ARSB (NM_000046) Human Untagged Clone

SKU SC114442

ARSB (untagged)-Human arylsulfatase B (ARSB), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARSB
• Synonyms: ASB; G4S; MPS6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114442 sequence for NM_000046 edited (data generated by NextGen Sequencing); ATGGGTCCGCGCGGCGCGGCGAGCTTGCCCCGAGGCCCCGGACCTCGGCGGCTGCTCCTC; CCCGTCGTCCTCCCGCTGCTGCTGCTGCTGTTGTTGGCGCCGCCGGGCTCGGGCGCCGGG; GCCAGCCGGCCGCCCCACCTGGTCTTCTTGCTGGCAGACGACCTAGGCTGGAACGACGTC; GGCTTCCACGGCTCCCGCATCCGCACGCCGCACCTGGACGCGCTGGCGGCCGGCGGGGTG; CTCCTGGACAACTACTACACGCAGCCGCTGTGCACGCCGTCGCGGAGCCAGCTGCTCACT; GGCCGCTACCAGATCCGTACAGGTTTACAGCACCAAATAATCTGGCCCTGTCAGCCCAGC; TGTGTTCCTCTGGATGAAAAACTCCTGCCCCAGCTCCTAAAAGAAGCAGGTTATACTACC; CATATGGTCGGAAAATGGCACCTGGGAATGTACCGGAAAGAATGCCTTCCAACCCGCCGA; GGATTTGATACCTACTTTGGATATCTCCTGGGTAGTGAAGATTATTATTCCCATGAACGC; TGTACATTAATTGACGCTCTGAATGTCACACGATGTGCTCTTGATTTTCGAGATGGCGAA; GAAGTTGCAACAGGATATAAAAATATGTATTCAACAAACATATTCACCAAAAGGGCTATA; GCCCTCATAACTAACCATCCACCAGAGAAGCCTCTGTTTCTCTACCTTGCTCTCCAGTCT; GTGCATGAGCCCCTTCAGGTCCCTGAGGAATACTTGAAGCCATATGACTTTATCCAAGAC; AAGAACAGGCATCACTATGCAGGAATGGTGTCCCTTATGGATGAAGCAGTAGGAAATGTC; ACTGCAGCTTTAAAAAGCAGTGGGCTCTGGAACAACACGGTGTTCATCTTTTCTACAGAT; AACGGAGGGCAGACTTTGGCAGGGGGTAATAACTGGCCCCTTCGAGGAAGAAAATGGAGC; CTGTGGGAAGGAGGCGTCCGAGGGGTGGGCTTTGTGGCAAGCCCCTTGCTGAAGCAGAAG; GGCGTGAAGAACCGGGAGCTCATCCACATCTCTGACTGGCTGCCAACACTCATGAAGCTG; GCCAGGGGACACACCAATGGCACAAAGCCTCTGGATGGCTTCGACGTGTGGAAAACCATC; AGTGAAGGAAGCCCATCCCCCAGAATTGAGCTGCTGCATAATATTGACCCGAACTTCGTG; GACTCTTCACCGTGTCCCAGGAACAGCATGGCTCCAGCAAAGGATGACTCTTCTCTTCCA; GAATATTCAGCCTTTAACACATCTGTCCATGCTGCAATTAGACATGGAAATTGGAAACTC; CTCACGGGCTACCCAGGCTGTGGTTACTGGTTCCCTCCACCGTCTCAATACAATGTTTCT; GAGATACCCTCATCAGACCCACCAACCAAGACCCTCTGGCTCTTTGATATTGATCGGGAC; CCTGAAGAAAGACATGACCTGTCCAGAGAATATCCTCACATCGTCACAAAGCTCCTGTCC; CGCCTACAGTTCTACCATAAACACTCAGTCCCCGTGTACTTCCCTGCACAGGACCCCCGC; TGTGATCCCAAGGCCACTGGGGTGTGGGGCCCTTGGATGTAG; ; Clone variation with respect to NM_000046.3; 1072 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000046 