CALM1 (NM_006888) Human Untagged Clone

SKU SC115829

CALM1 (untagged)-Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CALM1
• Synonyms: CALML2; caM; CAM2; CAM3; CAMB; CAMC; CAMI; CAMIII; CPVT4; DD132; LQT14; PHKD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115829 sequence for NM_006888 edited (data generated by NextGen Sequencing); ATGGCTGATCAGCTGACCGAAGAACAGATTGCTGAATTCAAGGAAGCCTTCTCCCTATTT; GATAAAGATGGCGATGGCACCATCACAACAAAGGAACTTGGAACTGTCATGAGGTCACTG; GGTCAGAACCCAACAGAAGCTGAATTGCAGGATATGATCAATGAAGTGGATGCTGATGGT; AATGGCACCATTGACTTCCCCGAATTTTTGACTATGATGGCTAGAAAAATGAAAGATACA; GATAGTGAAGAAGAAATCCGTGAGGCATTCCGAGTCTTTGACAAGGATGGCAATGGTTAT; ATCAGTGCAGCAGAACTACGTCACGTCATGACAAACTTAGGAGAAAAACTAACAGATGAA; GAAGTAGATGAAATGATCAGAGAAGCAGATATTGATGGAGACGGACAAGTCAACTATGAA; GAATTCGTACAGATGATGACTGCAAAATGA; ; Clone variation with respect to NM_006888.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006888 unedited; TGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGTGGACGCCGTGCCGTTACT; CGTAGTCAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGCGCCTTAGCAGC; AGCAGCAGCAGCAGCAGCATCGGAGGTACCCCCGCCGTCGCAGCCCCCGCGCTGGTGCAG; CCACCCTCGCTCCCTCTGCTCTTCCTCCCTTCGCTCGCACCATGGCTGATCAGCTGACCG; AAGAACAGATTGCTGAATTCAAGGAAGCCTTCTCCCTATTTGATAAAGATGGCGATGGCA; CCATCACAACAAAGGAACTTGGAACTGTCATGAGGTCACTGGGTCAGAACCCAACAGAAG; CTGAATTGCAGGATATGATCAATGAAGTGGATGCTGATGGTAATGGCACCATTGACTTCC; CCGAATTTTTGACTATGATGGCTAGAAAAATGAAAGATACAGATAGTGAAGAAGAAATCC; GTGAGGCATTCCGAGTCTTTGACAAGGATGGCAATGGTTATATCAGTGCAGCAGAACTAC; GTCACGTCATGACAAACTTAGGAGAAAAACTAACAGATGAAGAAGTAGATGAAATGATCA; GAGAAGCAGATATTGATGGAGACGGACAAGTCAACTATGAAGAATTCGTACAGATGATGA; CTGCAAAATGAAGACCTACTTTCAACTCCTTTTTTCCCCCCTCTAGAAGAATCAAATTGA; ATCTTTTACTTTACCTCTTGCAAAAAAAGAAAAAGAANAAGTTCATTTATTCATTCTGTT; TCTATATAGCAAACTGATGTCAAAGTACCTTCTGTCCACAACACAAATCTGCAGNATGGG; TGGGNGGGCCCTGTCCCTAAGATCAGCTCACATCAGTTTACATATAATACTGGGACTACC; TTATGAAAAGGATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006888 unedited; GCTATGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGTTAATG; GTCTTTTTATTGGAAAAAAAAAAAAAAAGACTAGCATTTACAACTTGGAATGGACGTAAA; CTGTAATTTCTTTGAACAGCAATGTTGATGGTTGTGCTGAAGTCCACAGCCACAGCCTAC; TCTGCCGGCTCCAAGTCATGGTTCAAAAGGTTTTAAAAACAGAGAGTCCAAAGATGCTCC; CTTGGTAAATGTTTCTTATTAAAAATTTGTGTGAACAGTGACAGCCTGACACCTGTTTCA; CTCTAGAACAATGCAGACAACGCTAAACCAACATACACGGGCATGCCACCAGGGTGTGTC; ATCGTCACCATGGGTCGGAGCACACGAAGTACAAGAGGACCATCCTACCAGTGGGGCCAC; GTCTCAACGGTTTATCCTTCACCCATTTTTCTGCACGTCATCCTGAGGTAAACCAATTTT; AAATGTGTTTGTTTTCAGTAAACCAGCTATGACAATTTATACTAAACAAATTGAACTAGA; ACCCATTTGAATAGGTTTTGAATTTGTTTTTTCATTTTTCATTTTTAATACAAAGGCCTG; ACTGTGCTCAATCGTCAAGCTGCATGCATGAAAAACTGGCCAGCCCAAACAGTGTATTAA; TCATTAGCAAATGGAACTTTAAGGAGTCCTTATCATTAAGGTAGTACAAGTATTTATATT; GTAAAACTGATGTGTAGCTTGATCTTTANGGGACAGGACCACCAACCAATACATGCAGAT; TTTGTGTGTGTGGACAGAAGGTACTTTTGACATTCAGNTNTGCTTATAGAAACAGATGAT; AAATGACTTTTTTCTTTTTCTTTTTTGCAGAGGTAGTAAAGATCATTTGATTCTCTAAGC; GGAAAAGAGTGAAAGAGTCTATTTGCAGCATATCTGACAATCTCTANTGACTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006888
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006888.2, NP_008819.1
• RefSeq Size: 3718 bp
• RefSeq ORF: 450 bp
• Locus ID: 801
• UniProt ID: P62158
• Cytogenetics: 14q32.11
• Domains: EFh
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Phosphatidylinositol signaling system, Vascular smooth muscle contraction
• Summary: This gene encodes one of three calmodulin proteins which are members of the EF-hand calcium-binding protein family. Calcium-induced activation of calmodulin regulates and modulates the function of cardiac ion channels. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.A missense mutation in the CALM1 gene has been associated with ventricular tachycardia.[provided by RefSeq, May 2020]