MTMR14 (NM_022485) Human Untagged Clone

SKU SC112499

MTMR14 (untagged)-Human myotubularin related protein 14 (MTMR14), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: MTMR14
• Synonyms: C3orf29
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022485, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCGGCGCTCGGGCCGCCGCCGCCGCTGCCTCGGCGGGGTCCTCGGCCTCTTCAGGCAACCAGCCGC; CTCAGGAGCTGGGGCTTGGGGAGCTGCTGGAGGAGTTCTCCCGGACTCAGTACCGGGCCAAGGATGGCAG; CGGGACCGGCGGCTCTAAGGTTGAGCGCATTGAGAAGAGATGTCTGGAGCTGTTTGGCCGAGACTACTGT; TTCAGCGTGATTCCAAACACGAATGGGGATATCTGTGGCCACTATCCCCGGCACATCGTGTTCCTGGAGT; ATGAGAGTTCTGAGAAGGAGAAAGACACGTTTGAGAGTACCGTACAGGTGAGCAAGTTGCAAGACCTCAT; CCACCGCAGCAAGATGGCCCGGTGCAGAGGACGGTTTGTCTGCCCAGTAATCCTGTTCAAGGGCAAGCAC; ATTTGCAGGTCGGCCACACTGGCTGGATGGGGAGAGCTGTATGGACGCTCAGGCTACAACTATTTTTTCT; CAGGGGGTGCAGATGATGCCTGGGCAGATGTGGAGGACGTCACGGAGGAGGACTGTGCTCTTCGAAGTGG; TGACACGCATCTTTTTGATAAGGTCAGAGGCTATGACATCAAGCTGCTTCGATACCTGTCAGTCAAATAC; ATCTGTGACCTGATGGTGGAGAACAAGAAGGTGAAGTTTGGCATGAATGTAACCTCCTCTGAGAAGGTGG; ACAAAGCCCAGCGCTATGCCGACTTCACTCTCCTCTCCATCCCGTATCCAGGCTGTGAATTTTTCAAGGA; ATATAAAGATCGGGATTACATGGCAGAAGGGCTCATATTTAACTGGAAGCAGGACTACGTTGATGCCCCA; TTGAGCATCCCCGACTTCCTGACTCACTCTCTGAACATTGACTGGAGCCAGTATCAGTGTTGGGATCTGG; TGCAACAAACACAAAACTACCTGAAGCTGCTGCTTTCCTTAGTTAACAGTGATGATGACAGCGGGCTGCT; GGTACACTGTATCTCAGGCTGGGATCGGACCCCCCTCTTCATCTCCCTCCTGCGCCTTTCCTTGTGGGCT; GATGGGCTCATCCACACGTCCCTGAAGCCCACTGAGATCCTCTACCTCACTGTGGCCTATGACTGGTTCC; TCTTCGGGCACATGTTGGTAGATCGGCTCAGCAAAGGGGAGGAGATTTTCTTCTTCTGCTTCAATTTTTT; GAAGCATATTACCTCCGAGGAGTTCTCTGCTCTGAAGACCCAGAGGAGGAAGAGTTTGCCAGCCCGGGAT; GGAGGCTTCACCCTGGAAGACATCTGCATGCTGAGACGAAAGGACCGTGGCAGCACCACCAGCCTTGGCA; GCGACTTCTCCCTGGTCATGGAGAGTTCCCCAGGAGCCACTGGGAGCTTCACCTATGAGGCCGTGGAGCT; GGTCCCAGCAGGAGCGCCAACTCAGGCAGCTTGGCTTGCAGCCCTGAGTGATCGAGAGACTCGGCTGCAG; GAGGTGCGCTCAGCCTTCTTGGCTGCGTACAGCAGCACAGTGGGGCTTCGGGCAGTAGCCCCCAGTCCTT; CCGGTGCCATCGGGGGCCTGCTGGAGCAATTTGCCCGTGGTGTTGGACTCCGGAGCATCAGCAGCAATGC; CTTGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022485 unedited; NGGTTGTCAAATTTGTAAACCATCATATAGGCGGCCGCGAAATTCGCACGAGGCGGTTCC; GGNAGTTCTAGTGTCGGAGTTGGGTGCAGGCAGGTGCCATGGGCCCGCTTGAGGCACACT; GAGGGGACGCGGGGCTGGGCCATGGCCGGCGCTCGGGCCGCCGCCGCCGCTGCCTCGGCG; GGGTCCTCGGCCTCTTCAGGCAACCAGCCGCCTCAGGAGCTGGGGCTTGGGGAGCTGCTG; GAGGAGTTCTCCCGGACTCAGTACCGGGCCAAGGATGGCAGCGGGACCGGCGGCTCTAAG; GTTGAGCGCATTGAGAAGAGATGTCTGGAGCTGTTTGGCCGAGACTACTGTTTCAGCGTG; ATTCCAAACACGAATGGGGATATCTGTGGCCACTATCCCCGGCACATCGTGTTCCTGGAG; TATGAGAGTTCTGAGAAGGAGAAAGACACGTTTGAGAGTACCGTACAGGTGAGCAAGTTG; CAAGACCTCATCCACCGCAGCAAGATGGCCCGGTGCAGAGGACGGTTTGTCTGCCCAGTA; ATCCTGTTCAAGGGCAAGCACATTTGCAGGTCGGCCACACTGGCTGGATGGGGAGAGCTG; TATGGACGCTCAGGCTACAACTATTTTTTCTCAGGGGGTGCAGATGATGCCTGNGCAGAT; GTGGAGGACGTCACGGAGGAGGACTGTGCTCTTCGAAGTGGTGACACGCATCTTTTTGAT; AAGGTCAGAGGCTATGACATCAAGCTGCTTCGATACCTGTCAGTCAAATACATCTGTGAC; CTGATGGTGGAGAACAAGAANNGTGAAGTTGGCATGAATGTAACCTNCTCTGAGAAAGTG; GNACAAGCCAGCGCTATGCCGACTTACTCTCTCTNCATCCGNATCCNCCGATAAGATAGT; AGCTGGCGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022485 unedited; CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGCTATTCAAAAAACTTT; GATTTTATTTTGTGATCTGTGTCAAACTGCCTCATTCTCAGTGCAGGTGATCCCTCCTCC; CCAGAAACAAGAACTGACAGGTAGGGTCTTTGTGAAGTCATGGCTTCCACAAGGCCCCAG; GGAAACCCCACACAGTTCTGAGAGTTGATGGGAATCCCAGCAAAGATGGGGTGGGTGGGA; GACAGTGACAAGAAGGGGTGGTGGTCACCAGTCAAGAGGCTGGGGGTTGGAAAACCCTGT; CTTTGGCCTTGCTCAGCTTTGGGGCTGCCATGTTCATGATGGAGATGGGGGACTGAAATT; TCCCCTGACCTGGAAGTACCCCTTCGGCCAGGCATGGCTTTGATTCTCTGCTAAGGGCTC; AGCTGAGAGAGGAGCAGGAAAATGTCATGGGCTGGCTTCTTCACAAGGCATTGCTGCTGA; TGCTCCGGAGTCCAACACCACGGGCAAATTGCTCCAGCAGGCCCCCGATGGCACCGGAAG; GACTGGGGGCTACTGCCCGAAGCCCCACTGTGCTGCTGTACGCAGCCAAGAAGGCTGAGC; GCACCTCCTGCAGCCGAGTCTCTCGATCACTCAGGGCTGNCAGCAGACAACTGTTATGGA; GCTCATCCGGGAAGCTGAAAGGGAGAGAAGAGTCTGTGTGCAGGACAGCCCGCTCCTGAA; TACTGCCACAGCCCNGTACCATCTGCCAGCTGCCATAGTCTGTTGAGAGAGAAGATCCNG; GCAGGGGATGGTCCACTGATCTGGNTTGGGGACCTCCACGGGACTGCTACCATCCTGAAA; AAGTATTAAATGGNTTGAGGAAAAGAGCTGNANCACTTGCCTTNCGCAGCCTCTGCTGGG; AAGCCAGCCGTCTCTGAGGTTGTGGCCGTTTCAGAGAACTTTGTGAAAGGATGATGCTTT; TCTCCAGCTGCTGAATGCGCTCTGCTGGACAATTCCGNCTTTAGGAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_022485
• Insert Size: 2570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022485.2, NP_071930.1
• RefSeq Size: 2200 bp
• RefSeq ORF: 1254 bp
• Locus ID: 64419
• UniProt ID: Q8NCE2
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome, Phosphatase, Secreted Protein
• Summary: This gene encodes a myotubularin-related protein. The encoded protein is a phosphoinositide phosphatase that specifically dephosphorylates phosphatidylinositol 3,5-biphosphate and phosphatidylinositol 3-phosphate. Mutations in this gene are correlated with autosomal dominant centronuclear myopathy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 18.[provided by RefSeq, Apr 2010]; Transcript Variant: This variant (3) lacks two alternate in-frame segments in the 3' coding region, compared to variant 2. The resulting protein (isoform 3) has a shorter C-terminus when it is compared to isoform 2.