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H6PD (NM_004285) Human Untagged Clone

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SKU
SC117481
H6PD (untagged)-Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD)
  $725.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol H6PD
Synonyms CORTRD1; G6PDH; GDH; H6PDH
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_004285 edited
GGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTATA
GAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATCGGCACGAGGAGCCTTTTAAC
TGGCACCCAGGCATGTGGAATATGCTCATAGTGGCGATGTGCTTGGCCCTTCTGGGCTGC
CTGCAAGCCCAGGAGCTCCAGGGACATGTCTCCATAATCCTGCTGGGAGCAACTGGGGAC
CTGGCTAAGAAGTACTTATGGCAGGGACTGTTCCAGCTGTACCTGGATGAAGCGGGGAGG
GGTCACAGTTTTAGCTTCCATGGAGCTGCTCTGACAGCCCCCAAGCAGGGTCAAGAGCTC
ATGGCCAAGGCCCTGGAATCCCTCTCCTGCCCCAAGGACATGGCACCCAGTCACTGTGCA
GAGCACAAGGATCAGTTCCTGCAGCTGAGCCAGTACCGCCAACTGAAGACGGCCGAGGAC
TATCAGGCCCTGAACAAGGACATCGAGGCACAGCTCCAGCACGCAGGCCTCCGGGAGGCT
GGCAGGATCTTCTACTTCTCAGTGCCACCCTTCGCCTATGAAGACATTGCCCGCAACATC
AACAGTAGCTGCCGGCCAGGCCCGGGCGCCTGGCTGCGGGTTGTCCTTGAGAAACCCTTT
GGCCATGACCACTTCTCAGCCCAGCAGCTGGCCACAGAACTCGGGACCTTTTTCCAGGAG
GAGGAGATGTACCGGGTGGACCATTACTTAGGCAAGCAGGCTGTGGCGCAGATCCTGCCT
TTCCGAGACCAGAACCGCAAGGCTTTGGACGGCCTCTGGAACCGGCACCATGTGGAGCGG
GTGGAGATCATCATGAAAGAGACCGTGGATGCTGAAGGCCGCACCAGCTTCTATGAGGAG
TACGGTGTCATTCGCGACGTCCTCCAGAACCATCTGACGGAGGTCCTCACCCTCGTGGCC
ATGGAGCTGCCCCACAATGTCAGCAGTGCGGAGGCTGTGCTGCGGCACAAGCTTCAGGTC
TTCCAGGCGCTGCGGGGCCTGCAGAGGGGCAGTGCCGTCGTGGGCCAGTACCAGTCTTAC
AGTGAGCAGGTGCGCAGAGAGCTGCAGAAGCCAGACAGCTTCCACAGCCTGACGCCGACC
TTCGCAGCCGTCCTAGTGCACATTGACAACCTTCGCTGGGAGGGCGTGCCTTTCATCCTG
ATGTCTGGCAAAGCCTTGGACGAGAGAGTGGGCTACGCTCGGATCTTGTTCAAGAACCAG
GCCTGCTGTGTGCAGAGCGAAAAGCACTGGGCCGCGGCGCAGAGCCAGTGCCTGCCCCGG
CAGCTCGTCTTCCACATCGGCCATGGCGACCTGGGCAGCCCTGCCGTGCTGGTCAGCAGG
AACCTGTTCAGGCCCTCCCTGCCCTCCAGCTGGAAGGAAATGGAGGGACCACCTGGGCTC
CGCCTTTTCGGCAGCCCTCTGTCCGATTACTACGCCTACAGCCCTGTGCGGGAGCGGGAC
GCCCACTCCGTCCTCTTATCCCATATCTTCCATGGCCGGAAGAATTTCTTCATCACCACA
GAGAACTTGCTGGCCTCCTGGAACTTCTGGACCCCTCTGCTGGAGAGCCTGGCCCATAAG
GCCCCACGCCTCTACCCTGGAGGAGCTGAGAATGGCCGTCTGTTGGACTTTGAGTTCAGT
AGCGGCCGGTTGTTCTTTTCCCAGCAGCAGCCGGAGCAGCTGGTGCCAGGGCCAGGGCCG
GCCCCAATGCCCAGTGACTTCCAGGTCCTCAGGGCCAAGTACCGAGAGAGCCCGCTGGTC
TCCGCCTGGTCCGAGGAGCTGATCTCTAAGCTGGCTAATGACATCGAGGCCACCGCTGTG
CGAGCCGTGCGGCGCTTTGGCCAGTTCCACCTGGCACTGTCGGGGGGCTCGAGCCCCGTG
GCCCTGTTCCAGCAGCTGGCCACGGCGCACTATGGCTTCCCCTGGGCCCACACGCACCTG
TGGCTGGTTGACGAGCGCTGCGTCCCACTCTCAGACCCGGAGTCCAACTTCCAGGGCCTG
CAGGCCCACCTGCTGCAGCACGTCCGGATCCCCTACTACAACATCCACCCCATGCCTGTG
CACCTGCAGCAGCGGCTCTGCGCCGAGGAGGACCAGGGCGCCCAGATCTATGCCAGGGAG
ATCTCAGCCCTGGTGGCCAACAGCAGCTTCGACCTGGTGCTGCTGGGCATGGGTGCCGAC
GGGCACACAGCCTCCCTCTTCCCACAGTCACCCACTGGCCTGGATGGCGAGCAGCTGGTC
GTGCTGACCACGAGCCCCTCCCAGCCACACCGCCGCATGAGCCTTAGCCTGCCTCTCATC
AACCGCGCCAAGAAGGTGGCAGTCCTGGTCATGGGCAGGATGAAGCGTGAGATCACCACG
CTGGTGAGCCGGGTGGGCCATGAGCCCAAGAAGTGGCCCATCTCGGGTGTCCTGCCGCAC
TCCGGCCAGCTGGTGTGGTACATGGACTACGACGCCTTCCTGGGATGAGGGCGCCTGTGC
CCCTTGCCCGCTTCGCTCCTGTGCTTTCCTTCGCCCGTGTCTTCCCTCCCTTCTCGGCCC
CGCCACCTGCCCAGCGTGCCCTGGCTCTCCAGAACCTTCTATCCCACAGTCAGGCCCCAG
AGAGGGCAGGACAAGCCTTGTCCCGATGCCTTTGACCGGCAGCTCTGTGTATTGGTGGAT
AGATGCAGAAACAAGGAAGAAATGGAGTCTGCTCCTGAGAAGCTTCAAATTCAGGCCAGG
AGAGAAGTCTTAAGAAAAGACCTCCAGCAGTTACACATTCATATCAACCAGCACAACACG
GGATGGCGCCCAAACTCCGGCGTTCACAAGAGGAGACGTGACGTGGTGGGCTGAGGTTAA
