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PPEF1 (NM_006240) Human Untagged Clone

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SKU
SC111671
PPEF1 (untagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1
  $609.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PPEF1
Synonyms PP7; PPEF; PPP7C; PPP7CA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111671 sequence for NM_006240 edited (data generated by NextGen Sequencing)
ATGGGATGCAGCAGTTCTTCAACGAAAACCAGGAGATCTGACACATCACTGAGAGCTGCG
TTGATCATCCAGAACTGGTACCGAGGTTACAAAGCTCGACTGAAGGCCAGACAACACTAT
GCCCTCACCATCTTCCAGTCCATCGAATATGCTGATGAACAAGGCCAAATGCAGTTATCC
ACCTTCTTTTCCTTCATGTTGGAAAACTACACACATATACATAAGGAAGAGCTAGAATTA
AGAAATCAGTCTCTTGAAAGCGAACAGGACATGAGGGATAGATGGGATTATGTGGACTCG
ATAGATGTCCCAGACTCCTATAATGGTCCTCGGCTACAATTTCCTCTCACTTGTACGGAT
ATTGATTTACTTCTTGAGGCCTTCAAGGAACAACAGATACTTCATGCCCATTATGTCTTA
GAGGTGCTATTTGAAACCAAGAAAGTCCTGAAGCAAATGCCGAATTTCACTCACATACAA
ACTTCTCCCTCCAAAGAGGTAACAATCTGTGGTGATTTGCATGGGAAACTGGATGATCTT
TTTTTGATCTTCTACAAGAATGGTCTCCCCTCAGAGAGGAACCCGTATGTTTTTAATGGT
GACTTTGTAGATCGAGGAAAGAATTCCATAGAGATCCTAATGATCCTGTGTGTGAGTTTT
CTTGTCTACCCCAATGACCTGCACTTGAACAGAGGGAACCACGAAGATTTTATGATGAAT
CTGAGGTATGGCTTCACGAAAGAAATTTTGCATAAATATAAGCTACATGGAAAAAGAATC
TTACAAATCTTGGAAGAATTCTATGCCTGGCTCCCAATCGGTACAATCGTTGACAATGAA
ATCCTGGTCATCCATGGTGGGATATCAGAGACCACAGACTTGAATTTACTCCACCGTGTA
GAGAGGAACAAGATGAAATCTGTGCTGATACCACCAACGGAAACAAACAGAGACCATGAC
ACTGACTCGAAGCACAATAAAGTAGGTGTGACTTTTAATGCACATGGAAGAATCAAAACA
AATGGATCTCCTACTGAACACTTAACAGAGCATGAATGGGAACAGATTATTGATATTCTG
TGGAGTGATCCCAGAGGCAAAAATGGCTGTTTTCCAAATACGTGCCGAGGAGGGGGCTGC
TATTTTGGACCAGATGTTACTTCCAAGATTCTTAATAAATACCAGTTGAAGATGCTCATC
AGGTCTCATGAATGTAAGCCCGAAGGGTATGAAATCTGTCATGATGGGAAGGTGGTGACT
ATATTTTCTGCTTCTAATTATTATGAAGAAGGCAGCAATCGAGGAGCTTACATCAAACTA
TGTTCTGGTACAACTCCTCGATTTTTCCAGTACCAAGTAACTAAAGCAACGTGCTTTCAG
CCTCTTCGCCAAAGAGTGGATACTATGGAAAACAGCGCCATCAAGATATTAAGAGAGAGA
GTGATTTCACGAAAAAGTGACCTTACTCGTGCTTTCCAACTTCAAGACCACAGAAAATCA
GGAAAACTTTCTGTGAGCCAGTGGGCTTTTTGCATGGAGAACATTTTGGGGCTGAACTTA
CCATGGAGATCCCTCAGTTCGAATCTGGTAAACATAGACCAAAATGGAAACGTTGAATAC
ATGTCCAGCTTCCAGAATATCCGCATTGAAAAACCTGTACAAGAGGCTCATTCTACTCTA
GTTGAAACTCTGTACAGATACAGATCTGACCTGGAAATCATATTTAATGCCATTGACACT
GATCACTCAGGCCTGATCTCCGTGGAAGAATTTCGTGCCATGTGGAAACTTTTTAGTTCT
CACTACAATGTTCACATTGATGATTCCCAAGTCAATAAGCTTGCCAACATAATGGACTTG
AACAAAGATGGAAGCATTGACTTTAATGAGTTTTTAAAGGCTTTCTATGTAGAGCATAGA
TATGAAGACTTGATGAAACCTGATGTCACCAACCTTGGCTAA

