Hsp105 (HSPH1) (NM_006644) Human Untagged Clone

SKU SC115932

HSPH1 (untagged)-Human heat shock 105kDa/110kDa protein 1 (HSPH1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Hsp105
• Synonyms: HSP105; HSP105A; HSP105B; NY-CO-25
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115932 sequence for NM_006644 edited (data generated by NextGen Sequencing); ATGTCGGTGGTGGGGTTGGACGTGGGCTCGCAGAGCTGCTACATCGCGGTAGCCCGGGCC; GGGGGCATCGAGACCATCGCCAATGAGTTCAGCGACCGGTGCACCCCGTCAGTCATATCA; TTTGGATCAAAAAATAGAACAATCGGAGTTGCAGCCAAAAATCAGCAAATCACTCATGCA; AACAATACGGTGTCTAACTTCAAAAGATTTCATGGCCGAGCATTCAACGACCCCTTCATT; CAAAAGGAGAAGGAAAACTTGAGTTACGATTTGGTTCCATTGAAAAATGGTGGAGTTGGA; ATAAAGGTAATGTACATGGGTGAAGAACATCTATTTAGTGTGGAGCAGATAACAGCCATG; TTGTTGACTAAGCTGAAGGAAACTGCTGAAAACAGCCTCAAGAAACCAGTAACAGATTGT; GTTATTTCAGTCCCCTCCTTCTTTACAGATGCTGAGAGGCGATCTGTGTTAGATGCTGCA; CAGATTGTTGGCCTAAACTGTTTAAGACTTATGAATGACATGACAGCTGTTGCTTTGAAT; TACGGAATTTATAAGCAGGATCTCCCAAGCCTGGATGAGAAACCTCGGATAGTGGTTTTT; GTTGATATGGGACATTCAGCTTTTCAAGTGTCTGCTTGTGCTTTTAACAAGGGAAAATTG; AAGGTACTGGGAACAGCTTTTGATCCTTTCTTAGGAGGAAAAAACTTCGATGAAAAGTTA; GTGGAACATTTCTGTGCAGAATTTAAAACTAAGTACAAGTTGGATGCAAAATCCAAAATA; CGAGCACTCCTACGTCTGTATCAGGAATGTGAAAAACTGAAAAAGCTAATGAGCTCTAAC; AGCACAGACCTTCCACTGAATATCGAATGCTTTATGAATGATAAAGATGTTTCCGGAAAG; ATGAACAGGTCACAATTTGAAGAACTCTGTGCTGAACTTCTGCAAAAGATAGAAGTACCC; CTTTATTCACTGTTGGAACAAACTCATCTCAAAGTAGAAGATGTGAGTGCAGTTGAGATT; GTTGGAGGCGCTACACGAATTCCAGCTGTGAAGGAAAGAATTGCCAAATTCTTTGGAAAA; GATATTAGCACAACACTCAATGCAGATGAAGCAGTAGCCAGAGGATGTGCATTACAGTGT; GCAATACTTTCCCCGGCATTTAAAGTTAGAGAATTTTCCGTCACAGATGCAGTTCCTTTT; CCAATATCTCTGATCTGGAACCATGATTCAGAAGATACTGAAGGTGTTCATGAAGTCTTT; AGTCGAAACCATGCTGCTCCTTTCTCCAAAGTTCTCACCTTTCTGAGAAGGGGGCCTTTT; GAGCTAGAAGCTTTCTATTCTGATCCCCAAGGAGTTCCATATCCAGAAGCAAAAATAGGC; CGCTTTGTAGTTCAGAATGTTTCTGCACAGAAAGATGGAGAAAAATCTAGAGTAAAAGTC; AAAGTGCGAGTCAACACCCATGGCATTTTCACCATCTCTACGGCATCTATGGTGGAGAAA; GTCCCAACTGAGGAGAATGAAATGTCTTCTGAAGCTGACATGGAGTGTCTGAATCAGAGA; CCACCAGAAAACCCAGACACTGATAAAAATGTCCAGCAAGACAACAGTGAAGCTGGAACA; CAGCCCCAGGTACAAACTGATGCTCAACAAACCTCACAGTCTCCCCCTTCACCTGAACTT; ACCTCAGAAGAAAACAAAATCCCAGATGCTGACAAAGCAAATGAAAAAAAAGTTGACCAG; CCTCCAGAAGCTAAAAAGCCCAAAATAAAGGTGGTGAATGTTGAGCTGCCTATTGAAGCC; AACTTGGTCTGGCAGTTAGGGAAAGACCTTCTTAACATGTATATTGAGACAGAGGGTAAG; ATGATAATGCAAGATAAATTGGAAAAAGAAAGGAATGATGCTAAAAATGCAGTTGAGGAA; TATGTGTATGAGTTCAGAGACAAGCTGTGTGGACCATATGAAAAATTTATATGTGAGCAG; GATCATCAAAATTTTTTGAGACTCCTCACAGAAACTGAAGACTGGCTGTATGAAGAAGGA; GAGGACCAAGCTAAACAAGCATATGTTGACAAGTTGGAAGAATTAATGAAAATTGGCACT; CCAGTTAAAGTTCGGTTTCAGGAAGCTGAAGAACGGCCAAAAATGTTTGAAGAACTAGGA; CAGAGGCTGCAGCATTATGCCAAGATAGCAGCTGACTTCAGAAATAAGGATGAGAAATAC; AACCATATTGATGAGTCTGAAATGAAAAAAGTGGAGAAGTCTGTTAATGAAGTGATGGAA; TGGATGAATAATGTCATGAATGCTCAGGCTAAAAAGAGTCTTGATCAGGATCCAGTTGTA; CGTGCTCAGGAAATTAAAACAAAAATCAAGGAATTGAACAACACATGTGAACCCGTTGTA; ACACAACCGAAACCAAAAATTGAATCACCCAAACTGGAAAGAACTCCAAATGGCCCAAAT; ATTGATAAAAAGGAAGAAGATTTAGAAGACAAAAACAATTTTGGTGCTGAACCTCCACAT; CAGAATGGTGAATGTTACCCTAATGAGAAAAATTCTGTTAATATGGACTTGGACTAG; ; Clone