XYLT2 (NM_022167) Human Untagged Clone

SKU SC112785

XYLT2 (untagged)-Human xylosyltransferase II (XYLT2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: XYLT2
• Synonyms: PXYLT2; SOS; XT-II; XT2; xylT-II
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112785 sequence for NM_022167 edited (data generated by NextGen Sequencing); ATGGTGGCGAGCGCGCGAGTGCAGAAGCTGGTGCGGCGCTACAAGCTGGCGATTGCCACG; GCGCTGGCCATCCTGCTGCTGCAGGGCCTGGTAGTGTGGAGCTTCAGCGGCCTGGAGGAG; GACGAGGCGGGCGAGAAAGGAAGGCAGAGGAAGCCACGGCCACTGGACCCTGGCGAAGGT; TCCAAGGACACAGACAGTTCAGCAGGGCGACGGGGCAGCACAGGCAGAAGGCATGGGCGC; TGGCGGGGCCGTGCTGAGAGCCCAGGAGTGCCCGTGGCCAAGGTGGTACGGGCAGTAACC; AGCCGGCAGAGAGCCAGCCGGCGGGTCCCACCTGCCCCACCTCCGGAAGCCCCAGGCCGC; CAGAACCTGAGTGGGGCAGCAGCTGGGGAGGCGCTGGTAGGGGCAGCTGGCTTCCCACCA; CACGGAGATACAGGGAGCGTGGAGGGCGCCCCCCAGCCCACGGACAATGGCTTCACCCCC; AAGTGCGAGATCGTGGGCAAGGACGCACTGTCTGCACTGGCCCGGGCCAGCACCAAGCAG; TGCCAGCAGGAGATCGCCAATGTGGTGTGCCTGCACCAGGCTGGGAGCCTCATGCCCAAG; GCTGTGCCCCGGCACTGTCAGCTGACTGGGAAGATGAGCCCCGGCATCCAGTGGGATGAG; AGCCAAGCCCAGCAGCCCATGGATGGCCCCCCGGTGCGAATCGCCTACATGCTGGTGGTT; CACGGCCGCGCCATCCGCCAGCTGAAGCGTCTCCTCAAGGCCGTTTATCACGAGCAGCAC; TTCTTTTACATCCATGTGGACAAGCGTTCCGACTACCTGCACCGGGAGGTGGTGGAGCTG; GCCCAGGGCTATGATAACGTGCGGGTGACGCCCTGGCGCATGGTTACCATCTGGGGCGGG; GCCAGCCTCCTGACGATGTACCTGCGGAGCATGCGGGACCTGCTAGAGGTGCCTGGCTGG; GCCTGGGACTTCTTCATCAACCTCAGTGCCACTGACTATCCAACCAGGACCAATGAGGAG; CTGGTGGCATTCCTATCCAAGAACCGGGACAAGAATTTCCTCAAGTCACATGGCCGGGAC; AACTCCAGGTTCATCAAGAAACAGGGCCTGGACCGGCTCTTCCATGAGTGCGACTCACAC; ATGTGGCGCCTGGGCGAGCGGCAGATCCCAGCAGGCATTGTGGTGGATGGCGGTTCTGAC; TGGTTCGTGCTGACACGCAGCTTTGTGGAGTATGTGGTGTACACAGATGACCCGCTTGTG; GCCCAGCTGCGCCAGTTCTACACATACACACTGCTCCCAGCCGAGTCCTTCTTCCACACG; GTGCTGGAGAACAGCCTGGCCTGTGAGACCCTCGTGGACAACAACCTGCGGGTCACCAAC; TGGAACCGCAAGCTGGGCTGCAAGTGCCAGTACAAGCACATTGTGGACTGGTGTGGCTGC; TCCCCCAACGACTTCAAGCCACAGGACTTCCTCCGGCTGCAGCAAGTCTCCAGACCCACC; TTCTTCGCCCGGAAGTTCGAGTCGACTGTGAACCAGGAGGTGCTGGAAATCCTGGACTTC; CACCTGTACGGCAGCTACCCCCCCGGCACGCCAGCCCTCAAGGCCTACTGGGAGAACACC; TACGACGCGGCTGATGGCCCCAGTGGGCTCAGTGATGTCATGCTCACTGCTTACACAGCC; TTCGCCCGCCTCAGCCTGCACCATGCCGCCACTGCTGCACCCCCAATGGGCACCCCACTC; TGCAGGTTTGAGCCCAGGGGCTTGCCGTCCAGCGTGCACCTGTATTTCTATGACGACCAT; TTCCAGGGCTACCTGGTGACGCAGGCGGTGCAGCCCTCAGCCCAGGGGCCGGCAGAGACG; CTTGAGATGTGGCTGATGCCCCAAGGGTCGCTGAAGCTGTTGGGGCGCAGTGACCAGGCC; AGCCGGCTCCAGAGTCTGGAGGTTGGCACTGATTGGGACCCCAAAGAGCGTCTTTTCCGG; AACTTTGGGGGGTTACTGGGGCCGCTGGACGAGCCTGTGGCCGTGCAGCGCTGGGCCCGG; GGCCCCAACCTCACAGCCACAGTGGTCTGGATCGACCCAACCTATGTGGTGGCCACATCT; TATGACATCACAGTAGATACGGAGACTGAGGTCACGCAATACAAGCCCCCACTGAGCCGG; CCCCTGCGGCCAGGGCCCTGGACTGTTCGACTCCTTCAGTTCTGGGAACCGCTGGGTGAG; ACCCGCTTCCTTGTGCTGCCCTTGACCTTCAACCGCAAACTACCTCTCAGGAAAGATGAT; GCCAGCTGGCTGCACGCAGGGCCACCCCACAACGAGTACATGGAGCAGAGTTTCCAGGGC; CTGAGTAGCATCCTGAACCTGCCTCAGCCGGAGCTCGCGGAGGAGGCTGCCCAGCGGCAC; ACACAGCTCACAGGCCCTGCGCTCGAGGCCTGGACAGACAGGGAACTGAGCAGCTTCTGG; TCCGTGGCTGGACTGTGTGCCATAGGCCCCTCTCCCTGCCCCTCCCTGGAGCCCTGCAGA; CTGACCAGCTGGAGCTCTCTGTCCCCCGACCCCAAATCAGAGCTGGGGCCTGTCAAAGCA; GACGGGCGACTCAGGTAG; ; Clone variation