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RAPGEFL1 (NM_016339) Human Untagged Clone

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SKU
SC114326
RAPGEFL1 (untagged)-Human Rap guanine nucleotide exchange factor (GEF)-like 1 (RAPGEFL1)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RAPGEFL1
Synonyms Link-GEFII
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC114326 sequence for NM_016339 edited (data generated by NextGen Sequencing)
ATGGAGGGCCCTGAAGGGCTGGGCCGGAAGCAAGCCTGTCTAGCCATGCTTCTCCATTTC
TTGGACACCTACCAGGGGCTGCTTCAAGAGGAAGAGGGGGCCGGCCACATCATCAAGGAT
CTATACCTGCTAATTATGAAGGACGAGTCCCTTTACCAGGGCCTCCGAGAGGACACTCTG
AGGCTGCACCAGCTGGTGGAGACGGTGGAACTAAAGATTCCAGAGGAGAACCAGCCACCC
AGCAAGCAGGTGAAGCCACTCTTCCGCCACTTCCGCCGGATAGACTCCTGTCTGCAGACC
CGGGTGGCCTTCCGGGGCTCTGATGAGATCTTCTGCCGTGTATACATGCCTGACCACTCT
TATGTGACCATACGCAGCCGCCTTTCAGCATCTGTGCAGGACATTCTGGGCTCTGTGACG
GAGAAACTTCAATATTCAGAGGAGCCCGCGGGGCGTGAGGATTCCCTCATCCTGGTAGCT
GTGTCCTCCTCTGGAGAGAAGGTCCTTCTCCAGCCCACTGAGGACTGTGTTTTCACCGCA
CTGGGCATCAACAGCCACCTGTTTGCCTGTACTCGGGACAGCTATGAGGCTCTGGTGCCC
CTCCCCGAGGAGATCCAGGTCTCCCCTGGAGACACAGAGATCCACCGAGTGGAGCCTGAG
GACGTTGCCAACCACCTAACTGCCTTCCACTGGGAGCTGTTCCGATGTGTGCATGAGCTG
GAGTTCGTGGACTACGTGTTCCACGGGGAGCGCGGCCGCCGGGAGACGGCCAACTTGGAG
CTGCTGCTGCAGCGCTGCAGCGAGGTCACGCACTGGGTGGCCACCGAAGTGCTGCTCTGC
GAGGCCCCGGGCAAGCGCGCGCAGCTGCTCAAGAAGTTCATCAAGATCGCGGCCCTCTGC
AAGCAGAACCAGGACCTGCTGTCTTTCTACGCCGTGGTCATGGGGCTGGACAACGCCGCT
GTCAGCCGCCTTCGACTCACCTGGGAGAAGCTGCCAGGGAAATTCAAGAACTTGTTTCGC
AAATTTGAGAACCTGACGGACCCCTGCAGGAACCACAAAAGCTACCGAGAAGTGATCTCC
AAAATGAAGCCCCCTGTGATTCCCTTCGTGCCTCTGATCCTCAAAGACCTGACTTTCCTG
CACGAAGGGAGTAAGACCCTTGTAGATGGTTTGGTGAACATCGAGAAGCTGCATTCAGTG
GCCGAAAAAGTGAGGACAATCCGCAAATACCGGAGCCGGCCCCTTTGCCTGGACATGGAG
GCATCCCCCAATCACCTGCAGACCAAGGCCTATGTGCGCCAGTTTCAGGTCATCGACAAC
CAGAACCTCCTCTTCGAGCTCTCCTACAAGCTGGAGGCAAACAGTCAGTGA

