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NELF (NSMF) (NM_015537) Human Untagged Clone

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SKU
SC114669
NSMF (untagged)-Human nasal embryonic LHRH factor (NELF), transcript variant 2
  $492.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NELF
Synonyms HH9; NELF
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_015537, the custom clone sequence may differ by one or more nucleotides


ATGGGCGCCGCCGCCTCCAGGAGGAGGGCGCTGAGGAGCGAGGCCATGTCCTCGGTGGCGGCCAAAGTGC
GAGCAGCCCGAGCGTTTGGAGAGTACCTGTCCCAGAGTCACCCTGAGAACCGCAACGGCGCAGATCACCT
GCTGGCTGATGCCTACTCTGGCCACGACGGGTCCCCCGAGATGCAGCCGGCCCCCCAGAACAAGCGCCGC
CTGTCCCTCGTCTCCAACGGCTGCTACGAGGGCAGCCTCTCAGAGGAGCCCAGCATTAGGAAGCCCGCAG
GCGAGGGCCCTCAGCCTCGAGTGTACACCATCTCTGGGGAGCCTGCCCTGCTGCCCAGCCCTGAGGCGGA
GGCCATTGAGCTGGCGGTGGTGAAGGGGCGGCGGCAGCGGCACCCTCACCATCACAGCCAGCCCCTGCGC
GCCAGCCCTGGTGGCAGCCGGGAGGACGTCAGCAGGCCCTGCCAGAGCTGGGCGGGCAGCCGCCAGGGCT
CCAAGGAGTGCCCCGGATGTGCCCAGCTGGCTCCTGGCCCCACCCCTCGGGCCTTTGGGCTGGACCAGCC
ACCTCTGCCTGAGACCTCCGGTCGCCGCAAGAAGCTGGAGAGGATGTACAGCGTTGACCGTGTGTCTGAC
GACATCCCTATTCGTACCTGGTTCCCCAAGGAAAATCTTTTCAGCTTCCAGACAGCAACCACAACTATGC
AAGCGGTGTTCAGGGGCTACGCGGAGAGGAAGCGCCGGAAACGGGAGAATGATTCCGCGTCTGTAATCCA
GAGGAACTTCCGCAAACACCTGCGCATGGTCGGCAGCCGGAGGGTGAAGGCCCAGACGTTCGCTGAGCGG
CGCGAGCGGAGCTTCAGCCGGTCCTGGAGCGACCCCACCCCCATGAAAGCCGACACTTCCCACGACTCCC
GAGACAGCAGTGACCTGCAGAGCTCCCACTGCACGCTGGACGAGGCCTTCGAGGACCTGGACTGGGACAC
TGAGAAGGGCCTGGAGGCTGTGGCCTGCGACACCGAAGGCTTCGTGCCACCAAAGGTCATGCTCATTTCC
TCCAAGGTGCCCAAGGCTGAGTACATCCCCACTATCATCCGCCGGGATGACCCCTCCATCATCCCCATCC
TCTACGACCATGAGCACGCAACCTTCGAGGACATCCTTGAGGAGATAGAGAGGAAGCTGAACGTCTACCA
CAAGGGAGCCAAGATCTGGAAAATGCTGATTTTCTGCCAGGGAGGTCCTGGACACCTCTATCTCCTCAAG
AACAAGGTGGCCACCTTTGCCAAAGTGGAGAAGGAAGAGGACATGATTCACTTCTGGAAGCGGCTGAGCC
GCCTGATGAGCAAAGTGAACCCAGAGCCGAACGTCATCCACATCATGGGCTGCTACATTCTGGGGAACCC
CAATGGAGAGAAGCTGTTCCAGAACCTCAGGACCCTCATGACTCCTTATAGGGTCACCTTCGAGTCACCC
CTGGAGCTCTCAGCCCAAGGGAAGCAGATGATCGAGACGTACTTTGACTTCCGGTTGTATCGCCTGTGGA
AGAGCCGCCAGCACTCGAAGCTGCTGGACTTTGACGACGTCCTGTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_015537 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGCCGCCAGGG
CTCCAAGGAGTGCCCCGGATGTGCCCAGCTGGCTCCTGGCCCCACCCCTCGGGCCTTTGG
GCTGGACCAGCCACCTCTGCCTGAGACCTCCGGTCGCCGCAAGAAGCTGGAGAGGATGTA
CAGCGTTGACCGTGTGTCTGACGACATCCCTATTCGTACCTGGTTCCCCAAGGAAAATCT
TTTCAGCTTCCAGACAGCAACCACAACTATGCAAGCCATCTCGGTGTTCAGGGGCTACGC
GGAGAGGAAGCGCCGGAAACGGGAGAATGATTCCGCGTCTGTAATCCAGAGGAACTTCCG
CAAACACCTGCGCATGGTCGGCAGCCGGAGGGTGAAGGCCCAGACGTTCGCTGAGCGGCG
CGAGCGGAGCTTCAGCCGGTCCTGGAGCGACCCCACCCCCATGAAAGCCGACACTTCCCA
CGACTCCCGAGACAGCAGTGACCTGCAGAGCTCCCACTGCACGCTGGACGAGGCCTTCGA
GGACCTGGACTGGGACACTGAGAAGGGCCTGGAGGCTGTGGCCTGCGACACCGAAGGCTT
CGTGCCACCAAAGGTCATGCTCATTTCCTCCAAGGTGCCCAAGGCTGAGTACATCCCCAC
TATCATCCGCCGGGATGACCCCTCCATCATCCCCATCCTCTACGACCATGAGCACGCAAC
CTTCGAGGACATCCTTGAGGAGATAGAGAGGAAGCTGAACGTCTACCCAAGGGAGCCAAG
ATCTGGAAATGGCTGATTTTCTGCCAGGGAGTCCTGGACACTCTATCTCTCAGAACAGGG
TTGCCCCTTTGCCAAGTGGAGAAGGAGAGACATGATTACTT
Restriction Sites NotI-NotI |
ACCN NM_015537
Insert Size 4230 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015537.3, NP_056352.3
RefSeq Size 2972 bp
RefSeq ORF 1587 bp
Locus ID 26012
UniProt ID Q6X4W1
Cytogenetics 9q34.3
Domains IQ
Protein Families Druggable Genome
Summary The protein encoded by this gene is involved in guidance of olfactory axon projections and migration of luteinizing hormone-releasing hormone neurons. Defects in this gene are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, May 2010
Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.
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Documents
Resources
"NM_015537" in other vectors (5)
SKU Description Size Price
RC210092 NSMF (Myc-DDK-tagged)-Human nasal embryonic LHRH factor (NELF), transcript variant 2 10 ug
$492.00
RC210092L3 Lenti ORF clone of Human nasal embryonic LHRH factor (NELF), transcript variant 2, Myc-DDK-tagged 10 ug
$792.00
RC210092L4 Lenti ORF clone of Human nasal embryonic LHRH factor (NELF), transcript variant 2, mGFP tagged 10 ug
$792.00
RG210092 NSMF (tGFP-tagged) - Human nasal embryonic LHRH factor (NELF), transcript variant 2 10 ug
$489.00 $692.00
SC322751 NSMF (untagged)-Human nasal embryonic LHRH factor (NELF), transcript variant 2 10 ug
$492.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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