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SFRS4 (SRSF4) (NM_005626) Human Untagged Clone

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SKU
SC116596
SRSF4 (untagged)-Human serine/arginine-rich splicing factor 4 (SRSF4)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SFRS4
Synonyms SFRS4; SRP75
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC116596 sequence for NM_005626 edited (data generated by NextGen Sequencing)
ATGCCGCGGGTGTACATCGGCCGCCTGAGCTACCAGGCCCGGGAGCGCGATGTGGAGCGC
TTCTTTAAGGGCTACGGGAAGATCCTGGAGGTGGATCTGAAGAACGGATATGGTTTTGTG
GAGTTTGATGATCTGCGTGATGCAGATGATGCTGTTTATGAACTGAATGGCAAAGACCTT
TGTGGTGAGCGAGTAATTGTTGAGCATGCCCGCGGCCCACGGCGAGATGGCAGTTACGGT
TCTGGACGCAGTGGATATGGTTATAGAAGAAGTGGCCGAGATAAATATGGCCCTCCTACT
CGCACAGAGTACAGACTTATTGTGGAGAATTTGTCAAGTCGGTGCAGCTGGCAAGACCTA
AAGGATTATATGCGTCAGGCAGGAGAAGTGACTTATGCAGATGCTCACAAGGGACGCAAA
AATGAAGGGGTGATTGAATTTGTATCTTATTCTGATATGAAAAGAGCTTTGGAAAAGTTG
GATGGAACTGAAGTCAATGGGAGAAAAATCAGATTAGTTGAAGACAAGCCAGGTTCCAGA
CGACGCCGGTCCTACTCCAGAAGCCGGAGTCATTCAAGGTCTCGCTCTCGAAGCAGACAT
TCCCGTAAGAGCAGAAGCCGAAGTGGCAGCAGCAAAAGCAGTCATTCTAAGAGTAGATCT
CGGTCCAGGTCGGGCTCCCGCTCCCGGAGCAAGAGCCGGAGCCGGAGCCAGAGTCGGAGC
CGGAGCAAGAAAGAGAAAAGCAGGAGCCCCAGCAAGGACAAGAGCCGCAGCCGCAGCCAT
AGCGCTGGCAAGAGCCGCAGCAAGAGCAAAGACCAAGCTGAAGAGAAGATCCAAAACAAT
GACAATGTCGGGAAACCCAAGAGCCGGAGTCCTAGCAGGCATAAAAGTAAGAGCAAAAGT
CGGAGCAGGAGTCAGGAGAGGAGAGTGGAGGAGGAGAAGCGAGGGAGTGTGAGCAGGGGC
AGGAGCCAGGAGAAGAGCCTCCGCCAGAGTCGGAGCCGGAGCAGGAGCAAAGCGGGCAGC
AGGAGCCGGAGCAGGAGCCGCAGCAAGAGCAAGGACAAGAGGAAGAGCAGGAAGAGAAGC
AGAGAGGAGAGCCGCAGTCGCAGTCGCAGCCGCAGCAAGAGTGAGAGGAGCAGAAAGCGA
GGCAGCAAGCGAGACAGCAAGGCGGGCAGCAGCAAGAAGAAGAAGAAGGAAGACACTGAC
CGCTCCCAGTCCAGATCTCCATCCCGCTCCGTGTCAAAGGAGCGGGAACATGCCAAGTCT
GAATCCAGCCAGAGGGAAGGTCGAGGAGAGAGTGAGAATGCTGGCACCAATCAGGAGACC
CGGTCCAGGTCGAGATCCAATTCCAAATCGAAACCAAACCTTCCATCAGAATCACGCTCC
AGATCAAAGTCAGCTTCAAAAACCCGATCTCGGTCCAAGTCTAGATCCAGGTCTGCTTCC
AGATCGCCCTCCCGATCTAGATCTAGGTCCCACTCAAGGTCCTAA

