MFGE8 (NM_005928) Human Untagged Clone

SKU SC116455

MFGE8 (untagged)-Human milk fat globule-EGF factor 8 protein (MFGE8), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: MFGE8
• Synonyms: BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8; MFGM; OAcGD3S; SED1; SPAG10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005928, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCCGCGCCCCCGCCTGCTGGCCGCGCTGTGCGGCGCGCTGCTCTGCGCCCCCAGCCTCCTCGTCGCCC; TGGATATCTGTTCCAAAAACCCCTGCCACAACGGTGGTTTATGCGAGGAGATTTCCCAAGAAGTGCGAGG; AGATGTCTTCCCCTCGTACACCTGCACGTGCCTTAAGGGCTACGCGGGCAACCACTGTGAGACGAAATGT; GTCGAGCCACTGGGCCTGGAGAATGGGAACATTGCCAACTCACAGATCGCCGCCTCGTCTGTGCGTGTGA; CCTTCTTGGGTTTGCAGCATTGGGTCCCGGAGCTGGCCCGCCTGAACCGCGCAGGCATGGTCAATGCCTG; GACACCCAGCAGCAATGACGATAACCCCTGGATCCAGGTGAACCTGCTGCGGAGGATGTGGGTAACAGGT; GTGGTGACGCAGGGTGCCAGCCGCTTGGCCAGTCATGAGTACCTGAAGGCCTTCAAGGTGGCCTACAGCC; TTAATGGACACGAATTCGATTTCATCCATGATGTTAATAAAAAACACAAGGAGTTTGTGGGTAACTGGAA; CAAAAACGCGGTGCATGTCAACCTGTTTGAGACCCCTGTGGAGGCTCAGTACGTGAGATTGTACCCCACG; AGCTGCCACACGGCCTGCACTCTGCGCTTTGAGCTACTGGGCTGTGAGCTGAACGGATGCGCCAATCCCC; TGGGCCTGAAGAATAACAGCATCCCTGACAAGCAGATCACGGCCTCCAGCAGCTACAAGACCTGGGGCTT; GCATCTCTTCAGCTGGAACCCCTCCTATGCACGGCTGGACAAGCAGGGCAACTTCAACGCCTGGGTTGCG; GGGAGCTACGGTAACGATCAGTGGCTGCAGGTGGACCTGGGCTCCTCGAAGGAGGTGACAGGCATCATCA; CCCAGGGGGCCCGTAACTTTGGCTCTGTCCAGTTTGTGGCATCCTACAAGGTTGCCTACAGTAATGACAG; TGCGAACTGGACTGAGTACCAGGACCCCAGGACTGGCAGCAGTAAGATCTTCCCTGGCAACTGGGACAAC; CACTCCCACAAGAAGAACTTGTTTGAGACGCCCATCCTGGCTCGCTATGTGCGCATCCTGCCTGTAGCCT; GGCACAACCGCATCGCCCTGCGCCTGGAGCTGCTGGGCTGTTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005928 unedited; GGTCAGCATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGTCTGAGC; AGCCCAGCGTGCCCATTCCAGCGCCCGCGTCCCCGCAGCATGCCGCGCCCCCGCCTGCTG; GCCGCGCTGTGCGGCGCGCTGCTCTGCGCCCCCAGCCTCCTCGTCGCCCTGGATATCTGT; TCCAAAAACCCCTGCCACAACGGTGGTTTATGCGAGGAGATTTCCCAAGAAGTGCGAGGA; GATGTCTTCCCCTCGTACACCTGCACGTGCCTTAAGGGCTACGCGGGCAACCACTGTGAG; ACGAAATGTGTCGAGCCACTGGGCCTGGAGAATGGGAACATTGCCAACTCACAGATCGCC; GCCTCGTCTGTGCGTGTGACCTTCTTGGGTTTGCAGCATTGGGTCCCGGAGCTGGCCCGC; CTGAACCGCGCAGGCATGGTCAATGCCTGGACACCCAGCAGCAATGACGATAACCCCTGG; ATCCAGGTGAACCTGCTGCGGAGGATGTGGGTAACAGGTGTGGTGACGCAGGGTGCCAGC; CGCTTGGCCAGTCATGAGTACCTGAAGGCCTTCAAGGTGGCCTACAGCCTTAATGGACAC; GAATTCGATTTCATCCATGATGTTAATAAAAAACACAAGGAGTTTGTGGGTAACTGGAAC; AAAAACGCGGTGCATGTCAACCTGTTTGAGACCCCTGTGGAGGCTCAGTACGTGAGATTG; TACCCCACGAGCTGCCACACGGCCTGCACTCTGCGCTNTGAGCTACTGGGCTGTGAGCTG; AACGGATGCGCCAATTCCCCTGGCCTGAAGAATAACAGCATCCTGACAAGCAGACACGGC; CTTCAGCAGCTACAAGACCTGGNGCTNGCATCTCTTCAGCTGGAACCCTNCTATGCACGG; CTGGCAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005928 unedited; TGAAGTAATATTTTTGGGAGCTGCCTGTGTCCTCTGGGGTNGAAAAGCCAGCATAGAGGG; CCCCAAACGCCCACCCTGCCAACCCATGCCCTCCTCTGACCTCGCTCCCTTTCTCCCCAT; AGACCCGCCCCACCCCCTTCTGTGTCGCTGGGCTTCAGGACAAGGGGCAAGAGAGGCAAC; CAGGGAGACAAGGGGAAGTGTCTTGGGGACAGGGGGCCACAGCAGTTGTGGCCACATGGT; ACCCCAGGGCCGACGCAGGGCCGACGGGCAAGAGGCTGTTATCTCCAGCCTGGCGCTTCC; GGGCCTTTCTTGAGGCCACCATGGGAATGTGATGTGTGAGAGAGGGGCTAGGAGAGACAG; AGACACACGCACCTGGGATCGGCGGTCCGGACAGGGGCAGGGAAACCACACGCCCTACTT; TGCCCTTTCCTGTCCATCCCAGACCTACTCAGATCCCTCAGTGCCTAAGAAAACAGGACA; GTGAGGACTGGGGGTTAGGGGACGGGGCTTANGGGCTGGGGCAGGGCCCGTGAGAGGTGG; AGGGTGGGAAAGAGGGAGGGAGGGGTGACTGTGTGGTGGNGNNTGCTTTGACACCCTTCC; CTTNTCCAGTTCCCAGCCCTATGGGTGTTTAAAGGGGCTGATAAACCAACAAGCAGCCGG; GCCCCTGTGAAGCCAGAAACACCTGGCGGGGAGCCGGTGGGCCCTAAACAGCCCAGCAGC; TTCAGGACCCTCGGCACGCTGTTGTCGCACGTTTCATGCCTGATGCGCTCTTTANNAACT; CCCTTTCGTCTTTCCACCCAGCTTTTTTTGCGGGAGAGGGTTTTCCCANCAGCCAGGAAN; ACNTTACCGCGCGCCACCCTGGGCTCTTGGACCTCTACAGCTCCACTTTATTCGCCGCTT; ACATCTCNNNNNNCACTATATTGCAGCGTACAACNCCCGNCCCCCGCGGGCGGAGTCCGC; CCCTCTCTNNACGCCCCACCCACCCGACCCTCCTCGTATCCNCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_005928
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005928.1, NP_005919.1
• RefSeq Size: 1934 bp
• RefSeq ORF: 1164 bp
• Locus ID: 4240
• UniProt ID: Q08431
• Cytogenetics: 15q26.1
• Domains: EGF, F5_F8_type_C
• Summary: This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]; Transcript Variant: This variant (1) encodes the longest isoform (a).