PARP12 (NM_022750) Human Untagged Clone
$643.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | PARP12 |
| Synonyms | ARTD12; MST109; MSTP109; ZC3H1; ZC3HDC1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_022750 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_022750.2, NP_073587.1 |
| RefSeq Size | 3805 bp |
| RefSeq ORF | 2106 bp |
| Locus ID | 64761 |
| UniProt ID | Q9H0J9 |
| Cytogenetics | 7q34 |
| Domains | zf-CCCH |
| Summary | Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.UniProtKB/Swiss-Prot Function Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_022750" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209224 | PARP12 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 12 (PARP12) | 10 ug |
$642.00
|
|
| RC209224L1 | Lenti-ORF clone of PARP12 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 12 (PARP12) | 10 ug |
$942.00
|
|
| RC209224L2 | Lenti-ORF clone of PARP12 (mGFP-tagged)-Human poly (ADP-ribose) polymerase family, member 12 (PARP12) | 10 ug |
$942.00
|
|
| RC209224L3 | Lenti-ORF clone of PARP12 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 12 (PARP12) | 10 ug |
$942.00
|
|
| RC209224L4 | Lenti-ORF clone of PARP12 (mGFP-tagged)-Human poly (ADP-ribose) polymerase family, member 12 (PARP12) | 10 ug |
$942.00
|
|
| RG209224 | PARP12 (tGFP-tagged) - Human poly (ADP-ribose) polymerase family, member 12 (PARP12) | 10 ug |
$842.00
|
|
Citations
PARP1-produced poly-ADP-ribose causes the PARP12 translocation to stress granules and impairment of Golgi complex functions
Catara, Grimaldi, Schembri et al
Sci Rep (2017) 7 (1), 14035 DOI: 10.1038/s41598-017-14156-8
Catara, Grimaldi, Schembri et al
Sci Rep (2017) 7 (1), 14035 DOI: 10.1038/s41598-017-14156-8
AI Summary: OriGene’s Human PARP12 cDNA (pCMV6-XL5) was used to generate PARP12 expression constructs and mutants, enabling the study of PARP12’s role in stress granule translocation and Golgi complex function.
Snippet: …Human PARP12 cDNA was from Origene (for subcloning and mutations, see Supplementary. … His-tagged PARP12-Mutants were. generated from Human PARP12 cDNA from Origene as the pCMV6-XL5 using the oligonucleotides reported ...;... … Human PARP12 cDNA was from Origene (for subcloning and mutations, see Supplementary. … His-tagged PARP12-Mutants were. generated from Human PARP12 cDNA from Origene as the pCMV6-XL5 using the oligonucleotides reported
2,3,7,8-Tetrachlorodibenzo-p-dioxin poly(ADP-ribose) polymerase (TiPARP, ARTD14) is a mono-ADP-ribosyltransferase and repressor of aryl hydrocarbon receptor transactivation
MacPherson, Tamblyn, Rajendra et al
Nucleic Acids Res. (2013) 41 (3), 1604-21 DOI: 10.1093/nar/gks1337
MacPherson, Tamblyn, Rajendra et al
Nucleic Acids Res. (2013) 41 (3), 1604-21 DOI: 10.1093/nar/gks1337
Cited by 180
AI Summary: OriGene’s pCMV-XL-PARP-1 and pCMV-XL-PARP-12 expression plasmids were used in this study to investigate the catalytic functions of PARP family members, helping to elucidate TiPARP’s role as a mono-ADP-ribosyltransferase and repressor of aryl hydrocarbon receptor transactivation.
Snippet: Gardner) or 200 ng pcDNA-mTiparp, pcDNA-chTiPARP, 200–1500 ng pCMV-XL-PARP-1 or pCMV-XL-PARP-12 (Origene) or 200 ng ... Membranes were incubated with anti-AHR (Enzo Life Sciences Inc., SA-210) overnight and then incubated for 1 h with anti-rabbit horseradish
2,3,7,8-TETRACHLORODIBENZO-p-DIOXIN-INDUCIBLE POLY(ADP-RIBOSE) POLYMERASE IS A MONO-ADPRIBOSYLTRANSFERASE AND A LIGAND-INDUCED REPRESSOR OF AHR TRANSACTIVATION
MacPherson
Thesis (2014)
MacPherson
Thesis (2014)
Snippet: All full-length human TiPARP expression constructs, TiPARP N-terminal truncation expression constructs and the C-terminal truncation 1-234 were created by PCR amplification of TiPARP cDNA from pCMV6-XL4-hTiPARP (Origene, Rockwood, MD). Two-hundred micrograms of pCMV6-XL4-hTiPARP was added to a reaction mixture containing 0.2 μM of desired forward primer containing Kozak sequence and/or EcoRI r...
Showing 1-3 of 3 papers.
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