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TSEN34 (NM_024075) Human Untagged Clone

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SKU
SC112314
TSEN34 (untagged)-Human tRNA splicing endonuclease 34 homolog (S. cerevisiae) (TSEN34), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TSEN34
Synonyms LENG5; PCH2C; SEN34; SEN34L
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC112314 sequence for NM_024075 edited (data generated by NextGen Sequencing)
ATGCTGGTGGTGGAGGTGGCGAACGGCCGCTCCCTGGTGTGGGGAGCCGAGGCGGTGCAG
GCCCTCCGGGAGCGCCTGGGTGTGGGGGGCCGCACGGTAGGCGCCCTGCCCCGCGGGCCC
CGCCAGAACTCGCGCCTGGGCCTCCCGCTGCTGCTGATGCCCGAAGAGGCGCGGCTCTTG
GCCGAGATCGGCGCCGTGACTCTGGTCAGCGCCCCGCGTCCAGACTCTCGGCACCACAGC
CTGGCCCTGACATCCTTCAAGCGCCAGCAAGAGGAGAGCTTCCAGGAGCAGAGCGCCTTG
GCAGCTGAGGCCCGGGAGACCCGTCGTCAGGAGCTCCTGGAGAAGATTACGGAGGGCCAG
GCTGCTAAGAAGCAGAAACTAGAACAGGCTTCAGGGGCCAGCTCAAGCCAGGAGGCCGGC
TCGAGCCAGGCTGCCAAAGAGGATGAGACCAGTGATGGCCAGGCTTCGGGAGAGCAGGAG
GAAGCTGGCCCCTCGTCTTCCCAAGCAGGACCCTCAAATGGGGTAGCCCCCTTGCCCAGA
TCTGCTCTCCTTGTCCAGCTGGCCACTGCCAGGCCTCGACCGGTCAAGGCCAGGCCCCTG
GACTGGCGTGTCCAGTCTAAAGACTGGCCCCACGCCGGCCGCCCTGCCCACGAGCTGCGC
TACAGTATCTACAGAGACCTGTGGGAGCGAGGCTTCTTCCTCAGTGCGGCTGGCAAGTTC
GGAGGTGACTTCCTGGTCTATCCTGGTGACCCCCTCCGCTTCCACGCCCATTATATCGCT
CAGTGCTGGGCCCCTGAGGACACCATCCCACTCCAAGACCTGGTTGCTGCTGGGCGCCTT
GGAACCAGCGTCAGAAAGACCCTGCTCCTCTGTTCTCCGCAGCCTGATGGTAAGGTGGTC
TACACCTCCCTGCAATGGGCCAGCCTGCAGTGA