with respect to NM_022167.2; 177 g=>a;342 c=>t;914 g=>c;1569 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022167 unedited; TATAACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA; GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG; CGAATTCGGCACGAGGGTTGCAGGGCTGGGCGCGCGCCCCGCGTCCCGGGCAGGAAGATG; GTGGCGAGCGCGCGAGTGCAGAAGCTGGTGCGGCGCTACAAGCTGGCGATTGCCACGGCG; CTGGCCATCCTGCTGCTGCAGGGCCTGGTAGTGTGGAGCTTCAGCGGCCTGGAGGAGGAC; GAGGCGGGCGAGAAAGGAAGGCAGAGGAAGCCACGGCCACTGGACCCTGGCGAAGGTTCC; AAGGACACAGACAGTTCAGCAGGGCGACGGGGCAGCACAGGCAGAAGGCATGGGCGCTGG; CGGGGCCGTGCTGAGAGCCCAGGAGTGCCCGTGGCCAAGGTGGTACGGGCAGTAACCAGC; CGGCAGAGAGCCAGCCGGCGGGTCCCACCTGCCCCACCTCCGGAAGCCCCAGGCCGCCAG; AACCTGAGTGGGGCAGCAGCTGGGGAGGCGCTGGTAGGGGCAGCTGGCTTCCCACCACAC; GGAGATACAGGGAGCGTGGAGGGCGCCCCCCAGCCCACGGACAATGGCTTCACCCCCAAG; TGCGAGATCGTGGGCAAGGACGCACTGTCTGCACTGGCCCGGGCCAGCACCAAGCAGTGC; CAGCAGGAGATCGCCAATGTGGTGTGCCTGCACCAGGCTGGGAGCCTCATGCCCAAGGCT; GTGCCCCGGCACTGTCAGCTGACTGGGAAGATGAGCCCCGGCATCCAGTGGGATGAGAGC; CAAGCCCAGCAGCCCATGGATGGCCCCNCGGTGCGAATCGNCTCATGCTGGTGGTTCACG; GCCGCGCCATCCGCCAGCTGGAGCGTCTNCTCAAGGCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022167 unedited; CGGGGAAATACTATGTACGCGGCCGCTTTCTANGATCGATTTTTTTTTTTTTTTTTTAGA; GGAGAAGAGATATATTTCTTCTGTTGCATATTTCAGACTTCACGCTGAGCTTCAGGCACA; ATTACTCCAGGTTCTTCTGAGAGTCTTGACAGAGTCTTCTTCCCACGCTGCTCATGTGAC; CTCACTTCCAGTGCACACCTGGAAACTCTGCATGGGTCTCATTGGGCCCTGACCCTGCCT; TTCCCATCCAGGGGCAGGGTATGCTGGAAAGCCCTGGAGCCGCCCCACCCCACTTCTAGG; GTCCCCTGGCTACAAAAGAACTGTCCTGCTAGAGCCCAGCTCTGCAGCGCCCCCGATATC; ACCTGTTCTTCGGGCCTTAGAGCCTGGCAGGGCACTTGCAGCCCTGTGGAGCCTCAGCCA; CGAGCACCACAGGCCCAGAATGGGGCATGAGGCCCTGGAGAGTGAGGCAAGCCCAGCCTC; GTGCAGAACAATAGCAGGTGGGCTCAACAAGCCCCTGCTCCCCACAGACATGGCCCACTT; CCTGGGGCTGCTCTGAGCCCTATTGCACATCTTCTGGTCTAGTTTCTCTCCCAACCACCC; ATTTTCCTTTTTAAATTTAAAACAAAAAAAGAAAAAGAAAAAAAAGAAATTCCCACTATA; CCGGAAGGTGAAGAAGAACTGGGGGTGGAATGCTGCTGTGCCCCTTTTGGGTTTCCGCCA; GCCCTCTGGATCCCCACAAAAAACTCAGCAGTAGTTTATAATGGGGCCACCTTTCCTGGC; CCCTGTGTGGGGTTCTTGAAGGGTCAAAAGGGGGGGCAACCCTGGGCCCCTGGTTTCTTG; CCCGGTGGGAGAGGAGAACCCCTCCCCTGGGTTGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_022167
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022167.1, NP_071450.1
• RefSeq Size: 3568 bp
• RefSeq ORF: 2598 bp
• Locus ID: 64132
• UniProt ID: Q9H1B5
• Cytogenetics: 17q21.33
• Domains: Branch
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
• Summary: The protein encoded by this gene is an isoform of xylosyltransferase, which belongs to a family of glycosyltransferases. This enzyme transfers xylose from UDP-xylose to specific serine residues of the core protein and initiates the biosynthesis of glycosaminoglycan chains in proteoglycans including chondroitin sulfate, heparan sulfate, heparin and dermatan sulfate. The enzyme activity, which is increased in scleroderma patients, is a diagnostic marker for the determination of sclerotic activity in systemic sclerosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]; Transcript Variant: This variant (1) represents the protein-coding transcript.