SEC63 (NM_007214) Human Untagged Clone

SKU SC115637

SEC63 (untagged)-Human SEC63 homolog (S. cerevisiae) (SEC63)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SEC63
• Synonyms: DNAJC23; ERdj2; PCLD2; PRO2507; SEC63L
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007214, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCGGGCAGCAGTTCCAGTACGATGACAGTGGGAACACCTTCTTCTACTTCCTCACCTCCTTCGTGG; GGCTCATCGTGATCCCGGCGACATACTACCTCTGGCCCCGAGATCAGAATGCCGAGCAAATTCGATTAAA; GAATATCAGAAAAGTATATGGAAGGTGTATGTGGTATCGTTTACGGTTATTAAAACCCCAGCCAAATATT; ATTCCTACAGTAAAGAAAATAGTTCTGCTTGCAGGATGGGCATTGTTCTTATTCCTTGCATATAAAGTTT; CCAAAACAGACCGAGAATACCAAGAATACAATCCTTATGAAGTATTAAATTTGGATCCTGGAGCCACAGT; AGCAGAAATTAAAAAACAATATCGTTTGCTGTCACTTAAATATCATCCAGATAAAGGAGGTGATGAGGTT; ATGTTCATGAGGATAGCAAAAGCTTATGCTGCTTTAACGGATGAAGAGTCCCGGAAAAATTGGGAAGAAT; TTGGAAATCCAGATGGGCCTCAAGCCACAAGCTTTGGAATTGCCCTGCCAGCTTGGATAGTTGACCAGAA; AAACTCAATTCTGGTTTTACTTGTATATGGATTGGCATTTATGGTTATCCTTCCAGTTGTTGTGGGCTCT; TGGTGGTATCGCTCAATACGCTATAGTGGAGACCAGATTCTAATACGCACAACACAGATTTATACATACT; TTGTTTATAAAACCCGAAATATGGATATGAAACGTCTTATCATGGTTTTGGCTGGAGCTTCTGAATTTGA; TCCTCAGTATAATAAAGATGCCACAAGCAGACCAACGGATAATATTCTAATACCACAGCTAATCAGAGAA; ATTGGCAGCATTAATTTAAAGAAGAATGAGCCTCCACTTACCTGCCCATATAGCCTGAAGGCCAGAGTTC; TTTTACTGTCTCATCTTGCTAGAATGAAAATTCCTGAGACCCTTGAAGAAGATCAGCAATTCATGCTAAA; AAAGTGTCCTGCCCTACTTCAAGAAATGGTTAATGTAATCTGCCAACTAATAGTAATGGCCCGGAACCGT; GAAGAAAGGGAGTTTCGTGCTCCAACTTTGGCATCCCTAGAAAACTGCATGAAGCTTTCTCAGATGGCCG; TTCAGGGACTTCAGCAATTTAAGTCTCCCCTTCTGCAGCTCCCTCATATTGAAGAGGACAATCTTAGACG; GGTTTCTAATCATAAGAAGTATAAAATTAAAACTATCCAGGATTTGGTGAGTTTAAAAGAATCAGATCGT; CACACTCTACTGCACTTCCTTGAAGATGAAAAATATGAAGAGGTTATGGCTGTCCTTGGGAGTTTTCCAT; ATGTGACCATGGATATAAAATCACAGGTGTTAGATGATGAAGATAGCAACAACATCACAGTAGGATCCTT; AGTTACAGTGTTGGTTAAGTTGACAAGGCAAACAATGGCTGAAGTATTTGAAAAGGAGCAGTCCATCTGT; GCTGCAGAGGAACAGCCAGCAGAAGATGGGCAGGGTGAAACTAACAAGAACAGGACAAAAGGAGGATGGC; AACAGAAGAGTAAAGGACCCAAGAAAACTGCTAAATCAAAAAAAAAGAAACCTTTAAAAAAAAAACCTAC; ACCTGTGCTATTACCACAGTCAAAGCAACAGAAACAAAAGCAGGCAAATGGAGTCGTTGGGAATGAAGCT; GCAGTAAAGGAAGATGAAGAAGAAGTTTCAGATAAGGGCAGTGATTCTGAAGAAGAAGAAACCAATAGAG; ATTCCCAAAGTGAGAAAGATGATGGTAGTGACAGAGACTCTGATAGAGAGCAAGATGAAAAACAAAACAA; AGATGATGAAGCAGAGTGGCAAGAATTACAACAAAGCATACAGCGAAAAGAGAGAGCTCTATTGGAAACC; AAATCAAAAATAACACATCCTGTGTATAGCCTTTACTTTCCTGAGGAAAAACAAGAATGGTGGTGGCTTT; ACATTGCAGATAGGAAGGAGCAGACATTAATATCCATGCCATATCATGTGTGTACGCTGAAAGATACAGA; GGAGGTAGAGCTGAAGTTTCCTGCACCAGGCAAGCCTGGAAATTATCAGTATACTGTGTTTCTGAGATCA; GACTCCTATATGGGTTTGGATCAGATTAAACCATTGAAGTTGGAAGTTCATGAGGCTAAGCCTGTGCCAG; AAAATCACCCACAGTGGGATACAGCAATAGAGGGGGATGAAGACCAGGAGGACAGTGAGGGCTTTGAAGA; TAGCTTTGAGGAAGAAGAGGAGGAAGAAGAAGATGATGACTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007214 