SIL1 (NM_022464) Human Untagged Clone

SKU SC112483

SIL1 (untagged)-Human SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae) (SIL1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SIL1
• Synonyms: BAP; MSS; ULG5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112483 sequence for NM_022464 edited (data generated by NextGen Sequencing); ATGGCTCCCCAGAGCCTGCCTTCATCTAGGATGGCTCCTCTGGGCATGCTGCTTGGGCTG; CTGATGGCCGCCTGCTTCACCTTCTGCCTCAGTCATCAGAACCTGAAGGAGTTTGCCCTG; ACCAACCCAGAGAAGAGCAGCACCAAAGAAACGGAGAGAAAAGAAACCAAAGCCGAGGAG; GAGCTGGATGCTGAAGTCCTGGAGGTGTTCCACCCGACGCATGAGTGGCAGGCCCTTCAG; CCAGGGCAGGCTGTCCCTGCAGGATCCCACGTACGGCTGAATCTTCAGACTGGGGAAAGA; GAGGCAAAACTCCAATATGAGGACAAGTTCCGAAATAATTTGAAAGGCAAAAGGCTGGAT; ATCAACACCAACACCTACACATCTCAGGATCTCAAGAGTGCACTGGCAAAATTCAAGGAG; GGGGCAGAGATGGAGAGTTCAAAGGAAGACAAGGCAAGGCAGGCTGAGGTAAAGCGGCTC; TTCCGCCCCATTGAGGAACTGAAGAAAGACTTTGATGAGCTGAATGTTGTCATTGAGACT; GACATGCAGATCATGGTACGGCTGATCAACAAGTTCAATAGTTCCAGCTCCAGTTTGGAA; GAGAAGATTGCTGCGCTCTTTGATCTTGAATATTATGTCCATCAGATGGACAATGCGCAG; GACCTGCTTTCCTTTGGTGGTCTTCAAGTGGTGATCAATGGGCTGAACAGCACAGAGCCC; CTCGTGAAGGAGTATGCTGCGTTTGTGCTGGGCGCTGCCTTTTCCAGCAACCCCAAGGTC; CAGGTGGAGGCCATCGAAGGGGGAGCCCTGCAGAAGCTGCTGGTCATCCTGGCCACGGAG; CAGCCGCTCACTGCAAAGAAGAAGGTCCTGTTTGCACTGTGCTCCCTGCTGCGCCACTTC; CCCTATGCCCAGCGGCAGTTCCTGAAGCTCGGGGGGCTGCAGGTCCTGAGGACCCTGGTG; CAGGAGAAGGGCACGGAGGTGCTCGCCGTGCGCGTGGTCACACTGCTCTACGACCTGGTC; ACGGAGAAGATGTTCGCCGAGGAGGAGGCTGAGCTGACCCAGGAGATGTCCCCAGAGAAG; CTGCAGCAGTATCGCCAGGTACACCTCCTGCCAGGCCTGTGGGAACAGGGCTGGTGCGAG; ATCACGGCCCACCTCCTGGCGCTGCCCGAGCATGATGCCCGTGAGAAGGTGCTGCAGACA; CTGGGCGTCCTCCTGACCACCTGCCGGGACCGCTACCGTCAGGACCCCCAGCTCGGCAGG; ACACTGGCCAGCCTGCAGGCTGAGTACCAGGTGCTGGCCAGCCTGGAGCTGCAGGATGGT; GAGGACGAGGGCTACTTCCAGGAGCTGCTGGGCTCTGTCAACAGCTTGCTGAAGGAGCTG; AGATGA; ; Clone variation with respect to NM_022464.4; 153 a=>g;192 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022464 