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FIP200 (RB1CC1) (NM_014781) Human Untagged Clone

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SKU
SC114884
RB1CC1 (untagged)-Human RB1-inducible coiled-coil 1 (RB1CC1), transcript variant 1
  $1,364.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol FIP200
Synonyms ATG17; CC1; FIP200; PPP1R131
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC114884 sequence for NM_014781 edited (data generated by NextGen Sequencing)
ATGAAGTTATATGTATTTCTGGTTAACACTGGAACTACTCTAACATTTGACACTGAACTT
ACAGTGCAAACTGTGGCAGACCTTAAGCATGCCATTCAAAGCAAATACAAGATTGCTATT
CAACACCAGGTGCTGGTGGTCAATGGAGGAGAATGCATGGCTGCAGATCGAAGAGTGTGT
ACCTACAGTGCTGGGACGGATACAAATCCAATTTTTCTTTTTAACAAAGAAATGATCTTA
TGTGATCGTCCACCTGCTATTCCTAAAACTACCTTTTCGACAGAAAATGACATGGAAATA
AAAGTTGAAGAATCTCTTATGATGCCTGCAGTTTTTCATACTGTTGCTTCAAGGACACAG
CTTGCATTGGAAATGTATGAAGTTGCCAAGAAACTTTGTTCTTTTTGTGAAGGTCTTGTA
CATGATGAACATCTTCAACACCAAGGCTGGGCTGCAATCATGGCCAACCTGGAGGACTGT
TCAAATTCATACCAAAAGCTACTTTTCAAGTTTGAAAGTATTTATTCAAATTATCTGCAG
TCCATAGAAGACATCAAGTTAAAACTTACTCATTTAGGAACTGCAGTTTCAGTAATGGCC
AAGATTCCACTGTTGGAGTGCCTAACCAGACATAGTTACAGAGAATGTTTGGGAAGACTG
GATTCTTTACCTGAACATGAAGACTCAGAAAAAGCTGAGACGAAAAGATCCACTGAACTG
GTGCTCTCTCCTGATATGCCTAGAACAACTAACGAATCTTTGTTAACCTCATTTCCCAAG
TCAGTGGAACATGTGTCCCCAGATACCGCAGATGCTGAAAGTGGCAAAGAAATTAGGGAA
TCTTGTCAAAGTACTGTTCATCAGCAAGATGAAACTACGATTGACACTAAAGATGGTGAT
CTGCCCTTTTTTAATGTCTCTTTGTTAGACTGGATAAATGTTCAAGATAGACCTAATGAT
GTGGAATCTTTGGTCAGGAAGTGCTTTGATTCTATGAGCAGGCTTGATCCAAGGATTATT
CGACCATTTATAGCAGAATGCCGTCAAACTATTGCCAAACTTGATAATCAGAATATGAAA
GCCATTAAAGGACTTGAAGATCGGCTCTACGCCCTGGACCAGATGATTGCTAGCTGTGGC
CGACTGGTGAATGAACAGAAAGAGCTTGCTCAGGGATTTTTAGCTAATCAGAAGAGAGCT
GAAAACTTAAAGGATGCATCTGTATTACCTGATTTATGCCTGAGTCACGCAAATCAGTTG
ATGATTATGTTGCAAAATCATAGAAAACTGTTAGATATTAAGCAGAAGTGTACCACTGCC
AAACAAGAACTAGCAAATAACCTACATGTCAGACTGAAGTGGTGTTGCTTTGTAATGCTT
CATGCTGATCAAGATGGAGAGAAGTTACAAGCTTTGCTCCGCCTCGTAATAGAGCTGTTA
GAAAGAGTCAAAATTGTTGAAGCTCTTAGTACAGTTCCTCAGATGTACTGCTTAGCTGTT
GTTGAGGTTGTAAGAAGAAAAATGTTCATAAAACACTACAGGGAGTGGGCTGGTGCTTTA
GTCAAAGATGGAAAGAGATTATATGAAGCAGAAAAATCAAAAAGGGAATCCTTTGGGAAA
TTATTTAGGAAGTCTTTTTTAAGAAATCGTCTGTTTAGGGGACTGGACTCCTGGCCCCCT
TCCTTTTGTACTCAAAAGCCTCGAAAGTTTGACTGTGAACTTCCAGATATTTCATTAAAA
GATTTACAGTTTCTGCAATCATTTTGTCCTTCGGAAGTTCAGCCATTCCTCAGGGTTCCC
TTACTTTGTGACTTTGAACCTCTACACCAGCATGTACTTGCTCTACATAATTTGGTAAAA