Clone variation with respect to NM_006240.2
1913 t=>a
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006240 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGGTTTCCTTCC
CTCCCTCCTGTGTATTCCTCATTAGAATCTATGACTGAAGAGGATCGGCTAAGAGTGGTT
CCTCGCAGCTTAAAGGGAGGCACTTTTCACACTCTGTCTTAAAATCAGAAGTTGAATTCA
TGAACACATATGATTTAGATAGAAGTCATGGGATGCAGCAGTTCTTCAACGAAAACCAGG
AGATCTGACACATCACTGAGAGCTGCGTTGATCATCCAGAACTGGTACCGAGGTTACAAA
GCTCGACTGAAGGCCAGACAACACTATGCCCTCACCATCTTCCAGTCCATCGAATATGCT
GATGAACAAGGCCAAATGCAGTTATCCACCTTCTTTTCCTTCATGTTGGAAAACTACACA
CATATACATAAGGAAGAGCTAGAATTAAGAAATCAGTCTCTTGAAAGCGAACAGGACATG
AGGGATAGATGGGATTATGTGGACTCGATAGATGTCCCAGACTCCTATAATGGTCCTCGG
CTACAATTTCCTCTCACTTGTACGGATATTGATTTACTTCTTGAGGCCTTCAAGGAACAA
CAGATACTTCATGCCCATTATGTCTTAGAGGTGCTATTTGAAACCAAGAAAGTCCTGAAG
CAAATGCCGAATTTCACTCACATACAAACTTCTCCCTCCAAAGAGGTAACCATCTGTGGT
GATTTGCATGGGAAACTGGATGATCTTTTTTTTGATCTTCTACAGAAATGGTCTCCCCTC
AGAGAGGAACCCGTATGTTTTTATGGTGACNTTGTAGATCGAGGAAAGATCCATAGAGAT
CTATGATCCTGGNGTGAGTTTCTGTCTACCCATGACCG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006240 unedited
TGGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACAGTTTAAAGATG
GAAAGACTTTATTCCATGAATAACCTGGTATTCCTCCTGTCTCCCTCAGTCACTCACAGA
TGTCAAGCTTAGTAGTTTTCTCTTGTGCTGTATGAGTTCCTTTCAGGATGTTTTCAAACC
GCTCTGGCCTCCTGGTTAGGTGGCACTCACGACAACACCACTTAAGGGTCCAACCCAGTT
TCTCACTGGCAATATGTAGGTACTAATGCTGAAACCAAATCCTATTACTGACATTTAACC
TAATCTTACCAATAACAAGCTTGCTAGGGACTTCCAAAACCCATACTATGCATCTGTTTG
TGGGATGAATTGGGATCTGCTTTTCTCTGCTACTGACTATGAATTTCAGGGGTGTTGGAA
AGGACTGTACTTGTGATTTGGGCCTAGCTGTTTCAGGGAGCCTGAGGGAAGCTCTCATTT
GTGTTTAGCCAAGGTTGTGACATCANGTTTCATCAAGTCTTCATATCTATGCTCTACATA
GAAAAGCCCTTAAACTCATTAAAGTCAATGCTTCCATCTTTGNTCAAGTCCATTTATGTN
GGCAGCTTATTGACTTGGGAATCATCAATGTGAACATTGTANTGAGAACTAAAAAAGTTC
ACATGGCACGAAATTCTTCCCACGAGATCAGGCCTGANTGATCAGTGTCAATGGCATTAA
TATGATTTCCCAGTCAGATCTGTATCTGACAGAGTTTCAACTAGAGTAGAATGACCCTNT
TGTACAAGTTTTCAATGCGGAATTTCTTGAAAGCTGGCAATGATTCAACGTTTCCATTTT
GGCCATTGTTACCAAATTCAACTGAAGGATCTCCTGGAAGTTCACCCCAAATAGTTTTCA
TGCAAAAACCCCCTGCTCCCCAAAGTTTTCTGATTTCTTGGGCTTTGAATGGAAGCCCAA
TAGGGCATTTTTCGGAAACACTCTTTTTAATATTGAAGGCCCGGTTCCTAAATCACCTCT
GGCGAAAAGCTAAACC
Restriction Sites NotI-NotI |
ACCN NM_006240
Insert Size 2700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006240.2, NP_006231.2
RefSeq Size 2873 bp
RefSeq ORF 1962 bp
Locus ID 5475
UniProt ID O14829
Cytogenetics Xp22.13
Domains EFh, IQ, Metallophos, PP2Ac
Protein Families Druggable Genome, Phosphatase
Summary This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) encodes the predominant isoform (1).
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Documents
Resources
"NM_006240" in other vectors (6)
SKU Description Size Price
RC206034 PPEF1 (Myc-DDK-tagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1 10 ug
$608.00
RC206034L1 Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1, Myc-DDK-tagged 10 ug
$908.00
RC206034L2 Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1, mGFP tagged 10 ug
$908.00
RC206034L3 Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1, Myc-DDK-tagged 10 ug
$908.00
RC206034L4 Lenti ORF clone of Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1, mGFP tagged 10 ug
$908.00
RG206034 PPEF1 (tGFP-tagged) - Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1 10 ug
$489.00 $808.00

file-alt Citations

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