SMPD4 (NM_017951) Human Untagged Clone

SKU SC112589

SMPD4 (untagged)-Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SMPD4
• Synonyms: NEDMABA; NEDMEBA; NET13; NSMASE-3; NSMASE3; SKNY
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017951, the custom clone sequence may differ by one or more nucleotides; ; ; ATGACGACTTTCGGCGCCGTGGCGGAATGGCGGCTTCCATCTCTGAGGCGAGCGACGCTATGGATCCCAC; AGTGGTTTGCTAAGAAGGCCATTTTCAACTCTCCACTGGAGGCTGCTATGGCGTTCCCTCACCTGCAGCA; GCCCAGCTTTCTACTGGCTAGCCTGAAAGCTGACTCTATAAATAAGCCCTTTGCACAGCAGTGCCAAGAC; TTGGTTAAAGTCATTGAGGACTTTCCAGCAAAGGAGCTGCACACCATCTTCCCATGGCTGGTAGAAAGCA; TTTTTGGCAGCCTAGATGGTGTCCTCGTTGGCTGGAACCTCCGCTGCTTACAGGGGCGCGTGAATCCTGT; GGAGTACAGCATCGTGATGGAATTTCTCGACCCTGGTGGCCCAATGATGAAGTTGGTTTATAAGCTTCAA; GCTGAAGACTATAAGTTCGACTTTCCTGTCTCCTACTTGCCTGGTCCTGTGAAGGCGTCCATCCAGGAGT; GCATCCTCCCTGACAGTCCTCTGTACCACAACAAGGTCCAGTTCACCCCTACTGGGGGCCTTGGTCTGAA; CTTGGCCCTGAATCCGTTCGAGTATTACATATTCTTCTTTGCCTTGAGCCTCATCACTCAGAAGCCACTT; CCTGTGTCCCTCCACGTCCGTACTTCAGACTGTGCCTATTTCATCCTGGTGGACAGGTACCTGTCATGGT; TCCTGCCCACCGAAGGCAGTGTGCCCCCACCACTCTCCTCCAGCCCAGGGGGGACCAGCCCCTCACCACC; TCCCAGGACACCAGCCATACCCTTTGCTTCCTATGGCCTCCACCACACTAGCCTCCTAAAGCGACACATC; TCTCATCAGACGTCTGTGAATGCAGACCCCGCCTCCCACGAGATCTGGAGGTCAGAAACTCTGCTCCAGG; TTTTTGTTGAAATGTGGCTTCATCACTATTCCTTGGAGATGTATCAAAAAATGCAGTCCCCTCATGCCAA; GCTGGAGGTTCTGCACTACCGACTCAGTGTCTCCAGCGCCCTCTACAGCCCCGCCCAACCCAGCCTCCAG; GCCCTCCACGCCTACCAAGAGTCGTTCACGCCTACTGAGGAGCATGTGTTGGTGGTGCGCCTGCTGCTGA; AGCACCTGCACGCCTTTGCCAACAGCCTGAAGCCAGAGCAGGCCTCACCCTCCGCCCACTCCCACGCCAC; CAGCCCCCTGGAGGAGTTCAAACGGGCTGCTGTCCCGAGGTTCGTCCAGCAGAAACTCTACCTCTTCTTG; CAGCATTGCTTTGGCCACTGGCCCCTGGACGCATCGTTCAGAGCTGTCCTGGAGATGTGGCTGAGCTACC; TGCAGCCGTGGCGGTACGCGCCTGACAAGCAGGCTCCGGGCAGCGACTCCCAGCCCCGGTGTGTGTCGGA; GAAATGGGCACCCTTTGTCCAGGAGAACCTGCTGATGTACACCAAGTTGTTTGTGGGCTTTCTGAACCGC; GCGCTCCGCACAGACCTGGTCAGCCCCAAGCACGCGCTCATGGTGTTCCGAGTGGCCAAAGTCTTTGCCC; AGCCCAACCTGGCTGAGATGATTCAGAAAGGTGAGCAGCTATTCCTGGAGCCAGAGCTGGTCATCCCCCA; CCGCCAGCACCGACTCTTCACGGCCCCCACATTCACTGGGAGCTTCCTGTCACCCTGGCCACCAGCGGTC; ACTGATGCCTCCTTCAAGGTGAAGAGCCACGTCTACAGCCTGGAGGGCCAGGACTGCAAGTACACCCCGA; TGTTTGGGCCCGAGGCCCGCACCCTGGTCCTGCGCCTCGCTCAGCTCATCACACAGGCCAAACACACAGC; CAAGTCCATCTCCGACCAGTGTGCGGAGAGCCCGGCTGGCCACTCCTTCCTCTCATGGCTGGGCTTTAGC; TCCATGGACACCAATGGCTCCTACACAGCCAACGACCTGGACGAGATGGGGCAAGACAGTGTCCGGAAGA; CAGATGAATACCTGGAGAAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCGCAGCTCAG; GCAGTTCACACTCGCCTTGGGCACCACCCAGGATGAGAATGGAAAAAAGCAACTCCCCGACTGCATCGTG; GGTGAGGACGGACTCATCCTTACGCCCCTGGGGCGGTACCAGATCATCAATGGGCTGCGAAGGTTTGAAA; TTGAGTACCAGGGGGACCCGGAGCTGCAGCCCATCCGGAGCTATGAGATCGCCAGCTTGGTCCGCACACT; CTTTAGGCTGTCGTCTGCCATCAACCACAGATTTGCAGGACAGATGGCGGCTCTGTGTTCCCGGGATGAC; TTCCTCGGCAGCTTCTGTCGCTACCACCTCACAGAACCTGGGCTGGCCAGCAGGCACCTGCTGAGCCCTG; TGGGGCGGAGGCAGGTGGCCGGCCACACCCGCGGCCCCAGGCTCAGCCTGCGCTTCCTGGGCAGTTACCG; GACGCTGGTCTCGCTGCTGCTGGCCTTCTTCGTGGCCTCTCTGTTCTGCGTCGGGCCCCTCCCATGCACG; CTGCTGCTCACCCTGGGCTATGTCCTCTACGCCTCTGCCATGACACTGCTGACCGAGCGGGGGAAGCTGC; ACCAGCCCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017951 