EWSR1 (NM_005243) Human Untagged Clone

SKU SC116868

EWSR1 (untagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: EWSR1
• Synonyms: bK984G1.4; EWS; EWS-FLI1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005243, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAGTGCTTACACCGCCC; AGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCC; CACTGATGTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTAT; GGACAGCCTCCCACTGGTTATACTACTCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATG; GCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGC; ATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACCGCAG; GATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAG; GATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAGTCACTGCACC; TCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACTAGTTATGATCAGAGCAGTTACTCTCAG; CAGAACACCTATGGGCAACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGC; AGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCCAAGTCAATATAGCCAACA; GAGCAGCAGCTACGGGCAGCAGAGTTCATTCCGACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAG; GAGTCTGGAGGATTTTCCGGACCAGGAGAGAACCGGAGCATGAGTGGCCCTGATAACCGGGGCAGGGGAA; GAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGGGCGGGGAGGAGGACGCGGTGGAATGGGCAGCGC; TGGAGAGCGAGGTGGCTTCAATAAGCCTGGTGGACCCATGGATGAAGGACCAGATCTTGATCTAGGCCCA; CCTGTAGATCCAGATGAAGACTCTGACAACAGTGCAATTTATGTACAAGGATTAAATGACAGTGTGACTC; TAGATGATCTGGCAGACTTCTTTAAGCAGTGTGGGGTTGTTAAGATGAACAAGAGAACTGGGCAACCCAT; GATCCACATCTACCTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTGTCCTATGAAGACCCA; CCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGGAAAGATTTTCAAGGGAGCAAACTTAAAGTCTCCC; TTGCTCGGAAGAAGCCTCCAATGAACAGTATGCGGGGTGGTCTGCCACCCCGTGAGGGCAGAGGCATGCC; ACCACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGGACCCATGGGTCGCATGGGAGGCCGT; GGAGGAGATAGAGGAGGCTTCCCTCCAAGAGGACCCCGGGGTTCCCGAGGGAACCCCTCTGGAGGAGGAA; ACGTCCAGCACCGAGCTGGAGACTGGCAGTGTCCCAATCCGGGTTGTGGAAACCAGAACTTCGCCTGGAG; AACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTGAAGGCTTCCTCCCGCCACCCTTTCCGCCCCCGGGT; GGTGATCGTGGCAGAGGTGGCCCTGGTGGCATGCGGGGAGGAAGAGGTGGCCTCATGGATCGTGGTGGTC; CCGGTGGAATGTTCAGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTCCGTGGTGGCCGGGGCATGGACCG; AGGTGGCTTTGGTGGAGGAAGACGAGGTGGCCCTGGGGGGCCCCCTGGACCTTTGATGGAACAGATGGGA; GGAAGAAGAGGAGGACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTCAGGAGCGCAGAGATC; GGCCCTACTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005243 unedited; AGTCAGCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAAGGAGA; GAAAATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAG; TGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACA; AAGCTATGGAACCTATGGACAGCCCACTGATGTCAGCTATACCCAGGCTCAGACCACTGC; AACCTATGGGCAGACCGCCTATGCAACTTCTTATGGACAGCCTCCCACTGGTTATACTAC; TCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGA; TACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGCATATGG; CACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACC; GCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTACAA; CCAGCCCAGCCTAGGATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTA; CCCCATGCAGCCAGTCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACA; GCCGACTAGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACCGAGCAG; CTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGCAGCCTTCCACTAG; NTACCCACCCCCANACTGGATCCTACAGCCAAGCTNCAAGTCAATATAGCCCACAGAGCA; GCAGCTACGGGCAGCAGAAGTCATCCCGACAGGACCACCCCATAGCATGGGTGTTTATGG; GCAGGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005243 unedited; CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAACATTTGGAATTT; TATTTAAAAAAAAAAAAAAAACATCACAACCATGGAACATTGTTACAGTTAAGAAGGCCC; TCTTGGGTTCTCCACAATGATACTGGAGCATGCTCACAAGGGGCTTCCCATTGTTAAAGT; CTTAAACAACCATTTTTAAAAGAAGGAAGAAAAAAAAACTCCGCACACTACCATTTAACT; TGTTTTAATGTTTCTTCACAAATGGTGAAAAATACTAAAGTACAGACAAGGAATAATCAT; AATGTTGGGGCCAACATTATAAATATGGAATTATAAATTTAAAACATTTTCTGGTTTAAA; AAATAAATCTGGTAGTCAATGCAGCTCTGCGGGGTCTCTGCATCTAGTAGGGCCGATCTC; TGCGCTCCTGACGGTGCTCGCCTTTATCCATTTTTCCAGGTCCTCCACGTCCTCCTCTTC; TTCCTCCCATCTGTTCCATCAAAGGTCCAGGGGGCCCCCCAGGGCCACCTCGTCTTCCTC; CACCAAAGCCACCTCGGTCCATGCCCCGGCCACCACGGAAGCCACCTCTGTCTCCACCAC; GGCCACCTCTGAACATTCCACCGGGACCACCACGATCCATGAGGCCACCTCTTCCTCCCC; GCATGCCACCAAGGCCACCTCTGCCACGATCACCACCCGGGGGCGGAAAGGTTGGCCGAA; AGAAACCTTCAGCCTTTGGGGCCTTACACTGGTTGCACTCTGGTTCTCCAGCGAAGTTTT; GGTTTCCACACCCCGTATTGGGACACTGCAGTCTCCAACTCGGGCTGGACGTTTCTTCCC; AAAGGGTTCCCTCGGAACCCGGGGTCCTTTGAAGGGAACCTCCTTATTTCTTCAGCCTTC; TTGCACCAGGTTCCCAAGACTCCGGGCTCCTGACTCCCGAAGGGTGGGCTGCCCTGCCTA; CGGGGGCAAACCCCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005243
• Insert Size: 2330 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005243.1, NP_005234.1
• RefSeq Size: 2679 bp
• RefSeq ORF: 2679 bp
• Locus ID: 2130
• UniProt ID: Q01844
• Cytogenetics: 22q12.2
• Domains: RRM, zf-RanBP
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Summary: This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]; Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.