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ECT2 (NM_018098) Human Untagged Clone

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SKU
SC113779
ECT2 (untagged)-Human epithelial cell transforming sequence 2 oncogene (ECT2)
  $450.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ECT2
Synonyms ARHGEF31
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_018098 edited
GAATCGGCACGAGGGCCTAGAGATAGCAAGAAAACGGCACAAGGTTATTGGCACTTTTAG
GAGTCCTCATGGCCAAACCCGACCCCCAGCTTCTCTTAAGCATATTCACCTAATGCCTCT
TTCTCAGATTAAGAAGGTATTGGACATAAGAGAGACAGAAGATTGCCATAATGCTTTTGC
CTTGCTTGTGAGGCCACCAACAGAGCAGGCAAATGTGCTACTCAGTTTCCAGATGACATC
AGATGAACTTCCAAAAGAAAACTGGCTAAAGATGCTGTGTCGACATGTAGCTAACACCAT
TTGTAAAGCAGATGCTGAGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAA
TACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTCAAA
AAAGGTTACAAGAGCATTCTCTTTCTCCAAAACTCCAAAAAGAGCTCTTCGAAGGGCTCT
TATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGATAAGCATGTAAT
GAGTCGTCTTTCTAGCACATCATCATTAGCAGGTATCCCTTCTCCCTCCCTTGTCAGCCT
TCCTTCCTTCTTTGAAAGGAGAAGTCATACGTTAAGTAGATCTACAACTCATTTGATATG
AAGCGTTACCAAAATCTTAAATTATAGAAATGTATAGACACCTCATACTCAAATAAGAAA
CTGACTTAAATGGTACTTGTAATTAGCACTTGGTGAAAGCTGGAAGGAAGATAAATAACA
CTAAACTATGCTATTTGATTTTTCTTCTTGAAAGAGTAAGGTTTACCTGTTACATTTTCA
AGTTAATTCATGTAAAAAATGATAGTGATTTTGATGTAATTTATCTCTTGTTTGAATCTG
TCATTCAAAGGCCAATAATTTAAGTTGCTATCAGCTGATATTAGTAGCTTTGCAACCCTG
ATAGAGTAAATAAATTTTATGGGCGGGTGCCAAATACTGCTGTGAATCTATTTGTATAGT
ATCCATGAATGAATTTATGGAAATAGATATTTGTGCAGCTCAATTTATGCAGAGATTAAA
TGACATCATAATACTGGATGAAAACTTGCATAGAATTCTGATTAAATAGTGGGTCTGTTT
CACATGTGCAGTTTGAAGTATTTAAATAACCACTCCTTTCACAGTTTATTTTCTTCTCAA
GCGTTTTCAAGATCTAGCATGTGGATTTTAAAAGATTTGCCCTCATTAACAAGAATAACA
TTTAAAGGAGATTGTTTCAAAATATTTTTGCAAATTGAGATAAGGACAGAAAGATTGAGA
AACATTGTATATTTTGCAAAAACAAGATGTTTGTAGCTGTTTCAGAGAGAGTACGGTATA
TTTATGGTAATTTTATCCACTAGCAAATCTTGATTTAGTTTGATAGTGTGTGGAATTTTA
TTTTGAAGGATAAGACCATGGGAAAATTGTGGTAAAGACTGTTTGTACCCTTCATGAAAT
AATTCTGAAGTTGCCATCAGTTTTACTAATCTTCTGTGAAATGCATAGATATGCGCATGT
TCAACTTTTTATTGTGGTCTTATAATTAAATGTAAAATTGAAAATTCATTTGCTGTTTCA
AAGTGTGATATCTTTCACAATAGCCTTTTTATAGTCAGTAATTCAGAATAATCAAGTTCA
TATGGATAAATGCATTTTTATTTCCTATTTCTTTAGGGAGTGCTACAAATGTTTGTCACT
TAAATTTCAAGTTTCTGTTTTAATAGTTAACTGACTATAGATTGTTTTCTATGCCATGTA
TGTGCCACTTCTGAGAGTAGTAAATGACTCTTTGCTACATTTTAAAAGCAATTGTATTAG
TAAGAACTTTGTAAATAAATACCTAAAACCCAAGTGTAAAAAAAAAAAAAAAAAACTCGA
C
5' Read Nucleotide Sequence
>OriGene 5' read for NM_018098 unedited
GTATTTGTATACGACTCACTATGGGCGGCCGCGAATCGGCACGAGGGCCTAGAGATATCA
AGAAAACGGCACAAGGTTATTGGCACTTTTAGGAGTCCTCATGGCCAAACCCGACCCCCA
GCTTCTCTTAAGCATATTCACCTAATGCCTCTTTCTCAGATTAAGAAGGTATTGGACATA
AGAGAGACAGAAGATTGCCATAATGCTTTTGCCTTGCTTGTGAGGCCACCAACAGAGCAG
GCAAATGTGCTACTCAGTTTCCAGATGACATCAGATGAACTTCCAAAAGAAAACTGGCTA
AAGATGCTGTGTCGACATGTAGCTAACACCATTTGTAAAGCAGATGCTGAGAATCTTATT
TATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGT
AGAGCATCAAGAGCAATAAAAAAGACTTCAAAAAAGGTTACAAGAGCATTCTCTTTCTCC
AAAACTCCAAAAAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGA
AGAAGTCCTTCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTA
GCAGGTATCCCTTCTCCCTCCCTTGTCAGCCTTCCTTCCTTCTTTGAAAGGAGAAGTCAT
ACGTTAAGTAGATCTACAACTCATTTGATATGAAGCGTTACCCAAATCTTAAATTATAGN
AAATGTATAGACACCTCATACTCAAATAAGAAACTGACTTACATGGTACTTGGTATTAAC
ACTTGGTGAAAGCTGGAAGGAAGAAAATAACACTAAACTATGCTATNTGATTTTTCTTCC
TGAAAAGAGGAAGCTCACC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_018098 unedited
CGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTACACTTGGGTTTTAGGTATT
TATTTACAAAGTTCTTACTAATACAATTGCTTTTAAAATGTAGCAAAGAGTCATTTACTA
CTCTCAGAAGTGGCACATACATGGCATAGAAAACAATCTATAGTCAGTTAACTATTAAAA
CAGAAACTTGAAATTTAAGTGACAAACATTTGTAGCACTCCCTAAAGAAATAGGAAATAA
AAATGCATTTATCCATATGAACTTGATTATTCTGAATTACTGACTATAAAAAGGCTATTG
TGAAAGATATCACACTTTGAAACAGCAAATGAATTTTCAATTTTACATTTAATTATAAGA
CCACAATAAAAAGTTGAACATGCGCATATCTATGCATTTCACAGAAGATTAGTAAAACTG
ATGGCAACTTCAGAATTATTTCATGAAGGGTACAAACAGTCTTTACCACAATTTTCCCAT
GGTCTTATCCTTCAAAATAAAATTCCACACACTATCAAACTAAATCAAGATTTGCTAGTG
GATAAAATTACCATAAATATACCGTACTCTCTCTGAAACAGCTACAAACATCTTGTTTTT
GCAAAATATACAATGTTTCTCAATCTTTCTGTCCTTATCTCAATTTGCAAAAAATATTTT
GAAACAATCTCCTTTAAATGCTATTCCTTGTTAATGAGGGCAAATCTTTTAAAATCCACA
TGCTAGATCTTGAAAACGCTTGAGAAGACATAAACTGTGAAAGGAGTGGTTATTTAAATA
CTTCAAACTGCACATGTTGAACAGACCCACTATTTATCAAAATCTATGCCAGTTTCATCC
AGNATTATGAGTCATTTATCTCTGATAAATGAGCTGCCAATATCTATTCCATAAATCTTC
TGAACCTTACAATAGATTACAGCGGTTTGCCACCGCCCTAAATTATTACTT
Restriction Sites NotI-NotI |
ACCN NM_018098
Insert Size 1920 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018098.2, NP_060568.1
RefSeq Size 2309 bp
RefSeq ORF 549 bp
Locus ID 1894
UniProt ID Q9H8V3
Cytogenetics 3q26.31
Domains BRCT, RhoGEF
Protein Families Druggable Genome
Summary The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2017
Transcript Variant: This variant (3), as well as variant 2, encodes isoform b.
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Documents
Resources
"NM_018098" in other vectors (7)
SKU Description Size Price
RC211319 ECT2 (Myc-DDK-tagged)-Human epithelial cell transforming sequence 2 oncogene (ECT2) 10 ug
$1,212.00
RC211319L1 Lenti ORF clone of Human epithelial cell transforming sequence 2 oncogene (ECT2), Myc-DDK-tagged 10 ug
$1,512.00
RC211319L2 Lenti ORF clone of Human epithelial cell transforming sequence 2 oncogene (ECT2), mGFP tagged 10 ug
$1,512.00
RC211319L3 Lenti ORF clone of Human epithelial cell transforming sequence 2 oncogene (ECT2), Myc-DDK-tagged 10 ug
$1,512.00
RC211319L4 Lenti ORF clone of Human epithelial cell transforming sequence 2 oncogene (ECT2), mGFP tagged 10 ug
$1,512.00
RG211319 ECT2 (tGFP-tagged) - Human epithelial cell transforming sequence 2 oncogene (ECT2) 10 ug
$1,412.00
SC318356 ECT2 (untagged)-Human epithelial cell transforming sequence 2 oncogene (ECT2) 10 ug
$1,214.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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