GOLPH3 (NM_022130) Human Untagged Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | GOLPH3 |
| Synonyms | GOPP1; GPP34; MIDAS; Vps74 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | ECoRI-NOT | |
| ACCN | NM_022130 |
| Insert Size | 3200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_022130.3, NP_071413.1 |
| RefSeq Size | 2676 bp |
| RefSeq ORF | 897 bp |
| Locus ID | 64083 |
| UniProt ID | Q9H4A6 |
| Cytogenetics | 5p13.3 |
| Summary | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_022130" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207477 | GOLPH3 (Myc-DDK-tagged)-Human golgi phosphoprotein 3 (coat-protein) (GOLPH3) | 10 ug |
$450.00
|
|
| RC207477L1 | Lenti ORF clone of Human golgi phosphoprotein 3 (coat-protein) (GOLPH3), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC207477L2 | Lenti ORF clone of Human golgi phosphoprotein 3 (coat-protein) (GOLPH3), mGFP tagged | 10 ug |
$750.00
|
|
| RC207477L3 | Lenti ORF clone of Human golgi phosphoprotein 3 (coat-protein) (GOLPH3), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC207477L4 | Lenti ORF clone of Human golgi phosphoprotein 3 (coat-protein) (GOLPH3), mGFP tagged | 10 ug |
$750.00
|
|
| RG207477 | GOLPH3 (tGFP-tagged) - Human golgi phosphoprotein 3 (coat-protein) (GOLPH3) | 10 ug |
$650.00
|
|
| SC323962 | GOLPH3 (untagged)-Human golgi phosphoprotein 3 (coat-protein) (GOLPH3) | 10 ug |
$450.00
|
|
Citations
Punicalagin inhibits the viability, migration, invasion, and EMT by regulating GOLPH3 in breast cancer cells
Pan, Duan, Ma et al
J. Recept. Signal Transduct. Res. (2020) 40 (2), 173-180 DOI: 10.1080/10799893.2020.1719152
Pan, Duan, Ma et al
J. Recept. Signal Transduct. Res. (2020) 40 (2), 173-180 DOI: 10.1080/10799893.2020.1719152
Cited by 42
Retrograde Transport of Golgi Enzymes by GOLPH3 Across Maturing Cisternae Regulates Glycan Assembly on Sphingolipids and Cell Growth
Rizzo, Russo, Kurokawa et al
bioRxiv (2019)
Rizzo, Russo, Kurokawa et al
bioRxiv (2019)
Cited by 5
Snippet: The oligos were ordered for creating siRNA-1# resistant GOLPH3 by changing the nucleotide sequence, but not the codon sequence. … The GOLPH3 cDNA was cloned in pCMV6-XL5 vector (Origene). Pfu Turbo DNA polymerase was used to insert and amplify the 3-point-mutagenized GOLPH3 sequence, amplifying for 18 cycles at 56 C and extending the mutagenized DNA 16 min at 68 C at each cycle. …
Showing 1-2 of 2 papers.
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