BMI1 (NM_005180) Human Untagged Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | BMI1 |
| Synonyms | flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_005180 |
| Insert Size | 2700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_005180.5. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005180.5, NP_005171.4 |
| RefSeq Size | 3251 bp |
| RefSeq ORF | 981 bp |
| Locus ID | 648 |
| UniProt ID | P35226 |
| Cytogenetics | 10p12.2 |
| Domains | RING |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
| Summary | This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005180" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202871 | BMI1 (Myc-DDK-tagged)-Human BMI1 polycomb ring finger oncogene (BMI1) | 10 ug |
$450.00
|
|
| RC202871L1 | Lenti ORF clone of Human BMI1 polycomb ring finger oncogene (BMI1), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202871L2 | Lenti ORF clone of Human BMI1 polycomb ring finger oncogene (BMI1), mGFP tagged | 10 ug |
$750.00
|
|
| RC202871L3 | Lenti ORF clone of Human BMI1 polycomb ring finger oncogene (BMI1), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202871L4 | Lenti ORF clone of Human BMI1 polycomb ring finger oncogene (BMI1), mGFP tagged | 10 ug |
$750.00
|
|
| RG202871 | BMI1 (tGFP-tagged) - Human BMI1 polycomb ring finger oncogene (BMI1) | 10 ug |
$650.00
|
|
Citations
Bmi1 essentially mediates podocalyxin-enhanced Cisplatin chemoresistance in oral tongue squamous cell carcinoma
Zhou, Zhang, Pan et al
PLoS ONE (2015) 10 (4), e0123208 DOI: 10.1371/journal.pone.0123208
Zhou, Zhang, Pan et al
PLoS ONE (2015) 10 (4), e0123208 DOI: 10.1371/journal.pone.0123208
Cited by 30
AI Summary: OriGene contributed to this study by providing the human Bmi1 cDNA clone (catalog number SC116894), which was used to investigate the role of Bmi1 in podocalyxin-enhanced cisplatin chemoresistance in oral tongue squamous cell carcinoma cells.
Snippet: Human full length PODXL cDNA was subcloned into the pcDNA 3.1 plasmid. Human Bmi1 cDNA clone (SC116894) was purchased from Origene (Beijing, China) and the full length Bmi1 cDNA sequence was subcloned into the pcDNA 3.1 plasmid. ... Cite SaveSaving
Polycomb group proteins as epigenetic mediators of neuroprotection in ischemic tolerance
Stapels, Piper, Yang et al
Sci Signal (2010) 3 (111), ra15 DOI: 10.1126/scisignal.2000502
Stapels, Piper, Yang et al
Sci Signal (2010) 3 (111), ra15 DOI: 10.1126/scisignal.2000502
Cited by 100
AI Summary: OriGene products, specifically the human BMI1 (RC207307) and SCMH1 (RC217771) expression plasmids, were used to overexpress PcG proteins in vitro, demonstrating that increased levels of these proteins can induce ischemic tolerance and neuroprotection in neuronal cells.
Showing 1-2 of 2 papers.
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