variation with respect to NM_006644.2; 228 t=>c;732 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006644 unedited; ATACGACTACTATAGGGCGGCCGCGAATCGGCACGAGGGGCTGAGGAAGTGGGACCTCCC; CTTTTGGGTCGGTAGTTCAGCGCCGGCGCCGGTGTGCGAGCCGCGGCAGAGTGAGGCAGG; CAACCCGAGGTGCGGAGCGACCTGCGGAGGCTGAGCCCCGCTTTCTCCCAGGGTTTCTTA; TCAGCCAGCCGCCGCTGTCCCCGGGGGAGTAGGAGGCTCCTGACAGGCCGCGGCTGTCTG; TGTGTCCTTCTGAGTGTCAGAGGAACGGCCAGACCCCGCGGGCCGGAGCAGAACGCGGCC; AGGGCAGAAAGCGGCGGCAGGAGAAGCAGGCAGGGGGCCGGAGGACGCAGACCGAGACCC; GAGGCGGAGGCGGACCGCGAGCCGGCCATGTCGGTGGTGGGGTTGGACGTGGGCTCGCAG; AGCTGCTACATCGCGGTAGCCCGGGCCGGGGGCATCGAGACCATCGCCAATGAGTTCAGC; GACCGGTGCACCCCGTCAGTCATATCATTTGGATCAAAAAATAGAACAATCGGAGTTGCA; GCCAAAAATCAGCAAATCACTCATGCAAACAATACGGTGTCTAACTTCAAAAGATTTCAT; GGCCGAGCATTCAACGACCCCTTCATTCAAAAGGAGAAGGAAAACTTGAGTTACGATTTG; GTTCCATTGAAAAATGGTGGAGTTGGAATAAGGTAATGTACATGGGTGAAGACATCTATN; TAGTGTGGAGCAGATACAGCCATGTTGNTGACTAAGCTGAAGGAAACTGCTGAAAACAGC; CTCAAGAACCAGTNNACAGATGTGTATTTCAGTCCCCTCCTTCTTTACGATGCTGAGAGG; CGATCTGTGNTAGATGCTGCACAGATGGNTGCCCTAACTGTNTAGAACTATGAATGACAT; GACAGCTGTGCTTTGATTACGGAATTATAANCAGACTCCCAGCCTGATGAGAACTCGGGA; AGGGGGGTTTGTGGAATGGAAANTCACCTTNCAGGGTCTGCTGTGCTTACAGGAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006644 unedited; GCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTGGAAAGAC; ACCATAAGATGAACTTTATTTTAAAGCTCATAAAAGTGTTCACAATCAGTTACAACAGGA; TCGACATTTCTTCCATTCCACACTTTCACATGACAATATACTGTATAGTGAGAGAGAAAG; TTTAAAGTTTTTGTTCTGCATGCTGCTAACACATTTGACTAGCTTTTGTTTTACTCATTG; AATTTTTAATATCAAAGCAAAAAGTCATTTTCTCTTGGACAGAAATGGTTTTAGAAAGCC; CTTATGAAGTCAGACTTAGTCTTGTTTATAAACATCCACACCCACACACATGCTGAATGG; AGAGCAAAATGCAAGAAAACTACCTTGGCAGGAACAAATGCTTAAAGATTTTAATCACAG; CCCTCTTGAACAAGCAGTACAGTTTTTTTTCTCCAAAAGACAAAAGTCAGTTTCATCCTC; AGTGATGCAAATGGTGAGACTGCACAGAAAGCTTAGTATGAATTCACAATTCCACAGGAA; TCTGAAAGTTACCAAGGCAATTTTCCCTTTTAGGATCATAAAGACTACAGACTTAAGCTT; TTTTTCTTTTTCCATATAATACACAAAATTTCTAAATATCCTTAAAAAAGAAAATATAAA; TAGTTTCAGTATGTTATGTAGAGTCACATACTATGGCAAAAATATTTTATTAATTGAAGG; AATAGGCCAATTTAAGGTTATCTAGTCCAAGTCCATATTAACAGAATTTTTCTCATTAGG; GTAACATTCACCATTCTGTGGNGGGNAGGTTCAGCACCAAAAATGGNGTTTTGGNCTTCT; AAATCTTCTTCCTTTTTATCAATNATTTGGCCAATTTTGGNAGTCTTTTCCAGTTTGGGT; GAGTCAAATNTTGGGNTTTCGCGTTGGGGTACAACNGGGNTCACAAGGNGGTGNTCAATT; CCCTTGAATTTTGGTTTAATTCCCTGAGCCGTACACCTGGAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_006644
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006644.2, NP_006635.2
• RefSeq Size: 3680 bp
• RefSeq ORF: 2577 bp
• Locus ID: 10808
• UniProt ID: Q92598
• Cytogenetics: 13q12.3
• Domains: HSP70
• Protein Families: Stem cell - Pluripotency
• Summary: This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]; Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1, also known as Hsp105alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.