CPNE6 (NM_006032) Human Untagged Clone

SKU SC116368

CPNE6 (untagged)-Human copine VI (neuronal) (CPNE6)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CPNE6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116368 sequence for NM_006032 edited (data generated by NextGen Sequencing); ATGTCGGACCCTGAGATGGGATGGGTGCCTGAGCCCCCAACCATGACGCTGGGGGCCTCT; CGGGTGGAGCTGCGGGTGTCCTGCCATGGCCTCCTGGACCGGGACACACTCACCAAACCC; CACCCCTGCGTGCTGCTCAAGCTCTACTCTGATGAGCAGTGGGTGGAGGTAGAGCGCACA; GAGGTGCTTCGCTCCTGTTCCAGCCCTGTCTTCTCCCGGGTGCTGGCCCTTGAGTATTTT; TTTGAGGAGAAGCAGCCCCTGCAGTTCCACGTGTTCGATGCCGAGGACGGAGCCACCAGC; CCCCGAAATGATACCTTCCTCGGCTCTACGGAGTGCACCTTGGGCCAGATTGTGTCACAA; ACCAAGGTCACTAAGCCATTATTGCTGAAGAATGGGAAGACTGCGGGCAAGTCCACCATC; ACGATCGTGGCCGAGGAGGTATCAGGCACAAACGACTATGTGCAACTCACCTTCAGAGCC; TACAAGCTGGACAACAAGGATCTGTTCAGCAAGTCTGACCCTTTCATGGAAATCTATAAG; ACCAACGAGGACCAAAGTGATCAGCTCGTCTGGAGAACTGAGGTGGTGAAGAACAACCTG; AACCCCAGCTGGGAGCCGTTCCGCCTGTCCCTGCATTCCCTATGCAGCTGTGATGTTCAC; CGACCTCTCAAGTTCCTGGTGTATGACTATGACTCCAGTGGGAAGCATGACTTCATCGGC; GAGTTCACCAGCACTTTCCAGGAGATGCAGGAAGGGACGGCAAACCCTGGGCAGGAGATG; CAGTGGGACTGTATCAACCCCAAGTATCGGGACAAGAAGAAGAATTACAAGAGCTCAGGG; ACGGTAGTGCTGGCCCAGTGCACGGTGGAGAAGGTGCACACCTTCCTGGATTACATCATG; GGTGGCTGCCAGATCAGCTTCACGGTGGCCATCGACTTCACCGCCTCCAATGGGGACCCG; AGGAGCAGCCAGTCCCTGCACTGCCTCAGTCCCCGACAGCCCAACCACTACCTGCAGGCC; CTGCGTGCAGTGGGAGGCATCTGCCAGGACTATGACAGTGATAAGCGGTTCCCAGCTTTT; GGCTTTGGGGCTCGAATCCCCCCCAACTTCGAGGTGTCCCATGACTTTGCTATCAACTTT; GACCCGGAAAATCCTGAATGTGAAGAGATCTCAGGGGTCATCGCCTCCTACCGTCGTTGC; CTGCCCCAGATCCAGCTCTACGGCCCCACCAATGTGGCCCCCATCATCAACCGTGTGGCT; GAGCCGGCCCAGCGGGAGCAGAGCACCGGCCAAGCCACGAAGTACTCGGTGCTGCTGGTG; CTCACTGACGGTGTGGTGAGCGACATGGCTGAGACTCGCACTGCTATCGTGCGTGCCTCC; CGCCTGCCCATGTCCATCATCATCGTAGGCGTGGGCAATGCTGACTTCTCTGACATGCGG; CTGCTGGATGGCGACGACGGCCCCTTGCGCTGCCCCCGAGGGGTGCCTGCAGCCCGAGAC; ATTGTCCAGTTCGTGCCCTTCCGAGACTTCAAGGATGCTGCCCCCTCTGCACTCGCCAAG; TGTGTCCTGGCTGAGGTGCCACGGCAGGTGGTGGAGTACTACGCCAGCCAGGGCATCAGC; CCTGGGGCTCCCAGGCCCTGCACACTGGCTACGACTCCCAGCCCTAGCCCGTGA; ; Clone variation with respect to NM_006032.2; 567 g=>c;933 t=>c;942 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006032 unedited; NGGGTTAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCC; GGAGCTGGAGCCGGCGCCAGCGGCTGGAGCAGCAAGGGAGTCAGGGCCAGGGCCAGAGAG; CCGGAGAGAGGAGCCCCCGACCGAGAGCCCAGTGACATGTCGGACCCTGAGATGGGATGG; GTGCCTGAGCCCCCAACCATGACGCTGGGGGCCTCTCGGGTGGAGCTGCGGGTGTCCTGC; CATGGCCTCCTGGACCGGGACACACTCACCAAACCCCACCCCTGCGTGCTGCTCAAGCTC; TACTCTGATGAGCAGTGGGTGGAGGTAGAGCGCACAGAGGTGCTTCGCTCCTGTTCCAGC; CCTGTCTTCTCCCGGGTGCTGGCCCTTGAGTATTTTTTTGAGGAGAAGCAGCCCCTGCAG