Cyclin B2 (CCNB2) (NM_004701) Human Untagged Clone

SKU SC117206

CCNB2 (untagged)-Human cyclin B2 (CCNB2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cyclin B2
• Synonyms: HsT17299
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004701, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGCTGCTCCGACGCCCGACGGTGTCCAGTGATTTGGAGAATATTGACACAGGAGTTAATTCTAAAG; TTAAGAGTCATGTGACTATTAGGCGAACTGTTTTAGAAGAAATTGGAAATAGAGTTACAACCAGAGCAGC; ACAAGTAGCTAAGAAAGCTCAGAACACCAAAGTTCCAGTTCAACCCACCAAAACAACAAATGTCAACAAA; CAACTGAAACCTACTGCTTCTGTCAAACCAGTACAGATGGAAAAGTTGGCTCCAAAGGGTCCTTCTCCCA; CACCTGAGGATGTCTCCATGAAGGAAGAGAATCTCTGCCAAGCTTTTTCTGATGCCTTGCTCTGCAAAAT; CGAGGACATTGATAACGAAGATTGGGAGAACCCTCAGCTCTGCAGTGACTACGTTAAGGATATCTATCAG; TATCTCAGGCAGCTGGAGGTTTTGCAGTCCATAAACCCACATTTCTTAGATGGAAGAGATATAAATGGAC; GCATGCGTGCCATCCTAGTGGATTGGCTGGTACAAGTCCACTCCAAGTTTAGGCTTCTGCAGGAGACTCT; GTACATGTGCGTTGGCATTATGGATCGATTTTTACAGGTTCAGCCAGTTTCCCGGAAGAAGCTTCAATTA; GTTGGGATTACTGCTCTGCTCTTGGCTTCCAAGTATGAGGAGATGTTTTCTCCAAATATTGAAGACTTTG; TTTACATCACAGACAATGCTTATACCAGTTCCCAAATCCGAGAAATGGAAACTCTAATTTTGAAAGAATT; GAAATTTGAGTTGGGTCGACCCTTGCCACTACACTTCTTAAGGCGAGCATCAAAAGCCGGGGAGGTTGAT; GTTGAACAGCACACTTTAGCCAAGTATTTGATGGAGCTGACTCTCATCGACTATGATATGGTGCATTATC; ATCCTTCTAAGGTAGCAGCAGCTGCTTCCTGCTTGTCTCAGAAGGTTCTAGGACAAGGAAAATGGAACTT; AAAGCAGCAGTATTACACAGGATACACAGAGAATGAAGTATTGGAAGTCATGCAGCACATGGCCAAGAAT; GTGGTGAAAGTAAATGAAAACTTAACTAAATTCATCGCCATCAAGAATAAGTATGCAAGCAGCAAACTCC; TGAAGATCAGCATGATCCCTCAGCTGAACTCAAAAGCCGTCAAAGACCTTGCCTCCCCACTGATAGGAAG; GTCCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004701 unedited; NGGTGCAAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGTAC; GCTAGTGTCCTCCCTTTTCAGTCCGCGTCCCTCCCTGGGCCGGGCTGGCACTCTTGCCTT; CCCCGTCCCTCATGGCGCTGCTCCGACGCCCGACGGTGTCCAGTGATTTGGAGAATATTG; ACACAGGAGTTAATTCTAAAGTTAAGAGTCATGTGACTATTAGGCGAACTGTTTTAGAAG; AAATTGGAAATAGAGTTACAACCAGAGCAGCACAAGTAGCTAAGAAAGCTCAGAACACCA; AAGTTCCAGTTCAACCCACCAAAACAACAAATGTCAACAAACAACTGAAACCTACTGCTT; CTGTCAAACCAGTACAGATGGAAAAGTTGGCTCCAAAGGGTCCTTCTCCCACACCTGAGG; ATGTCTCCATGAAGGAAGAGAATCTCTGCCAAGCTTTTTCTGATGCCTTGCTCTGCAAAA; TCGAGGACATTGATAACGAAGATTGGGAGAACCCTCAGCTCTGCAGTGACTACGTTAAGG; ATATCTATCAGTATCTCAGGCAGCTGGAGGTTTTGCAGTCCATAAACCCACATTTCTTAG; ATGGAAGAGATATAAATGGACGCATGCGTGCCATCCTAGTGGATTGGCTGGTACAAGTCC; ACTCCAAGTTTAGGCTTCTGCAGGAGACTCTGTACATGTGCGTTGGCATTATGGATCGAT; TTTTACAGGTTCAGCCAGTTTCCCGGAAGAAGCTTNCATTAGTTGGGATTACTGCTCTGC; TCTTGGCTTCCAGTATGAGGAGATGTTTTCTCCAATATTTGAGACTTGTTACATCACAGA; CATGCTTATACAGNTNCCAATCCGAGAATGGAACTCTAATTTGAAAGAATGAAATTGGAN; TGGGTCGACCTTGCACTACCTTCTTAGGCGAGCTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004701 unedited; CCTCTATGGACCGCGGCCCGCTATCTATGATCGGTTTTTTTTTTTTTTTTTTACCTTAAA; AAAAACAATCGCTTTATTTTTCCTCATATATGTTTAGAAAACTGGTCTGAGAAGAGGTTT; CATGAGATAGACCAGAGGACTATGTACAAAATCAAGAGTTCTAAACCAATAAGAAAAAGG; GCACAATGAAGCACACATCCCCAGGGGCCACGGCAGCCTAGGACCTTCCTATCAGTGGGG; AGGCAAGGTCTTTGACGGCTTTTGAGTTCAGCTGAGGGATCATGCTGATCTTCAGGAGTT; TGCTGCTTGCATACTTATTCTTGATGGCCTGCAGAAGTCATTTGTGATTAAGCACATGTC; AGGAACCCCCAATGATACCTTATTCCTAGTCTAACTCACAATTATCAAAGGTCCACGGCT; ACAATCATGATCACATTGATGATGCCCAATCGCTGAAGCCAGTGAAAGCTCTGGGCTCAT; AACATGTGCCTGACCATTTCCTTCCATCCCCATTAATGAATAAAACACAGCTGGTAGCAC; AGGTGATAGGAACAAATGATTTCAAGGTCCAAGTAGCAGGCTTCCTCTTGTAGGCTACCA; GAGGGATGGGAGGGGGAGCCTGTGTTTACGACACCTAAGTGCCATCAGGACATAACAGGT; TCACCAGGCCTGGCAGCAATAGGAACAATTAAAAGACACTAAAAGACACTAAAAGAGGGT; GGCAACTAGTGCTTAACAAATCCCAGACAGTCCTCTCAGGACTGAACACATATTACACTT; CCATGAAGCAAACATGTTGTGAGGACACTTGAGACTCTTTTGAAGTTCATATACCTTGCT; CTTTGCCCCCACACCCTCGGGAGCTCTGGATGTTGGGCGCTCACAAGCCCAGGACAGCAC; TAATTTCCCTCGGCTTCCCCTAAAGAGGGCCTGGCCGAATAGAACCCTGCCCTTAGGATG; CCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_004701
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004701.2, NP_004692.1
• RefSeq Size: 1530 bp
• RefSeq ORF: 1197 bp
• Locus ID: 9133
• UniProt ID: O95067
• Cytogenetics: 15q22.2
• Domains: cyclin, CYCLIN, cyclin_C
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation
• Summary: Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]