NECAP1 (NM_015509) Human Untagged Clone

SKU SC114658

NECAP1 (untagged)-Human NECAP endocytosis associated 1 (NECAP1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NECAP1
• Synonyms: DEE21; EIEE21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114658 sequence for NM_015509 edited (data generated by NextGen Sequencing); ATGGCGACCGAGTTGGAGTACGAGTCTGTGCTGTGTGTGAAGCCAGACGTCAGCGTCTAC; CGGATTCCGCCCCGGGCCTCCAACCGCGGTTACAGGGCCTCTGACTGGAAATTAGACCAG; CCTGATTGGACTGGTCGCCTCCGAATCACTTCAAAAGGGAAGACTGCCTATATCAAACTC; GAGGATAAAGTTTCAGGGGAGCTCTTTGCTCAGGCACCAGTAGAACAATATCCTGGTATT; GCTGTGGAGACGGTGACAGATTCTAGCCGCTACTTTGTAATCCGGATCCAGGATGGTACT; GGGCGCAGTGCTTTCATTGGCATTGGCTTCACAGATCGGGGAGATGCCTTCGACTTTAAT; GTCTCCTTGCAGGATCACTTCAAGTGGGTAAAGCAGGAATCTGAGATTTCCAAGGAATCT; CAAGAAATGGATGCTCGTCCTAAGTTGGATCTGGGCTTCAAGGAAGGACAAACCATCAAG; TTGTGTATCGGGAACATTACAAACAAGAAAGGAGGTGCTTCTAAGCCCAGGACTGCAAGG; GGTGGGGGTCTGAGCTTACTCCCACCCCCGCCAGGAGGCAAAGTCACTATTCCCCCACCA; TCCTCCTCAGTTGCCATCAGCAATCATGTCACCCCACCACCCATTCCGAAATCTAACCAT; GGAGGCAGTGATGCAGATATCCTTTTAGATTTGGATTCTCCTGCTCCTGTCACGACACCA; GCACCAACTCCAGTTTCTGTAAGCAATGACTTGTGGGGAGACTTCAGCACTGCCTCCAGC; TCTGTTCCAAACCAGGCACCACAGCCATCCAACTGGGTCCAGTTCTGA; ; Clone variation with respect to NM_015509.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015509 unedited; ATTTTGGTATACGATTTACTATAGGGCCGCGCCGCGCAATTCGGCACGAGGGATGGCGAC; CGAGTTGGAGTACGAGTCTGTGCTGTGTGTGAAGCCAGACGTCAGCGTCTACCGGATTCC; GCCCCGGGCCTCCAACCGCGGTTACAGGGCCTCTGACTGGAAATTAGACCAGCCTGATTG; GACTGGTCGCCTCCGAATCACTTCAAAAGGGAAGACTGCCTATATCAAACTCGAGGATAA; AGTTTCAGGGGAGCTCTTTGCTCAGGCACCAGTAGAACAATATCCTGGTATTGCTGTGGA; GACGGCGACAGATTCTAGCCGCTACTTTGTAATCCGGATCCAGGATGGTACTGGGCGCAG; TGCTTTCATTGGCATTGGCTTCACAGATCGGGGAGATGCCTTCGACTTTAATGTCTCCTT; GCAGGATCACTTCAAGTGGGTAAAGCAGGAATCTGAGATTTCCAAGGAATCTCAAGAAAT; GGATGCTCGTCCTAAGTTGGATCTGGGCTTCAAGGAAGGACAAACCATCAAGTTGTGTAT; CGGGAACATTACAAACAAGAAAGGAGGTGCTTCTAAGCCCAGGACTGCAAGGGGCGGGGG; CCTGAGCTTACTCCCACCCCCGGCAGCAGGCAAAAGTCACTATTTCCCCTACCATCCTCC; TCAGTTGCCATCAGCAATCATGTCACCCCACCACCCATTTCGAAATCTAACCATGGAGGC; AGTGATGCAGATATTCTTTTACATCTGGATTCTCCTGCTCCTGGCACGACACCAACACCC; ACTCCCGTTCTCTGTGAGCAATGAACTTGTGGGAGAGAATTCAACACTGTCTCTCAGCTC; TTGTTCCAACCAGCCACCACAGCCATTCAACTTGGCCCAGNTCTGAATGCCCTTGGCCGG; GCACTTAACGGCAACACTTGAGGAACATAAAAGGACCTTTGAGGCCACCAATCTGCGAGG; GAAGTAGGGACCCCATTTCTCCCCACACCAGAGTGCCCGACCATTTTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_015509
• Insert Size: 2920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015509.2, NP_056324.2
• RefSeq Size: 2563 bp
• RefSeq ORF: 828 bp
• Locus ID: 25977
• UniProt ID: Q8NC96
• Cytogenetics: 12p13.31
• Summary: This gene encodes a protein containing two characteristic WXXF motifs. The encoded protein localizes to clathrin-coated vesicles, where it binds components of the adapter protein complexes and aids in endocytosis. Loss of function of this gene results in early infantile epileptic encephalopathy-21. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]; Transcript Variant: This variant (1) encodes a functional protein.