unedited; AACGTCGATTATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAGT; GCAGAGCGTGGTCGTGGCGGCGGCGGTGAGAAGAGCGAGGCGGAGGAGGGGGTGCCATGG; CCGGGCAGCAGTTCCAGTACGATGACAGTGGGAACACCTTCTTCTACTTCCTCACCTCCT; TCGTGGGGCTCATCGTGATCCCGGCGACATACTACCTCTGGCCCCGAGATCAGAATGCCG; AGCAAATTCGATTAAAGAATATCAGAAAAGTATATGGAAGGTGTATGTGGTATCGTTTAC; GGTTATTAAAACCCCAGCCAAATATTATTCCTACAGTAAAGAAAATAGTTCTGCTTGCAG; GATGGGCATTGTTCTTATTCCTTGCATATAAAGTTTCCAAAACAGACCGAGAATACCAAG; AATACAATCCTTATGAAGTATTAAATTTGGATCCTGGAGCCACAGTAGCAGAAATTAAAA; AACAATATCGTTTGCTGTCACTTAAATATCATCCAGATAAAGGAGGTGATGAGGTTATGT; TCATGAGGATAGCAAAAGCTTATGCTGCTTTAACGGATGAAGAGTCCCGGANAAATTGNG; AAGAATTTGGAAATCCAGATGGGCCTCAAGCCACAAGCTTTGGAATTGCCCTGCCAGCTT; GGATAGTTGACCAGAAAAATTCAATTCTGGTTTTACTTGTATATGGATTGGCANTTATGG; TTATCCTTCCAGTTGTTGTGGGCTCTTGGTGGTATCGCTCAATACGCTATAGTGGAGACC; AGATTCTAATACGCACAACACANGATTATACATACTTTGGTTATAAAACCCGAATATGGA; TATGAAACGTCTTATCATGGTTTTGGCTGGAGCTTCTGAATTTGATCCCTCAGTATATAA; AGATGCCACAAGCGACCACCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007214 unedited; GCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTTTTTAACGAGTCAACAAACTTC; TATTTTTATTGACAGGAGTCCAAAATGAATACAAAAAAAGCTTCCTTTGCATGTTATACT; TACTTTCCTTAAAAAGCTCCTGATAGAGTCAAGTAGACTGAAAACTAAGGATGTAGTTCA; GCTTTTAGAGTGAACCTTTTTTAAAAAAGGTTTAATAACATAAGCCACCAGTATCAGAAA; ACATACAGTCCCACTATCACTTTTAGAGCTAGAACTTATCAAAAGAATTAGATCCTGCAT; TGATCTGCTAAGATAGAGGTTTAGAAATGGTTTAAGCCATTAAAAGCCATTTTTATAATC; TGTCTGATAACTCTGGGCAATTAAGAAAAGGAAAATTCCAAAACGTAGCCCTCTATATCA; TGTGGAATGTGAGAGAATGCCAACTTATGACCAAGATAAGTAAAAATAAAAGTTTAAATC; TATGAGTGGAGCTGTTGTATCACTGCAAAATGTCAACAACAGAGCTTATCAAGAGATTAT; CAACTTGAGCGATGTTACTTAAACTTGAGCGATGTTACTTAAAACATATTTGAGGAGTTT; GCTAAAACTAAAGCTGAACAAAGAAAAGTCGCTCATTTACAGACTGTGTTTCCTGCATCT; TTACTTTTACTGGGACCATAGACTGATNCAGATATACATAGCCCACACTTCTGGACAGTT; ANTATAACAAAGGATTTATTATCATTTGCAGATGAAATAAATGTACCATCCCCTACTTGA; AAGGTTTCAATAAGCCTTACATTCTTCAGGTTGTACCAAGCACCGCACTGNCTAGTTATA; TATGCACCATTNCTAGATANAAAACTACCCTNCCTCAAATCAGTTCTGTACTGTTNCTGG; TNATGATACACTTCATACAGCAAAAATGCAATTGTGCAACACTGGGNNCATTCAGAGACT; GCTAGNCTCATCTCTCTCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_007214
• Insert Size: 3240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007214.3, NP_009145.1
• RefSeq Size: 3850 bp
• RefSeq ORF: 2283 bp
• Locus ID: 11231
• UniProt ID: Q9UGP8
• Cytogenetics: 6q21
• Domains: DnaJ, Sec63
• Protein Families: Transmembrane
• Summary: The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq, Jul 2008]