SH3BP1 (NM_018957) Human Untagged Clone

SKU SC113307

SH3BP1 (untagged)-Human SH3-domain binding protein 1 (SH3BP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SH3BP1
• Synonyms: ARHGAP43
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018957, the custom clone sequence may differ by one or more nucleotides; ; ; ATGATGAAGAGGCAGCTGCACCGCATGCGGCAGCTGGCCCAGACGGGCAGCTTGGGACGCACCCCGGAGA; CCGCTGAGTTCCTGGGTGAGGACCTGCTGCAGGTAGAACAGCGGCTGGAGCCGGCCAAGCGGGCAGCCCA; CAACATCCACAAGCGGCTGCAGGCCTGTCTGCAGGGCCAGAGCGGGGCAGACATGGACAAGCGGGTGAAG; AAGCTTCCCCTCATGGCTCTGTCCACCACGATGGCTGAGAGCTTCAAGGAGCTGGACCCTGATTCCAGCA; TGGGGAAGGCCTTGGAGATGAGCTGTGCCATCCAGAATCAGCTGGCCCGCATCCTGGCCGAGTTTGAGAT; GACCCTGGAGAGGGACGTCCTGCAGCCACTCAGCAGGCTGAGTGAGGAGGAGCTGCCAGCCATCCTCAAA; CACAAGAAAAGCCTCCAGAAGCTCGTGTCCGACTGGAACACACTCAAGAGCAGGCTCAGTCAGGCAACCA; AGAATTCAGGCAGCAGTCAAGGCCTAGGAGGCAGCCCGGGTAGTCACAGCCATACGACCATGGCCAACAA; GGTGGAGACGCTGAAGGAGGAGGAGGAGGAGCTGAAGAGGAAAGTGGAGCAATGCAGGGACGAGTACTTG; GCTGACCTGTACCACTTTGTTACCAAGGAGGACTCCTATGCCAACTACTTCATTCGTCTCCTGGAGATTC; AGGCCGATTACCATCGCAGGTCACTGAGCTCGCTGGACACAGCCCTGGCTGAGCTGAGGGAGAACCACGG; CCAAGCAGACCACTCCCCTTCGATGACAGCCACCCACTTCCCCAGGGTGTATGGGGTGTCGCTGGCAACC; CACCTGCAAGAGCTGGGCCGGGAGATTGCCCTGCCCATCGAGGCCTGCGTCATGATGCTGCTTTCTGAGG; GCATGAAGGAAGAGGGTCTCTTCCGTCTGGCTGCTGGGGCCTCGGTGCTGAAGCGTCTCAAGCAGACAAT; GGCCTCGGACCCCCACAGCCTGGAGGAGTTCTGCTCCGACCCGCACGCTGTGGCAGGTGCCCTCAAGTCC; TATCTGCGGGAGCTGCCAGAGCCTCTGATGACCTTCGACCTCTATGATGACTGGATGAGGGCAGCCAGCC; TGAAGGAGCCAGGGGCCCGGCTGCAGGCCCTCCAAGAGGTGTGCAGCCGCCTACCCCCCGAGAACCTCAG; CAACCTCAGGTACCTGATGAAGTTCCTGGCACGGCTGGCCGAGGAGCAGGAGGTGAACAAGATGACACCC; AGCAACATCGCCATAGTCCTGGGACCCAACTTGCTGTGGCCACCTGAGAAAGAAGGGGACCAGGCCCAGC; TGGATGCAGCCTCCGTGTCTTCCATCCAGGTGGTGGGCGTCGTCGAGGCGCTGATCCAGAGCGCAGACAC; CCTCTTCCCTGGAGACATCAACTTCAACGTGTCAGGCCTCTTCTCAGCTGTTACCCTCCAGGACACAGTC; AGTGACAGGCTGGCCTCTGAGGAACTTCCGTCCACTGCCGTGCCCACCCCAGCCACCACCCCGGCTCCGG; CTCCGGCTCCAGCTCCAGCTCCGGCCCCAGCCTTGGCTTCAGCAGCTACCAAGGAAAGGACAGAGTCTGA; GGTGCCTCCCAGACCAGCCTCCCCCAAGGTCACCAGGAGTCCCCCGGAGACAGCTGCCCCAGTGGAGGAC; ATGGCTCGGAGGACCAAGCGCCCGGCGCCAGCCCGGCCCACCATGCCGCCCCCCCAGGTCTCCGGCTCCC; GCTCCTCCCCTCCAGCCCCGCCCTTGCCCCCTGGCTCTGGCAGCCCTGGGACCCCCCAAGCCCTGCCCCG; ACGTCTGGTTGGCAGCAGCCTCCGAGCCCCCACAGTGCCACCCCCGTTACCCCCCACACCCCCTCAGCCT; GCCCGGCGCCAAAGCCGGCGTTCACCAGCCTCCCCCAGCCCGGCCTCCCCAGGTCCAGCCTCCCCCAGCC; CAGTCTCTTTGAGTAACCCTGCACAGGTGGACCTGGGGGCTGCCACAGCAGAGGGAGGAGCCCCTGAGGC; TATCAGTGGGGTCCCCACTCCCCCAGCTATCCCCCCTCAGCCCCGCCCCAGGAGCCTTGCCTCAGAGACC; AACTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018957 unedited; NGTTCAGATATTTGTATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAGAG; GCAGGCTGGACCGGGGGCTCCCCGGGCCCGCGACCCCCGCCGTGACCCCGCAGCCCCCAG; CTCGCCCCCAAGATGATGAAGAGGCAGCTGCACCGCATGCGGCAGCTGGCCCAGACGGGC; AGCTTGGGACGCACCCCGGAGACCGCTGAGTTCCTGGGTGAGGACCTGCTGCAGGTAGAA; CAGCGGCTGGAGCCGGCCAAGCGGGCAGCCCACAACATCCACAAGCGGCTGCAGGCCTGT; CTGCAGGGCCAGAGCGGGGCAGACATGGACAAGCGGGTGAAGAAGCTTCCCCTCATGGCT; CTGTCCACCACGATGGCTGAGAGCTTCAAGGAGCTGGACCCTGATTCCAGCATGGGGAAG; GCCTTGGAGATGAGCTGTGCCATCCAGAATCAGCTGGCCCGCATCCTGGCCGAGTTTGAG; ATGACCCTGGAGAGGGACGTCCTGCAGCCACTCAGCAGGCTGAGTGAGGAGGAGCTGCCA; GCCATCCTCAAACACAAGAAAAGCCTCCAGAAGCTCGTGTCCGACTGGAACACACTCAAG; AGCAGGCTCAGTCAGGCAACCAAGAATTCAGGCAGCAGTCAAGGCCTAGGAGGCAGCCCG; GGTAGTCACAGCCATACGACCATGGNCCAACAGGTGGAGACGCTGAAAGGAGAGGAGGGA; GAGCTGAANAGGAAAGTGGAGCAATGCAGGGACGAGTACTTGGCTGACCTGTACCACTTT; GNTACCAAGGAGGACTNCTATGCCAACTACTTCATTCGTCTCCTGGAGATTCAGGCCGAT; ACCCATCGCAGTCACTGAGCTCGCTGGACCCGCCCTGCCTGAGCTGAGGGAGACCACGNN; CAGCCAGACACTCCCCTTCGTGACAGACACCACTCCCAGGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018957 unedited; GGGGGGGCNNTGGCNNNACCCCNNNNNTTTTNNNNNNNNGTTTTTGACTTAGACAGNGGN; CCGCNTACTANGANCGNGTTTTTTTTTTTTTTTTTNTCCGTTTTGTCCAAAATATTTATT; TACCAGCCTTACAAAAAAATGTCGGCAAAAAAAAAATCATCCCGTAGAAGCAGGCAAACC; TCTCCTTCCTTCCGGTGGCTCCCCTAGGACCTGCCGGAGAGTGGAGAGTCCGGTGGGGGG; GTCCCCAAGCCCAGGGTGGACGAGGAAAAGGTCAGGAAATAGAGGATTGTCCTGAGCCCT; CCTGGCCATGGGGGCCGACCCAGTGGGCACTGAGGCACTTGTGGGCAAAGGCTGGAGGCC; CATGCCCCTTTGTGGGGGGCGAGAGCTGTCCTGGGAGGGCCAGGTGGGGAGGGAGGGGGT; GCTGAGGGCTGCTTAGGGAGAAACCAGCCACTCAGTTGGTCTCTGAGGCAAGGCTCCTGG; GGCGGGGCTGAGGGGGGATAGCTGGGGGAGTGGGGACCCCACTGATAGCCTCAGGGGCTC; CTCCCTCTGCTGTGGCAGCCCCCAGGTCCACCTGTGCAGGGTTACTCAAAGAGACTGGGC; TGGGGGAGGCTGGACCTGGGGAGGCCGGGCTGGGGGAGGCTGGTGAACGCCGGCTTTGGC; GCCGGGCAGGCCTGAGGGGTGTGGGGGGTAACGGGGGTGGCACTGTGGGGGCTCGGAGGC; TGCTGGCAACCAAACGTTGGGGCCGGGCTTGGGGGGTCCCCAGGGCTGCCAGAGCCAGGG; GCCAAGGGGGGGCTTGGAGGGGGATAACCGGTAACCCGGAAACCTGGGGGGGCCGGCTTG; GTGGGCCCGGTCTGGCCCCCGGCCGCTGGGTCCTCCCAGACCATGGCCTCCCATGGGGCA; AGCTGTTTCCGGGGGACCCCTGGTGACCTTGGGGGAGGTTTGTCCTGGAAGN;
• Restriction Sites: NotI-NotI
• ACCN: NM_018957
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018957.2, NP_061830.2
• RefSeq Size: 2592 bp
• RefSeq ORF: 2592 bp
• Locus ID: 23616
• UniProt ID: Q9Y3L3
• Cytogenetics: 22q13.1
• Domains: RhoGAP
• Summary: This gene encodes a member of the Rho GTPase activating protein (RhoGAP) family. The encoded protein regulates Rac signaling and plays a role in cytoskeletal dynamics, cell motility and epithelial junction formation. This protein's association with the exocyst complex, which tethers secretory vesicles to the plasma membrane, has been demonstrated to be important in cell motility. In a distinct complex, this protein has been shown to regulate epithelial junction formation and morphogenesis. By interacting with the Plexin-D1 cell surface receptor, this protein mediates changes in the cytoskeleton in response to semaphorin binding. This protein may promote metastasis in human liver cancer cells and tissues. [provided by RefSeq, Mar 2017]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).