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CALM1 (NM_006888) Human Untagged Clone

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SKU
SC115829
CALM1 (untagged)-Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1
  $150.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CALM1
Synonyms CALML2; caM; CAM2; CAM3; CAMB; CAMC; CAMI; CAMIII; CPVT4; DD132; LQT14; PHKD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115829 sequence for NM_006888 edited (data generated by NextGen Sequencing)
ATGGCTGATCAGCTGACCGAAGAACAGATTGCTGAATTCAAGGAAGCCTTCTCCCTATTT
GATAAAGATGGCGATGGCACCATCACAACAAAGGAACTTGGAACTGTCATGAGGTCACTG
GGTCAGAACCCAACAGAAGCTGAATTGCAGGATATGATCAATGAAGTGGATGCTGATGGT
AATGGCACCATTGACTTCCCCGAATTTTTGACTATGATGGCTAGAAAAATGAAAGATACA
GATAGTGAAGAAGAAATCCGTGAGGCATTCCGAGTCTTTGACAAGGATGGCAATGGTTAT
ATCAGTGCAGCAGAACTACGTCACGTCATGACAAACTTAGGAGAAAAACTAACAGATGAA
GAAGTAGATGAAATGATCAGAGAAGCAGATATTGATGGAGACGGACAAGTCAACTATGAA
GAATTCGTACAGATGATGACTGCAAAATGA

Clone variation with respect to NM_006888.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006888 unedited
TGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGTGGACGCCGTGCCGTTACT
CGTAGTCAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGCGCCTTAGCAGC
AGCAGCAGCAGCAGCAGCATCGGAGGTACCCCCGCCGTCGCAGCCCCCGCGCTGGTGCAG
CCACCCTCGCTCCCTCTGCTCTTCCTCCCTTCGCTCGCACCATGGCTGATCAGCTGACCG
AAGAACAGATTGCTGAATTCAAGGAAGCCTTCTCCCTATTTGATAAAGATGGCGATGGCA
CCATCACAACAAAGGAACTTGGAACTGTCATGAGGTCACTGGGTCAGAACCCAACAGAAG
CTGAATTGCAGGATATGATCAATGAAGTGGATGCTGATGGTAATGGCACCATTGACTTCC
CCGAATTTTTGACTATGATGGCTAGAAAAATGAAAGATACAGATAGTGAAGAAGAAATCC
GTGAGGCATTCCGAGTCTTTGACAAGGATGGCAATGGTTATATCAGTGCAGCAGAACTAC
GTCACGTCATGACAAACTTAGGAGAAAAACTAACAGATGAAGAAGTAGATGAAATGATCA
GAGAAGCAGATATTGATGGAGACGGACAAGTCAACTATGAAGAATTCGTACAGATGATGA
CTGCAAAATGAAGACCTACTTTCAACTCCTTTTTTCCCCCCTCTAGAAGAATCAAATTGA
ATCTTTTACTTTACCTCTTGCAAAAAAAGAAAAAGAANAAGTTCATTTATTCATTCTGTT
TCTATATAGCAAACTGATGTCAAAGTACCTTCTGTCCACAACACAAATCTGCAGNATGGG
TGGGNGGGCCCTGTCCCTAAGATCAGCTCACATCAGTTTACATATAATACTGGGACTACC
TTATGAAAAGGATC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006888 unedited
GCTATGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGTTAATG
GTCTTTTTATTGGAAAAAAAAAAAAAAAGACTAGCATTTACAACTTGGAATGGACGTAAA
CTGTAATTTCTTTGAACAGCAATGTTGATGGTTGTGCTGAAGTCCACAGCCACAGCCTAC
TCTGCCGGCTCCAAGTCATGGTTCAAAAGGTTTTAAAAACAGAGAGTCCAAAGATGCTCC
CTTGGTAAATGTTTCTTATTAAAAATTTGTGTGAACAGTGACAGCCTGACACCTGTTTCA
CTCTAGAACAATGCAGACAACGCTAAACCAACATACACGGGCATGCCACCAGGGTGTGTC
ATCGTCACCATGGGTCGGAGCACACGAAGTACAAGAGGACCATCCTACCAGTGGGGCCAC
GTCTCAACGGTTTATCCTTCACCCATTTTTCTGCACGTCATCCTGAGGTAAACCAATTTT
AAATGTGTTTGTTTTCAGTAAACCAGCTATGACAATTTATACTAAACAAATTGAACTAGA
ACCCATTTGAATAGGTTTTGAATTTGTTTTTTCATTTTTCATTTTTAATACAAAGGCCTG
ACTGTGCTCAATCGTCAAGCTGCATGCATGAAAAACTGGCCAGCCCAAACAGTGTATTAA
TCATTAGCAAATGGAACTTTAAGGAGTCCTTATCATTAAGGTAGTACAAGTATTTATATT
GTAAAACTGATGTGTAGCTTGATCTTTANGGGACAGGACCACCAACCAATACATGCAGAT
TTTGTGTGTGTGGACAGAAGGTACTTTTGACATTCAGNTNTGCTTATAGAAACAGATGAT
AAATGACTTTTTTCTTTTTCTTTTTTGCAGAGGTAGTAAAGATCATTTGATTCTCTAAGC
GGAAAAGAGTGAAAGAGTCTATTTGCAGCATATCTGACAATCTCTANTGACTG
Restriction Sites NotI-NotI |
ACCN NM_006888
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006888.2, NP_008819.1
RefSeq Size 3718 bp
RefSeq ORF 450 bp
Locus ID 801
UniProt ID P62158
Cytogenetics 14q32.11
Domains EFh
Protein Families Druggable Genome
Protein Pathways Alzheimer's disease, Calcium signaling pathway, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Phosphatidylinositol signaling system, Vascular smooth muscle contraction
Summary This gene encodes one of three calmodulin proteins which are members of the EF-hand calcium-binding protein family. Calcium-induced activation of calmodulin regulates and modulates the function of cardiac ion channels. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.A missense mutation in the CALM1 gene has been associated with ventricular tachycardia.provided by RefSeq, May 2020
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"NM_006888" in other vectors (6)
SKU Description Size Price
RC219086 CALM1 (Myc-DDK-tagged)-Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1 10 ug
$225.00
RC219086L1 Lenti ORF clone of Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1, Myc-DDK-tagged 10 ug
$525.00
RC219086L2 Lenti ORF clone of Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1, mGFP tagged 10 ug
$525.00
RC219086L3 Lenti ORF clone of Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1, Myc-DDK-tagged 10 ug
$525.00
RC219086L4 Lenti ORF clone of Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1, mGFP tagged 10 ug
$525.00
RG219086 CALM1 (tGFP-tagged) - Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1 10 ug
$425.00

