AGL (NM_000028) Human Untagged Clone

SKU SC117442

AGL (untagged)-Human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: AGL
• Synonyms: GDE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000028, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGGACACAGTAAACAGATTCGAATTTTACTTCTGAACGAAATGGAGAAACTGGAAAAGACCCTCTTCA; GACTTGAACAAGGGTATGAGCTACAGTTCCGATTAGGCCCAACTTTACAGGGAAAAGCAGTTACCGTGTA; TACAAATTACCCATTTCCTGGAGAAACATTTAATAGAGAAAAATTCCGTTCTCTGGATTGGGAAAATCCA; ACAGAAAGAGAAGATGATTCTGATAAATACTGTAAACTTAATCTGCAACAATCTGGTTCATTTCAGTATT; ATTTCCTTCAAGGAAATGAGAAAAGTGGTGGAGGTTACATAGTTGTGGACCCCATTTTACGTGTTGGTGC; TGATAATCATGTGCTACCCTTGGACTGTGTTACTCTTCAGACATTTTTAGCTAAGTGTTTGGGACCTTTT; GATGAATGGGAAAGCAGACTTAGGGTTGCAAAAGAATCAGGCTACAACATGATTCATTTTACCCCATTGC; AGACTCTTGGACTATCTAGGTCATGCTACTCCCTTGCCAATCAGTTAGAATTAAATCCTGACTTTTCAAG; ACCTAATAGAAAGTATACCTGGAATGATGTTGGACAGCTAGTGGAAAAATTAAAAAAGGAATGGAATGTT; ATTTGTATTACTGATGTTGTCTACAATCATACTGCTGCTAATAGTAAATGGATCCAGGAACATCCAGAAT; GTGCCTATAATCTTGTGAATTCTCCACACTTAAAACCTGCCTGGGTCTTAGACAGAGCACTTTGGCGTTT; CTCCTGTGATGTTGCAGAAGGGAAATACAAAGAAAAGGGAATACCTGCTTTGATTGAAAATGATCACCAT; ATGAATTCCATCCGAAAAATAATTTGGGAGGATATTTTTCCAAAGCTTAAACTCTGGGAATTTTTCCAAG; TAGATGTCAACAAAGCGGTTGAGCAATTTAGAAGACTTCTTACACAAGAAAATAGGCGAGTAACCAAGTC; TGATCCAAACCAACACCTTACGATTATTCAAGATCCTGAATACAGACGGTTTGGCTGTACTGTAGATATG; AACATTGCACTAACGACTTTCATACCACATGACAAGGGGCCAGCAGCAATTGAAGAATGCTGTAATTGGT; TTCATAAAAGAATGGAGGAATTAAATTCAGAGAAGCATCGACTCATTAACTATCATCAGGAACAGGCAGT; TAATTGCCTTTTGGGAAATGTGTTTTATGAACGACTGGCTGGCCATGGTCCAAAACTAGGACCTGTCACT; AGAAAGCATCCTTTAGTTACCAGGTATTTTACTTTCCCATTTGAAGAGATAGACTTCTCCATGGAAGAAT; CTATGATTCATCTGCCAAATAAAGCTTGTTTTCTGATGGCACACAATGGATGGGTAATGGGAGATGATCC; TCTTCGAAACTTTGCTGAACCGGGTTCAGAAGTTTACCTAAGGAGAGAACTTATTTGCTGGGGAGACAGT; GTTAAATTACGCTATGGGAATAAACCAGAGGACTGTCCTTATCTCTGGGCACACATGAAAAAATACACTG; AAATAACTGCAACTTATTTCCAGGGAGTACGTCTTGATAACTGCCACTCAACACCTCTTCACGTAGCTGA; GTACATGTTGGATGCTGCTAGGAATTTGCAACCCAATTTATATGTAGTAGCTGAACTGTTCACAGGAAGT; GAAGATCTGGACAATGTCTTTGTTACTAGACTGGGCATTAGTTCCTTAATAAGAGAGGCAATGAGTGCAT; ATAATAGTCATGAAGAGGGCAGATTAGTTTACCGATATGGAGGAGAACCTGTTGGATCCTTTGTTCAGCC; CTGTTTGAGGCCTTTAATGCCAGCTATTGCACATGCCCTGTTTATGGATATTACGCATGATAATGAGTGT; CCTATTGTGCATAGATCAGCGTATGATGCTCTTCCAAGTACTACAATTGTTTCTATGGCATGTTGTGCTA; GTGGAAGTACAAGAGGCTATGATGAATTAGTGCCTCATCAGATTTCAGTGGTTTCTGAAGAACGGTTTTA; CACTAAGTGGAATCCTGAAGCATTGCCTTCAAACACAGGTGAAGTTAATTTCCAAAGCGGCATTATTGCA; GCCAGGTGTGCTATCAGTAAACTTCATCAGGAGCTTGGAGCCAAGGGTTTTATTCAGGTGTATGTGGATC; AAGTTGATGAAGACATAGTGGCAGTAACAAGACACTCACCTAGCATCCATCAGTCTGTTGTGGCTGTATC; TAGAACTGCTTTCAGGAATCCCAAGACTTCATTTTACAGCAAGGAAGTGCCTCAAATGTGCATCCCTGGC; AAAATTGAAGAAGTAGTTCTTGAAGCTAGAACTATTGAGAGAAACACGAAACCTTATAGGAAGGATGAGA; ATTCAATCAATGGAACACCAGATATCACAGTAGAAATTAGAGAACATATTCAGCTTAATGAAAGTAAAAT; TGTTAAACAAGCTGGAGTTGCCACAAAAGGGCCCAATGAATATATTCAAGAAATAGAATTTGAAAACTTG; TCTCCAGGAAGTGTTATTATATTCAGAGTTAGTCTTGATCCACATGCACAAGTCGCTGTTGGAATTCTTC; GAAATCATCTGACACAATTCAGTCCTCACTTTAAATCTGGCAGCCTAGCTGTTGACAATGCAGATCCTAT; ATTAAAAATTCCTTTTGCTTCTCTTGCCTCCAGATTAACTTTGGCTGAGCTAAATCAGATCCTTTACCGA; TGTGAATCAGAAGAAAAGGAAGATGGTGGAGGGTGCTATGACATACCAAACTGGTCAGCCCTTAAATATG; CAGGTCTTCAAGGTTTAATGTCTGTATTGGCAGAAATAAGACCAAAGAATGACTTGGGGCATCCTTTTTG; TAATAATTTGAGATCTGGAGATTGGATGATTGACTATGTCAGTAACCGGCTTATTTCACGATCAGGAACT; ATTGCTGAAGTTGGTAAATGGTTGCAGGCTATGTTCTTCTACCTGAAGCAGATCCCACGTTACCTTATCC; CATGTTACTTTGATGCTATATTAATTGGTGCATATACCACTCTTCTGGATACAGCATGGAAGCAGATGTC; AAGCTTTGTTCAGAATGGTTCAACCTTTGTGAAACACCTTTCATTGGGTTCAGTTCAACTGTGTGGAGTA; GGAAAATTCCCTTCCCTGCCAATTCTTTCACCTGCCCTAATGGATGTACCTTATAGGTTAAATGAGATCA; CAAAAGAAAAGGAGCAATGTTGTGTTTCTCTAGCTGCAGGCTTACCTCATTTTTCTTCTGGTATTTTCCG; CTGCTGGGGAAGGGATACTTTTATTGCACTTAGAGGTATACTGCTGATTACTGGACGCTATGTAGAAGCC; AGGAATATTATTTTAGCATTTGCGGGTACCCTGAGGCATGGTCTCATTCCTAATCTACTGGGTGAAGGAA; TTTATGCCAGATACAATTGTCGGGATGCTGTGTGGTGGTGGCTGCAGTGTATCCAGGATTACTGTAAAAT; GGTTCCAAATGGTCTAGACATTCTCAAGTGCCCAGTTTCCAGAATGTATCCTACAGATGATTCTGCTCCT; TTGCCTGCTGGCACACTGGATCAGCCATTGTTTGAAGTCATACAGGAAGCAATGCAAAAACACATGCAGG; GCATACAGTTCCGAGAAAGGAATGCTGGTCCCCAGATAGATCGAAACATGAAGGACGAAGGTTTTAATAT; AACTGCAGGAGTTGATGAAGAAACAGGATTTGTTTATGGAGGAAATCGTTTCAATTGTGGCACATGGATG; GATAAAATGGGAGAAAGTGACAGAGCTAGAAACAGAGGAATCCCAGCCACACCAAGAGATGGGTCTGCTG; TGGAAATTGTGGGCCTGAGTAAATCTGCTGTTCGCTGGTTGCTGGAATTATCCAAAAAAAATATTTTCCC; TTATCATGAAGTCACAGTAAAAAGACATGGAAAGGCTATAAAGGTCTCATATGATGAGTGGAACAGAAAA; ATACAAGACAACTTTGAAAAGCTATTTCATGTTTCCGAAGACCCTTCAGATTTAAATGAAAAGCATCCAA; ATCTGGTTCACAAACGTGGCATATACAAAGATAGTTATGGAGCTTCAAGTCCTTGGTGTGACTATCAGCT; CAGGCCTAATTTTACCATAGCAATGGTTGTGGCCCCTGAGCTCTTTACTACAGAAAAAGCATGGAAAGCT; TTGGAGATTGCAGAAAAAAAATTGCTTGGTCCCCTTGGCATGAAAACTTTAGATCCAGATGATATGGTTT; ACTGTGGAATTTATGACAATGCATTAGACAATGACAACTACAATCTTGCTAAAGGTTTCAATTATCACCA; AGGACCTGAGTGGCTGTGGCCTATTGGGTATTTTCTTCGTGCAAAATTATATTTTTCCAGATTGATGGGC; CCGGAGACTACTGCAAAGACTATAGTTTTGGTTAAAAATGTTCTTTCCCGACATTATGTTCATCTTGAGA; GATCCCCTTGGAAAGGACTTCCAGAACTGACCAATGAGAATGCCCAGTACTGTCCTTTCAGCTGTGAAAC; ACAAGCCTGGTCAATTGCTACTATTCTTGAGACACTTTATGATTTATAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000028 