RFC5 (NM_007370) Human Untagged Clone

SKU SC111685

RFC5 (untagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RFC5
• Synonyms: RFC36
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007370, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAGACCTCAGCACTCAAGCAGCAGGAGCAGCCCGCGGCGACCAAGATCAGGAACCTGCCCTGGGTTG; AAAAATACCGGCCACAGACCCTGAATGATCTCATTTCTCATCAGGACATTCTGAGTACCATTCAGAAGTT; TATCAATGAAGACCGACTGCCACACTTGCTTCTCTACGGTCCCCCAGGGACAGGCAAGACATCTACCATC; CTAGCCTGTGCGAAACAGCTATATAAAGACAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCAG; ATGACCGAGGAATAGACATCATTCGAGGACCGATCCTGAGCTTTGCTAGCACAAGGACAATATTTAAGAA; AGGCTTTAAGCTAGTGATCTTGGATGAAGCAGACGCCATGACTCAGGACGCCCAGAATGCCTTGAGAAGA; GTAATTGAGAAATTCACAGAAAATACCAGATTCTGCCTCATCTGTAACTATCTGTCAAAGATCATCCCTG; CCTTGCAGTCCCGCTGCACGAGGTTTCGGTTCGGTCCCCTGACTCCTGAACTCATGGTTCCCCGCCTGGA; ACATGTCGTGGAAGAAGAGAAAGTTGATATAAGTGAAGATGGAATGAAAGCACTAGTCACTCTTTCCAGT; GGAGACATGCGTAGGGCTCTGAACATTTTGCAGAGCACCAATATGGCCTTTGGGAAGGTGACAGAGGAGA; CTGTCTACACCTGCACCGGGCACCCGCTCAAGTCAGACATTGCCAACATCCTGGACTGGATGTTGAATCA; AGATTTCACCACAGCCTACAGAAATATTACAGAGTTGAAAACTCTGAAGGGGTTGGCACTGCATGATATC; CTGACAGAGATACACTTGTTTGTGCATAGAGTTGACTTTCCATCTTCAGTTCGAATACATTTATTGACCA; AAATGGCAGACATTGAGTACAGGCTTTCTGTTGGCACCAACGAGAAGATCCAGCTGAGCTCCCTCATTGC; TGCATTTCAAGTCACCAGAGACCTGATTGTTGCAGAGGCCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007370 unedited; GTTCGCATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCTCAGGGTCA; GGTCGCGGCTGGTCACTGTGCAGGCGCTTGGGTGACGCGACGATCTCAGCGGATCTGGTC; ACCTTCGTCTCCCCGCCATGGAGACCTCAGCACTCAAGCAGCAGGAGCAGCCCGCGGCGA; CCAAGATCAGGAACCTGCCCTGGGTTGAAAAATACCGGCCACAGACCCTGAATGATCTCA; TTTCTCATCAGGACATTCTGAGTACCATTCAGAAGTTTATCAATGAAGACCGACTGCCAC; ACTTGCTTCTCTACGGTCCCCCAGGGACAGGCAAGACATCTACCATCCTAGCCTGTGCGA; AACAGCTATATAAAGACAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCAGATG; ACCGAGGAATAGACATCATTCGAGGACCGATCCTGAGCTTTGCTAGCACAAGGACAATAT; TTAAGAAAGGCTTTAAGCTAGTGATCTTGGATGAAGCAGACGCCATGACTCAGGACGCCC; AGAATGCCTTGAGAAGAGTAATTGAGAAATTCACAGAAAATACCAGATTCTGCCTCATCT; GTAACTATCTGTCAAAGATCATCCCTGCCTTGCAGTCCCGCTGCACGAAGTTTCGGGTCC; GGCCCCTGACTCCTGGACTCATGGTTCCCCCGCTGGAACATGTCGTGGAAAGAGAGAAAA; TTGATATTAGTGAAAAACGGAAGGAAAGCCCTCATCCCTTTTTTCCAGGGGAAACAGGCC; TAGGGGCTCTGAAAATTTTGCAGAACCCCCATATGGCCTTTTGGGAAGGGTGACAAGAGA; AACTGGTTTAACTTGCACCGGCCCCCCCGTTAAGACAACATCGTCCAACATCTGGGACCG; GTGGCTGAAAACG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007370 unedited; GAAATTTTGNCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTA; AAAAACAGCATCTAAAGAAAGTTTATTCTGTGATATTAGAGACAGAAGGTTTTCATATAA; ATGCAAGTTTGACAAAGTCAGCATCTTTCTAGCTGTCTAAGGAAGAGTCACTTGTAACAC; AGCCAGCCAGGAGGCTGCTTTGTTTTTTATTATAAAGAACACTAACACAAATGCAGCATG; ATTGCTGTAAAATAAATGTGAAATTTGTACAAAAGTCCCAGTCCTTCCGCAGTTCTAGGT; TTACAGTCAGGCTCAACCTTACTTGCCCCGCTCCTGCATGAAAACAAGTGCGTTTTATAC; AGCCTCTGCCACCCAGTGGCATAGTTTAAGGCTCTATATTATATTTAAAATTAAGGTTTT; TTTCCTATCATACACATCAATTTGGAACCTGCTGGTGACTCTTCTACCACAGAGGGTAAA; TAAAAAGACCAGTTTTTAAAGTAAGAGGATTCAGCTCATCTTATAAAAGTATAATAGTTT; CGGCCAGACGTCCATTAACATCTGTTTAAATTAGATACAGACACAATGCCAAATATTAGA; ATCATAAATGTAATTTTGGAGATAATGTTTACCTTGAGTAGGAAGACACTTGGACCATAT; TAAATTTGAAAGTCTTCTGTTCCAGACCAAAATGGGGTAGGCTAATTCCCTGTCATCCAA; GCAACTAAAAGGTAAAAACCTTATAACTTTAAAATAAAAAAGGTTATTTTTTTTCCCCTT; ATAAAGACAGGCAGTATGAGTTAATACATTAAAATTTATTTTGTACATCCCTGCTCCAAA; CACCACAAAAATGTACTTTTTTAATGCCTGNCCATCCTCTNCTGGAAGGGGTTTTTNTCA; GATTCNGGGTGACTGATTCATTCCACAGCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_007370
• Insert Size: 2140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007370.3, NP_031396.1
• RefSeq Size: 2119 bp
• RefSeq ORF: 1023 bp
• Locus ID: 5985
• UniProt ID: P40937
• Cytogenetics: 12q24.23
• Domains: AAA
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Summary: This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]; Transcript Variant: This variant (1) encodes the longest isoform (1).