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GPR31 (NM_005299) Human Untagged Clone

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SKU
SC116805
GPR31 (untagged)-Human G protein-coupled receptor 31 (GPR31)
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GPR31
Synonyms 12-HETER; HETER; HETER1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_005299, RT-PCR generated
ATGCCATTCCCAAACTGCTCAGCCCCCAGCACTGTGGTGGCCACAGCTGTGGGTGTCTTG
CTGGGGCTGGAGTGTGGGCTGGGTCTGCTGGGCAACGCGGTGGCGCTGTGGACCTTCCTG
TTCCGGGTCAGGGTGTGGAAGCCGTACGCTGTCTACCTGCTCAACCTGGCCCTGGCTGAC
CTGCTGTTGGCTGCGTGCCTGCCTTTCCTGGCCGCCTTCTACCTGAGCCTCCAGGCTTGG
CATCTGGGCCGTGTGGGCTGCTGGGCCCTGCGCTTCCTGCTGGACCTCAGCCGCAGCGTG
GGGATGGCCTTCCTGGCCGCCGTGGCTTTGGACCGGTACCTCCGTGTGGTCCACCCTCGG
CTTAAGGTCAACCTGCTGTCTCCTCAGGCGGCCCTGGGGGTCTCGGGCCTCGTCTGGCTC
CTGATGGTCGCCCTCACCTGCCCGGGCTTGCTCATCTCTGAGGCCGCCCAGAACTCCACC
AGGTGCCACAGTTTCTACTCCAGGGCAGACGGCTCCTTCAGCATCATCTGGCAGGAAGCA
CTCTCCTGCCTTCAGTTTGTCCTCCCCTTTGGCCTCATCGTGTTCTGCAATGCAGGCATC
ATCAGGGCTCTCCAGAAAAGACTCCGGGAGCCTGAGAAACAGCCCAAGCTTCAGCGGGCC
CAGGCACTGGTCACCTTGGTGGTGGTGCTGTTTGCTCTGTGCTTTCTGCCCTGCTTCCTG
GCCAGAGTCCTGATGCACATCTTCCAGAATCTGGGGAGCTGCAGGGCCCTTTGTGCAGTG
GCTCATACCTCGGATGTCACGGGCAGCCTCACCTACCTGCACAGTGTGCTCAACCCCGTG
GTATACTGCTTCTCCAGCCCCACCTTCAGGAGCTCCTATCGGAGGGTCTTCCACACCCTC
CGAGGCAAAGGGCAGGCAGCAGAGCCCCCAGATTTCAACCCCAGAGACTCCTATTCCTGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005299 unedited
GTCGTATNCTGGTTACCGATCTTACTCGGGAAGGCGGCAGCCCGTGTGATGGAATCTGCA
GAATTCGGCTTGTGATGCCATTCCCAAACTGCTCAGCCCCCAGCACTGTGGTGGCCACAG
CTGTGGGTGTCTTGCTGGGGCTGGAGTGTGGGCTGGGTCTGCTGGGCAACGCGGTGGCGC
TGTGGACCTTCCTGTTCCGGGTCAGGGTGTGGAAGCCGTACGCTGTCTACCTGCTCAACC
TGGCCCTGGCTGACCTGCTGTTGGCTGCGTGCCTGCCTTTCCTGGCCGCCTTCTACCTGA
GCCTCCAGGCTTGGCATCTGGGCCGTGTGGGCTGCTGGGCCCTGCGCTTCCTGCTGGACC
TCAGCCGCAGCGTGGGGATGGCCTTCCTGGCCGCCGTGGCTTTGGACCGGTACCTCCGTG
TGGTCCACCCTCGGCTTAAGGTCAACCTGCTGTCTCCTCAGGCGGCCCTGGGGGTCTCGG
GCCTCGTCTGGCTCCTGATGGTCGCCCTCACCTGCCCGGGCTTGCTCATCTCTGAGGCCG
CCCAGAACTCCACCAGGTGCCACAGTTTCTACTCCAGGGCAGACGGCTCCTTCAGCATCA
TCTGGCAGGAAGCACTCTCCTGCCTTCAGTTTGTCCTCCCCTTTGGCCTCATCGTGTTCT
GCAATGCAGGCATCATCAGGGCTCTCCAGAAAAGACTCCGGGAGCCTGAGAAACAGCCCA
AGCTTCAGCGGGCCCAGGCACTGGTCACCTTGGTGGTGGTGCTGTTTGCTCTGTGCTTTC
TGCCCTGCTTCCTGGCCAGAGTCCTGATGCACATCTTCCAGAATCTGGNGAGCTGCANGG
CCCTTTGTGCAGTGGCTCATACCTCGGATGTCACGGGCAGCCTCACCTC
Restriction Sites Please inquire |
ACCN NM_005299
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005299.1, NP_005290.1
RefSeq Size 960 bp
RefSeq ORF 960 bp
Locus ID 2853
UniProt ID O00270
Cytogenetics 6q27
Protein Families Druggable Genome, GPCR, Transmembrane
Summary High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.UniProtKB/Swiss-Prot Function
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"NM_005299" in other vectors (4)
SKU Description Size Price
RC220560 GPR31 (Myc-DDK-tagged)-Human G protein-coupled receptor 31 (GPR31) 10 ug
$289.00 $300.00
RC220560L3 Lenti ORF clone of Human G protein-coupled receptor 31 (GPR31), Myc-DDK-tagged 10 ug
$600.00
RC220560L4 Lenti ORF clone of Human G protein-coupled receptor 31 (GPR31), mGFP tagged 10 ug
$600.00
RG220560 GPR31 (tGFP-tagged) - Human G protein-coupled receptor 31 (GPR31) 10 ug
$489.00 $500.00

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