unedited; GTCAGCTTTTGTAATACGACTTACNTATAGGGCGGCCGCGATTCGGCACGAGGGACAAGG; ATGGGTCCGCGCGGCGCGGCGAGCTTGCCCCGAGGCCCCGGACCTCGGCGGCTGCTCCTC; CCCGTCGTCCTCCCGCTGCTGCTGCTGCTGTTGTTGGCGCCGCCGGGCTCGGGCGCCGGG; GCCAGCCGGCCGCCCCACCTGGTCTTCTTGCTGGCAGACGACCTAGGCTGGAACGACGTC; GGCTTCCACGGCTCCCGCATCCGCACGCCGCACCTGGACGCGCTGGCGGCCGGCGGGGTG; CTCCTGGACAACTACTACACGCAGCCGCTGTGCACGCCGTCGCGGAGCCAGCTGCTCACT; GGCCGCTACCAGATCCGTACAGGTTTACAGCACCAAATAATCTGGCCCTGTCAGCCCAGC; TGTGTTCCTCTGGATGAAAAACTCCTGCCCCAGCTCCTAAAAGAAGCAGGTTATACTACC; CATATGGTCGGAAAATGGCACCTGGGAATGTACCGGAAAGAATGCCTTCCAACCCGCCGA; GGATTTGATACCTACTTTGGATATCTCCTGNGTAGTGAAGATTATTATTCCCATGAACGC; TGTACATTAATTGACGCTCTGAATGTCACACGATGTGCTCTTGATTTTCGAGATGGCGAA; GAAGTTGCAACAGGATATAAAAATATGTATTCAACANACATATTCACCAAAAGGGCTATA; GCCCTCATAACTAACCATCCACCAGAGAAGCCTCTGTTTCTCTACCTTGCTCTCCAGTCT; GTGCATGAGCCNCCTTCAGTCCCTNGAGAATACTTGAAGCCATATGACTTTATCCAAGAC; AGAACAGGCATCACTATGCAGGAATGGTGTCCCTTTATGATGAAGCAGTAGGAAATGTCA; CTGCAGCTTTTAAAAGCAGTGGGCTCTGGAACAACACGTGTTCATCTTTCTACAGATACN; GAGGGCAGACTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000046 unedited; NGGGTACTCTATGNCCGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTGAGTGTTGCAGATTTTATCAGCTTCTTAAATGCATTAGGGGTTGAAATTA; GACACCTCGGTGTGGTTTAAGAGCAAGAGAAGGGCCAAGTGAACCCAGGTTGGGATAACA; AATGAGACAAGAGTCGTGAGAAAAGGCCTGAGGTCCAACTTCCAATTGAAAGGTTTTCTA; GCCTCCCTGAAATCCTACATCCAAGGGCCCCACACCCCAGTGGCCTTGGGATCACAGCGG; GGGTCCTGTGCAGGGAAGTACACGGGGACTGAGTGTTTATGGTAGAACTGTAGGCGGGAC; AGGAGCTTTGTGACGATGTGAGGATATTCTCTGGACAGGTCATGTCTTTCTTCAGGGTCC; CGATCAATATCAAAGAGCCAGAGGGTCTTGGTTGGTGGGTCTGATGAGGGTATCTCAGAA; ACATTGTATTGAGACGGTGGAGGGAACCAGTAACCACAGCCTGGGTAGCCCGTGAGGAGT; TTCCAATTTCCATGTCTAATTGCAGCATGGACAGATGTGTTAAAGGCTGAATATTCTGGA; AGAGAAGAGTCATCCTTTGCTGGAGCCATGCTGTTCCTGGGACACGGTGAAGAGTCCACG; AAGTTCGGGTCAATATTATGCAGCAGCTCAATTCTGGGGGATGGGCTTCCTTCACTGATG; GTTTTCCACACGTCGAAGCCATCCAGAGGCTTTGTGCCATTGGTGTGTCCCCTGCCCAGC; TTCATGAGTGTTGGCAGCCAGTCAGAGATGTGGATGAAGCTCCGGTTCTTTACGCCCTTT; TGCTTTAGCAAGGGGCTTGCCACAAAGCCCACCCTTGGGAAGCCTTCCTTCCACAGCTTC; A;
• Restriction Sites: NotI-NotI
• ACCN: NM_000046
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000046.2, NP_000037.2
• RefSeq Size: 6089 bp
• RefSeq ORF: 1602 bp
• Locus ID: 411
• UniProt ID: P15848
• Cytogenetics: 5q14.1
• Domains: Sulfatase
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Summary: Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016]; Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments.