SCUBE2 (NM_020974) Human Untagged Clone

SKU SC113056

SCUBE2 (untagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SCUBE2
• Synonyms: CEGB1; CEGF1; CEGP1; scube/You
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113056 sequence for NM_020974 edited (data generated by NextGen Sequencing); ATGGGGGTCGCGGGCCGCAACCGTCCCGGGGCGGCCTGGGCGGTGCTGCTGCTGCTGCTG; CTGCTGCCGCCACTGCTGCTGCTGGCGGGGGCCGTCCCGCCGGGTCGGGGCCGTGCCGCG; GGGCCGCAGGAGGATGTAGATGAGTGTGCCCAAGGGCTAGATGACTGCCATGCCGACGCC; CTGTGTCAGAACACACCCACCTCCTACAAGTGCTCCTGCAAGCCTGGCTACCAAGGGGAA; GGCAGGCAGTGTGAGGACATCGATGAATGTGGAAATGAGCTCAATGGAGGCTGTGTCCAT; GACTGTTTGAATATTCCAGGCAATTATCGTTGCACTTGTTTTGATGGCTTCATGTTGGCT; CATGACGGTCATAATTGTCTTGATGTGGACGAGTGCCTGGAGAACAATGGCGGCTGCCAG; CATACCTGTGTCAACGTCATGGGGAGCTATGAGTGCTGCTGCAAGGAGGGGTTTTTCCTG; AGTGACAATCAGCACACCTGCATTCACCGCTCGGAAGAGGGCCTGAGCTGCATGAATAAG; GATCACGGCTGTAGTCACATCTGCAAGGAGGCCCCAAGGGGCAGCGTCGCCTGTGAGTGC; AGGCCTGGTTTTGAGCTGGCCAAGAACCAGAGAGACTGCATCTTGACCTGTAACCATGGG; AACGGTGGGTGCCAGCACTCCTGTGACGATACAGCCGATGGCCCAGAGTGCAGCTGCCAT; CCACAGTACAAGATGCACACAGATGGGAGGAGCTGCCTTGAGCGAGAGGACACTGTCCTG; GAGGTGACAGAGAGCAACACCACATCAGTGGTGGATGGGGATAAACGGGTGAAACGGCGG; CTGCTCATGGAAACGTGTGCTGTCAACAATGGAGGCTGTGACCGCACCTGTAAGGATACT; TCGACAGGTGTCCACTGCAGTTGTCCTGTTGGATTCACTCTCCAGTTGGATGGGAAGACA; TGTAAAGATATTGATGAGTGCCAGACCCGCAATGGAGGTTGTGATCATTTCTGCAAAAAC; ATCGTGGGCAGTTTTGACTGCGGCTGCAAGAAAGGATTTAAATTATTAACAGATGAGAAG; TCTTGCCAAGATGTGGATGAGTGCTCTTTGGATAGGACCTGTGACCACAGCTGCATCAAC; CACCCTGGCACATTTGCTTGTGCTTGCAACCGAGGGTACACCCTGTATGGCTTCACCCAC; TGTGGAGACACCAATGAGTGCAGCATCAACAACGGAGGCTGTCAGCAGGTCTGTGTGAAC; ACAGTGGGCAGCTATGAATGCCAGTGCCACCCTGGGTACAAGCTCCACTGGAATAAAAAA; GACTGTGTGGAAGTGAAGGGGCTCCTGCCCACAAGTGTGTCACCCCGTGTGTCCCTGCAC; TGCGGTAAGAGTGGTGGAGGAGACGGGTGCTTCCTCAGATGTCACTCTGGCATTCACCTC; TCTTCAGGACTGCAAGGGGCCTACTCTGTCACCTGTGGCTCTTCCTCTCCTCTCAGGAAC; AAACAACAAAAATCAAATGACTCTGCTTTTGGGGATGTCACCACCATCAGGACAAGTGTA; ACCTTTAAGCTAAATGAAGGCAAGTGTAGTTTGAAAAATGCTGAGCTGTTTCCCGAGGGT; CTGCGACCAGCACTACCAGAGAAGCACAGCTCAGTAAAAGAGAGCTTCCGCTACGTAAAC; CTTACATGCAGCTCTGGCAAGCAAGTCCCAGGAGCCCCTGGCCGACCAAGCACCCCTAAG; GAAATGTTTATCACTGTTGAGTTTGAGCTTGAAACTAACCAAAAGGAGGTGACAGCTTCT; TGTGACCTGAGCTGCATCGTAAAGCGAACCGAGAAGCGGCTCCGTAAAGCCATCCGCACG; CTCAGAAAGGCCGTCCACAGGGAGCAGTTTCACCTCCAGCTCTCAGGCATGAACCTCGAC; GTGGCTAAAAAGCCTCCCAGAACATCTGAACGCCAGGCAGAGTCCTGTGGAGTGGGCCAG; GGTCATGCAGAAAACCAATGTGGTCTGTGTCAACCTGGTGAATATTCTGCAGATGGCTTT; GCACCTTGCCAGCTCTGTGCCCTGGGCACGTTCCAGCCTGAAGCTGGTCGAACTTCCTGC; TTCCCCTGTGGAGGAGGCCTTGCCACCAAACATCAGGGAGCTACTTCCTTTCAGGACTGT; GAAACCAGAGTTCAATGTTCACCTGGACATTTCTACAACACCACCACTCACCGATGTATT; CGTTGCCCAGTGGGAACATACCAGCCTGAATTTGGAAAAAATAATTGTGTTTCTTGCCCA; GGAAATACTACGACTGACTTTGATGGCTCCACAAACATAACCCAGTGTAAAAACAGAAGA; TGTGGAGGGGAGCTGGGAGATTTCACTGGGTACATTGAATCCCCAAACTACCCAGGCAAT; TACCCAGCCAACACCGAGTGTACGTGGACCATCAACCCACCCCCCAAGCGCCGCATCCTG; ATCGTGGTCCCTGAGATCTTCCTGCCCATAGAGGACGACTGTGGGGACTATCTGGTGATG; CGGAAAACCTCTTCATCCAATTCTGTGACAACATATGAAACCTGCCAGACCTACGAACGC; CCCATCGCCTTCACCTCCAGGTCAAAGAAGCTGTGGATTCAGTTCAAGTCCAATGAAGGG; AACAGCGCTAGAGGGTTCCAGGTCCCATACGTGACATATGATGAGGACTACCAGGAACTC; ATTGAAGACATAGTTCGAGATGGCAGGCTCTATGCATCTGAGAACCATCAGGAAATACTT; AAGGATAAGAAACTTATCAAGGCTCTGTTTGATGTCCTGGCCCATCCCCAGAACTATTTC; AAGTACACAGCCCAGGAGTCCCGAGAGATGTTTCCAAGATCGTTCATCCGATTGCTACGT; TCCAAAGTGTCCAGGTTTTTGAGACCTTACAAATGA; ; Clone variation with respect to NM_020974.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020974 unedited; GGATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATCGGCACGAGGCCGCGCACAC; CTCCCCGCGCCGCCGCCGCCACCGCCCGCACTCCGCCGCCTCTGCCCGCAACCGCTGAGC; CATCCATGGGGGTCGCGGGCCGCAACCGTCCCGGGGCGGCCTGGGCGGTGCTGCTGCTGC; TGCTGCTGCTGCCGCCACTGCTGCTGCTGGCGGGGGCCGTCCCGCCGGGTCGGGGCCGTG; CCGCGGGGCCGCAGGAGGATGTAGATGAGTGTGCCCAAGGGCTAGATGACTGCCATGCCG; ACGCCCTGTGTCAGAACACACCCACCTCCTACAAGTGCTCCTGCAAGCCTGGCTACCAAG; GGGAAGGCAGGCAGTGTGAGGACATCGATGAATGTGGAAATGAGCTCAATGGAGGCTGTG; TCCATGACTGTTTGAATATTCCAGGCAATTATCGTTGCACTTGTTTTGATGGCTTCATGT; TGGCTCATGACGGTCATAATTGTCTTGATGTGGACGAGTGCCTGGAGAACAATGGCGGCT; GCCAGCATACCTGTGTCAACGTCATGGGGAGCTATGAGTGCTGCTGCAAGGAGGGGTTTT; TCCTGAGTGACAATCAGCACACCTGCATTCACCGCTNCGAAGAGGCCTGAGCTGCATGAA; TAAGGATCACGGCTGTAGTCACATCTGCAAGGANGGCCCCAAGGNGCAGCGTCGCCTGTG; AGTGCANGCCTGGNTTTGAGCTGCCCAGAACAGAGAGACTGCATCTTGACCTGTAACCAT; GGGAACGGTGGNTGCCAGCACTNCTGTGACATACAGCCGATGGCCCAGATGCAGCTGCCA; TCCCAGTACAGGATGCACACGATGGGGAGAGCTGGCTTGACGAGAAGACACTGTCCTGNN; AGTGACGAAAAGCACACCCATCAATGGTGGATGGGATAACGGGTGAAACGCGCTGTCATG; AACCNTGGCTGCACAATGAGCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020974 unedited; AGCTATGCCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAATTTACCA; AAATATCTGTATTATCTATAAAAATTGAACTCTAATGAGTCACTGATACGGGAGGCAGCA; ATACCCGACTGTGCTGACATGCAGAAGGAAGACAGCTCTGTCCCACCAACCCTATAGCAG; AACATTTGTATTGAGTGGCACGTGGGCTGAGTCATTTGTAAGGTCTCAAAAACCTGGACA; CTTTGGAACGTAGCAATCGGATGAACGATCTTGGAAACATCTCTCGGGACTCCTGGGCTG; TGTACTTGAAATAGTTCTGGGGATGGGCCAGGACATCAAACAGAGCCTTGATAAGTTTCT; TATCCTTAAGTATTTCCTGATGGTTCTCAGATGCATAGAGCCTGCCATCTCGAACTATGT; CTTCAATGAGTTCCTGGTAGTCCTCATCATATGTCACGTATGGGACCTGGAACCCTCTAG; CGCTGTTCCCTTCATTGGACTTGAACTGAATCCACAGCTTCTTTGACCTGGAGGTGAAGG; CGATGGGGCGTTCGTAGGTCTGGCAGGTTTCATATGTTGTCACAGAATTGGATGAAGAGG; TTTTCCGCATCACCAGATAGTCCCCACAGTCGTCCTCTATGGGCAGGAAGATCTCANGGA; CCACGATCAGGATGCGGCGCTTGGGGGGTGGGTTGATGGTCCACGTACACCNTCGTGNTG; GCTGGGNTAATTGCCTGGGGTAGTTGGGGGGATTCAATGTACCCAGTGAAAATCTCCAGC; TNCCCTNCACATCNTCTGTTTTTACACTGGGGGTATGTTGTGGGAGCCATCAAAGTCAGT; CGTAGTATTTCCTGGGCAAGAAACACATTATTTTTTCCAAATCAGGGCTGTATGTTTCCA; CTGGGCAACGAATACATCGTGANTGGGGGTGTTGTAAAATGTTCAGNGAACATTGACCTT; GGGTTCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_020974
• Insert Size: 3850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020974.1, NP_066025.1
• RefSeq Size: 3737 bp
• RefSeq ORF: 3000 bp
• Locus ID: 57758
• UniProt ID: Q9NQ36
• Cytogenetics: 11p15.4
• Domains: CUB, EGF, EGF_CA
• Protein Families: Transmembrane
• Summary: Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane (PubMed:22902404, PubMed:22677548). Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface (PubMed:24522195). Synergizes with DISP1 to increase SHH secretion (PubMed:22902404). Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis (PubMed:27834687).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).