unedited; NNCCGTTCGAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGGAAT; GGCGGCTTCCATCTCTGAGGCGAGCGACGCTATGGATCCCACAGTGGTTTGCTAAGAAGG; CCATTTTCAACTCTCCACTGGAGGCTGCTATGGCGTTCCCTCACCTGCAGCAGCCCAGCT; TTCTACTGGCTAGCCTGAAAGCTGACTCTATAAATAAGCCCTTTGCACAGCAGTGCCAAG; ACTTGGTTAAAGTCATTGAGGACTTTCCAGCAAAGGAGCTGCACACCATCTTCCCATGGC; TGGTAGAAAGCATTTTTGGCAGCCTAGATGGTGTCCTCGTTGGCTGGAACCTCCGCTGCT; TACAGGGGCGCGTGAATCCTGTGGAGTACAGCATCGTGATGGAATTTCTCGACCCTGGTG; GCCCAATGATGAAGTTGGTTTATAAGCTTCAAGCTGAAGACTATAAGTTCGACTTTCCTG; TCTCCTACTTGCCTGGTCCTGTGAAGGCGTCCATCCAGGAGTGCATCCTCCCTGACAGTC; CTCTGTACCACAACAAGGTCCAGTTCACCCCTACTGGGGGCCTTGGTCTGAACTTGGCCC; TGAATCCGTTCGAGTATTACATATTCTTCTTTGCCTTGAGCCTCATCACTCAGAAGCCAC; TTCCTGTGTCCCTCCACGTCCGTACTTCAGACTGTGCCTATTTCATCCTGGTGGACAGGT; ACCTGTCATGGTTCCTGCCCACCGAAAGCAGTGTGCCCCCACCACTCTCCTCCAGCCCAG; GGGGACCAGCCCCTCACCACCTCCCAGACACCAGCCATACCCTTTGCTTCCTATGGCCTN; CACCACACTAGCCCTCCTAAGCGACACATCTCTCATCAGAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017951 unedited; NGGCTAGGGGCATGAAACTTNATTTCCATGGTCGTTACAGCCTTCATTTTTGNGTCACAG; NGCCCTAAAATGCCATTATGCTCAGTTTACATCAAAATACAAAGCCCAACATTTACAATT; TCAAAAACATTAAAGCAAACCCCCAAAACCCCNACACCGAAAACAAAGGCTTGGTTTGGA; AATCACCACTGAGATGCTGTTCTCCCTCGCATGTCGCCTGTGGGGTGAATGAAGGTGAAG; ACCCCGTGCTGGTTTCTGTCAGAGAATCTGTAGTTGTATATTTTGAATACTTAAGTACCA; CTGAACGGTTGCCATGTCTTTGAGCACTTGATGTTACTGAAGTGACAATGCTTCTGACCA; TCTTTGGCTGCAGAGCAGAAACTGGCAGAATCCGACCCCATCGACCCAGCAGTGTCCCCA; ACGCCTGAGGACCAGCACCCATCTGTGACAAGGCACAGGGGTCCGCGATGTTGGCCACTG; CTTTGGGTGATGTGCTCAATGGCTTGGGGTCTGCGGTGAAGCCCCTGGGCTCTGGGAGGG; TCTTAGGCTCCTGAAACATGGAATTCTACGGCCCAGTCTTGGAACAGCTGGGAGAACATG; AAGAACGCCCCTCACAGTGAAGAATCAGGACAGCCACTCTCTGGTTTTCTCACAACTCTA; GAAACTTTGATTCTTTCCTGGAGTGGAGACTTAAACCCTGTCCCTGTCCCACCCAGAGAG; AGGAGCGCCCCAACCCACTCTGCCACCCTTAAGGCCGCCCGCGGGGGAAGCCAGGCCCAG; GTGGCTGTTTGCAGGCTGGCTCTCCAGCTTGGTGCAACAAAATACCCAAAGGGCCTGGGG; AAAAAAGGCCCCNAAGCTTGTTTGCCCCGAATCCTTGCCGAGGGAGGGAAAAGCCGCCCT; TAATCTTTGCCTTGCCAAAAAGTACTTTTGGACCTGTGAATACCCCTTCTTGGAGCCACC; CA;
• Restriction Sites: NotI-NotI
• ACCN: NM_017951
• Insert Size: 4080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017951.1, NP_060421.1
• RefSeq Size: 3920 bp
• RefSeq ORF: 1818 bp
• Locus ID: 55627
• UniProt ID: Q9NXE4
• Cytogenetics: 2q21.1
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Summary: The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. [provided by RefSeq, Mar 2017]; Transcript Variant: This variant (2) encodes the longest isoform (2). CCDS Note: This CCDS representation uses the 5'-most in-frame start codon. While this start codon is well-conserved, it should be noted that other than human and chimp, all other species have indels that follow this start codon. Other primates and rodents have frameshifting indels, and thus cannot use this start codon. However, some mammalian species, including cow, pig and dog, contain additional compensating indels that bring them back in frame; these species could therefore use the start codon represented in this CCDS. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 39 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo.