LRRFIP1 (NM_004735) Human Untagged Clone

SKU SC117183

LRRFIP1 (untagged)-Human leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: LRRFIP1
• Synonyms: FLAP-1; FLAP1; FLIIAP1; GCF-2; GCF2; HUFI-1; TRIP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117183 sequence for NM_004735 edited (data generated by NextGen Sequencing); ATGACCAGCCCCGCGGCCGCTCAAAGCCGGGAGATCGACTGTTTGAGCCCGGAAGCGCAG; AAGCTGGCGGAAGCCCGGCTCGCTGCAAAACGGGCGGCCCGCGCGGAGGCTCGCGAGATC; CGCATGAAGGAGCTGGAGCGGCAGCAGAAGGAGGAAGACAGTGAGCGCTACTCTCGTAGA; TCCAGAAGAAACACATCGGCTTCTGATGAAGACGAGCGCATGTCAGTGGGTAGTCGTGGA; AGCCTGAGGGTAGAAGAGAGACCAGAAAAAGATTTTACTGAGAAGGGGTCTCGTAACATG; CCGGGCCTGTCTGCAGCCACGCTGGCCTCTCTGGGTGGGACTTCCTCTCGGAGAGGCAGC; GGAGACACCTCCATCTCCATCGACACCGAGGCATCCATCAGGGAAATCAAGGACTCTCTA; GCAGAAGTTGAAGAGAAATATAAGAAGGCTATGGTTTCCAATGCTCAGCTAGACAATGAA; AAGACAAACTTCATGTACCAGGTTGATACCCTAAAAGATATGTTGCTGGAGCTTGAAGAA; CAGCTGGCTGAATCTAGGCGGCAGTACGAAGAGAAAAACAAAGAATTTGAAAGGGAAAAA; CACGCCCACAGTATACTGCAATTTCAGTTTGCTGAAGTCAAGGAGGCCCTGAAGCAAAGA; GAGGAAATGCTCGAGAAACATGGAATAATCCTAAATTCAGAAATAGCTACCAATGGAGAG; ACTTCCGACACCCTCAATAATGTTGGATACCAAGGTCCTACCAAGATGACAAAAGAAGAG; TTAAATGCCCTCAAGTCGACAGGGGATGGGACCCTAGGAAGAGCCAGTGAAGTGGAGGTG; AAAAATGAAATCGTGGCGAATGTGGGGAAAAGAGAAATCTTGCACAATACTGAGAAAGAA; CAACACACAGAGGACACAGTGAAGGACTGTGTGGACATAGAGGTATTCCCTGCTGGTGAG; AATACCGAGGACCAGAAATCCTCTGAAGACACTGCCCCATTCCTAGGAACCTTAGCAGGT; GCTACCTATGAGGAACAGGTTCAAAGCCAAATTCTTGAGAGCAGTTCTCTCCCTGAAAAC; ACAGTACAGGTTGAGTCAAATGAGGTCATGGGTGCACCAGATGACAGGACCAGAACTCCC; CTTGAGCCATCCAACTGTTGGAGTGACTTAGATGGTGGGAACCACACAGAGAATGTGGGA; GAGGCAGCAGTGACTCAGGTTGAAGAGCAGGCAGGCACAGTGGCCTCGTGTCCTTTAGGG; CATAGTGATGACACAGTTTATCATGATGACAAATGTATGGTAGAGGTCCCCCAAGAGTTA; GAGACAAGCACAGGGCATAGTTTAGAGAAAGAATTCACCAACCAGGAAGCAGCTGAGCCC; AAGGAGGTTCCAGCGCACAGTACAGAAGTAGGTAGGGATCACAACGAAGAAGAGGGTGAA; GAAACAGGATTAAGGGACGAGAAACCAATCAAGACAGAAGTTCCTGGTTCTCCAGCAGGA; ACTGAGGGCAACTGTCAGGAAGCGACAGGTCCAAGTACAGTAGACACTCAAAATGAACCC; TTAGATATGAAAGAGCCCGATGAAGAAAAGAGTGACCAACAGGGAGAGGCATTGGACTCA; TCGCAGAAGAAGACAAAGAACAAGAAAAAGAAAAACAAGAAGAAAAAATCCCCAGTACCC; GTAGAAACCCTTAAAGATGTTAAAAAAGAGTTAACGTATCAGAACACAGATTTAAGTGAA; ATTAAGGAAGAAGAGCAGGTAAAGTCTACTGACAGAAAGTCAGCAGTGGAAGCCCAAAAC; GAGGTGACTGAAAATCCAAAACAGAAAATTGCAGCAGAAAGCAGTGAAAATGTTGATTGT; CCGGAGAATCCTAAAATTAAGTTGGATGGAAAACTTGACCAAGAAGGTGATGATGTACAA; ACAGCAGCTGAGGAGGTACTAGCTGATGGAGACACATTAGATTTTGAGGATGACACCGTT; CAATCATCAGGCCCGAGGGCTGGTGGTGAAGAATTAGATGAAGGTGTTGCAAAAGATAAT; GCTAAAATAGATGGTGCCACTCAAAGCAGTCCTGCAGAACCAAAGAGCGAAGACGCAGAT; CGCTGCACCCTGCCCGAACATGAAAGTCCCTCACAGGACATTAGTGATGCCTGTGAAGCA; GAAAGTACAGAGAGGTGTGAGATGTCAGAACATCCAAGTCAGACCGTCAGGAAAGCTTTA; GACAGCAATAGCCTAGAGAACGATGACTTGTCGGCACCAGGAAGAGAGCCAGGGCACTTC; AATCCAGAAAGCAGAGAAGATACCAGAGGAGGGAATGAGAAGGGCAAAAGCAAAGAAGAC; TGTACCATGTCCTAA; ; Clone variation with respect to NM_004735.