file-alt Citations

Quantifying Intracellular Distributions of HaloTag-Labeled Proteins With SDS-PAGE and Epifluorescence Microscopy
Shangguan, Rock
Bio Protoc (2025) 15 (14), e5391 DOI: 10.21769/BioProtoc.5391
AI Summary: OriGene's Human calmodulin 1 plasmid (catalog number: SC115829) was used as a reagent in this study to support the quantification workflow for HaloTag-labeled Myo10 proteins in U2OS cells.
Snippet: ength human Myo10 sequence (nucleotide sequence from GenBank: BC137168.1), and C-terminal Flag-tag (GATTATAAAGATGATGATGATAAA). See File S1 for full nucleotide sequence 2. Human calmodulin 1 plasmid (Origene, catalog number: SC115829) 3. U2OS cells (ATCC, catalog number: HTB-96) Reagents 1. Anti-myosin10 antibody (rabbit polyclonal) (Novus, catalog number: NBP1-87748) 2. Anti-beta-tubulin anti
FigureFigureFigure
NADPH oxidase 2 activity disrupts Calmodulin/CaMKIIα complex via redox modifications of CaMKIIα-contained Cys30 and Cys289: Implications in Parkinson's disease
Pullara, Forsmann, General et al
Redox Biology (2024) 75, 103254 DOI: 10.1016/j.redox.2024.103254
Cited by 15
AI Summary: OriGene products CAMK2A transcript variant 2 (catalog SC109000) and CALM1 Human calmodulin 1 transcript variant 1 (catalog SC115829) were used as human cDNA clones to express CaMKIIα and calmodulin in Drosophila S2R+ cells for this study.
Snippet: CaMKIIα and calmodulin expression in Drosophila S2R + cells was accomplished as follows: CaMKIIα and CaM coding sequences were copied from human cDNA clones CAMK2A transcript variant 2 (catalog SC109000, Origene, Rockville MD) and CALM1 Human calmodulin 1 transcript variant 1 (catalog SC115829, Origene, Rockville MD), using PCR primers to add
Nadph Oxidase 2 Activity Disrupts Calmodulin/Camkiia Complex Via Redox Modifications of Camkiia-Contained Cys30 and Cys289: Implications in Parkinson's Disease
Di Maio, Pullara, Forsman et al
SSRN (2024)
Snippet: aM coding sequences were copied from human cDNA clones CAMK2A transcript variant 2 (catalog SC109000, Origene, Rockville MD) and CALM1 Human calmodulin 1 transcript variant 1 (catalog SC115829, Origene, Rockville MD), using PCR primers to add flanking restriction sites, with the following
Disulfide bridge formation prevents CaMKII/Calmodulin interaction in Parkinson's disease
Di Maio, General, Furbee et al
bioRxiv (2020)
Cited by 4
Snippet: and calmodulin coding sequences were copied from human cDNA clones CAMK2A transcript variant 2 (catalog SC109000, Origene, Rockville MD) and CALM1 Human calmodulin 1 transcript variant 1 (catalog SC115829, Origene, Rockville MD), using PCR primers;- and calmodulin coding sequences were copied from human cDNA clones CAMK2A transcript variant 2 (catalog SC109000, Origene, Rockville MD) and CALM1 Human calmodulin 1 transcript variant 1 (catalog SC115829, Origene, Rockville MD), using PCR primers
Long Non-coding RNA LncSHGL Recruits hnRNPA1 to Suppress Hepatic Gluconeogenesis and Lipogenesis
Wang, Yang, Chen et al
Diabetes (2018)
AI Summary: OriGene contributed to this study by providing the human CALM1 expressing plasmid (Cat No SC115829), which was used to investigate the molecular mechanisms by which LncSHGL regulates hepatic glucose and lipid metabolism.
Snippet: transfected with 5μg plasmid for 24 hours. Human CALM1-2 and hnRNPA1 expressing plasmids were purchased from Origene (CALM1, Cat No SC115829) and Vigenebio China (CALM2, Cat No CH809926;hnRNPA1, Cat No CH877838), respectively
Hepatic Activation of the FAM3C-HSF1-CaM Pathway Attenuates Hyperglycemia of Obese Diabetic Mice
Chen, Ding, Yang et al
Diabetes (2017) 66 (5), 1185-1197 DOI: 10.2337/db16-0993
Cited by 48
AI Summary: OriGene products contributed to this study by providing the FAM3C Myc-DDK-tagged overexpression plasmid (Cat# RC209651) and HSF1 Myc-DDK-tagged overexpression plasmid (Cat# RC213774), which were used to investigate the effects of FAM3C and HSF1 overexpression on hepatic glucose and lipid metabolism in obese diabetic mice and cultured hepatocytes.
Showing 1-6 of 7 papers.
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