unedited; GCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGAGGCCGCGCCTTGG; CCGGTGCACCTTCCCCAGGCAAGGAGAAAGCGCCGCTCCCGGCCTCAGCCGCAGCAGGCT; CCAGGTCCCCCGGCCCGGAGCCGACTGAGACGGTGCGGTGCCCACGCTCTCGCGAGACTA; GCGGTCGGGGCGGGCGGGTCGAGCCTCCCGGAAGTGGGCCAGAGGGGTAACTCATTCGAC; TGTGGAGTTCTTTTAATTCTTATGAAAGATTTCAAATCCTCTAGAAGCCAAAATGGGACA; CAGTAAACAGATTCGAATTTTACTTCTGAACGAAATGGAGAAACTGGAAAAGACCCTCTT; CAGACTTGAACAAGGGTATGAGCTACAGTTCCGATTAGGCCCAACTTTACAGGGAAAAGC; AGTTACCGTGTATACAAATTACCCATTTCCTGGAGAAACATTTAATAGAGAAAAATTCCG; TTCTCTGGATTGGGAAAATCCAACAGAAAGAGAAGATGATTCTGATAAATACTGTAAACT; TAATCTGCAACAATCTGGTTCATTTCAGTATTATTTCCTTCAAGGAAATGAGAAAAGTGG; TGGAGGTTACATAGTTGTGGACCCCNATTTTACGTGTGGTGCTGATAATCATGTGCTACC; CTTGGACTGTGTTACTCTTCAGACTTNNTAGCCTAGTGTTTGGGACCTTTTGATGAATGG; GAAAGCAGACTTAAGGTTGCAAAGAATCAGGCTACACATGATTCATTNTACCCCATTGCA; GACTCTTGGACTATCTAGGTCATGCTACTCCCTTGNCNATCAGTTAGAATTAATTCTGAC; TTTTTCAGACCTATANAAAGTTACCTGGAATGGATGTTTGAACAGCTAGTGGAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000028 unedited; TAGCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGACCTTT; AGAAAATATTTTTATTTTCTTAACACACAGAGGTAACAATGACATTTGCCCACTAGACAC; AGTAGTTCAAGACATATCAGAAATGGATCACAATTCACATGTTACAATATTCATCAATAT; GACCAGTCTCATGTTTTCCCAATTATCTTTGAAAACTTATTAAAATACCACAGCATACTA; AACTGGTAAATTCATTTATCATGTTAAAGAGTAATTTTGATTTTGATTAGTGAAAAACCA; AGGCATAGTCCATTGTTGAAATACAATACCTTATCCAGCATCACTCAAGTATGAAGCATT; CTACCGAAACGTTTAGTCAAATGACACCTGTTAAAAATCTAATTCTGTATTTTAACTACC; ATAGAGGAAATAATGTTTAACATGCCTTCTTAAACTTTAAAATATACCACGCAGAATATA; ACAATGTTCTTTCATTAATCTTCCTCATTAGAAATATGAACCACTTAGTTTTCTAGTGCA; ACACAATATCCTAAAAATTTACCAACGTGGGTATCTGCCTTACTAGAGATAACCCCAAAT; GGCTACATTAAAAAAATTGTGAACTTTTAATTTTTTTGGTCACATTTAACTGTCAGCGAT; TACTTCTTGCTTTGATTAAATATGCTGGGTTTCTGTTACCTTTCCTACCTCCCCTGATAT; CATAGTTAGGGTACAGCACCAAAACTACAAAACCTTTGATAAACCAATGGTTCTTTTAAA; GGCAGTCATTTCTTTTACCCGCCAGTTTAAAGAAGGGATTTTTTGACCAGGAAGGAACCT; GAGAACAAAGAAGATTTGTAAACCATGGCCATGTTTTATAGATGTGGTCAAATAACGGCC; AACTTGCCTTAATTGGGAGAATTAACCATTTTTATTTTGAAACACAAACTATCTTGTTAA; N;
• Restriction Sites: NotI-NotI
• ACCN: NM_000028
• Insert Size: 7070 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000028.1, NP_000019.1
• RefSeq Size: 7445 bp
• RefSeq ORF: 4599 bp
• Locus ID: 178
• UniProt ID: P35573
• Cytogenetics: 1p21.2
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism
• Summary: This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (1). Variants 1, 2, 3 and 4 encode the same isoform (isoform 1).