Clone variation with respect to NM_024075.3
795 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_024075 unedited
TGGCATTTGTAATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGGTTGGGTACA
AGTGACGCTAGGATGATAGGCGGAGTCAACAGGTTCGCCAGACACCCATGAGTATTTACA
AGGGGGCGGGGCGAAAGCGACTTGCCCTCAAAGGGCGGAACCCCGAGGGCCGGCGTGCGC
CTACGGGACCGGGCCAGGATGACGATCCTCAAGTTCCCAAGTAGAGGAGAGGAAGCGGCA
GAGGGAGGAGGATGCTGGTGGTGGAGGTGGCGAACGGCCGCTCCCTGGTGTGGGGAGCCG
AGGCGGTGCAGGCCCTCCGGGAGCGCCTGGGTGTGGGGGGCCGCACGGTAGGCGCCCTGC
CCCGCGGGCCCCGCCAGAACTCGCGCCTGGGCCTCCCGCTGCTGCTGATGCCCGAAGAGG
CGCGGCTCTTGGCCGAGATCGGCGCCGTGACTCTGGTCAGCGCCCCGCGTCCAGACTCTC
GGCACCACAGCCTGGCCCTGACATCCTTCAAGCGCCAGCAAGAGGAGAGCTTCCAGGAGC
AGAGCGCCTTGGCAGCTGAGGCCCGGGAGACCCGTCGTCAGGAGCTCCTGGAGAAGATTA
CGGAGGGCCAGGCTGCTAAGAAGCAGAAACTAGAACAGGCTTCAGGGGCCAGCTCAAGCC
AGGAGGCCGGCTCGAGCCAGGCTGCCAAAGAGGATGAGACCAGTGATGGCCAGGCTTCGG
GAGAGCAGGANGAAGCTGGCCCCTCGTCTTCCCAAGCAGGACCCTCAAATGGNGTAGNCC
CCTTGCCCAGATCTGCTCTCCTTGNTCAGCTGGCCACTGNCCAGCCTCGACCGGTCAGGG
GCCAGCCCTGGACTGGCGTGTCCAGTCTAAGACTGGNCCCACGCCGGNCGNNCCTGCCAG
AGCTGCGCTACATATTACAGAGACTGTGGGAGCGAGGCTTCTTNCTCATGCGCTGGCAAG
TCGNAGTGACTTCTG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_024075 unedited
GGGCCATTAGAACGCGGCCGCATTTTANGATCGGTTTTTTTTTTTTTTTTTTAACATTTA
TATACCAAGTTTATTGGAAACCTCCTGAGACTTAAACCCTGTAGAAAACAGATGAATCAA
ACCCAGGCCCCATTCTCGGGAAGCTCACAGTGTGAAAATAGGTAACTACAAAAACACAGC
CAATAAGTGCTTTGAAACAAGGAAGAACATAAAAAAGATGTAGTGTTCGAAAACCTGGAA
TCAAAAACTAACCGCGGAGAAAGGTAGGAGGATGTTCTAGACTCCCAGAGAAGAGCTGGG
GAACATCCAGAAAGGCTCTTGCTGCCGACACAGCCACATCCCCTAGGTCTCTGGAGTTCA
CTGCAGGCTGGCCCATTGCAGGGAGGTGTAGACCACCTTACCATCAGGCTGCGGAGAACA
GAGGAGCAGGGTCTTTCTGACGCTGGTTCCAAGGCGCCCAGCAGCAACCAGGTCTTGGAG
TGGGATGGTGTCCTCAGGGGCCCAGCACTGAGCGATATAATGGGCGTGGAAGCGGAGGGG
GTCACCAGGATAGACCAGGAAGTCACCTCCGAACTTGCCAGCCGCACTGAGGAAGAAGCC
TCGCTCCCACAGGTCTCTGTAGATACTGTAGCGCAGCTCGTGGGCAGGGCGGCCGGCGTG
GGGCCAGTCTTTAGACTGGACACGCCAGTCCAGGGGCCTGGCCTTGACCGGTCGAGGCCT
GGCAGTGGCCAGCTGGACAAGAGAGCAGATCTGGGCAGGGGGCTACCCCATTTGAGGTCC
TGCTTGGGAAGAGATGGGCCAGCTTNCTNCTGCTCTCCGGAGCCTGGCCATCACTGGTCT
CATCCTCTTTGGCAGCCTGGCTCGAGCCGGCCTCCTGGCTTGAGCC
Restriction Sites NotI-NotI |
ACCN NM_024075
Insert Size 1650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024075.1, NP_076980.1
RefSeq Size 1364 bp
RefSeq ORF 1173 bp
Locus ID 79042
UniProt ID Q9BSV6
Cytogenetics 19q13.42
Domains tRNA_int_endo
Summary This gene encodes a catalytic subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from precursor tRNAs. The endonuclease complex is also associated with a pre-mRNA 3-prime end processing factor. A mutation in this gene results in the neurological disorder pontocerebellar hypoplasia type 2. Multiple alternatively spliced variants, encoding the same protein, have been identified.provided by RefSeq, Oct 2009
Transcript Variant: This variant (1) encodes the shorter isoform (1). Variants 1, 2, and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_024075" in other vectors (6)
SKU Description Size Price
RC200954 TSEN34 (Myc-DDK-tagged)-Human tRNA splicing endonuclease 34 homolog (S. cerevisiae) (TSEN34), transcript variant 1 10 ug
$300.00
RC200954L1 Lenti ORF clone of Human tRNA splicing endonuclease 34 homolog (S. cerevisiae) (TSEN34), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200954L2 Lenti ORF clone of Human tRNA splicing endonuclease 34 homolog (S. cerevisiae) (TSEN34), transcript variant 1, mGFP tagged 10 ug
$600.00
RC200954L3 Lenti ORF clone of Human tRNA splicing endonuclease 34 homolog (S. cerevisiae) (TSEN34), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200954L4 Lenti ORF clone of Human tRNA splicing endonuclease 34 homolog (S. cerevisiae) (TSEN34), transcript variant 1, mGFP tagged 10 ug
$600.00
RG200954 TSEN34 (tGFP-tagged) - Human tRNA splicing endonuclease 34 homolog (S. cerevisiae) (TSEN34), transcript variant 1 10 ug
$500.00

file-alt Citations

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