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SPON2 (NM_012445) Human Untagged Clone

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SKU
SC115352
SPON2 (untagged)-Human spondin 2, extracellular matrix protein (SPON2), transcript variant 1
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SPON2
Synonyms DIL-1; DIL1; M-SPONDIN; MINDIN
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115352 sequence for NM_012445 edited (data generated by NextGen Sequencing)
ATGGAAAACCCCAGCCCGGCCGCCGCCCTGGGCAAGGCCCTCTGCGCTCTCCTCCTGGCC
ACTCTCGGCGCCGCCGGCCAGCCTCTTGGGGGAGAGTCCATCTGTTCCGCCAGAGCCCCG
GCCAAATACAGCATCACCTTCACGGGCAAGTGGAGCCAGACGGCCTTCCCCAAGCAGTAC
CCCCTGTTCCGCCCCCCTGCGCAGTGGTCTTCGCTGCTGGGGGCCGCGCATAGCTCCGAC
TACAGCATGTGGAGGAAGAACCAGTACGTCAGTAACGGGCTGCGCGACTTTGCGGAGCGC
GGCGAGGCCTGGGCGCTGATGAAGGAGATCGAGGCGGCGGGGGAGGCGCTGCAGAGCGTG
CACGAGGTGTTTTCGGCGCCCGCCGTCCCCAGCGGCACCGGGCAGACGTCGGCGGAGCTG
GAGGTGCAGCGCAGGCACTCGCTGGTCTCGTTTGTGGTGCGCATCGTGCCCAGCCCCGAC
TGGTTCGTGGGCGTGGACAGCCTGGACCTGTGCGACGGGGACCGTTGGCGGGAACAGGCG
GCGCTGGACCTGTACCCCTACGACGCCGGGACGGACAGCGGCTTCACCTTCTCCTCCCCC
AACTTCGCCACCATCCCGCAGGACACGGTGACCGAGATAACGTCCTCCTCTCCCAGCCAC
CCGGCCAACTCCTTCTACTACCCGCGGCTGAAGGCCCTGCCTCCCATCGCCAGGGTGACA
CTGCTGCGGCTGCGACAGAGCCCCAGGGCCTTCATCCCTCCCGCCCCAGTCCTGCCCAGC
AGGGACAATGAGATTGTAGACAGCGCCTCAGTTCCAGAAACGCCGCTGGACTGCGAGGTC
TCCCTGTGGTCGTCCTGGGGACTGTGCGGAGGCCACTGTGGGAGGCTCGGGACCAAGAGC
AGGACTCGCTACGTCCGGGTCCAGCCCGCCAACAACGGGAGCCCCTGCCCCGAGCTCGAA
GAAGAGGCTGAGTGCGTCCCTGATAACTGCGTCTAA