TCAGGGAAGGTTTCCTGGGGGAGGTGATCCTTGAACTGGCTCCCGGGGAACATTCAGAGC
ATGATTGGTAGACAGAAGGGTGCAGAGGCGCCCAGGGGAGTACATTGCCCCGTGC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004285 unedited
CGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT
CATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATCGG
CACGAGGAGCCTTTTAACTGGCACCCAGGCATGTGGAATATGCTCATAGTGGCGATGTGC
TTGGCCCTTCTGGGCTGCCTGCAAGCCCAGGAGCTCCAGGGACATGTCTCCATAATCCTG
CTGGGAGCAACTGGGGACCTGGCTAAGAAGTACTTATGGCAGGGACTGTTCCAGCTGTAC
CTGGATGAAGCGGGGAGGGGTCACAGTTTTAGCTTCCATGGAGCTGCTCTGACAGCCCCC
AAGCAGGGTCAAGAGCTCATGGCCAAGGCCCTGGAATCCCTCTCCTGCCCCAAGGACATG
GCACCCAGTCACTGTGCAGAGCACAAGGATCAGTTCCTGCAGCTGAGCCAGTACCGCCAA
CTGAAGACGGCCGAGGACTATCAGGCCCTGAACAAGGACATCGAGGCACAGCTCCAGCAC
GCAGGCCTCCGGGAGGCTGGCAGGATCTTCTACTTCTCAGTGCCACCCTTCGCCTATGAA
GACATTGCCCGCAACATCAACAGTAGCTGCCGGCCAGGCCCGGGCGCCTGGCTGCGGGTT
GTCCTTGAGAAACCCTTTGGCCATGACCACTTCTCAGCCCAGCAGCTGGCCACAGAAACT
CGGACCTTNTTCCAGNAGGAGGAGATGTACCCGGTGGACCATTACTTANNGCAGCAGGCT
GTGGCGCAGATCCTGCCTTTNCGAGACCAGACCGNCAGGCTNTGNACGGNCTCTGNAACC
NGCACATGTGNAGCGGNTGGAGNACATCATGAAAGAGACCGTGATGCTGAAGGCGCACAG
CTCTATGAGAGTACGTGTCATTCCGACGTCTCCAGACATCTGACGAGGTCTNACCTCGTG
GCTGNACTGCCACATGTCACATGCGGAGCTGGCTGG
Restriction Sites NotI-NotI |
ACCN NM_004285
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004285.3, NP_004276.2
RefSeq Size 9117 bp
RefSeq ORF 2376 bp
Locus ID 9563
UniProt ID O95479
Cytogenetics 1p36.22
Domains G6PD, Glucosamine_iso
Protein Pathways Metabolic pathways, Pentose phosphate pathway
Summary There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) uses an alternate 5' most exon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.
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Documents
Resources
"NM_004285" in other vectors (6)
SKU Description Size Price
RC209890 H6PD (Myc-DDK-tagged)-Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD) 10 ug
$1,086.00
RC209890L1 Lenti ORF clone of Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), Myc-DDK-tagged 10 ug
$1,386.00
RC209890L2 Lenti ORF clone of Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), mGFP tagged 10 ug
$1,386.00
RC209890L3 Lenti ORF clone of Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), Myc-DDK-tagged 10 ug
$1,386.00
RC209890L4 Lenti ORF clone of Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), mGFP tagged 10 ug
$1,386.00
RG209890 H6PD (tGFP-tagged) - Human hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD) 10 ug
$1,286.00

file-alt Citations

Identification of a tumor-promoter cholesterol metabolite in human breast cancers acting through the glucocorticoid receptor
Voisin, de Medina, Mallinger et al
PNAS (2017) 114 (44), E9346-E9355 DOI: 10.1073/pnas.1707965114
AI Summary: OriGene contributed to this study by providing plasmids for 11HSD1 (sc109325), 11HSD2 (sc122552 or RG207796), H6PD (sc117481), DHCR7 (sc110871), and D8D7I (EBP), which were used to investigate the roles of these enzymes in cholesterol metabolism and breast cancer progression.
Snippet: The NEON Transfection system was purchased from Invitrogen, the BrdU cell proliferation Elisa kit was from Roche Diagnostics or Sigma, and the following plasmids were from OriGene: 11HSD1: sc109325;11HSD2: sc122552 or RG207796;H6PD: sc117481;DHCR7: sc110871;and D8D7I (EBP):
Showing 1-1 of 1 papers.
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