; TTCCACGTGTTCGATGCCGAGGACGGAGCCACCAGCCCCCGAAATGATACCTTCCTCGGC; TCTACGGAGTGCACCTTGGGCCAGATTGTGTCACAAACCAAGGTCACTAAGCCATTATTG; CTGAAGAATGGGAAGACTGCGGGCAAGTCCACCATCACGATCGTGGCCGAGGAGGTATCA; GGCACAAACGACTATGTGCAACTCACCTTCAGAGCCTACAAGCTGGACAACAAGGATCTG; TTCAGCAAGTCTGACCCTTTCATGGAAATCTATAAGACCAACGAGGACCAAAGTGATCAG; CTCGTCTGGAGAACTGAGGTGGTGAAGAACAACCTGAACCCCAGCTGGGAGCCGTTCCGC; CTGTCCCTGCATTNCCTATGCAGCTGTGATGTTCACCGACCTCTCAAGTCCTGGTGTATG; ACTATGACTCCAGTGGGAAGCATGACTNCATCGGCGAGTTCACCACACTTTCCACGAGAG; CAGGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006032 unedited; GACAGTTCCTTCTTTTTATAATGGGCTAGNAAAGTCAGATCACCCAAGGAAGTCACTGAG; GGGCCACAGCATTGAAGGGTATGGGGTTTGGAGAGATAGGAGCAGGACCCACCACTCACG; TCCAGAACCCAGGGGGCACACCTGGTCCAAGAGGTGGAGGCATTGGTCACTGGAGTCACG; AGGGTCAGGACAGGCACTGAGAGGCTGAGGGAGTGTCGGTCCGGAGGGAGGCAGTCACGG; GCTAGGGCTGGGAGTCGTAGCCAGTGTGCAGGGCCTGGGAGCCCCAGGGCTGATGCCCTG; GCTGGCGTAGTACTCCACCACCTGCCGTGGCACCTCAGCCAGGACACACTTGGCGAGTGC; AGAGGGGGCAGCATCCTTGAAGTCTCGGAAGGGCACGAACTGGACAATGTCTCGGGCTGC; AGGCACCCCTCGGGGGCAGCGCAAGGGGCCGTCGTCGCCATCCAGCAGCCGCATGTCAGA; GAAGTCAGCATTGCCCACGCCTACGATGATGATGGACATGGGCAGGCGGGAGGCACGCAC; GATAGCAGTGCGAGTCTCAGCCATGTCGCTCACCACACCGTCAGTGAGCACCAGCAGCAC; CGAGTACTTCGTGGCTTGGCCGGTGCTCTGCTCCCGCTGGGCCCGCTCAGCCACACGGTT; GATGATGGGGGCCACATNTGTGGGGCCGTANAGCTGGATCTGGGGCAGGCAACGACGGTA; GGAGGCGATGACCCCTGAGATCTCTTCACATTCAGGATTTTCCGGGTCAAAGTTGATAGC; AAAGTCATGGGACACCTCGAAGTTGGGGGGGATTTCAGCCCCAAAGCCAAAGCTGGGAAC; CGGTTTCACTGTCATAGTCCTGGCAGATGCCTNCCATGCACCCAGGGCCTGCAAGTATTG; GTTGGGCTTTCCGGGACTTGAGCAATGCAAGGCCTGGTTGCTTCCTCGGTTCCCATTGNA; GGCCGGGAAATCATTGCCCACTTGAACTTGATCTGCCGCCACCCTGATTAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006032
• Insert Size: 2240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006032.2, NP_006023.1
• RefSeq Size: 2018 bp
• RefSeq ORF: 1674 bp
• Locus ID: 9362
• UniProt ID: O95741
• Cytogenetics: 14q11.2
• Domains: C2, VWA
• Summary: This gene encodes a member of the copine family. Members of this family are calcium-dependent, phospholipid-binding proteins with C2 domains, two calcium- and phospholipid-binding domains. Through their domain structure and lipid binding capabilities, these proteins may play a role in membrane trafficking. This protein is thought to be brain-specific and has a domain structure of two N-terminal C2 domains and one von Willebrand factor A domain. It may have a role in synaptic plasticity. [provided by RefSeq, Jul 2013]; Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region and initiates translation at a downstream start codon,, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.