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SPTLC1 (NM_006415) Human Untagged Clone

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SKU
SC111752
SPTLC1 (untagged)-Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SPTLC1
Synonyms HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111752 sequence for NM_006415 edited (data generated by NextGen Sequencing)
ATGGCGACCGCCACGGAGCAGTGGGTTCTGGTGGAGATGGTACAGGCGCTTTACGAGGCT
CCTGCTTACCATCTTATTTTGGAAGGGATTCTGATCCTCTGGATAATCAGACTTCTTTTC
TCTAAGACTTACAAATTACAAGAACGATCTGATCTTACAGTCAAGGAAAAAGAAGAACTG
ATTGAAGAGTGGCAACCAGAACCTCTTGTTCCTCCTGTCCCAAAAGACCATCCTGCTCTC
AACTACAACATCGTTTCAGGCCCTCCAAGCCACAAAACTGTGGTGAATGGAAAAGAATGT
ATAAACTTCGCCTCATTTAATTTTCTTGGATTGTTGGATAACCCTAGGGTTAAGGCAGCA
GCTTTAGCATCTCTAAAGAAGTATGGCGTGGGGACTTGTGGACCCAGAGGATTTTATGGC
ACATTTGATGTTCATTTGGATTTGGAAGACCGCCTGGCAAAATTTATGAAGACAGAAGAA
GCCATTATATACTCATATGGATTTGCCACCATAGCCAGTGCTATTCCTGCTTACTCTAAA
AGAGGGGACATTGTTTTTGTAGATAGAGCTGCCTGCTTTGCTATTCAGAAAGGATTACAG
GCATCCCGTAGTGACATTAAGTTATTTAAGCATAATGACATGGCTGACCTCGAGCGACTA
CTAAAAGAACAAGAGATCGAAGATCAAAAGAATCCTCGCAAGGCTCGTGTAACTCGGCGT
TTCATTGTAGTAGAAGGATTGTATATGAATACTGGAACTATTTGTCCTCTTCCAGAATTG
GTTAAGTTAAAATACAAATACAAAGCAAGAATCTTCCTGGAGGAAAGCCTTTCATTTGGA
GTCCTAGGAGAGCATGGCCGAGGAGTCACTGAACACTATGGAATCAATATTGATGATATT
GATCTTATCAGTGCCAACATGGAGAATGCACTTGCTTCTATTGGAGGTTTCTGCTGTGGC
AGGTCTTTTGTAATTGACCATCAGCGACTTTCCGGCCAGGGATACTGCTTTTCAGCTTCG
TTACCTCCCCTGTTAGCTGCTGCAGCAATTGAGGCCCTCAACATCATGGAAGAGAATCCA
GGTATTTTTGCAGTGTTGAAGGAAAAGTGCGGACAAATTCATAAAGCTTTACAAGGCATT
TCTGGATTAAAAGTGGTGGGGGAGTCCCTTTCTCCAGCCTTTCACCTACAACTGGAAGAG
AGCACTGGGTCTCGCGAGCAAGATGTCAGACTGCTTCAGGAAATTGTAGATCAATGCATG
AACAGAAGTATTGCATTAACTCAGGCGCGCTACTTGGAGAAAGAAGAGAAGTGTCTCCCT
CCTCCCAGCATTCGGGTTGTGGTCACGGTGGAACAAACAGAGGAAGAACTGGAGAGAGCT
GCGTCCACCATCAAGGAGGTAGCCCAGGCCGTCCTGCTCTAG

