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MKRN1 (NM_013446) Human Untagged Clone

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SKU
SC115218
MKRN1 (untagged)-Human makorin ring finger protein 1 (MKRN1), transcript variant 1
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MKRN1
Synonyms RNF61
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_013446 edited
GAATTCGGCACGAGGGTAATGGCGGAGGCTGCAACTCCCGGAACAACAGCCACAACATCA
GGAGCAGGAGCGGCAGCGGCGACGGCGGCAGCAGCCTCCCCCACCCCGATCCCCACAGTC
ACCGCCCCGTCCCTGGGGGCGGGCGGAGGGGGCGGCGGCAGCGACGGCAGCGGCGGCGGC
TGGACTAAACAGGTCACCTGCAGGTATTTTATGCATGGGGTTTGTAAGGAAGGAGACAAC
TGTCGCTACTCGCATGACCTCTCTGACAGTCCGTATAGTGTAGTGTGCAAGTATTTTCAG
CGAGGGTACTGTATTTATGGAGACCGCTGCAGATATGAACATAGCAAACCATTGAAACAG
GAAGAAGCAACTGCTACAGAGCTAACTACAAAGTCATCCCTTGCTGCTTCCTCAAGTCTC
TCATCGATAGTTGGACCACTTGTTGAAATGAATACAGGCGAAGCTGAGTCAAGAAATTCA
AACTTTGCAACTGTAGGAGCAGGTTCAGAGGACTGGGTGAATGCTATTGAGTTTGTTCCT
GGGCAACCCTACTGTGGCCGTACTGCGCCTTCCTGCACTGAAGCACCCCTGCAGGGCTCA
GTGACCAAGGAAGAATCAGAGAAAGAGCAAACCGCCGTGGAGACAAAGAAGCAGCTGTGC
CCCTATGCTGCAGTGGGAGAGTGCCGATACGGGGAGAACTGTGTGTATCTCCACGGAGAT
TCTTGTGACATGTGTGGGCTGCAGCTCCTGCATCCAATGGATGCTGCCCAGAGATCGCAG
CATATCAAATCGTGCATTGAGGCCCATGAGAAGGACATGGAGCTCTCATTTGCCGTGCAG
CGCAGCAAGGACATGGTGTGTGGGATCTGCATGGAGGTGGTCTATGAGAAAGCCAACCCC
AGTGAGCGCCGCTTCGGGATCCTCTCCAACTGCAACCACACCTACTGTCTCAAGTGCATT
CGCAAGTGGAGGAGTGCTAAGCAATTTGAGAGCAAGATCATAAAGTCCTGCCCAGAATGC
CGGATCACATCTAACTTTGTCATTCCAAGTGAGTACTGGGTGGAGGAGAAAGAAGAGAAG
CAGAAACTCATTCTGAAATACAAGGAGGCAATGAGCAACAAGGCGTGCAGGTATTTTGAT
GAAGGACGTGGGAGCTGCCCATTTGGAGGGAACTGTTTTTACAAGCATGCGTACCCTGAT
GGCCGTAGAGAGGAGCCACAGAGACAGAAAGTGGGAACATCAAGCAGATACCGGGCCCAA
CGAAGGAACCACTTCTGGGAACTCATTGAGGAAAGAGAGAACAGCAACCCCTTTGACAAC
GATGAAGAAGAGGTTGTCACCTTTGAGCTGGGCGAGATGTTGCTTATGCTTTTGGCTGCA
GGTGGGGACGACGAACTAACAGACTCTGAAGATGAGTGGGACTTGTTTCATGATGAGCTG
GAAGATTTTTATGACTTGGATCTATAGCAACCTTGCGTGGCGTGTGAACTGGTCTGCTGA
CCTCAGACAGCAGCTGTCCCCTGTGGTGGTGTGGCAGTGCCTGTGTTCTCTCCTAGGCAG
GCCTCTCAACTCCAGGTGCTGTCCTAAGAATTTTTACCCAGGGCCTGTCTTCTCAACCCC
TCACCTTTCCCTGAGGAGTGTGTTGTTTTCCCTGTTGAAAAAAGTTACAAAAATAAATCT
TAAAGTTAGTTTTTTGTAACACGAATTTAACTGTCAGACAGTTAGTGTAGGTGTGTTGCG
TCATCTGTTTTCAACCAGATTGCATTTATGGACTTTTCACACACTCATTTTGAGGACCCC
