PAK6 (NM_020168) Human Untagged Clone

CAT#: SC113203

PAK6 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 6 (PAK6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAK6
• Synonyms: PAK5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113203 sequence for NM_020168 edited (data generated by NextGen Sequencing)
  ATGTTCCGCAAGAAAAAGAAGAAACGCCCTGAGATCTCAGCGCCACAGAACTTCCAGCAC
  CGTGTCCACACCTCCTTCGACCCCAAAGAAGGCAAGTTTGTGGGCCTCCCCCCACAATGG
  CAGAACATCCTGGACACACTGCGGCGCCCCAAGCCCGTGGTGGACCCTTCGCGAATCACA
  CGGGTGCAGCTCCAGCCCATGAAGACAGTGGTGCGGGGCAGCGCGATGCCTGTGGATGGC
  TACATCTCAGGGCTGCTCAACGACATCCAGAAGTTGTCAGTCATCAGCTCCAACACCCTG
  CGTGGCCGCAGCCCCACCAGCCGGCGGCGGGCACAGTCCCTGGGGCTGCTGGGGGATGAG
  CACTGGGCCACCGACCCAGACATGTACCTCCAGAGCCCCCAGTCTGAGCGCACTGACCCC
  CACGGCCTCTACCTCAGCTGCAACGGGGGCACACCAGCAGGCCACAAGCAGATGCCGTGG
  CCCGAGCCACAGAGCCCACGGGTCCTGCCCAATGGGCTGGCTGCAAAGGCACAGTCCCTG
  GGCCCCGCCGAGTTTCAGGGTGCCTCGCAGCGCTGTCTGCAGCTGGGTGCCTGCCTGCAG
  AGCTCCCCACCAGGAGCCTCGCCCCCCACGGGCACCAATAGGCATGGAATGAAGGCTGCC
  AAGCATGGCTCTGAGGAGGCCCGGCCACAGTCCTGCCTGGTGGGCTCAGCCACAGGCAGG
  CCAGGTGGGGAAGGCAGCCCTAGCCCTAAGACCCGGGAGAGCAGCCTGAAGCGCAGGCTA
  TTCCGAAGCATGTTCCTGTCCACTGCTGCCACAGCCCCTCCAAGCAGCAGCAAGCCAGGC
  CCTCCACCACAGAGCAAGCCCAACTCCTCTTTCCGACCGCCGCAGAAAGACAACCCCCCA
  AGCCTGGTGGCCAAGGCCCAGTCCTTGCCCTCGGACCAGCCGGTGGGGACCTTCAGCCCT
  CTGACCACTTCGGATACCAGCAGCCCCCAGAAGTCCCTCCGCACAGCCCCGGCCACAGGC
  CAGCTTCCAGGCCGGTCTTCCCCAGCGGGATCCCCCCGCACCTGGCACGCCCAGATCAGC
  ACCAGCAACCTGTACCTGCCCCAGGACCCCACGGTTGCCAAGGGTGCCCTGGCTGGTGAG
  GACACAGGTGTTGTGACACATGAGCAGTTCAAGGCTGCGCTCAGGATGGTGGTGGACCAG
  GGTGACCCCCGGCTGCTGCTGGACAGCTACGTGAAGATTGGCGAGGGCTCCACCGGCATC
  GTCTGCTTGGCCCGGGAGAAGCACTCGGGCCGCCAGGTGGCCGTCAAGATGATGGACCTC
  AGGAAGCAGCAGCGCAGGGAGCTGCTCTTCAACGAGGTGGTGATCATGCGGGACTACCAG
  CACTTCAACGTGGTGGAGATGTACAAGAGCTACCTGGTGGGCGAGGAGCTGTGGGTGCTC
  ATGGAGTTCCTGCAGGGAGGAGCCCTCACAGACATCGTCTCCCAAGTCAGGCTGAATGAG
  GAGCAGATTGCCACTGTGTGTGAGGCTGTGCTGCAGGCCCTGGCCTACCTGCATGCTCAG
  GGTGTCATCCACCGGGACATCAAGAGTGACTCCATCCTGCTGACCCTCGATGGCAGGGTG
  AAGCTCTCGGACTTCGGATTCTGTGCTCAGATCAGCAAAGACGTCCCTAAGAGGAAGTCC
  CTGGTGGGAACCCCCTACTGGATGGCTCCTGAAGTGATCTCCAGGTCTTTGTATGCCACT
  GAGGTGGATATCTGGTCTCTGGGCATCATGGTGATTGAGATGGTAGATGGGGAGCCACCG
  TACTTCAGTGACTCCCCAGTGCAAGCCATGAAGAGGCTCCGGGACAGCCCCCCACCCAAG
  CTGAAAAACTCTCACAAGGTCTCCCCAGTGCTGCGAGACTTCCTGGAGCGGATGCTGGTG
  CGGGACCCCCAAGAGAGAGCCACAGCCCAGGAGCTCCTAGACCACCCCTTCCTGCTGCAG
  ACAGGGCTACCTGAGTGCCTGGTGCCCCTGATCCAGCTCTACCGAAAGCAGACCTCCACC
  TGCTGA
  