Clone variation with respect to NM_012445.3
365 c=>a;724 g=>c
5' Read Nucleotide Sequence
>OriGene 5' read for NM_012445 unedited
NGGTGTCGGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATCGGCACCANAGAGGTG
ATCCGACCCGGGGAAGGTCGCTGGGCAGGGCGAGTTGGGAAAGCGGCAGCCCCCGCCGCC
CCCGCAGCCCCTTCTCCTCCTTTCTCCCACGTCCTATCTGCCTCTCGCTGGAGGCCAGGC
CGTGCAGCATCGAAGACAGGAGGAACTGGAGCCTCATTGGCCGGCCCGGGGCGCCGGCCT
CGGGCTTAAATAGGAGCTCCGGGCTCTGGCTGGGACCCGACCGCTGCCGGCCGCGCTCCC
GCTGCTCCTGCCGGGTGATGGAAAACCCCAGCCCGGCCGCCGCCCTGGGCAAGGCCCTCT
GCGCTCTCCTCCTGGCCACTCTCGGCGCCGCCGGCCAGCCTCTTGGGGGAGAGTCCATCT
GTTCCGCCAGAGCCCCGGCCAAATACAGCATCACCTTCACGGGCAAGTGGAGCCAGACGG
CCTTCCCCAAGCAGTACCCCCTGTTCCGCCCCCCTGCGCAGTGGTCTTCGCTGCTGGGGG
CCGCGCATAGCTCCGACTACAGCATGTGGAGGAAGAACCAGTACGTCAGTAACGGGCTGC
GCGACTTTGCGGAGCGCGGCGAGGCCTGNGCGCTGATGAAGGAGATCGAGGCGGCGGNGG
AGGCGCTGCANAGCGTGCACGAGGTGTTTTCGGCGCCCGCCGTCCCCAGCGGCACCGGGC
AGACGTCGGCGGAGCTGGAGGTGCAGCGCANGCACTCGCTGGTCTCGTTTGTGTGCGCAT
CGTGCCCAGCCCCGACTGGTTCGTGGGCGTGGACAGCCTGGACCTGTGCGACGNNGNACC
GTTGCGGGAACAGGNCGNGCTGNACCTGTACCCCTACGACGCCGGNACGGACAAGCGCTT
CACCTTCTCCTCCCCAN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_012445 unedited
GTACAAAACGCTTTTAGGTCGAGTTAAAATTCTTTTTTTTTGTGAGCAACAGAGAAGTCT
TTATTCAAACGCAGGAAAGATCCATAACATGGAAACACTGACGCTTCCGAAACCGCCCCA
TTTATTCACTTCTCAAGTGGCCCCCGCTTGGATGCGCCCTCGGGAGAGTGGGCTCAGCAC
AGCCTAGAGCACCAGGTCTGAGGTTNCCTCAATCACGTGGGAGCCAGGCCCCTGGACGAT
GAAGGACAATCTCCTGGAGCAGCAATAACTTATAAGGAGACATAATTTAGAGTAGCTGGA
GCCTTGGGGATGACTTTATCCTGCAGGAGGAGGAGGCTGAGAGCAGACGGGACACGGGGG
CCCCTAAGAAGCAAGGTTGGGAAAGGAGGAGGCTGTTTCCCAATGCCCGTGCCGGCCACC
AGAGGGCCCTTCAGTGCAGAGATGGTCGGCGCGGCCTCACCGCGGTCAGGAGCAGCGCGA
AACCCCCTGTGCCCTCGGCCGCCTGCAGCATGAGCCTGCACAGGAGCCCCCGACACCCCA
TGGCTCCGGGGGGCCCCAGGGGCTGCGGGGCTCTGGTCTTAGACGCAGTTATCAGGGACG
CACTCAGCCTCTTCTTCGAGCTCGGGGCAGGGGCTCCCGTTGTTGGCGGGCTGGACCCGG
ACGTAACGAGTCCTGCTCTTGTCCCGAGCCTCCACAGTGGGCTCCGCCAGTCCCCAGACG
ACCACAGGAGACCTCGCATCCAGCGGGGTTTCTGAACTGAGGGCTGGCTAAATCTATTGG
CCCTGCTGGCAGGACTGGGGGGCAGGGATGAAGGCCTGGGGCTTTGCCCCGCCCAACATG
TCCCCCTGTCATGGAGGCGGGCCTTCCCCCCGGGTATTATAAGAATGGCCGCCGGCTGGG
AAAGGAGGCTTTTTTTGCCCCCGGTCTCGCCGGAGTGGCCAACTGGGCGGCAATCCAAAC
TCTCTTTA
Restriction Sites NotI-NotI |
ACCN NM_012445
Insert Size 1990 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012445.1, NP_036577.1
RefSeq Size 1807 bp
RefSeq ORF 996 bp
Locus ID 10417
UniProt ID Q9BUD6
Cytogenetics 4p16.3
Domains tsp_1
Protein Families Secreted Protein
Summary Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3.
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Documents
Resources
"NM_012445" in other vectors (7)
SKU Description Size Price
RC206764 SPON2 (Myc-DDK-tagged)-Human spondin 2, extracellular matrix protein (SPON2), transcript variant 1 10 ug
$300.00
RC206764L1 Lenti ORF clone of Human spondin 2, extracellular matrix protein (SPON2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC206764L2 Lenti ORF clone of Human spondin 2, extracellular matrix protein (SPON2), transcript variant 1, mGFP tagged 10 ug
$600.00
RC206764L3 Lenti ORF clone of Human spondin 2, extracellular matrix protein (SPON2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC206764L4 Lenti ORF clone of Human spondin 2, extracellular matrix protein (SPON2), transcript variant 1, mGFP tagged 10 ug
$600.00
RG206764 SPON2 (tGFP-tagged) - Human spondin 2, extracellular matrix protein (SPON2), transcript variant 1 10 ug
$489.00 $500.00
SC322595 SPON2 (untagged)-Human spondin 2, extracellular matrix protein (SPON2), transcript variant 1 10 ug
$300.00

file-alt Citations

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