Apoptosis enhancing nuclease (AEN) (NM_022767) Human Untagged Clone

SKU SC112416

AEN (untagged)-Human apoptosis enhancing nuclease (AEN)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Apoptosis enhancing nuclease
• Synonyms: ISG20L1; pp12744
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022767, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGTACCCCGGGAGGCCCCTGAGTCTGCTCAGTGCCTGTGCCCTTCCCTCACCATCCCAAATGCCAAGG; ATGTGCTTCGGAAGAGGCACAAGAGAAGGAGCCGACAGCACCAGCGGTTCATGGCCCGGAAGGCCTTGCT; GCAGGAGCAGGGGCTGCTGAGCATGCCTCCAGAACCAGGGTCCTCCCCACTGCCCACCCCTTTCGGGGCA; GCGACAGCAACTGAAGCTGCCAGCAGTGGGAAGCAGTGTCTGAGGGCTGGATCTGGCAGTGCCCCATGCA; GCAGAAGGCCTGCTCCCGGGAAAGCCTCAGGGCCCTTGCCCAGCAAGTGTGTGGCTATCGACTGTGAGAT; GGTGGGCACGGGACCCCGAGGGCGGGTAAGCGAGCTGGCCCGCTGTTCCATTGTGAGCTACCATGGCAAT; GTCCTCTATGACAAGTACATCAGGCCTGAGATGCCCATCGCTGACTACCGTACCCGCTGGAGTGGCATCA; CTCGGCAGCACATGCGCAAGGCTGTCCCCTTCCAGGTGGCCCAGAAAGAGATCCTTAAGCTCCTGAAGGG; CAAGGTGGTGGTGGGGCACGCGCTGCACAACGACTTCCAGGCGCTCAAGTATGTCCACCCTCGGAGCCAG; ACCCGGGATACGACCTATGTCCCAAACTTCCTCAGCGAGCCCGGCCTCCACACCCGGGCCCGGGTCTCTC; TAAAGGACCTGGCCCTGCAGCTGCTGCACAAGAAGATCCAGGTGGGCCAGCACGGGCACTCATCAGTAGA; AGATGCCACGACAGCCATGGAGCTCTACCGGCTGGTGGAGGTGCAGTGGGAACAGCAGGAGGCCCGCAGC; CTCTGGACCTGCCCCGAGGACAGAGAACCTGACAGCAGCACAGACATGGAACAGTACATGGAGGACCAGT; ACTGGCCCGATGACCTGGCCCACGGCAGCAGAGGAGGAGCCAGGGAGGCACAGGACAGAAGGAATTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022767 unedited; NGGGGGTGTTCAGNAATTTATGTNAATACGAACTNCACTTTTAGGGNNNCGGCCGCGNNA; ATTCGGCACGNAGGGATNNCTGGCNNAGTGCCCCATGNNCAGCAGNAAGGGCCTGGCTCC; CGGGNAAAGCCTCAGGGCCCTTGCCCAGCAAGTGTGTGGCTATCGACTGTGAGATGGTGG; GCACGGGACCCCGAGGGCGGGTAAGCGAGCTGGCCCGCTGTTCCATTGTGAGCTACCATG; GCGATGTCCTCTATGACAAGTACATCAGGCCTGAGATGCCCATCGCTGACTACCGTACCC; GCTGGAGTGGCATCACTCGGCAGCACATGCGCAAGGCTGTCCCCTTCCAGGTGGCCCAGA; AAGAGATCCTTAAGCTCCTGAAGGGCAAGGTGGTGGTGGGGCACGCGCTGCACAACGACT; TCCAGGCGCTCAAGTATGTCCACCCTCGGAGCCAGACCCGGGATACCACCTATGTCCCAA; ACTTCCTCAGCGAGCCCGGCCTCCACACCCGGGCCCGGGTCTCTCTAAAGGACCTGGCCC; TGCAGCTGCTGCACAAGAAGATCCAGGTGGGCCAGCACGGGCACTCATCAGTAGAAGATG; CCACGACAGCCATGGAGCTCTACCGGCTGGTGGAGGTGCAGTGGGAACAGCAGGAGGCCC; GCAGCCTCTGNGACCTGCCCCGAGACAGAGAACCTGACAGCAGCACAGACATGGAACAGT; ACATGGAGGACCAGTACTGGCCCGATGACCTGGCCCACGGCAGCAGAGGAGGAGCCAGGG; AGGCACAGGACAGAAAGAATTGAGAAAGGGGCGGGGCTCCCTGGCTGGGCTTNCNGTGTG; GCCGGTAAGAAGTGGGGGCCAGAGAGCAGCGGGCACTCCTTCCTGGGCAGGNTGGNGCAN; GATGCANTGAGCCCGCCCCAGGCCAGNAGAGTTGGGGTCATCTGTTACCTTGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022767 unedited; TTGTACCGCGGCCGCTTCTAAATCGAGTTTTTTTTTTTTTTTTTTCACGTTTCCAATTGA; GTTTTATTCTAGAACAAATGGTTTTACAACAAAGCTGATCTTAAATAGTACATTTCCCCG; CTGATAACAGCGCCATCTTCCTTCGACCCCCAGACCAAGGGATCAAAGCTACTGCTCAAT; TGAGTTTGACATCAGCTACCGACTTTTAAGGAATCTAGAACAAAGTTCTGGAGCCAGGCC; AGAAGTGGTAAAAGATTCAGTTAGCCCCTTCCTCTGTGCTGTGCTGACAGGGAGGGAGCC; AGTGACTGGACAATCTATGACCCCAAGTTTGTGGAACGTGGGGCTTCAGCTGAGGGTGGG; GTAGTGTGATTTCAGCAGTGTCTCCACCTCCCTGACCCCTGCGCTGCTTTGCTCTGTTGT; CACCTCCCGCACAGTGTCGGGACACTGACCGGAAGCCAAACCTGTGCCTGGTGGCCACAG; ACAAGACACACGGATCTCCGTGAACCTTGCTGTTGTGCCCTTACCGAGGCCATTGTGCTA; CCTGTTGAACAATAAAACCGGTCCTATTTGACTGCGCCCCCATTGTTCAACTGTGACCTC; TCGTGTACTCCGCAAAAATGCTTCTATATCTGAGGGATACGCCTAGGCTTTTCAATCCAC; TACGCGGGAACCGCGTATCTCCATACATTCCCGTCTTCCTGACCTACTCCCTGTCCCCCT; CCAGTACAACCCCGTACACATATCTCCCAACATCCCGCACACCTCGTCCACTAGTCTCCC; TATCCCCTCCTCCCCCTCGCAGCCTTCTTCCTTCATTCATCTTGCGTTACTTCGTGTTTT; CTAGCAATCTCCCTCTATAGCACCGCTCCTTCCCATATCCCCCCCATTACCTCTCATCCC; TCTAACATCCGTTACTTCTCAGTTCTTATTCCCACCCTTGATCCACGTACAATATTACCA; TCTCATCATAAAATATTATN;
• Restriction Sites: NotI-NotI
• ACCN: NM_022767
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022767.1, NP_073604.1
• RefSeq Size: 3134 bp
• RefSeq ORF: 630 bp
• Locus ID: 64782
• UniProt ID: Q8WTP8
• Cytogenetics: 15q26.1
• Domains: EXOIII
• Summary: Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis.[UniProtKB/Swiss-Prot Function]