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MRPL23 (NM_021134) Human Untagged Clone

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SKU
SC112979
MRPL23 (untagged)-Human mitochondrial ribosomal protein L23 (MRPL23), nuclear gene encoding mitochondrial protein
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MRPL23
Synonyms L23MRP; RPL23; RPL23L
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC112979 sequence for NM_021134 edited (data generated by NextGen Sequencing)
ATGGCGCGGAATGTGGTGTACCCCCTGTACCGGCTGGGTGGCCCACAACTTCGGGTGTTC
CGAACCAACTTCTTCATTCAGCTGGTGCGGCCCGGTGTGGCCCAGCCCGAGGACACCGTG
CAGTTCCGGATCCCCATGGAAATGACAAGGGTGGACCTCAGGAATTACCTCGAGGGCATC
TATAACGTGCCCGTGGCTGCTGTGCGGACACGGGTGCAGCATGGCTCTAACAAGAGAAGA
GATCACAGAAACGTGAGGATCAAGAAGCCGGACTACAAGGTCGCCTACGTGCAGCTGGCC
CATGGACAGACCTTCACGTTCCCAGATCTGTTTCCCGAGAAAGACGAGAGCCCTGAAGGC
AGCGCTGCCGACGACCTCTACAGCATGCTCGAGGAGGAGAGGCAGCAGAGGCAGAGCAGC
GACCCGCGGCGGGGCGGCGTCCCCAGCTGGTTCGGGCTGTGA

Clone variation with respect to NM_021134.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_021134 unedited
ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCCGGNAGCGCGCGTGGCC
GCCATGGCGCGGAATGTGGTGTACCCCCTGTACCGGCTGGGTGGCCCACAACTTCGGGTG
TTCCGAACCAACTTCTTCATTCAGCTGGTGCGGCCCGGTGTGGCCCAGCCCGAGGACACC
GTGCAGTTCCGGATCCCCATGGAAATGACAAGGGTGGACCTCAGGAATTACCTCGAGGGC
ATCTATAACGTGCCCGTGGCTGCTGTGCGGACACGGGTGCAGCATGGCTCTAACAAGAGA
AGAGATCACAGAAACGTGAGGATCAAGAAGCCGGACTACAAGGTCGCCTACGTGCAGCTG
GCCCATGGACAGACCTTCACGTTCCCAGATCTGTTTCCCGAGAAAGACGAGAGCCCTGAA
GGCAGCGCTGCCGACGACCTCTACAGCATGCTCGAGGAGGAGAGGCAGCAGAGGCAGAGC
AGCGACCCGCGGCGGGGGCGGCGTCCCCAGCTGGTTCGGGCTGTGACGGNGTGGCCAGCA
GGGACGCGCCCCAGGTGGGCAGCTGTGGCAGAGCAGCGACCCGCGGCGGGGCGGCATCCC
AGCTGGGTTCGGGCCGTGACGGGGGCGGCCAGCAGGGACGCGCCCCAGGTGGGCAGCTGT
GGCAAAGCAGTCCCGACACCTANATANNAGTCTTGCTGCAGGAAAAAAAAAAAAAAAAAC
TCGACTCTAGNATTGCGGCCCGGGTCATAAGCTGGTTCCTGAACAGATCCCGGGGTGCCA
TCCCTGTGACCCCTCCCCAGTGCCTCTCCTGCCCTTGGAAGTTGCCACTCCAGTGCCCAC
CANCTTTGTCTATAAAATTAAGTTGCTNATTTTGCTGAACTAGGGTCCTTTATAAATATG
GGNGNGGGGNGGGGGGGGTNNNNNNNNANGGGNNANNNGNNNAAACANNNNNAGCCGCCG
CCGCCCCTTGGGGACCAACCTGGTGGGGACGCACAACA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_021134 unedited
AGAGTCGAGTTTTTTTTTTTTTTTTTTCCTGCAGCAAGACTTTTATTTAGGTGTCGGGAC
TGCTCTGCCACAGCTGCCCACCTGGGGCGCGTCCCTGCTGGCCGCCCCGTCACGGCCCGA
ACCAGCTGGGGATGCCGCCCCGCCGCGGGTCGCTGCTCTGCCACAGCTGCCCACCTGGGG
CGCGTCCCTGCTGGCCACCCCGTCACAGCCCGAACCAGCTGGGGACGCCGCCCCGCCGCG
GGTCGCTGCTCTGCCTCTGCTGCCTCTCCTCCTCGAGCATGCTGTAAAGGTCGTCGGCAG
CGCTGCCTTCAGGGCTCTCGTCTTTCTCGGGAAACAGATCTGGGAACGTGAAGGTCTGTC
CATGGGCCAGCTGCACGTAGGCGACCTTGTAGTCCGGCTTCTTGATCCTCACGTTTCTGT
GATCTCTTCTCTTGTTAGAGCCATGCTGCACCCGTGTCCGCACAGCAGCCACGGGCACGT
TATAGATGCCCTCGAGGTAATTCCTGAGGTCCACCCTTGTCATTTCCATGGGGATCCGGA
ACTGCACGGTGTCCTCGGGCTGGGCCACACCGGGCCGCACCAGCTGAATGAAAAAGTTGT
TCCGGACACCCCGAATTGGGGCCACCCACCCGTACAGGGGGTACACCACATTCCGCGCCA
TGGTGGACACGCCCGCTTCCGGGGCCTCGTGCCGAATTCCGGCCGCCCTAAATGATTCGA
TTCCAAATTCTGACGGTTACTAAACAAACCTGCTTATTAAACCTCCCCCGACACCCCTAC
GCCCTTTTGGCTAACGGGGGGGGTTATACCACTTTTGAAAACCCCGTGATTCGGGGGCCA
AACACCCTCCCTCGCGCCCATGGGGGGAAACTTGTAACCCCGGGACCACACCCTTTCACG
CCCTGTGGGCTTGCCACCGCACCCATGGAAAGCCCACCCACCCTAAGTTCCGCCAAGAAA
ATCCCCAGGGTTTCACGGCAAACCCAGCGCCTTTTCCCTTGCTCAAAGGGCGCCTGCCTC
ACCT
Restriction Sites NotI-NotI |
ACCN NM_021134
Insert Size 730 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021134.2, NP_066957.2
RefSeq Size 701 bp
RefSeq ORF 462 bp
Locus ID 6150
UniProt ID Q16540
Cytogenetics 11p15.5
Protein Families Druggable Genome
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11. provided by RefSeq, Jul 2008
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Documents
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"NM_021134" in other vectors (6)
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RC202918 MRPL23 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L23 (MRPL23), nuclear gene encoding mitochondrial protein 10 ug
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RC202918L1 Lenti ORF clone of Human mitochondrial ribosomal protein L23 (MRPL23), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
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RC202918L2 Lenti ORF clone of Human mitochondrial ribosomal protein L23 (MRPL23), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
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RC202918L3 Lenti ORF clone of Human mitochondrial ribosomal protein L23 (MRPL23), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
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RC202918L4 Lenti ORF clone of Human mitochondrial ribosomal protein L23 (MRPL23), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
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RG202918 MRPL23 (tGFP-tagged) - Human mitochondrial ribosomal protein L23 (MRPL23), nuclear gene encoding mitochondrial protein 10 ug
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