD1; G6PDH; GDH; H6PDH
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004285 edited; GGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTATA; GAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATCGGCACGAGGAGCCTTTTAAC; TGGCACCCAGGCATGTGGAATATGCTCATAGTGGCGATGTGCTTGGCCCTTCTGGGCTGC; CTGCAAGCCCAGGAGCTCCAGGGACATGTCTCCATAATCCTGCTGGGAGCAACTGGGGAC; CTGGCTAAGAAGTACTTATGGCAGGGACTGTTCCAGCTGTACCTGGATGAAGCGGGGAGG; GGTCACAGTTTTAGCTTCCATGGAGCTGCTCTGACAGCCCCCAAGCAGGGTCAAGAGCTC; ATGGCCAAGGCCCTGGAATCCCTCTCCTGCCCCAAGGACATGGCACCCAGTCACTGTGCA; GAGCACAAGGATCAGTTCCTGCAGCTGAGCCAGTACCGCCAACTGAAGACGGCCGAGGAC; TATCAGGCCCTGAACAAGGACATCGAGGCACAGCTCCAGCACGCAGGCCTCCGGGAGGCT; GGCAGGATCTTCTACTTCTCAGTGCCACCCTTCGCCTATGAAGACATTGCCCGCAACATC; AACAGTAGCTGCCGGCCAGGCCCGGGCGCCTGGCTGCGGGTTGTCCTTGAGAAACCCTTT; GGCCATGACCACTTCTCAGCCCAGCAGCTGGCCACAGAACTCGGGACCTTTTTCCAGGAG; GAGGAGATGTACCGGGTGGACCATTACTTAGGCAAGCAGGCTGTGGCGCAGATCCTGCCT; TTCCGAGACCAGAACCGCAAGGCTTTGGACGGCCTCTGGAACCGGCACCATGTGGAGCGG; GTGGAGATCATCATGAAAGAGACCGTGGATGCTGAAGGCCGCACCAGCTTCTATGAGGAG; TACGGTGTCATTCGCGACGTCCTCCAGAACCATCTGACGGAGGTCCTCACCCTCGTGGCC; ATGGAGCTGCCCCACAATGTCAGCAGTGCGGAGGCTGTGCTGCGGCACAAGCTTCAGGTC; TTCCAGGCGCTGCGGGGCCTGCAGAGGGGCAGTGCCGTCGTGGGCCAGTACCAGTCTTAC; AGTGAGCAGGTGCGCAGAGAGCTGCAGAAGCCAGACAGCTTCCACAGCCTGACGCCGACC; TTCGCAGCCGTCCTAGTGCACATTGACAACCTTCGCTGGGAGGGCGTGCCTTTCATCCTG; ATGTCTGGCAAAGCCTTGGACGAGAGAGTGGGCTACGCTCGGATCTTGTTCAAGAACCAG; GCCTGCTGTGTGCAGAGCGAAAAGCACTGGGCCGCGGCGCAGAGCCAGTGCCTGCCCCGG; CAGCTCGTCTTCCACATCGGCCATGGCGACCTGGGCAGCCCTGCCGTGCTGGTCAGCAGG; AACCTGTTCAGGCCCTCCCTGCCCTCCAGCTGGAAGGAAATGGAGGGACCACCTGGGCTC; CGCCTTTTCGGCAGCCCTCTGTCCGATTACTACGCCTACAGCCCTGTGCGGGAGCGGGAC; GCCCACTCCGTCCTCTTATCCCATATCTTCCATGGCCGGAAGAATTTCTTCATCACCACA; GAGAACTTGCTGGCCTCCTGGAACTTCTGGACCCCTCTGCTGGAGAGCCTGGCCCATAAG; GCCCCACGCCTCTACCCTGGAGGAGCTGAGAATGGCCGTCTGTTGGACTTTGAGTTCAGT; AGCGGCCGGTTGTTCTTTTCCCAGCAGCAGCCGGAGCAGCTGGTGCCAGGGCCAGGGCCG; GCCCCAATGCCCAGTGACTTCCAGGTCCTCAGGGCCAAGTACCGAGAGAGCCCGCTGGTC; TCCGCCTGGTCCGAGGAGCTGATCTCTAAGCTGGCTAATGACATCGAGGCCACCGCTGTG; CGAGCCGTGCGGCGCTTTGGCCAGTTCCACCTGGCACTGTCGGGGGGCTCGAGCCCCGTG; GCCCTGTTCCAGCAGCTGGCCACGGCGCACTATGGCTTCCCCTGGGCCCACACGCACCTG; TGGCTGGTTGACGAGCGCTGCGTCCCACTCTCAGACCCGGAGTCCAACTTCCAGGGCCTG; CAGGCCCACCTGCTGCAGCACGTCCGGATCCCCTACTACAACATCCACCCCATGCCTGTG; CACCTGCAGCAGCGGCTCTGCGCCGAGGAGGACCAGGGCGCCCAGATCTATGCCAGGGAG; ATCTCAGCCCTGGTGGCCAACAGCAGCTTCGACCTGGTGCTGCTGGGCATGGGTGCCGAC; GGGCACACAGCCTCCCTCTTCCCACAGTCACCCACTGGCCTGGATGGCGAGCAGCTGGTC; GTGCTGACCACGAGCCCCTCCCAGCCACACCGCCGCATGAGCCTTAGCCTGCCTCTCATC; AACCGCGCCAAGAAGGTGGCAGTCCTGGTCATGGGCAGGATGAAGCGTGAGATCACCACG; CTGGTGAGCCGGGTGGGCCATGAGCCCAAGAAGTGGCCCATCTCGGGTGTCCTGCCGCAC; TCCGGCCAGCTGGTGTGGTACATGGACTACGACGCCTTCCTGGGATGAGGGCGCCTGTGC; CCCTTGCCCGCTTCGCTCCTGTGCTTTCCTTCGCCCGTGTCTTCCCTCCCTTCTCGGCCC; CGCCACCTGCCCAGCGTGCCCTGGCTCTCCAGAACCTTCTATCCCACAGTCAGGCCCCAG; AGAGGGCAGGACAAGCCTTGTCCCGATGCCTTTGACCGGCAGCTCTGTGTATTGGTGGAT; AGATGCAGAAACAAGGAAGAAATGGAGTCTGCTCCTGAGAAGCTTCAAATTCAGGCCAGG; AGAGAAGTCTTAAGAAAAGACCTCCAGCAGTTACACATTCATATCAACCAGCACAACACG; GGATGGCGCCCAAACTCCGGCGTTCACAAGAGGAGACGTGACGTGGTGGGCTGAGGTTAA; TCAGGGAAGGTTTCCTGGGGGAGGTGATCCTTGAACTGGCTCCCGGGGAACATTCAGAGC; ATGATTGGTAGACAGAAGGGTGCAGAGGCGCCCAGGGGAGTACATTGCCCCGTGC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004285 unedited; CGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT; CATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATCGG; CACGAGGAGCCTTTTAACTGGCACCCAGGCATGTGGAATATGCTCATAGTGGCGATGTGC; TTGGCCCTTCTGGGCTGCCTGCAAGCCCAGGAGCTCCAGGGACATGTCTCCATAATCCTG; CTGGGAGCAACTGGGGACCTGGCTAAGAAGTACTTATGGCAGGGACTGTTCCAGCTGTAC; CTGGATGAAGCGGGGAGGGGTCACAGTTTTAGCTTCCATGGAGCTGCTCTGACAGCCCCC; AAGCAGGGTCAAGAGCTCATGGCCAAGGCCCTGGAATCCCTCTCCTGCCCCAAGGACATG; GCACCCAGTCACTGTGCAGAGCACAAGGATCAGTTCCTGCAGCTGAGCCAGTACCGCCAA; CTGAAGACGGCCGAGGACTATCAGGCCCTGAACAAGGACATCGAGGCACAGCTCCAGCAC; GCAGGCCTCCGGGAGGCTGGCAGGATCTTCTACTTCTCAGTGCCACCCTTCGCCTATGAA; GACATTGCCCGCAACATCAACAGTAGCTGCCGGCCAGGCCCGGGCGCCTGGCTGCGGGTT; GTCCTTGAGAAACCCTTTGGCCATGACCACTTCTCAGCCCAGCAGCTGGCCACAGAAACT; CGGACCTTNTTCCAGNAGGAGGAGATGTACCCGGTGGACCATTACTTANNGCAGCAGGCT; GTGGCGCAGATCCTGCCTTTNCGAGACCAGACCGNCAGGCTNTGNACGGNCTCTGNAACC; NGCACATGTGNAGCGGNTGGAGNACATCATGAAAGAGACCGTGATGCTGAAGGCGCACAG; CTCTATGAGAGTACGTGTCATTCCGACGTCTCCAGACATCTGACGAGGTCTNACCTCGTG; GCTGNACTGCCACATGTCACATGCGGAGCTGGCTGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004285
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004285.3, NP_004276.2
• RefSeq Size: 9117 bp
• RefSeq ORF: 2376 bp
• Locus ID: 9563
• UniProt ID: O95479
• Cytogenetics: 1p36.22
• Domains: G6PD, Glucosamine_iso
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway
• Summary: There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) uses an alternate 5' most exon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.