NDUFS5 (NM_004552) Human Untagged Clone

SKU SC117273

NDUFS5 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase) (NDUFS5), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NDUFS5
• Synonyms: CI-15k; CI15K
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004552 edited; ATGCCTTTCTTGGACATCCAGAAAAGGTTCGGCCTTAACATAGATCGATGGTTGACAATC; CAGAGTGGTGAACAGCCCTACAAGATGGCTGGTCGATGCCATGCTTTTGAAAAAGAATGG; ATAGAATGTGCACATGGAATCGGTTATACTCGGGCAGAGAAAGAGTGCAAGATAGAATAT; GATGATTTCGTAGAGTGTTTGCTTCGGCAGAAAACGATGAGACGTGCAGGTACCATCAGG; AAGCAGCGGGATAAGCTGATAAAGGAAGGAAAGTACACCCCTCCACCTCACCACATTGGC; AAGGGGGAGCCTCGGCCCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004552 unedited; TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGAGCATCGGCCATGCCTTTC; TTGGACATCCAGAAAAGTTCGGCCTTAACATAGATCGATGGTTGACAATCCAGAGTGGTG; AACAGCCCTACAAGATGGCTGGTCGATGCCATGCTTTTGAAAAAGAATGGATAGAATGTG; CACATGGAATCGGTTATACTCGGGCAGAGAAAGAGTGCAAGATAGAATATGATGATTTCG; TAGAGTGTTTGCTTCGGCAGAAAACGATGAGACGTGCAGGTACCATCAGGAAGCAGCGGG; ATAAGCTGATAAAGGAAGGAAAGTACACCCCTCCACCTCACCACATTGGCAAGGGGGAGC; CTCGGCCCTGAACAGAGCAGCTGCTGATGTCTGGAGGCTGATTTTCCTGTTCTCTGTTCT; CCACTGGAAAGGTTGTTTACGACAAACCTCCTTGTCAAAGCTGGAGTGCGATGGCGTGGT; CTCAGCTTACCGCAACCTCCGCCTCCCGGGTTGAAGCGATTCTCCTGCCTCAGCTGCTGA; GTAGCTGGGATTACAGGTGTGGCATCTGGCATGAAACCAAGGACAAGACACTCAGTTGAG; CTTTGTGAAAGAGTGACAGATGATGGATAGAGTCATGGGCCTGCCTCTTATGGAGCTAAG; AAATGAAATCTGGATACGGGTAATCAGTTTGTGGTAGAAAGTAGTCACTCATTTCCGTGA; GGGAAGCACAAGTCCTGTGTANGCCCAGAGAAAGGAAAAAGCCTTTNNTGGCATAANAGT; GAAATGTGAGGGGTTGAGAGAGAAATGTCTGNANNGCTTGTANCATTCATGTTGTGCCCC; TAAGGGACAGATACATTTGGACATGAGAAGCTGTAGTGAGCCATGACTGCACACTGCACT; TCCAGCTGGGCGACAGGTTGAGACCCTGNNNCTCAAAAANANANNANNNNNNAGAANNAN; NAAAAAAACTCGCTCTAATG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004552 unedited; CGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTA; GAAAAGGGTCTCAACCTGTCGCCCAACCTGGAGTGCAGTGGTGCAGTCATGGCTCACTAC; AGCTTCTCATGTCCAAATGTTATCTGTCCTTTAGGGCACAACATGAATGCTACAAAGCCT; CCAAACCATTCCTCTCTCCAACCCTCAACATTCACTCTTATGCCCAAAAAGGCTTTTTCC; CTTCTCTGGGCCTACACAAGACTTGTGCTTCCCTCACGGAAATGAGTGACTACTTTCTAC; CACAAACTGATTACCCGTATCCAAATTTCATTTCTTAACTCCATAAAAAGCAGGCCCATG; ACTCTATCCATCATCTGTCACTCTTTCACAAAGCTCAACTGAGTGTCTTGGCCTTGGTTT; CATGCCAGATGCCACACCTGTAATCCCAGCTACTCAGCAGCTGAGGCAGGAGAATCGCTT; CAACCCGGGAGGCGGAGGTTGCGGTAAGCTGAAACCACGCCATCGCACTCCAGCTTTGAC; AAGGAAGTTTGTCGTAAACAACCTTTCCAGTGGAGAACAGAGAACAGGAAAATCAGCCTC; CAGACATCANCAGCTGCTCTGTTCAAGGCCGAAGCCTCCCCCTTGCCAATGTGGTGAGGT; GGAAGGGTGTACTTTCCTTCCTTTATCAGCTTATCCCGCTGCTTCCTGATGGTACCTGCA; CGTCTCATACGTTTCCTGCCGAGCAAACACTCTACGAAATCATCATATTCTATCTTGCAC; TCTTTCTTCTGCCGAGTTTAACCGATTCCATGTGCACATCTATTCATTCTTTTTAAAAGC; ATGGATCGACCAGCCATTTGTAAGGCTTGTCACACTCTTGATTGTAACCCTCGATCTATT; TAAGGCCAAACTTTTTGGATGCCANAAAGCATGCCGATCTCNCCTCTGCCGATTTCGGCC; GCCCTTATAAGTCGTTTACAAATTTGAGGTCATAATCAACCTGCTATTAGACCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_004552
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004552.1, NP_004543.1
• RefSeq Size: 540 bp
• RefSeq ORF: 321 bp
• Locus ID: 4725
• UniProt ID: O43920
• Cytogenetics: 1p34.3
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Summary: This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17. [provided by RefSeq, May 2010]; Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.