GCAGCACAAAGTTTGGATGAAATGTCACAGACCATTACAGATCTACTGAGTGAACAAAAG
GCATCTGTGAGTCAGACATCCCCACAGTCTGCTTCTTCACCAAGGATGGAAAGTACAGCA
GGAATTACAACTACTACCTCACCGAGAACTCCTCCACCACTGACTGTTCAGGATCCCTTA
TGTCCTGCAGTTTGTCCCTTAGAAGAATTATCTCCAGATAGTATTGATGCACATACGTTT
GATTTTGAAACTATTCCCCATCCAAACATAGAACAGACTATTCACCAAGTTTCTTTAGAC
TTGGATTCATTAGCAGAAAGTCCTGAATCAGATTTTATGTCTGCTGTGAATGAGTTTGTA
ATAGAAGAAAATTTGTCGTCTCCTAATCCTATAAGTGATCCACAAAGCCCAGAAATGATG
GTGGAATCACTTTATTCATCAGTTATCAATGCGATAGACAGTAGACGAATGCAGGATACA
AATGTATGTGGTAAGGAGGATTTTGGAGATCATACTTCTCTGAATGTCCAGTTGGAAAGA
TGTAGAGTTGTTGCCCAAGACTCTCACTTCAGTATACAAACCATTAAGGAAGACCTTTGC
CACTTTAGAACATTTGTACAAAAAGAACAGTGTGACTTCTCAAATTCATTAAAATGTACA
GCAGTAGAAATAAGAAACATTATTGAAAAAGTAAAATGTTCTCTGGAAATAACACTAAAA
GAAAAACATCAAAAAGAACTACTGTCTTTAAAAAATGAATATGAAGGTAAACTTGACGGA
CTAATAAAGGAAACTGAAGAGAATGAAAACAAAATTAAAAAATTGAAGGGAGAGTTAGTA
TGCCTTGAGGAGGTTTTACAAAATAAAGATAATGAATTTGCTTTGGTTAAACATGAAAAA
GAAGCTGTAATCTGCCTGCAGAATGAAAAGGATCAGAAGTTGTTAGAGATGGAAAATATA
ATGCACTCTCAAAATTGTGAAATTAAAGAACTGAAGCAGTCACGAGAAATAGTGTTAGAA
GACTTAAAAAAGCTCCATGTTGAAAATGATGAGAAGTTACAGTTATTGAGGGCAGAACTT
CAGTCCTTGGAGCAAAGTCATCTAAAGGAATTAGAGGACACACTTCAGGTTAGGCACATA
CAAGAGTTTGAGAAGGTTATGACAGACCACAGAGTTTCTTTGGAGGAATTAAAAAAGGAA
AACCAACAAATAATTAATCAAATACAAGAATCTCATGCTGAAATTATCCAGGAAAAAGAA
AAACAGTTACAGGAATTAAAACTCAAGGTTTCTGATTTGTCAGACACGAGATGCAAGTTA
GAGGTTGAACTTGCGTTGAAGGAAGCAGAAACTGATGAAATAAAAATTTTGCTGGAAGAA
AGCAGAGCCCAGCAGAAGGAGACCTTGAAATCTCTTCTTGAACAAGAGACAGAAAATTTG
AGAACAGAAATTAGTAAACTCAACCAAAAGATTCAGGATAATAATGAAAATTATCAGGTG
GGCTTAGCAGAGCTAAGAACTTTAATGACAATTGAAAAAGATCAGTGTATTTCCGAGTTA
ATTAGTAGACATGAAGAAGAATCTAATATACTTAAAGCTGAATTAAACAAAGTAACATCT
TTGCATAACCAAGCATTTGAAATAGAAAAAAACCTAAAAGAACAAATAATTGAACTGCAG
AGTAAATTGGATTCAGAATTGAGTGCTCTTGAAAGACAAAAAGATGAAAAAATTACCCAA
CAAGAAGAGAAATACGAAGCTATTATCCAGAACCTTGAGAAAGACAGACAAAAATTGGTC
AGCAGCCAGGAGCAAGACAGAGAACAGTTAATTCAGAAGCTTAATTGTGAAAAAGATGAA
GCTATTCAGACTGCCCTAAAAGAATTTAAATTGGAGAGAGAAGTTGTTGAGAAAGAGTTA
TTAGAAAAAGTTAAACATCTTGAGAATCAAATAGCAAAAAGTCCTGCCATTGACTCTACC
AGAGGAGATTCTTCAAGCTTAGTTGCTGAACTTCAAGAAAAGCTTCAGGAAGAAAAAGCT
AAGTTTCTAGAACAACTTGAAGAGCAAGAAAAAAGAAAGAATGAAGAAATGCAAAATGTT
CGAACATCTTTGATTGCGGAACAACAGACCAATTTTAACACTGTTTTAACAAGAGAGAAA
ATGAGAAAAGAAAACATAATAAATGATCTTAGTGATAAGTTGAAAAGTACAATGCAGCAA
CAAGAACGGGATAAAGATTTGATAGAGTCACTTTCTGAAGATCGAGCTCGTTTGCTTGAG
GAAAAGAAAAAGCTTGAAGAAGAAGTCAGTAAGTTGCGTAGTAGCAGTTTTGTTCCTTCA
CCATATGTAGCTACAGCCCCAGAACTTTATGGAGCTTGTGCACCTGAACTCCCAGGTGAA