Clone variation with respect to NM_005626.4
759 a=>c;1013 g=>c;1066 g=>a
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005626 unedited
GTCAGGATTATGTAATACGACTTACNTATAGGGACGGCCGCGCAATTCGGCACGAGGGCC
GCCCGCCCGTTGCCGCCGCCGCCGCTGCCGCCGTGCTCTCGCTTTGCCCGCCGCCGCCTA
AGGGGGCTGGGGCCGGGGCCAGCCATCACTGCCGTTGCCGGGATGCCGCGGGTGTACATC
GGCCGCCTGAGCTACCAGGCCCGGGAGCGCGATGTGTTTTCGCTTCTTTAAGGGCTACGG
GAAGATCCTGGAGGTGGATCTGAAGAACGGATATGGTTTTGTGGAGTTTGATGATCTGCG
TGATGCAGATGATGCTGTTTATGAACTGAATGGCAAAGACCTTTGTGGTGAGCGAGTAAT
TGTTGAGCATGCCCGCGGCCCACGGCGAGATGGCAGTTACGGTTCTGGACGCAGTGGATA
TGGTTATAGAAGAAGTGGCCGAGATAAATATGGCCCTCCTACTCGCACAGAGTACAGACT
TATTGTGGAGAATTTGTCAAGTCGGTGCAGCTGGCAAGACCTAAAGGATTATATGCGTCA
GGCAGGAGAAGTGACTTATGCAGATGCTCACAAGGGACGCAAAAATGAAGGGGTGATCGA
ATTTGTATCTTATTCTGATATGAAAAGAGCTTTGGAAAAGTTGGATGGAACTGAAGTCAA
TGGGAGAAAAATCAGATTAGTTGAAGACAAGCCAGGTTCCAGACGACGCCGGTCCTACTC
CAGAGCCGGAGTCATTCAAAGTCTCGCTCTCGAAGCAGACATTCCCGGTAGAGCAGAAGC
CGAAGTGCCAGCACCAAAAGCAGTCATTCTAAGAGTAGATCCTCGTCCAAGTCGGGCTCC
CGCTCCCGGAGCAGAGCCGNACCCGAGCCANAGTTCGACCGGAGCAAGAAAGAAAAAGCA
GAGCCCCACAAGACAANAGCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005626 unedited
GCGGCACGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTATATAAAACAATATAATCA
CAATACCAGAATATGGAACTCTACAGTTTATTTCCTATGGGTTACTGCAGGTATCAATTT
TCCTCGACTGTGCTATTCACAACTTTGTTAAGTCCAAAAATATGAAACCAAAGTGGTAGG
AAACTTAAGACTTAGCACTTTCACTAAATGGCATACATCGAAGGGCATCAATTCAAGAGC
AAAAATGGTTGAACTCACCATACCTACCTATGTGGTCATTCCAGCCTTAGAGCCGTCCAG
GTTACTGAGCTCCCTGTAGGAAAGGCCAGGCCTGAAAGCCAAGGCGTTTTGGATATTCTA
CTGTGGGCCATGAGTAAAAGGGGATTTTCAAAGAGAAAATTTTTTTCAGTCGAGCAGGAA
GGCCTGGTGCCAGGAGGCTTTACTGAGAGGAAGCACTTAGATTTAACAATTATAGACACA
CCATTAGGGGAGTTAAAAATGTACAGCAGTGACATGTTCTACTCCAATCACTTGTGCTAC
GGCTACCAAACATGTACAAAAGACTTCTCGGGTAGTTCCAGCTGTGGCCATAGCCAGTTA
GGACCTTGAGTGGGACCTAGATCTAGATCGGGAGGGCGATCTGGAAGCAGACCTGGATCT
AGACTTGGACCGAGATCGGGTTTTTGAAGCTGACTTTGATCTGGAGCGTGATTCTGATGG
AAGGTTTGGTTTTCGAATTGGAATTGGATCTCGACCTGGACCGGGGTCTCTGATGGNTGC
CAGCATTCTCACTCTCTCCTCGACCTTTCCTCTGGCTGGATTCAGACTGGNCATGGTCCC
CGCTCCTTTGACACGAGCGGNATGGANATCTGGACTGGGAGCGGNCANNNGNCTCCCCTC
TCTTCTTCTTGCTGCTGCCGCCCTGCTGNCCGCTTGCTGCTCGCTTCTGCTCTCTAC
Restriction Sites NotI-NotI |
ACCN NM_005626
Insert Size 2350 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005626.3, NP_005617.2
RefSeq Size 2158 bp
RefSeq ORF 1485 bp
Locus ID 6429
UniProt ID Q08170
Cytogenetics 1p35.3
Domains RRM
Protein Pathways Spliceosome
Summary This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. provided by RefSeq, Feb 2009
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"NM_005626" in other vectors (7)
SKU Description Size Price
RC201227 SRSF4 (Myc-DDK-tagged)-Human serine/arginine-rich splicing factor 4 (SRSF4) 10 ug
$289.00 $457.00
RC201227L1 Lenti ORF clone of Human serine/arginine-rich splicing factor 4 (SRSF4), Myc-DDK-tagged 10 ug
$757.00
RC201227L2 Lenti ORF clone of Human serine/arginine-rich splicing factor 4 (SRSF4), mGFP tagged 10 ug
$757.00
RC201227L3 Lenti ORF clone of Human serine/arginine-rich splicing factor 4 (SRSF4), Myc-DDK-tagged 10 ug
$757.00
RC201227L4 Lenti ORF clone of Human serine/arginine-rich splicing factor 4 (SRSF4), mGFP tagged 10 ug
$757.00
RG201227 SRSF4 (tGFP-tagged) - Human serine/arginine-rich splicing factor 4 (SRSF4) 10 ug
$489.00 $657.00
SC320685 SRSF4 (untagged)-Human serine/arginine-rich splicing factor 4 (SRSF4) 10 ug
$457.00

file-alt Citations

FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis
Zhao, Yang, Sun et al
Cell Res. (2014) 24 (12), 1403-19 DOI: 10.1038/cr.2014.151
Cited by 1247
AI Summary: In this study, OriGene products contributed human SR plasmids, which were used to investigate the role of serine/arginine-rich proteins in FTO-dependent mRNA splicing and adipogenesis.
Snippet: Cell line, plasmids and antibodies. 3T3-L1 pre-adipocytes were obtained from ATCC (Manassas, VA). The human FTO and mouse RUNX1T1 genes were cloned into pEGFP-C1B 56 . Human SR plasmids were bought from OriGene Technologies (Rockville, MD). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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