Clone variation with respect to NM_016339.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_016339 unedited
TCCCCGCCCCGTTGCCGCTTAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG
AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
TTCGGCACGAGGGAACCGGGTTCTGGAGCGGCTTGCTGGAGGGGCTACCAGGGACAGCGC
CGCCTCAGATATCCTGCTGGATGACATTGTCCTTACCCATTCTCTCTTCCTCCCGACGGA
GAAATTTCTGCAGGAGCTACACCAGTACTTTGTTCGGGCAGGAGGCATGGAGGGCCCTGA
AGGGCTGGGCCGGAAGCAAGCCTGTCTAGCCATGCTTCTCCATTTCTTGGACACCTACCA
GGGGCTGCTTCAAGAGGAAGAGGGGGCCGGCCACATCATCAAGGATCTATACCTGCTAAT
TATGAAGGACGAGTCCCTTTACCAGGGCCTCCGAGAGGACACTCTGAGGCTGCACCAGCT
GGTGGAGACGGTGGAACTAAAGATTCCAGAGGAGAACCAGCCACCCAGCAAGCAGGTGAA
GCCACTCTTCCGCCACTTCCGCCGGATAGACTCCTGTCTGCAGACCCGGGTGGCCTTCCG
GGGCTCTGATGAGATCTTCTGCCGTGTATACATGCCTGACCACTCTTATGTGACCATACG
CAGCCGCCTTTCAGCATCTGTGCAGGACATTCTGGGCTCTGTGACGGAGAAACTTCAATA
TTCAGAGGAGCCCGCGGGGCGTGAGGATTCCCTCATCCTGGTAGCTGTGTCCTCCTCTGG
AGAGAAGGTCCTTCTCCAGCCCCACTGAGACTGTGTTTTCACCGCACTGGGCATCAACAG
CCACCTGTTTGCCTGTACTCGGGACAGCTATGAAGCTCTGGTGCCCCCTCCCCGAGAGAC
CAGGTCTCCCTGNAGAACAGAGATCCACGAGTGGAGCCTGAGACGTTGCCNNACCACTAC
TGCCTTNCACTGGAGCTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_016339 unedited
TATGTACCGCGCCGCAATCNAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTGAATCATAAACTTTATCGGCTTTTTCTCCACCCAGCGGTTTTT
GCAATGACAACCCCGCCTGTACAATACACATGGTTCACTCTGTTAAAACTGCAGGGCAAC
CGGAGGGGGCTGGGGGTCCCTGGGCAGGCATTAAGGCTAGGGGTAAACAACAACCAAAAC
TGGGCCAGTGGGTCCCAATGTCCCACCCAAAAGGTGGTGGCAGCTGGAGTTCCAAAGCTG
TTGGAAGGGGTCAAGAAGAAAGCTGGGAAACCAGGGGCTTCAGGGAGAGGACAGTGCATC
AACAGTAGCAGGGAGGGGGCAAGGAGCAAAAGGGGGCCCATGGGACACTGTCAGGGCAAG
AGGGCATGGGACACAACACCTCCTATGTCCATAGAGGATTTTTGCTGCAGGAACAAGACA
GATCACTGTATAAAAGGGTCTGTACAACATTACCTATGATACAATGTTCACATATGATAC
AAGGCTCTTTCCCTCTTTGAGTTTTAAAAAATAAATGTACAATTCCAGAGCTTTGGGTAA
AAAAATATACACCCTCATAGCAAGAGCAACAGCTGCTTTTGCTACGGCTGTTGCTGTCTC
TTCAAAAGGGAAAGAGGTGGTGCCAGAAAGAAAGGTGAGTCTGTGCCANGAAACCTTACC
CAGTAGCTGGCAGGAGCCCCTGACCCCTCAAGCCTTTAAGTTGTTTAAGGGAAAGGGAGG
GATTAAAAGGTATAAAGGCCTTGGCCGAATCCAAGGCGCCTTTAAGGCTCTGGCATTCCC
CTCCCCCCTTTTGGGCTTGCCTGCCCAAGTATAGGGATCCCCCCCACCCAACTTTTAGGG
GAGGACAAAGGGGAATCAAAAAAGAGGGCCAAAGCCCTTGTGTTGTGTTCAAACC
Restriction Sites NotI-NotI |
ACCN NM_016339
Insert Size 3750 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016339.1, NP_057423.1
RefSeq Size 3340 bp
RefSeq ORF 1371 bp
Locus ID 51195
UniProt ID Q9UHV5
Cytogenetics 17q21.1
Domains RasGEF
Protein Families Druggable Genome
Summary Probable guanine nucleotide exchange factor (GEF).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) encodes the longest isoform (3). Sequence Note: This RefSeq was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
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"NM_016339" in other vectors (4)
SKU Description Size Price
RC215151 RAPGEFL1 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF)-like 1 (RAPGEFL1) 10 ug
$457.00
RC215151L3 Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF)-like 1 (RAPGEFL1), Myc-DDK-tagged 10 ug
$757.00
RC215151L4 Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF)-like 1 (RAPGEFL1), mGFP tagged 10 ug
$757.00
RG215151 RAPGEFL1 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF)-like 1 (RAPGEFL1) 10 ug
$489.00 $657.00

file-alt Citations

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