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GLO1 (NM_006708) Human Untagged Clone

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SKU
SC115924
GLO1 (untagged)-Human glyoxalase I (GLO1)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GLO1
Synonyms GLOD1; GLYI; HEL-S-74
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115924 sequence for NM_006708 edited (data generated by NextGen Sequencing)
ATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTCAGTTGCTGC
TCCGACGCGGACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTACGAGTGAAG
GATCCTAAGAAGTCACTGGATTTTTATACTAGAGTTCTTGGAATGACGCTAATCCAAAAA
TGTGATTTTCCCATTATGAAGTTTTCACTCTACTTCTTGGCTTATGAGGATAAAAATGAC
ATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCTCCAGAAAAGCTACACTTGAG
CTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAATGGCAATTCA
GACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGTGCTTGTAAA
AGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAAATGAAAGGC
CTGGCATTTATTCAAGATCCTGATGGCTACTGGATTGAAATTTTGAATCCTAACAAAATG
GCAACCTTAATGTAG

Clone variation with respect to NM_006708.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006708 unedited
GTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGTCGTCTGTGATACTGC
AGCGCAGCCATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTC
AGTTGCTGCTCCGACGCGGACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTA
CGAGTGAAGGATCCTAAGAAGTCACTGGATTTTTATACTAGAGTTCTTGGAATGACGCTA
ATCCAAAAATGTGATTTTCCCATTATGAAGTTTTCACTCTACTTCTTGGCTTATGAGGAT
AAAAATGACATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCTCCAGAAAAGCT
ACACTTGAGCTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAAT
GGCAATTCAGACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGT
GCTTGTAAAAGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAA
ATGAAAGGCCTGGCATTTATTCAAGATCCTGATGGCTACTGGATTGAAATTNTGAATCCT
AACAAAATGGCAACCTTAATGTAGTGCTGTGAGAATTCTCCTTTGAGATTTCAGAAGAAA
GGAAACAATGTGATTCAAGATATTTACATACCAGAAGCATCTAGGACTGATGGATCACTG
TCCCCGATCNATTATTCTTCAGTCCATTTCCCCTTCCTATNTCAGCTGTTCCTTTTCACC
TAACTGTTCAGTCATTCTGGTTTTCAAGCAGTGCTTTATCTCATGTCCTGAATATAGNTG
NNGTACTTTATTTTTTAGTAATAATANAACAGTTCCCTTCAGAGCTGCATTTGCCTCTTC
TGCCACTAAATATTNACTCCCTTCAATCTGCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006708 unedited
GGTTTTTCAGGGGNNCTNTNATNTATTCCAGTTCTCTCAAGCTATTGGCTATCTTGTCTT
AAAGCACTCACATAAATCCATTTCACCCAAAAAGGAAACATAAAGTGCTTCTAGCAGTAC
AAGCACGGTTGGCATGGCCTTTCCAAAGGTCTTCCACTAGAGTCTAGAGAAATCTAAATA
TAGTCATCCACAAACTGGATGTTTTTATTTTCTGAGCCATTAGAGATTTTCAAAATCACT
TTGATTTTTAAAAACTCATCAAATGTGAATCATGGCGGGGAAGACCACTGAGCTGATTTC
TGATAACTAAGTTATCACTGAACATAATTTATCATATATGGCTACTGGCATCATGAAGAC
CTTGGGATAGGGAAGACTCTTTATGAGAAATATAAACATCACTTGTGTAGGAATCACCAG
GTGTCCCTAGAGCAGTTTTGTACTAAAGACTTCTAGTGTTTACTNCCTCACCACGGGTAC
CTTCCCAACCNNNNNNNNNNNCNCCCCNNCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NCCCCCCCCCCCCCCCCCCCCNCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCCC
CCCNCCCCCNNNCCCCCCCCCCCCNCCNCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCC
CCACCCCCNCCNACCCCCCCCCCCCCCCCCNCCCCCCNCCCCCCCCCCCCCCCCNCNCCC
CCCCCCCCNCCCCCCCCCCCCNCCCCCCNCCAACACACCCCCCCCCCCCCCACCCCCCCC
CCCACCCCCCCCCCCACCCACCCCCCACACAACCCCCACCCCCACCCACCCCACCCCCCA
CACCCCCCCCACCCCCCCACACCACCCNCCCCCCCACNCCACCACCCCCCCACCACACC
Restriction Sites NotI-NotI |
ACCN NM_006708
Insert Size 2100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006708.1, NP_006699.1
RefSeq Size 1993 bp
RefSeq ORF 555 bp
Locus ID 2739
UniProt ID Q04760
Cytogenetics 6p21.2
Domains Glyoxalase
Protein Pathways Pyruvate metabolism
Summary The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006708" in other vectors (6)
SKU Description Size Price
RC203826 GLO1 (Myc-DDK-tagged)-Human glyoxalase I (GLO1) 10 ug
$300.00
RC203826L1 Lenti ORF clone of Human glyoxalase I (GLO1), Myc-DDK-tagged 10 ug
$600.00
RC203826L2 Lenti ORF clone of Human glyoxalase I (GLO1), mGFP tagged 10 ug
$600.00
RC203826L3 Lenti ORF clone of Human glyoxalase I (GLO1), Myc-DDK-tagged 10 ug
$600.00
RC203826L4 Lenti ORF clone of Human glyoxalase I (GLO1), mGFP tagged 10 ug
$600.00
RG203826 GLO1 (tGFP-tagged) - Human glyoxalase I (GLO1) 10 ug
$489.00 $500.00

file-alt Citations

Peroxynitrite-mediated glyoxalase I epigenetic inhibition drives apoptosis in airway epithelial cells exposed to crystalline silica via a novel mechanism involving argpyrimidine-modified Hsp70, JNK, and NF-κB
Antognelli, Gambelunghe, Muzi et al
Free Radic. Biol. Med. (2015)
Cited by 36
AI Summary: OriGene’s transfection-ready plasmids pCMV-Glo1 and pCMV-GFP were used to manipulate gene expression in BEAS-2B cells, enabling the study of glyoxalase I’s role in crystalline silica-induced apoptosis.
Snippet: Italy);lipofectamine and laemmli buffer, Invitrogen (Milan, Italy);CBA, Cayman Chemical (Rome, Italy);mTempol and MnTBAP, Enzo Life Sciences (Vinci, Florence, Italy);transfection-ready plasmids pCMV-Glo1 and control DNA plasmid pCMV-GFP, OriGene (Rockville ... Cite SaveSaving
Showing 1-1 of 1 papers.
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