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PHLDA1 (NM_007350) Human Untagged Clone

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SKU
SC115552
PHLDA1 (untagged)-Human pleckstrin homology-like domain, family A, member 1 (PHLDA1)
  $450.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PHLDA1
Synonyms DT1P1B11; PHRIP; TDAG51
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115552 sequence for NM_007350 edited (data generated by NextGen Sequencing)
ATGAGGCGTGCGCCGGCTGCCGAGCGCCTCTTGGAGCTGGGCTTTCCCCCGCGGTGCGGG
CGCCAGGAGCCGCCTTTTCCGCTGGGTGTCACTCGGGGGTGGGGAAGATGGCCCATTCAA
AAGCGCCGCGAGGGGGCCCGGCCAGTGCCCTTCAGTGAGCGCTCGCAAGAGGACGGCAGA
GGCCCGGCAGCTCGGAGCTCCGGGACCTTGTGGCGCATCAGGACGCGGCTGTCCCTCTGC
CGGGACCCAGAGCCGCCGCCGCCGCTCTGCCTCCTGCGTGTTAGCCTCCTCTGCGCGCTC
CGGGCAGGCGGCCGTGGGAGCCGCTGGGGCGAGGACGGCGCGAGGCTGCTGCTGCTGCCC
CCGGCCCGCGCGGCTGGAAACGGAGAGGCCGAGCCAAGCGGCGGCCCCTCTTATGCTGGG
AGGATGCTGGAGAGTAGCGGCTGCAAAGCGCTGAAGGAGGGCGTGCTGGAGAAGCGCAGC
GACGGGTTGTTGCAGCTCTGGAAGAAAAAGTGTTGCATCCTCACCGAGGAAGGGCTGCTG
CTTATCCCGCCCAAGCAGCTGCAACACCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAG
CAGCAACAACAGCCCGGGCAGGGGCCGGCCGAGCCGTCCCAACCCAGTGGCCCCGCTGTC
GCCAGCCTCGAGCCGCCGGTCAAGCTCAAGGAACTGCACTTCTCCAACATGAAGACCGTG
GACTGTGTGGAGCGCAAGGGCAAGTACATGTACTTCACTGTGGTGATGGCAGAGGGCAAG
GAGATCGACTTTCGGTGCCCGCAAGACCAGGGCTGGAACGCCGAGATCACGCTGCAGATG
GTGCAGTACAAGAATCGTCAGGCCATCCTGGCGGTCAAATCCACGCGGCAGAAGCAGCAG
CACCTGGTCCAGCAGCAGCCCCCCTCGCAGCCGCAGCCGCAGCCGCAGCTCCAGCCCCAA
CCCCAGCCTCAGCCTCAGCCGCAACCCCAGCCCCAATCACAACCCCAGCCTCAGCCCCAA
CCCAAGCCTCAGCCCCAGCAGCTCCACCCGTATCCGCATCCACATCCACATCCACACTTT
CATCCTCACTCGCACCCACACCCTCACCCGCACCCGCATCCGCACCAAATACCGCACCCA
CACCCACAGCCGCACTCGCAGCCGCACGGGCACCGGCTTCTCCGCAGCACCTCCAACTCT
GCCTGA

Clone variation with respect to NM_007350.3
1079 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_007350 unedited
CGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGTGGACGCAG
CGGGCTTTGGAAAGGCCCCAAGTTAATGAGGCGTGCGCCGGCTGCCGAGCGCCTCTTGGA
GCTGGGCTTTCCCCCGCGGTGCGGGCGCCAGGAGCCGCCTTTTCCGCTGGGTGTCACTCG
GGGGTGGGGAAGATGGCCCATTCAAAAGCGCCGCGAGGGGGCCCGGCCAGTGCCCTTCAG
TGAGCGCTCGCAAGAGGACGGCAGAGGCCCGGCAGCTCGGAGCTCCGGGACCTTGTGGCG
CATCAGGACGCGGCTGTCCCTCTGCCGGGACCCAGAGCCGCCGCCGCCGCTCTGCCTCCT
GCGTGTTAGCCTCCTCTGCGCGCTCCGGGCAGGCGGCGTNGGGAGCCCGCTGGGGGCGAG
GGACCGCGCGAGGCTGCTGCTGCTGCCCCCGGCCCGCGCGGCTGGAAACGGAGAGGCCGA
GCCAAGCGGCGGCCCCTCTTATGCTGGGAGGATGCTGGAGAGTAGCGGCTGCAAAGCGCT
GAAGGAGGGCGTGCTGGAGAAGCGCAGCGACGGGTTGTTGCAGCTCTGGAAGAAAAAGTG
TTGCATCCTCACCGAGGAAGGGCTGCTGCTTATCCCGCCCAAGCAGCTGCAACACCAGCA
GCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAACAGCCCGGNCCAAGGGGCCCGGCCGA
GCCGTCCCAACCCAGTGGCCCCGCTGTCGCCAGCCTCGAGCCGCCGGTCAAGCTCAAGGA
ACTGCACTTCTCCACATGAAGACCGTGGACTGGTGTGGAGCGCAGGGCAAGTACATGTAC