TCAGATAGATCCGCTGTGGAAACAGCAGATGAAGGAAGAGTGGATTCAGCAATGGAGACA
AGCATGATGTCTGTACAAGAAAATATTCATATGTTGTCTGAAGAAAAACAGCGGATAATG
CTGTTAGAACGAACATTGCAATTGAAAGAAGAAGAAAATAAACGGTTAAATCAAAGACTG
ATGTCTCAGAGCATGTCTTCAGTATCTTCAAGGCATTCTGAAAAGATAGCTATTAGAGAT
TTTCAGGTGGGAGATTTGGTACTCATCATCCTAGACGAACGCCATGACAATTATGTGTTA
TTTACTGTTAGTCCTACTTTATATTTTCTACATTCAGAGTCTCTACCTGCCCTGGATCTC
AAACCAGGTGAGGGTGCTTCAGGTGCATCTAGAAGACCCTGGGTACTTGGAAAAGTAATG
GAAAAAGAATACTGTCAAGCCAAAAAGGCACAAAACAGATTTAAAGTTCCTTTGGGGACA
AAGTTTTACAGAGTGAAAGCCGTATCATGGAATAAGAAAGTATAA

Clone variation with respect to NM_014781.4
701 t=>c
5' Read Nucleotide Sequence
>OriGene 5' read for NM_014781 unedited
GCATTTTGTATACGACTCACTATAGGGCGGCCGCGAATCGGCACCANAAACCAAGCCGCG
GCGGTGTCCGCGGCCCTGCCGAGCCCTCGGCGTTGCCTCAGAATCCCCCAGTCGCCTGGG
CCCCTCGGCTCTGACAGGCCGCGGCCTTCTGTCCCCCGGCCCCAGACCCAGAGCCGAGGG
GCCTGCTCGCGTCCTTGTCCGCCCGGACCCCTCCCTGCCTCCTAGAGTTCGGGGCCGCGG
CGGGCGGGCGCCCGGGACGCCGGCGGTTGTGTCGGCTTAGCGGTGCCGAATGGGCGGTTG
GTAACCGCTGCCGAGGACTAGGCGGCGGCGGAAGATGGTGCCGGGGGTCGCTGGCTCTGC
TGCTGCCGCCGGCGAAGGAGGAGGCGTTGCCGGTTTTCTGAGTTTAACCAGTAATGCCAT
TCAGTTGCCAATCTCAAGCAAAGCAAACATAAGCCAGTTTTAATCTACTTTTTAAGAAAA
GTGGTAGTCCTTTTCACAGTGCCTGACGTAACTGTATCAGAGGGTGAGGTATAAGCTCAC
AGAATTCAGATAAATCATCATGAAGTTATATGTATTTCTGGTTAACACTGGAACTACTCT
AACATTTGACACTGAACTTACAGTGCAAACTGTGGCAGACCTTAAGCATGCCATTCAAAG
CAAATACAAGATTGCTATTCAACACCAGGTGCTGGTGGTCAATGGAGGAGAATGCATGGC
TGCAGATCGAAGAGTGTGTACCTACAGTGCTGGGACGGATACAAATCCAATTTTTTTTTT
AACAAGAAAGATCTTATGTGATCGTCACCTGCTATCCTAAACTACCTTTTCGACAGAAAT
GACATGGAAATAAAGTCGAAGAACTCTTATGAGCCGCAGTTTTTATACTGTTGCTTCAGG
ACAAGCTTGCTTTGAAAGGTTGAACTGCCAGTAACTTGTTCCTCNGTGGAGGTCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_014781 unedited
ACTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTACAGGTTC
AAATCTTTATTGTTTTAAAATTGTACAGTTCTTTTACAATACAATATTTTGTTATTTAAA
TGACCTCTAAATGGTTAATGTGCAATGAATATCTTTTGTTCTGAAACTCAACCATGAATT
ACGATTTCATCAATATGATTATTCTAGAGTCATAAAGATGTTGGGAAGGTTTGAGTCTGC
ATTTGAAATGGTTAGTAGCACAGACTCTTCCCTTTAGAACCCAGATGACCAATCCACTGA
AAGTGCTTTTGAAGATTGCTGATTACTTATGGCTGATCTTAAAATCAGTCAATCAAATTA
CAGTTCCTTTTTTTTTTTGAAACATCAAAGGGCATTTAAAAAAACTGAAACAATTTGCTT
TAGTATCTACTGAGGCAAACTCAAAATGATTATCAGCATGCAATAACACTCCTCTTTATT
TTAAATAGTTCTGAATAATTTATACATTTTATAAAGAATGTGTTAAAGAGTGCAAGTATT
CTGATACTGATTTCACAAAAGGACAAATGCTGGTAATAATAACATTTAACATCTAAGTTC
AAGCTAGTGTATATTTAACAGGCAATTAATATGGCTCATCATAAGCCACAATGCACAAGG
TATGCCCTTTGAGAAAATGAAGTAGTTTGGTCCGCATTTCTAAAGTTTGCCTGGGTAAAA
