TRIB3 (NM_021158) Human Untagged Clone

SKU SC112991

TRIB3 (untagged)-Human tribbles homolog 3 (Drosophila) (TRIB3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRIB3
• Synonyms: C20orf97; NIPK; SINK; SKIP3; TRB3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_021158 edited; ATGCGAGCCACCCCTCTGGCTGCTCCTGCGGGTTCCCTGTCCAGGAAGAAGCGGTTGGAG; TTGGATGACAACTTAGATACCGAGCGTCCCGTCCAGAAACGAGCTCGAAGTGGGCCCCAG; CCCAGACTGCCCCCCTGCCTGTTGCCCCTGAGCCCACCTACTGCTCCAGATCGTGCAACT; GCTGTGGCCACTGCCTCCCGTCTTGGGCCCTATGTCCTCCTGGAGCCCGAGGAGGGCGGG; CGGGCCTACCAGGCCCTGCACTGCCCTACAGGCACTGAGTATACCTGCAAGGTGTACCCC; GTCCAGGAAGCCCTGGCCGTGCTGGAGCCCTACGCGCGGCTGCCCCCGCACAAGCATGTG; GCTCGGCCCACTGAGGTCCTGGCTGGTACCCAGCTCCTCTACGCCTTTTTCACTCGGACC; CATGGGGACATGCACAGCCTGGTGCGAAGCCGCCACCGTATCCCTGAGCCTGAGGCTGCC; GTGCTCTTCCGCCAGATGGCCACCGCCCTGGCGCACTGTCACCAGCACGGTCTGGTCCTG; CGTGATCTCAAGCTGTGTCGCTTTGTCTTCGCTGACCGTGAGAGGAAGAAGCTGGTGCTG; GAGAACCTGGAGGACTCCTGCGTGCTGACTGGGCCAGATGATTCCCTGTGGGACAAGCAC; GCGTGCCCAGCCTACGTGGGACCTGAGATACTCAGCTCACGGGCCTCATACTCGGGCAAG; GCAGCCGATGTCTGGAGCCTGGGCGTGGCGCTCTTCACCATGCTGGCCGGCCACTACCCC; TTCCAGGACTCGGAGCCTGTCCTGCTCTTCGGCAAGATCCGCCGCGGGGCCTACGCCTTG; CCTGCAGGCCTCTCGGCCCCTGCCCGCTGTCTGGTTCGCTGCCTCCTTCGTCGGGAGCCA; GCTGAACGGCTCACAGCCACAGGCATCCTCCTGCACCCCTGGCTGCGACAGGACCCGATG; CCCTTAGCTCCAACCCGATCCCATCTCTGGGAGGCTGCCCAGGTGGTCCCTGATGGACTG; GGGCTGGACGAAGCCAGGGAAGAGGAGGGAGACAGAGAAGTGGTTCTGTATGGCTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021158 unedited; NGGTTCACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTGCGAT; CCCGGGCCCGAGGGCATCAGACGGCGGCTGATTAGCTCCGGTTTGCATCACCCGGACCGG; GGGATTAGCTCCGGTTTGCATCACCCGAACCGGGGGATTAGCTCCGGTTTGCATCACCCG; GACCGGGGGCCGGGCGCGCACGAGACTCGCAGCGGAAGTGGAGGCGGCTCCGCGTGCGTC; CGCTGCTAGGACCCGGGCAGGGCTGGAGCTGGGCTGGGATCCCGAGCTCGGCAGCAGCGC; AGCGGGCCGGCCCACCTGCTGGTGCCCTGGAGGCTCTGAGCCCCGGCGGCGCCCGGGCCC; ACGCGGAACGACGGGGCGAGATGCGAGCCACCCCTCTGGCTGCTCCTGCGGGTTCCCTGT; CCAGGAAGAAGCGGTTGGAGTTGGATGACAACTTAGATACCGAGCGTCCCGTCCAGAAAC; GAGCTCGAAGTGGGCCCCAGCCCAGACTGCCCCCCTGCCTGTTGCCCCTGAGCCCACCTA; CTGCTCCAGATCGTGCAACTGCTGTGGCCACTGCCTCCCGTCTTGGGCCCTATGTCCTCC; TGGAGCCCGAGGAGGGGCGGGCGGGCCTACCAGGCCCTGCACTGCCCTACAGGCACTGAG; TATACCTGCAAGGTGTACCCCGTCCAGGAAGCCCTGGCCGTGCTGGAGCCCTACGCGCGG; CTGCCCCCGCACAAGCATGTGGCTCGGCCCACTGAGGTCCTGGCTGGTACCCAGCTCCTC; TACGCCTTTTTCACTCGGACCCATGGNGACATGCACAGCCTGGTGCGAAGCCGCCACCGT; ATCCCTGAGCCTGAGGCTGCCGTGCTCTTCCGCCAGATGCCACCGCCCTGGCGCACTGTA; CCAGCACCGTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021158 unedited; AAGGCCGCACGTGAGAGTCGAGTTTTTTTCTTTTTTTTTTATTTCATAATTCTCCTTTAT; TAGGCACAGGTAAACATACATACTCATGGTATCCAAAACCTAGAGTATGGACCTGGGATT; GTGGACCCCAAGTGTCCCCAGAAGAGTCCCACCTGGGACTTTCCAGGTGGCCACAGGACA; GACCCTGCCTAATCCTGTCCCTCAACCTTGGTGCTCAGGTCAGAAGCCCCATGGTTGACA; GGCCTGGACCCTCATTCCAGAACAGTCTTGAGTTAGACAAGAACTAGCCTCATAGTTTGG; ATTCTTATCTCTGGCCCAAATCCCAGGCTTAGGCCTGGAAGGAGAATCTCTTAATCAAGA; GGACAGAGATGCTGGGAACACAGTTCCCAGAGTGAGCTGGGCTTGGGTCCTGAGTTGCAG; GGGAGAGGCCTAGAGCCTTTGGCACAGGGATACCTCCCTTTCTCTGAAAAGGTACAAGAT; ACAAAGGCAGCTGGTTTGTTTGTGAAAGGGATTGTCCTTGGACAGAGGTGCCTGAGCACC; AGCATCAGTGGAGGCCGACACTGGTACAAAGTGTACCCAGTGTAGACAGTGACCTATGGT; CATGGAAAAGTGCTGTCACCTCCAGGATGCCAGCTTGTCCTCTCTGCGAGCAGGGAACAT; TGGCTCCTTTGCTCACTGCTGGGACTCCCAGCACCGGAAACAGGCCTTGGCACCTGATAA; CACCCCAAGCAAGAACTGGCTGTGGTGTGGGACAAACCAGATGGGTCACCGGACACCCCC; ACCAAGGCTCCTGCCCTTNTCCCCTTCTGGAAGGCCCTCGAAGTTTCGCCTCACCTCAAA; GCCCGGAGAAGGGCTGGAGCCTCCCCCCAACCTCACCCCCTGTGGCCCCTAGCCTGCACT; ACGCGTGTCCTAACATAAAAACCCTTTCCNTGGCCCCTCCCCTTCCTGGTTCGGACCACC; CCAAACATAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_021158
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021158.3, NP_066981.2
• RefSeq Size: 2469 bp
• RefSeq ORF: 1077 bp
• Locus ID: 57761
• UniProt ID: Q96RU7
• Cytogenetics: 20p13
• Domains: pkinase, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Summary: The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]; Transcript Variant: This variant (1, also known as 1A) represents the longest transcript and encode the predominant isoform (1).