AGGTTCAAAAGTAAAAGCAGTGGCCCTGCTTTGGGGTCCAAGAATAGGAGTGATGGGTGA
AGGGACCTAAGCTGGCCAATAGCCCTCTGCCCCAGACATGGGATGTGGATCCTTGAGGTT
TCTGGTGAAATCTGCACATCTGTGTTTTTATATCTGTTCCCTACCCTGTAATCCCTACCA
CGTGCACTTGTTCTGTGGTTTTGGTCTCTTGTTTAATTGCACACAAGTAATACTACTGGG
TAACCAGAATCAGGTGTGAATGTGTTGAGATTTTTTACTGTTTTGCATGATAGGAAAATT
GAGAAAGAATACGTATAAAAGATAGAGAGGCATAACATCAATGCAGAGTTGGAAGTTGGC
TCCCAAGGGCTGACATGGTGTGAGTGTGTGGGTGTGTGATAAGCTTCTCATCCCTGCATA
GATGCAGTATTCTTAGCCTTAGTAGAAAAACCTGGTTTAGTGGTTTAAGCCTTGTGTGGC
AGATAGATCTTAAAGGGCAAAGCAGTATATTGGTAGTTGTCAATATAGCAGTGCTAGCTC
TGTCTATATAAATAGAGAAATGGGGTTAGCCATAGAGGTTAAAACTACCTGGTTATCCCA
TATAATAACACAAACTGGGTCTTGGATACACAGTTGTATTTAATGTTTTACGATCTAGCC
TTTCCAGTACAGGCACTTTCTGAGAAACCTTTGTCCTCACTTGAGGCATTTTGTTGTCGG
GTTTTTGTGTTTGTTTTTGTGGGTATTTGCCTCATTCCACCCCTGAGCTTTCAGGTAGAC
AGACGTGATTCAAAACTCTGTTCTAAGGTGTTTATTGTAGTGGAGTAATGGGTTTGCAGT
GATAAGTCATACTTTTCCACCGAAAGGGAGGGCTTGGGAATCCCTGAGATTAGCTAAAGT
TAAGTTGTTGGAAGAATTCCTTGATTGGAAATTGTACCTTTGTGTTTTGTTGCTCTGTTT
CCTGAAAATAACTCGGGGATGCTCCTGGTTTGTCCATCTACTGCTTTGATTCCTTGGATC
CCACCCATTCTTTCACTTTAAGAAAAAACAAATAATTGTTGCAGAGGTCTCTGTATTTTG
CAGCTGCCCTTTTGTAAGAAGCACTTTTCCCAAATAAAACAATTAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_013446 unedited
GGGTTTAAATTTGTAAACGACTCATATAGGCGGCCCGCGAATCGGCAGGAGGGTAATGGC
GGAGGCTGCAACTCCCGGNACAACAGCCACAACATCAGGAGCAGGAGCGGCAGCGGCGAC
GGCGGCAGCAGCCTCCCCCACCCCGATCCCCACAGTCACCGCCCCGTCCCTGGGGGCGGG
CGGAGGGGGCGGCGGCAGCGACGGCAGCGGCGGCGGCTGGACTAAACAGGTCACCTGCAG
GTATTTTATGCATGGGGTTTGTAAGGAAGGAGACAACTGTCGCTACTCGCATGACCTCTC
TGACAGTCCGTATAGTGTAGTGTGCAAGTATTTTCAGCGAGGGTACTGTATTTATGGAGA
CCGCTGCAGATATGAACATAGCAAACCATTGAAACAGGAAGAAGCAACTGCTACAGAGCT
AACTACAAAGTCATCCCTTGCTGCTTCCTCAAGTCTCTCATCGATAGTTGGACCACTTGT
TGAAATGAATACAGGCGAAGCCTGAGTCAAGAAATTCAAACTTTGCAACTGTAGGAGCAG
GTTCAGAGGACTGGGTGAATGCCTATTGAGTTTGTTCCTGTGCAACCCTACTGTGGCCGT
ACTGCGCCTTCCTGCACTGAAGCACCCCTGCAGGGCCTCACTGACCACAGGAAGAATCAG
AGAAAGAGCAAACCCGCCGCTGGAGACCAAAGAACCAGCTGTGCCCCCTCATGCTGCAGT
GGGGAGAGTGCCCGATACCCGGGAGAACTGTGTGCATCTCCACGGCAGATTCTTGTGAAC
ATGTGTGGGGCTGCAGGTCCTGCATTCCAAGGGATGCTGCCCCAAAGACCGCAGCATCAT
CAAATCCTGCATTGAAGCCCCATGAGAAAGACATGAAGCCCTCATTTGCCCGGGCACAGC
ACCAAGCACAGGGA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_013446 unedited
CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTAATCGTTTTATTTGGGAAAAAGTGCTTCTTACAAAAGGGCAGCTGCAAAATACAAA
AACCTCTGCAACAATTATTTGTTTTTTCTTAAAGGGAAAAAATGGGTGGGATCCAAGGAA
TCAAAGCAGTAGATGGACAAACCAGGAGCCTCCCCGAGTTATTTTCAGGAAACAGACCAA
CAAAACACAAAGGTCCAATTCCCAATCAAGGAATTCTTCCAACAACTTAACTTTATCTAA
TCTCAGGGATTCCCAACCCCTCCCTTTCGGTGGAAAAGTATGACTTATCACTGCAAACCC
ATTACTCCACTACAATAAACACCTTAAAACAAAGCTTTGAATCACGTCTGTCTACCTGAA
CGCCTAGGGGCGGCATGAGGCAAATCCCCACCAAACCAAACACAAAAACCCCACCACAAA
ATGCCTTAAGCGAGGACAAAGGTTTCCCAAAAAGTGCCTGTCCTGCAAAGGCCTACACCC
CAAAACCTTAAACACAACTGGGTTTTCAAAACCCCATTTGTGCTATCATATGGGACAACC
CAGTCACTCTAACCTTTATGGTTCACCCCCATTTGTCTCTTTCCTATAAACAAACCTACC
TACTGTCTATTTGCAAATTAACAACCTACTGGCTCGCCCTTTAAAATCCTCCTGCCACAC
AGGCTTAACTTCTTACACCGTCCTTTTTCTAAGGCCAAAATCTGCCTCCTCTCGGGATGA
AAACTTACTAACACCCCCCATTTCTCCTTGCCCTCCTTGGAGACACTTCCACTTTCATTG
CGCCCTGCCCCTTCCCTTTACCCCTTCTTTTCCCTCTCCTTCGCACAAACTTAAATTGTA
CTTTCCCCCCGTTTGGCCCCCCCCCTTACTTGCGCCCCCAACCTAACCCCCCG
Restriction Sites NotI-NotI |
ACCN NM_013446
Insert Size 3070 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013446.2, NP_038474.1
RefSeq Size 3116 bp
RefSeq ORF 1449 bp
Locus ID 23608
UniProt ID Q9UHC7
Cytogenetics 7q34
Domains RING, zf-CCCH
Protein Families Druggable Genome
Summary This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Apr 2014
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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"NM_013446" in other vectors (6)
SKU Description Size Price
RC206848 MKRN1 (Myc-DDK-tagged)-Human makorin ring finger protein 1 (MKRN1), transcript variant 1 10 ug
$457.00
RC206848L1 Lenti ORF clone of Human makorin ring finger protein 1 (MKRN1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC206848L2 Lenti ORF clone of Human makorin ring finger protein 1 (MKRN1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC206848L3 Lenti ORF clone of Human makorin ring finger protein 1 (MKRN1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC206848L4 Lenti ORF clone of Human makorin ring finger protein 1 (MKRN1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG206848 MKRN1 (tGFP-tagged) - Human makorin ring finger protein 1 (MKRN1), transcript variant 1 10 ug
$489.00 $657.00

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