Clone variation with respect to NM_006415.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006415 unedited
GGGTTCANAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCTAACTAT
GGCGACCGCCACGGAGCAGTGGGTTCTGGTGGAGATGGTACAGGCGCTTTACGAGGCTCC
TGCTTACCATCTTATTTTGGAAGGGATTCTGATCCTCTGGATAATCAGACTTCTTTTCTC
TAAGACTTACAAATTACAAGAACGATCTGATCTTACAGTCAAGGAAAAAGAAGAACTGAT
TGAAGAGTGGCAACCAGAACCTCTTGTTCCTCCTGTCCCAAAAGACCATCCTGCTCTCAA
CTACAACATCGTTTCAGGCCCTCCAAGCCACAAAACTGTGGTGAATGGAAAAGAATGTAT
AAACTTCGCCTCATTTAATTTTCTTGGATTGTTGGATAACCCTAGGGTTAAGGCAGCAGC
TTTAGCATCTCTAAAGAAGTATGGCGTGGGGACTTGTGGACCCAGAGGATTTTATGGCAC
ATTTGATGTTCATTTGGATTTGGAAGACCGCCTGGCAAAATTTATGAAGACAGAAGAAGC
CATTATATACTCATATGGATTTGCCACCATAGCCAGTGCTATTCCTGCTTACTCTAAAAG
AGGGGACATTGTTTTTGTAGATAGAGCTGCCTGCTNTGCTATTCAGAAAGGATTACAGGC
ATCCCGTAGTGACATTAAGTTATTTAAGCATAATGACATGGCTGACCTCGAGCGACTACT
AAAAGAACCAAGAGATCGAGATCAAAAGAATCCTCGCAGGGCTCGTGTAACTCGGCGTTT
CATTGTAGTAGAAGGATTGTATATGAATACTGGAACTATTTGTCCTCTTCCAGAAATTGG
TAAGTTAAAATACANATTCAAAGCAAGAATCTTTCTGGNAGGAAAGNCCTTTCATTTGNN
AGTCCCTAGAGAGCATGGNCCGAGNAGTCACTGNACN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006415 unedited
ACCGCGGGCCGCTTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAGGAATTAGGTTTAT
TTCATTAAAAAATAAAACAAAACAAACCAGACTCATCCCCCAAAGAGTACTTTATCCTTT
AAAGCAGCAGCAATCACAAGGCACATATGTACAAAATACCTCAAGCAAAATAGAAACTGA
ACATTCAAAAAGTATGTGTTGTTTAAAAAGATGTTGAAGACCATTTGAGTAATAAGCACA
GGTTATAAAAATCACAATTTTATTCTTCTACCTTTAACAAGAGAAGGAATGATGGCATAA
TATTTATATGCTATATTGTGGCACGTATAACAAACCTTTACACCACATAACCTGGTTTGC
TAAAGAAAAAACAGGTAAAGTACTGCTTAAAAATGGTATTTTTAGTGCTTTCACATCTTT
TGTAGATATCTGGAATTTGTAAGATGAAAAGAAAAATTCTCTTTTTCCTTGGCTTAGTAA
AAGCCTGTAGTTACACGTCCCTAGGCAAACCCACCATACAAAAAAGGAATACATTTGAGA
TATTCTAAAATATAAAGGATATGATTTATATTATGGGATCAACATAATAACCAACTCTCT
TCCTAAAACAGATTAGAGGTGAACACATAAGAAATGTAAAGAAAGGTTAATTACATTAAA
ATAAAATAGCTTCTGATTTAAATTATTGCTCACTTAGGAAGTATAATGAATATTCTCTGG
CTAAGGATGCTCAGATTAGAAATGGTGTGACATCAATATTTAGTGTGTACAAAAAGTCTA
AGATTAAATACATTGCANAGTTATAAATAATCACACAAAAGTGTCCTTTTAGCCACCATT
TAGCTCACACAACGAAGACTGGAAAATACGTGGTTATTTAGATCTTCATATAAAATTGTA
CACATATGTTGAAGTGTCCTCCTAGCTTTATGTTGCAGTCATTTTCTAATGAAAT
Restriction Sites NotI-NotI |
ACCN NM_006415
Insert Size 3010 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006415.2, NP_006406.1
RefSeq Size 2780 bp
RefSeq ORF 1422 bp
Locus ID 10558
UniProt ID O15269
Cytogenetics 9q22.31
Domains aminotran_1_2
Protein Families Druggable Genome, Transmembrane
Protein Pathways Metabolic pathways, Sphingolipid metabolism
Summary This gene encodes a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13. provided by RefSeq, Jul 2013
Transcript Variant: This variant (1) represents the longest transcript, and encodes isoform a.
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Documents
Resources
"NM_006415" in other vectors (6)
SKU Description Size Price
RC222985 SPTLC1 (Myc-DDK-tagged)-Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1 10 ug
$457.00
RC222985L1 Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC222985L2 Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC222985L3 Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC222985L4 Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG222985 SPTLC1 (tGFP-tagged) - Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1 10 ug
$489.00 $657.00

file-alt Citations

Expression of the ORMDLS, Modulators of Serine Palmitoyltransferase, is Regulated by Sphingolipids in Mammalian Cells
Gupta, Gable, Alexaki et al
J. Biol. Chem. (2014)
AI Summary: OriGene products contributed to this study by providing plasmids containing human LCB1 and LCB2a cDNAs, as well as a C-terminally tagged ORMDL1-GFP in the pcMV6-AC-GFP vector, which were used to investigate the regulation of sphingolipid biosynthesis in mammalian cells.
Snippet: Plasmids containing the human LCB1 and LCB2a cDNAs under the control of CMV promoter were purchased from OriGene Technologies, Inc. ... The C-terminally tagged ORMDL1-GFP in the pcMV6-AC-GFP vector was purchased from OriGene Technologies, Inc. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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