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004735 unedited; GGTTGGATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCCGAGCCCGA; CTCAGCCCGCGGCCACCTGCGCCCCGCCCCTGTCGGCCGCGCCCGAGCCCAGCGCCGCGA; GCCGCTCCCCGGCGGGCTGGCTCCTGGCCCCGGAAGCGCGAGCGTTCACTTAGCGGCGAG; TGGCTCCGTCTCCGCGGACAGAGCGCGCGCCCCCTGGCCCGGCCCGCGAGGGGCTCCCGG; CGCGGTCCCCGAGCATTTCCCGCCGGGTGGAGCGGGCCGAGCCCGGCAGGATGACCAGCC; CCGCGGCCGCTCAAAGCCGGGAGATCGACTGTTTGAGCCCGGAAGCGCAGAAGCTGGCGG; AAGCCCGGCTCGCTGCAAAACGGGCGGCCCGCGCGGAGGCTCGCGAGATCCGCATGAAGG; AGCTGGAGCGGCAGCAGAAGGAGGAAGACAGTGAGCGCTACTCTCGTAGATCCAGAAGAA; ACACATCCGGCTTCTGATGAAGACGAGCGCATGTCAGTGGGCAGTCGTGGAAGCCTGATG; GTAGAAGAGAGACCAGAAAAAGATTTTACTGAGAAGGGGTCTCGTAACATGCCGGGCCTG; TCTGCAGCCACCGCTGGCCTCTCTGGGTGGGACTTCCTCTCGGAGAGGCAGCGGAGACAC; CTCCATCTCCATCGACACCGAGGCATTCATCAGGGAAATCAAAGACTCTCTACCAGAATT; TGAAGAGAAATACTACAAGGCTATGGTTTCCCATGCTCAGCTATACCACGAAAAGACAAA; CTTCATGGTCCCAGGGTGATACGCTCAAAGATATGTTGCCTGAGCTTTGAAGAACAGCTG; GCTGAATCTCAGCGGCGTTCCGAGCAAGAAAACCACGCAATTGCCAAGGGAGAAACATCC; CCAACAGTATACTGCATTTTCATGTCTGCTGAAGCTCACG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004735 unedited; GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGAGACAGTGTCTCACTCTGTCTCCC; AGGCTAGAGTACAGTGTGCAATCATAGCTCACTGCAGCCTTGAACTCCTGGCCTCAAGCA; ATCCTCCCACCTTGGCTTCCCAATGTGCTAGGTTTACAGGCGTGAAAACATTGTGTTGTG; TGTGTGTGTGTTTTTTTTTTTTTTTTTAAACAGAGTTTCGCTCTTGTTGTCCAGGCTGGA; GTACAATGGCTTGATCTTGGCTCACTGCAATCTCTGCCTCGCAGGTTCAAGCGATTCTCC; TGCCTCAGCCTCTGGAGTGGCTGGGATTACAGGCGCCCACCATCACGCCCGGCCAATTTT; TTGTATTTTTAGTAAAGACAGGGTTTCACCATGTTGGCCAGGCTGGCCTCGAACTCTTGA; CCTCAGGTGATCCACCTGCCTTGGCCTCCCAATGTACTGAGATTACAGGCATGAGCCACC; ACGCCCGGCCAAAAACATTTAAAAAATGACTGTCCCTGCTCAAATACTGCAGTAGGAAAT; GTAATTTGACATATATCACTTCCAGAAAAAAACTTTAAATCTTTCTATAAAATGAATTTG; ATACATCATCAGCATGAAGTGAAGTAAAATCTCTTACAAGTAATTCAGGTATATAACATG; AGATCCAAAGTATCGGTTCAAGACAAAGACTCTATGAGGTAAGGCAAATAAGCCCCTATG; CTTTAGACATTACTTGTATCCCTAAAGATATTGTTCAAGCGCAAATTTATATTCAAGACC; TATTGCTTTGGCTGTTATCTCCTTAATAAATACCTACCGGTATATGCACTTTTTAATCAA; TTGAAGGCCCCTTGGGACAGAACTAATTAATTCAATTTTCCTAAACCAAGTCAAAAGCCT; TTGGCTTCATTAAAGCCGTTTTTGGCAAAAACCCTTCTTTACCAAGAAATAAAGCCCTTT; ATAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004735
• Insert Size: 4260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004735.2, NP_004726.1
• RefSeq Size: 3648 bp
• RefSeq ORF: 2355 bp
• Locus ID: 9208
• UniProt ID: Q32MZ4
• Cytogenetics: 2q37.3
• Protein Families: Transcription Factors
• Summary: Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (4) lacks an in-frame exon in the coding region, compared to variant 3. The resulting isoform (4) lacks an internal segment, compared to isoform 3.