AAAAAAACCAAAAACCAAAACCCTTTTAATTCACCCAAGATTCTGATGAATTAATTATTC
CTAAAATGAGCCCGTTAAAAAGTAAACGATGTCCACCAGTGAAGTTTTTGGACCCCTGAC
TTTGCATAAAAAATGGCTGCTGTTTTTGGAGGATGAATTGACCCGCGGCCAAATCAAAAA
AATGCCTAGAAGGATTAATTTGGCCCAAGTAACCTTCTTATCATGGAAGGCTTTCCTTTG
AAATTTGCCCCCAGAAACTTAACCA
Restriction Sites NotI-NotI |
ACCN NM_014781
Insert Size 6000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014781.3, NP_055596.2
RefSeq Size 6636 bp
RefSeq ORF 4785 bp
Locus ID 9821
UniProt ID Q8TDY2
Cytogenetics 8q11.23
Protein Families Druggable Genome
Summary The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Nov 2009
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript matching the genomic sequence was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
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Documents
Resources
"NM_014781" in other vectors (6)
SKU Description Size Price
RC202221 RB1CC1 (Myc-DDK-tagged)-Human RB1-inducible coiled-coil 1 (RB1CC1), transcript variant 1 10 ug
$589.00 $1,363.00
RC202221L1 Lenti-ORF clone of RB1CC1 (Myc-DDK-tagged)-Human RB1-inducible coiled-coil 1 (RB1CC1), transcript variant 1 10 ug
$1,663.00
RC202221L2 Lenti-ORF clone of RB1CC1 (mGFP-tagged)-Human RB1-inducible coiled-coil 1 (RB1CC1), transcript variant 1 10 ug
$1,663.00
RC202221L3 Lenti-ORF clone of RB1CC1 (Myc-DDK-tagged)-Human RB1-inducible coiled-coil 1 (RB1CC1), transcript variant 1 10 ug
$1,663.00
RC202221L4 Lenti-ORF clone of RB1CC1 (mGFP-tagged)-Human RB1-inducible coiled-coil 1 (RB1CC1), transcript variant 1 10 ug
$1,663.00
RG202221 RB1CC1 (tGFP-tagged) - Human RB1-inducible coiled-coil 1 (RB1CC1), transcript variant 1 10 ug
$789.00 $1,563.00

file-alt Citations

Rabaptin5 targets autophagy to damaged endosomes and Salmonella vacuoles via FIP200 and ATG16L1
Millarte, Schlienger, Kälin et al
EMBO Rep (2021)
Cited by 14
AI Summary: In this study, OriGene's human FIP200 cDNA (catalog #SC114884) was used to investigate the role of FIP200 as a Rabaptin5 interactor in the selective autophagy of damaged endosomes and Salmonella vacuoles.
Snippet: …Rabaptin5 was previously known as a major regulator of early endosome and . Save;The cDNA of human FIP200 was purchased from OriGene (SC114884), pmCherry_Gal3 was a gift from Hemmo Meyer (Addgene plasmid #85662) (Papadopoulos et al, 2017),
Showing 1-1 of 1 papers.
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