TTCACTGTGGGTGATGGCAGAGGCAAAGGAGATCGACTTTCGGTGCCCGCAGACCCAGGC
TGGAACGCCGAGATCCGCTGCAATGGGTGCAGTACA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_007350 unedited
TCTGGCACGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTTTAATATCAGA
GTTTTAATTTCAACCAGCTGGCACAACAATGAAAGTGTCAGACTTTCTGAAGTACTCGAG
AAATAATGAATAAATTCTTAATGTTTTCCCCTCCACCGCCCTTTTTTATTCTCCAAGATT
AGGAATTACTACGGATTAGGTTTTTGAAAATAAAGTTTCCTTTTTGGAAAATGGTCTACA
TTCAGAAATGTCTTAGAACAAGCATTTAAAAAAAACTAATAAATAATCATAAATCAAAAT
ACATTAAAATAAAATTACAGTACATCATCGCTCCTAGAAAATTCACCATACAAGACGATC
CTTTCAAAGGTTCATAAATAAAAGTCTTCTTGACTCGAAATCGTTTCCTGCATCGTGATG
AAAAGTATGCAGAAAACTAAGAAGAATCGCAAGTTTTCAGTAGGGTGATGTCCAAACTAC
TTGATCTGGTGCGGGGCGGAGAGACTGTTTTGCTTTTGATCCAAGTGAAGACAATAGAAA
TGTGCTCGTCCCACTTCCTCAAGTCCTCAAAACCTTGTCTTGCCCGGGAGCTGCCCCTTT
CAGGCAGAGTTGGAGGTGCTGCGGAGAATCCGGTGCCCGTGCGGCTGCGATTGCGGCTGT
GGGTGTGGGTGCGGTATTTGGTGCGGATGCGGGTGCGGGTGATGGGTGTGGGTGCAATTG
AGGATGAAGGNTNGGTNTTGGNATTTGGGNTTCCCGTTTCCGGTTGGGACTGCTGGGGCT
GAAGCTTGGGTTGGGGCTGGGGCCTGGGTTGTGATTGGGGCTGGGGTTCCGGCTGAGGCT
GGGGCTGGGTTGGGGCTGGTCCTTCGCTTTCGCTTCGCTCGCAGGGGGCCGCTCCTGACA
CTTGCCTTGGTTTTGCCCGGGGTTTTCCGCCAGAAGCCCCCCATTCTTGACTGACACTTG
AACGGATCCGGGTTCACCCG
Restriction Sites NotI-NotI |
ACCN NM_007350
Insert Size 1800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007350.2, NP_031376.2
RefSeq Size 1998 bp
RefSeq ORF 780 bp
Locus ID 22822
UniProt ID Q8WV24
Cytogenetics 12q21.2
Domains PH
Summary This gene encodes an evolutionarily conserved proline-histidine rich nuclear protein. The encoded protein may play an important role in the anti-apoptotic effects of insulin-like growth factor-1. provided by RefSeq, Jul 2008
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"NM_007350" in other vectors (7)
SKU Description Size Price
RC220155 PHLDA1 (Myc-DDK-tagged)-Human pleckstrin homology-like domain, family A, member 1 (PHLDA1) 10 ug
$686.00
RC220155L1 Lenti ORF clone of Human pleckstrin homology-like domain, family A, member 1 (PHLDA1), Myc-DDK-tagged 10 ug
$986.00
RC220155L2 Lenti ORF clone of Human pleckstrin homology-like domain, family A, member 1 (PHLDA1), mGFP tagged 10 ug
$986.00
RC220155L3 Lenti ORF clone of Human pleckstrin homology-like domain, family A, member 1 (PHLDA1), Myc-DDK-tagged 10 ug
$986.00
RC220155L4 Lenti ORF clone of Human pleckstrin homology-like domain, family A, member 1 (PHLDA1), mGFP tagged 10 ug
$986.00
RG220155 PHLDA1 (tGFP-tagged) - Human pleckstrin homology-like domain, family A, member 1 (PHLDA1) 10 ug
$886.00
SC317635 PHLDA1 (untagged)-Human pleckstrin homology-like domain, family A, member 1 (PHLDA1) 10 ug
$732.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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