unedited; GCCCGCGTTTTGCGCAGGGGGATCTGGTCGCCGCCGCGGCCGCCTGGAACTGTGGGAGTT; GTGTCCGCCACTCGGCCTGCCGGAGGCCGAAGGTCCCTGACTATGGCCTCCCCAGAGCCT; GCCTTCATCTAGGACGGGCTCCTCTGGGCATGCTGCTTGGGCTGCTGATGGCCGCCTGCT; TCACCTTCTGCCTCATTCATCAGAACCTGAAGGAGTTTGCCCTGACCAACCCAGAAAAAG; CAGCACCAAAGAAACCGAGAAGAAAAAAAACCAAAGCCGAGGAAGAGCTGGATGCTGAAG; TCCTGGAGGTGTTCCACCCGACGCATGAGTGGCAGGCCCTTCAGCCAGGGCAGGCTGTCC; CTTGCAGATCCCACTTACCGCTGAATCTTCAGACTGGGGAAAGAGAGGCAAAACTCCAAT; ATGAGGACCAGTTCCGAAATAATTTGAAAGGCAAAAGGCTGGATATCAACACCAACACCT; ACACATCTCAGGATCTCAAGAGTGCACTGGCAAAATTCAAGGAGGGGGCAGAGATGGAGA; GTTCAAAGGAAGACAAGGCAAGGCAGGCTGAGGTAAAGCGGCTCTTCCGCCCCATTGAGG; AACTGAAGAAAGACTTTGATGAGCTGAATGTTGTCATTGAGACTGACATGCAGTTCATGG; TACGGCTGATCAACAAGTTCAATAGTTCCAGCTCCAGTTTGGAAGAGAAATTGCTGCGCT; CTTTGATCTTGAATATTATGTCCATCAGATGGACCAATGCGCAGACCTGCTTTCCTTTNG; NNTGTCTCAGTGGGTGATCAAATGGGCTGACAGCACAGAGCCCCTCGTGAANNGATATGC; TGCGTTTGTGCTGGGCGCTGCCTTTTNCAGCACCCCNAGGTCCAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022464 unedited; GACCGCGAGCCGCAATCTAGAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCC; TTCAGGTTTCCATTTAATGGCCAAGCCAGCACTGCCAAGATGTCCTCCTGCCTGAAAAGC; CCACCCACGCTGGCACCCCTCAACCTCACTAACGGCATCCCAGTCCAGTCCTGGTGGGGG; GCCTCATCTCAACTCCTTCAGCAAGCTGTTGACAGAGCCCAACAGCTCCTGGAAGTAGCC; CTCGTCCTCACCATCCTGCAGCTCCAGGCTGGCCAGCACCTGGTACTCAACCTGCAGGCT; GGCCAGTGTCCTGCCGAGCTGGGGGTCCTGACGGTAGCGGTCCCGGCAGGTGGTCAGGAG; GACGCCCAGTGTCTGCAGCACCTTCTCACGGGCATCATGCTCGGGCAGCGCCAGGAGGTG; GGCCGTGATCTCGCACCAACCCTGTTCCCACAGGCCTGGCAGGAGGTGTACCTGGCGATA; CTGCTGCAGCTTCTCTGGGGACATCTCCTGGGTCAGCTCAACCTCCTCCTCGGCGAACAT; CTTCTCCGTGACCAGGTCGTAGAGCAGTGTGACCACGCGCACGGNGAGCACCTTCGTGCC; CTTCTCCTGCACCAGGGTCCTCAGGACCTGCAGCCCCCCGAGCTTCAGGAACTGCCGCTG; GGCATAAGGGAAGTGGCGCACCAGGGAGCACAGTGCAAACAGGACCTTCTTCTTTGCAGT; GAGCGGCTGCTCCGCTGCCAGGATGACCACCACCTCCTGCAGGGCTCCCCCTTCGATGGG; CTNCACCTGGACTTTGGGTTGCTGGAAAAAGCAGNGCCCANCACAAACGCAGATACTCCT; TCACGAGGGCTCTGTGCTGTTCAGCCATTGATACCACCTGTAGACACCCAAGGAAAGCAG; GTCCTGCGCCATGCCATCTGATGAACTCATATTCAGATCAAAGACGCCACNATCTTCTCC; TTCAAATGGAGCCTGAACATTGCACCTTGTGTCAACCGAACATGATCGGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_022464
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022464.3, NP_071909.1
• RefSeq Size: 1815 bp
• RefSeq ORF: 1386 bp
• Locus ID: 64374
• UniProt ID: Q9H173
• Cytogenetics: 5q31.2
• Protein Families: Protease, Secreted Protein, Transmembrane
• Summary: This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks an exon in the 5'UTR compared to variant 1. Variants 1 and 2 encode the same protein.