TRIM25 (NM_005082) Human Untagged Clone

SKU SC116977

TRIM25 (untagged)-Human tripartite motif containing 25 (TRIM25)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRIM25
• Synonyms: EFP; RNF147; Z147; ZNF147
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005082, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCAGAGCTGTGCCCCCTGGCCGAGGAGCTGTCGTGCTCCATCTGCCTGGAGCCCTTCAAGGAGCCGG; TCACCACTCCGTGCGGCCACAACTTCTGCGGGTCGTGCCTGAATGAGACGTGGGCAGTCCAGGGCTCGCC; ATACCTGTGCCCGCAGTGCCGCGCCGTCTACCAGGCGCGACCGCAGCTGCACAAGAACACGGTGCTGTGC; AACGTGGTGGAGCAGTTCCTGCAGGCCGACCTGGCCCGGGAGCCACCCGCCGACGTCTGGACGCCGCCCG; CCCGCGCCTCTGCACCCAGCCCGAATGCCCAGGTGGCCTGCGACCACTGCCTGAAGGAGGCCGCCGTGAA; GACGTGCTTGGTGTGCATGGCCTCCTTCTGTCAGGAGCACCTGCAGCCGCACTTCGACAGCCCCGCCTTC; CAGGACCACCCGCTGCAGCCGCCCGTTCGCGACCTGTTGCGCCGCAAATGTTCCCAGCACAATCGGCTGC; GGGAATTTTTCTGCCCCGAGCACAGCGAGTGCATCTGCCACATCTGCCTGGTGGAGCATAAGACCTGCTC; TCCCGCGTCCCTGAGCCAGGCCAGCGCCGACCTGGAGGCCACCCTGAGGCACAAACTAACTGTCATGTAC; AGTCAGATCAACGGGGCGTCGAGAGCACTGGATGATGTGAGAAACAGGCAGCAGGATGTGCGGATGACTG; CAAACAGAAAGGTGGAGCAGCTACAACAAGAATACACGGAAATGAAGGCTCTCTTGGACGCCTCAGAGAC; CACCTCGACAAGGAAGATAAAGGAAGAGGAGAAGAGGGTCAACAGCAAGTTTGACACCATTTATCAGATT; CTCCTCAAGAAGAAGAGTGAGATCCAGACCTTGAAGGAGGAGATTGAACAGAGCCTGACCAAGAGGGATG; AGTTCGAGTTTCTGGAGAAAGCATCAAAACTGCGAGGAATCTCAACAAAGCCAGTCTACATCCCCGAGGT; GGAACTGAACCACAAGCTGATAAAAGGCATCCACCAGAGCACCATAGACCTCAAAAACGAGCTGAAGCAG; TGCATCGGGCGGCTCCAGGAGCCCACCCCCAGTTCAGGTGACCCTGGAGAGCATGACCCAGCGTCCACAC; ACAAATCCACACGCCCTGTGAAGAAGGTCTCCAAAGAGGAAAAGAAATCCAAGAAACCTCCCCCTGTCCC; TGCCTTACCCAGCAAGCTTCCCACGTTTGGAGCCCCGGAACAGTTAGTGGATTTAAAACAAGCTGGCTTG; GAGGCTGCAGCCAAAGCCACCAGCTCACATCCGAACTCAACATCTCTCAAGGCCAAGGTGCTGGAGACCT; TCCTGGCCAAGTCCAGACCTGAGCTCCTGGAGTATTACATTAAAGTCATCCTGGACTACAACACCGCCCA; CAACAAAGTGGCTCTGTCAGAGTGCTATACAGTAGCTTCTGTGGCTGAGATGCCTCAGAACTACCGGCCG; CATCCCCAGAGGTTCACATACTGCTCTCAGGTGCTGGGCCTGCACTGCTACAAGAAGGGGATCCACTACT; GGGAGGTGGAGCTGCAGAAGAACAACTTCTGTGGGGTAGGCATCTGCTACGGAAGCATGAACCGGCAGGG; CCCAGAAAGCAGGCTCGGCCGCAACAGCGCCTCCTGGTGCGTGGAGTGGTTCAACACCAAGATCTCTGCC; TGGCACAATAACGTGGAGAAAACCCTGCCCTCCACCAAGGCCACGCGGGTGGGCGTGCTTCTCAACTGTG; ACCACGGCTTTGTCATCTTCTTCGCTGTTGCCGACAAGGTCCACCTGATGTATAAGTTCAGGGTGGACTT; TACTGAGGCTTTGTACCCGGCTTTCTGGGTATTTTCTGCTGGTGCCACACTCTCCATCTGCTCCCCCAAG; TAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005082 unedited; GTCATAACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT; AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC; CGCGAATTCGGCACGAGGCGCGGGTGCAGCAGTTGTGTCCCGACCCCTGGGAGCGCCATG; GCAGAGCTGTGCCCCCTGGCCGAGGAGCTGTCGTGCTCCATCTGCCTGGAGCCCTTCAAG; GAGCCGGTCACCACTCCGTGCGGCCACAACTTCTGCGGGTCGTGCCTGAATGAGACGTGG; GCAGTCCAGGGCTCGCCATACCTGTGCCCGCAGTGCCGCGCCGTCTACCAGGCGCGACCG; CAGCTGCACAAGAACACGGTGCTGTGCAACGTGGTGGAGCAGTTCCTGCAGGCCGACCTG; GCCCGGGAGCCACCCGCCGACGTCTGGACGCCGCCCGCCCGCGCCTCTGCACCCAGCCCG; AATGCCCAGGTGGCCTGCGACCACTGCCTGAAGGAGGCCGCCGTGAAGACGTGCTTGGTG; TGCATGGCCTCCTTCTGTCAGGAGCACCTGCAGCCGCACTTCGACAGCCCCGCCTTCCAG; GACCACCCGCTGCAGCCGCCCGTTCGCGACCTGTTGCGCCGCANATGTTCCCAGCACAAT; CGGCTGCGGGAATTTTTCTGCCCCGAGCACAGCGAGTGCATCTGCCACATCTGCCTGGTG; GAGCATAAGACCTGCTCTNCCGCGTCCCTGAGCCAGGCCAGCGCCGACCTGNNAGCCACC; CTTGAGCACANACTAACTGTCATGTACAGTCAGATCAACGGNGCGTCGAGAGCACTGGAT; GATGTGAGAAACAGGGCAGCAGATGTGCGGATGACTGCNAACAGAAGGTGGAGCAGCTAC; CNACAGATACACGGAATGAAGGCTCTCTTGTACGCTCAGAGACC;
• Restriction Sites: NotI-NotI
• ACCN: NM_005082
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005082.4, NP_005073.2
• RefSeq Size: 5744 bp
• RefSeq ORF: 1893 bp
• Locus ID: 7706
• UniProt ID: Q14258
• Cytogenetics: 17q22
• Domains: PRY, RING, SPRY
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: RIG-I-like receptor signaling pathway
• Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]