CELF3 (NM_007185) Human Untagged Clone

CAT#: SC115671

CELF3 (untagged)-Human CUGBP, Elav-like family member 3 (CELF3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CELF3
• Synonyms: BRUNOL1; CAGH4; ERDA4; ETR-1; TNRC4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007185, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGGAGCCGGATGCCATCAAGCTGTTTGTGGGGCAGATCCCGAGGCATCTGGAGGAGAAGGACCTGA
  AGCCCATCTTCGAACAGTTTGGTCGGATCTTTGAGCTGACTGTCATCAAGGACAAGTACACCGGGCTGCA
  CAAGGGATGTGCCTTCCTGACATACTGTGCCCGGGATTCAGCCCTGAAGGCCCAGAGCGCCCTGCACGAA
  CAGAAGACGCTTCCAGGGATGAACAGGCCGATCCAGGTCAAGCCAGCCGACAGCGAGAGCCGAGGAGAAG
  ACCGGAAGCTCTTTGTGGGGATGCTAGGGAAGCAGCAGACAGATGAGGACGTCCGGAAGATGTTTGAGCC
  CTTCGGGACCATCGACGAGTGCACTGTGCTCCGGGGGCCAGATGGCACCAGCAAAGGCTGCGCCTTCGTG
  AAGTTCCAGACCCACGCTGAGGCCCAGGCGGCCATCAACACCCTTCACAGCAGCCGGACCCTGCCAGGTG
  CCTCGTCCAGCCTGGTGGTGAAGTTTGCTGACACTGAGAAGGAGCGAGGTCTCCGCCGCATGCAGCAGGT
  GGCCACCCAGTTGGGCATGTTCAGCCCCATCGCCCTCCAGTTTGGAGCCTACAGCGCCTACACCCAGGCC
  CTGATGCAGCAGCAGGCGGCCCTGGTAGCGGCTCACAGTGCCTACCTCAGCCCCATGGCCACCATGGCTG
  CCGTGCAGATGCAGCACATGGCTGCCATCAATGCCAATGGCCTCATCGCCACCCCCATCACCCCATCCTC
  AGGAACCAGCACCCCTCCTGCCATCGCTGCCACGCCTGTCTCTGCCATTCCGGCTGCCCTGGGCGTCAAC
  GGCTACAGCCCGGTGCCCACCCAGCCCACTGGGCAGCCTGCCCCTGATGCTCTGTATCCCAACGGGGTTC
  ACCCCTACCCAGCCCAGAGCCCCGCGGCCCCCGTGGACCCCCTGCAGCAGGCCTACGCGGGGATGCAGCA
  CTACACAGCAGCCTACCCAGCAGCCTACAGCCTGGTTGCACCTGCGTTCCCGCAGCCTCCAGCCCTGGTC
  GCCCAGCAGCCCCCACCACCACCTCAACAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAAA
  GAGAAGGCCCTGATGGCTGCAACATCTTCATCTACCACCTGCCCCAGGAGTTCACTGACTCAGAGATCCT
  CCAGATGTTTGTCCCCTTTGGCCACGTCATCTCAGCCAAAGTCTTTGTTGACCGAGCCACCAATCAAAGC
  AAATGTTTTGGCTTTGTGAGTTTCGACAATCCGGCCAGTGCCCAGGCTGCCATCCAGGCCATGAATGGCT
  TCCAGATCGGCATGAAGCGCCTCAAAGTCCAGCTAAAGCGGCCTAAGGATGCCAACCGGCCCTACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007185 unedited
  ACATTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGATCAAGCTGTTTG
  TGGGGCAGACCCGAGCTCTGGAGGAAAGACCTGAACCCATCTTCGAACAGTTTGGTCGGA
  TCTTTGAGCTGACTGTCATCAAGGACAAGTACACCGGGCTGCACAAGGGATGTGCCTTCC
  TGACATACTGTGCCCGGGATTCAGCCCTGAAGGCCCAGAGCGCCCTGCACGAACAGAAGA
  CGCTTCCAGGGATGAACAGGCCGATCCAGGTCAAGCCAGCCGACAGCGAGAGCCGAGGAG
  ACCGGAAGCTCTTTGTGGGGATGCTAGGGAAGCAGCAGACAGATGAGGACGTCCGGAAGA
  TGTTTGAGCCCTTCGGGACCATCGACGAGTGCACTGTGCTCCGGGGGCCAGATGGCACCA
  GCAAAGGCTGCGCCTTCGTGAAGTTCCAGACCCACGCTGAGGCCCAGGCGGCCATCAACA
  CCCTTCACAGCAGCCGGACCCTGCCAGGTGCCTCGTCCAGCCTGGTGGTGAAGTTTGCTG
  ACACTGAGAAGGAGCGAGGTCTCCGCCGCATGCAGCAGGTGGCCACCCAGTTGGGCATGT
  TCAGCCCCATCGCCCTCCAGTTTGGAGCCTACAGCGCCTACACCCAGGCCCTGATGCAGC
  AGCAGGCGGCCCTGGTAGCGGCTCACAGTGCCTACCTCAGCCNCATGGCCACCATGGNCT
  GCCGTGCAGATGCAGCACATGGCCTGCCATCAATGCCAATGGCCCTCATCGNCACCCCCA
  TCACCCCCATCCTCAGAACCAGCACCCCTTCTGCCATCGCTGNCACGCCTGTCTCTGCCA
  TTTCCGCTGCNCTTGGGCGTAAACGGCTCAGNCCCGGTGCCCACCCAGCCACTGGGCAGN
  CTGCCCCTGATGCTCTGATCAACAGGGGGTTTACCCTACCAGCCAAAGACCGCGGGCCCG
  GGAACCCCTGCAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007185 unedited
  TTGCTTTTAAACTTTTTTCTTTATTTAAACAAGAAAAGAACACAACTGGTTAAAAGTAAA
  ATGCCATAAATAAATAAGTTAAGCCTAAAGAACTGGCTGAAACTGACTCCCCGGGCTCCC
  CTTACCACCCCCACCCCCACCCCATTTTTTTTTCCCTAAAACCTCTTCCTAAAGAAAATG
  ATCAGGGAATAAACCCCTTGACCCCTTCCCACACCCCTTCTTCCAATACCCAATTACCCC
  CCAAAGCAAAGGGGGAAATTAAGAGAGAGAAAAAAATCCCAGGGCAACATTGGCTAAACT
  CCCAAAAAGAAAAAAGGCAGCGTCCGGGCCAAGCCGGGCGGGCTGGATAACCCACGGGTG
  GGAGTGGTATTTACACAGAGGGAACCAGCAGAGACTGGAAACATTTTTAGGGGGTCAGGG
  GAAGGGCTTTTCCTCGTGAAAGGAGGAGGGTCTCTGTGGCTGACTGGCAAAAAGGCAACC
  CTCAAGGCTCCCCCACACCCTCCTTAAAGGGGGCCCTTGGGGGAGGCAGTGAGTTTTAAG
  GCTGGGAGCCCAAGAATCAAGGATATCCTACCTCTAACTGAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007185
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007185.1, NP_009116.1
• RefSeq Size: 1719 bp
• RefSeq ORF: 1077 bp
• Locus ID: 11189
• UniProt ID: Q5SZQ8
• Cytogenetics: 1q21.3
• Domains: RRM
• Protein Families: Transcription Factors
• Gene Summary: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1).