DIMT1L (DIMT1) (NM_014473) Human Untagged Clone

SKU SC114978

DIMT1 (untagged)-Human DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) (DIMT1L)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DIMT1L
• Synonyms: DIM1; DIMT1L; HSA9761; HUSSY5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114978 sequence for NM_014473 edited (data generated by NextGen Sequencing); ATGCCGAAGGTCAAGTCGGGGGCCATCGGCCGCCGCCGCGGGCGGCAGGAGCAGCGCCGG; GAGCTGAAGAGCGCTGGAGGACTCATGTTCAACACGGGGATTGGGCAGCACATTTTGAAA; AATCCTCTCATTATTAACAGCATTATCGATAAGGCTGCCTTAAGACCAACTGATGTAGTG; CTGGAAGTTGGACCTGGAACTGGCAACATGACTGTAAAGTTGTTAGAAAAGGCAAAAAAG; GTTGTTGCTTGTGAACTTGACCCAAGGCTAGTAGCTGAACTTCACAAAAGAGTTCAGGGC; ACGCCTGTGGCCAGCAAACTTCAAGTACTGGTGGGTGATGTGCTGAAAACAGATTTGCCA; TTCTTTGATACTTGTGTGGCAAATTTGCCTTATCAGATCTCTTCACCTTTTGTCTTCAAG; CTGTTGCTACATCGACCTTTTTTCAGGTGTGCTATACTTATGTTTCAAAGAGAATTTGCC; CTCCGACTGGTTGCAAAACCTGGAGATAAGTTATACTGCAGACTCTCAATTAATACACAG; CTGTTGGCACGTGTGGACCATCTAATGAAAGTGGGAAAGAATAACTTCAGACCACCGCCC; AAGGTGGAATCCAGTGTTGTAAGGATAGAACCTAAGAATCCACCACCACCCATCAATTTT; CAGGAATGGGATGGTCTAGTAAGGATAACCTTTGTTAGGAAAAACAAGACACTCTCTGCT; GCATTTAAATCAAGTGCAGTGCAACAACTCTTGGAAAAAAACTACAGAATTCACTGTTCA; GTCCATAATATTATAATACCAGAAGATTTCAGCATAGCAGATAAAATACAGCAAATCCTA; ACCAGCACAGGTTTTAGTGACAAACGGGCCCGTTCCATGGACATAGATGACTTCATCAGA; TTGCTACATGGATTCAACGCAGAAGGTATTCATTTTTCCTAG; ; Clone variation with respect to NM_014473.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014473 unedited; GGTGTACATATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCTCG; TGCCGAATTCGGCACGAGGGGCGCTTGGGCCAGAGTGGTGCGGGCGGCGCGTGGCGGCGA; TCCCCACGTGGTGGCTTTCTCACGCTAGCCCTCTTTGGTCCTCCTTGACGTCCCGGGCCT; GTGGGCCCGCTTTCTGCCGAGATGCCGAAGGTCAAGTCGGGGGCCATCGGCCGCCGCCGC; GGGCGGCAGGAGCAGCGCCGGGAGCTGAAGAGCGCTGGAGGACTCATGTTCAACACGGGG; ATTGGGCAGCACATTTTGAAAAATCCTCTCATTATTAACAGCATTATCGATAAGGCTGCC; TTAAGACCAACTGATGTAGTGCTGGAAGTTGGACCTGGAACTGGCAACATGACTGTAAAG; TTGTTAGAAAAGGCAAAAAAGGTTGTTGCTTGTGAACTTGACCCAAGGCTAGTAGCTGAA; CTTCACAAAAGAGTTCAGGGCACGCCTGTGGCCAGCAAACTTCAAGTACTGGTGGGTGAT; GTGCTGAAAACAGATTTGCCATTCTTTGATACTTGTGTGGCAAATTTGCCTTATCAGATC; TCTTCACCTTTTGTCTTCAAGCTGTTGCTACATCGACCTTTTTTCAGGTGTGCTATACTT; ATGTTTCAAAGAGAATTTGCCCTCCGACTGGTTGCAAAACCTGGAGATAAGTTATACTGC; AGACTCTCAATTAATACACAGCTGTTGGCACGTGTGGACCATCTAATGAAAGTGGGAAAG; AATAACCTCCAGACCACCGNCCAAGGTGGAATCCAGTGTTGTAAAGATAGAACCCTAGAA; TCCACCACCACCCATCAATTTTCACGAATGGGATGGTCTACTAAGGATAACCTTTGTTAG; GAAAAACACGAACTCTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014473 unedited; TATGTACCGCGGCACGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTAAAAAACAATTGTTTTACTGTACATGTGAATAACAAAAAATGGTACGGGGAATGT; GAATAACACGAAATGGTATGGGGAATGTGTGACTAGTGAAGCATATTTTAACTTTTTTAT; TACTGGAGGAACAAAGCTAAAGGCCTAAAAAAGGCCTTACAGTATATAACAGTAAATAGA; AGAGTAACATCTTTATACAATAAACTGTTAAAAATGATAAGTGTAAAGTTGGGCGTCTCT; GCGGCTCCTGAACTTGAAGATTATTATGAATAGATTGGACCAGCATTATATATTAAAAAC; TTTGATACTTAAAACTTTCCAACTTTAAAATTCAAAATCATAAATGGGGACAACAGTAGT; AGTATTGAACCAAAAATAGTCAAGTAAATAATGTCTCAGTAAAGCAAAAGCATTATCTTC; TCAAATACAAAAAATACAAAATTCATTTCTTTTCTTGACCTTGAAAATTTCTGTTTTCCA; AATACCTAGGAAAAATGAATACCTTCTGCGTTGAATCCATGTAGCAATCTGATGAAGTCA; TCTATGTCCATGGAACGGGCCCGTTTGTCACTAAAACCTGTGCTGGTTAGGATTTGCTGT; ATTTTATCTGCTATGCTGAAATCTTCTGGTATTATAATATTATGGACTGAACAGTGAATT; CTGTAGTTTTTTTCCAAGAGTTGTTGCACTGCACTTGATTTAAATGCAGCAGAGAGTGTC; TTGTTTTTTCTAACAAGGGTTTCCCTACTAGACCATCCCATCCTGAAAATTGAGGGTGGN; GGGGGGATCCTAAGTTCTATCCCTACACACTGGATTCCAACTTGGGCGGGGTNCTGAGTT; ATTCTTTCCACTTTCATAAATGGCCACACTTGCAACAGCTGGGATTATTGAAGTCGCATA; TACTATTNCAGGTTGGCACAGCCGAGGGCATCTCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014473
• Insert Size: 1670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014473.2, NP_055288.1
• RefSeq Size: 1557 bp
• RefSeq ORF: 942 bp
• Locus ID: 27292
• UniProt ID: Q9UNQ2
• Cytogenetics: 5q12.1
• Domains: rADc
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene is a methyltransferase that is responsible for dimethylation of adjacent adenosines near the 18S rRNA decoding site. The encoded protein is essential for ribosome biogenesis, although its catalytic activity is not involved in the process. The yeast ortholog of this protein functions in the cytoplasm while this protein functions in the nucleus. [provided by RefSeq, Jan 2017]; Transcript Variant: This variant (1) encodes the longest isoform (1).