  Clone variation with respect to NM_020168.4
  249 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020168 unedited
  CACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCATCTGATTATTT
  TGGGAGCTGGAAACTTGGAGCTGCACCTGAGTCCCGCCCCTTCTAGCTCTCCCCTCCCTA
  CCTTGGGCTCCAGGAAGATGGGACTTGCTGTGAGTCTGCTGCCACCCCCTAAAGATATGG
  AAGACGCTGTGGGGGCCAGAAGTGCCCGGGGGGCTGTGGCAGCAGGCAGAGTGCAATAGC
  AGATATGGTGGTCAGGCTGCCCGTGTGTGTCCTCTGGAGGTGTTGGGACAGAAGGGCAGT
  CTTGTCCGAGCTGACTGGAGTCCTCCCGGGCTGGCTCTGAACTCATCTCCCACGGGGATG
  TTTCGGTAAAGGAGTGGCTTCTGGGGTCGGAGTGGCATTTGGAGAGCGAGGCTGGATTGG
  CTTANGCTGGCCTGGGCAGGGAGTGCCGCTTCCTGGGCTAGAGACAAGCACCAGCCTGCA
  GTGGAGAACGCAGGACCCCGCTGCCCAGAAGGAGCAGCCACGGCCTGCGGAGGACTGGCC
  CAGCAAGGTCCCAGGTCTTCCCTCTCCTCAGCGCCTAAGAGAGAGGCCCAGTGCGGGTGA
  GGAGTCGCGAGGAAGAGGCGGAAGGCGCCGGAAGGCACCATGTTCCGCAAGAAAAAGAAG
  AAACGCCCTGAGATCTCAGCGCCACAGAACTTCCAGCACCGTGTCCACACCTCCTTCGAC
  CCCAAAGAAGGCAAGTTTGTGGGCCTCCCCCCACAATGGCAGAACATCCTGGACACACTG
  CGGCGCCCCAAGCCGTGGTGGACCCTTCGCGATCACACGGGTGCAGCTCCAGCCCATGAA
  GACAGTGGTGCGGNGCANCGCGATGCCTGTGGATGGCTACATCTCAGGGCTGCTCACGAC
  TNCCAGAAGTGTCAGTCATCAGCTCACACCCTGCGTGGCCGCAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020168 unedited
  NTTTTTTGACTCTGCCCCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTT
  TGGCTTTGAACACTCAATTTAATTTAAAACAGGCACAAGTGCAAACAATTCACAAAAATT
  TCTAGGGAAGATGCTTTTGTTTTGAAAACTCTGACCCTTAAAAAAAAGTCCTTGCAATTT
  CTTTGCCCCCAGGTAGGTCACTAGGGAGCAGAAGAATCTAAAAATATTATCTAGATAGAA
  AGGGTCCAGACACCTGAAGTTCTTTCCTGGAATTCCATCTCACAGCAGCCCTGAAGTGGG
  GCAGGGCCGAGGAGGACAAGGAGACAGCAGTCTGTGGAGGCAGCTGGGGCAGATGGTTTG
  CACACATGGATGCCTGTGACCTTGACATGATGGAAACCCGGAGGTGGTTCGCCTTCTCTC
  CCAGATAGGGTTCTGAAGAGAACAATGGGTCCCTGATGACCTGGCTATTGAGATAAACTT
  GACAGGTAACACAGCCGCCAATCCTTACATTCCTGTCTCTGGCCCTCACGATGCACAGTA
  CAGGAGAAGCTCAAAACCCTCCTGAGCCTCCAGGAGTGCTAGACTTACCATGGACCACTC
  CACTGGCCACGTACACACCGGAGTGGCAGAGGCGGCAACCCCGCTCAACAAAGCCGGTTC
  CTTTATGCCAATACTTATTGGCCAAAACGGGCCCTTCTCTCCGAAACCGGTTGCGGGCGA
  CCCTGCAGAGACCCGGGTTGTTTTGGGGGGTTTTCTTTCCCTTACAGCTCTTTACTACCC
  CCCCACCCCCCCCTACCTTGTCTCTCTTTTCGCAACCGCCCACCCCTTTTCCCCCCCTCC
  CTCTCCTTCCCCCTCTCCCCAAAATGCGCCCCCCCTCCCCTCCCTCCCCTCCCTTCCTGT
  CTTCTCCCCCCCCACCCCCGCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020168
• Insert Size: 4470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020168.3, NP_064553.1
• RefSeq Size: 3889 bp
• RefSeq ORF: 2046 bp
• Locus ID: 56924
• UniProt ID: Q9NQU5
• Cytogenetics: 15q15.1
• Domains: PBD, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: This gene encodes a member of a family of p21-stimulated serine/threonine protein kinases, which contain an amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. These kinases function in a number of cellular processes, including cytoskeleton rearrangement, apoptosis, and the mitogen-activated protein (MAP) kinase signaling pathway. The protein encoded by this gene interacts with androgen receptor (AR) and translocates to the nucleus, where it is involved in transcriptional regulation. Changes in expression of this gene have been linked to prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 4 encode the same isoform (1).