BRF1 (NM_001519) Human Untagged Clone

SKU SC111637

BRF1 (untagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: BRF1
• Synonyms: BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p; TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111637 sequence for NM_001519 edited (data generated by NextGen Sequencing); ATGACGGGCCGCGTGTGCCGCGGTTGCGGCGGCACGGNNNNNNAGCTGGACGCGGCGCGC; GGGGACGCGGTGTGCACCGCCTGCGGCTCAGTGCTGGAGGACAACATCATCGTGTCCGAG; GTGCAGTTCGTGGAGAGCAGCGGCGGCGGCTCCTCGGCCGTGGGCCAGTTCGTGTCCCTG; GACGGTGCTGGCAAAACCCCGACTCTGGGTGGCGGCTTCCACGTGAATCTGGGGAAGGAG; TCGAGAGCGCAGACCCTGCAGAATGGGAGGCGCCACATCCACCACCTGGGGAACCAGCTG; CAGCTGAACCAGCACTGCCTGGACACCGCCTTCAACTTCTTCAAGATGGCCGTGAGCAGG; CACCTGACCCGCGGCCGGAAGATGGCCCACGTGATTGCTGCCTGCCTCTACCTGGTCTGC; CGTACGGAGGGCACGCCGCACATGCTCCTGGACCTCAGCGACCTGCTCCAGGTGAATGTG; TACGTGCTTGGAAAGACGTTTCTTCTCTTGGCAAGAGAGCTCTGCATCAATGCGCCGGCC; ATAGACCCGTGCCTGTATATTCCACGCTTTGCGCACCTGCTGGAATTCGGGGAGAAGAAC; CACGAGGTGTCCATGACTGCCCTGAGGCTCCTACAGAGGATGAAGCGGGACTGGATGCAC; ACAGGCCGGCGCCCCTCGGGCCTCTGCGGAGCAGCGCTCCTGGTTGCAGCCAGAATGCAT; GACTTCAGGAGGACTGTGAAGGAGGTCATCAGTGTGGTCAAAGTGTGTGAGTCCACGCTG; CGGAAGAGGCTCACGGAATTTGAAGACACCCCCACCAGTCAGTTGACCATTGATGAGTTC; ATGAAGATCGACCTGGAGGAGGAGTGCGACCCCCCCTCGTACACAGCTGGGCAGAGGAAG; CTGCGGATGAAGCAGCTTGAACAAGTCCTGTCAAAAAAACTGGAGGAGGTTGAAGGTGAA; ATATCCAGTTACCAGGATGCAATTGAGATTGAACTAGAAAACAGCCGGCCAAAGGCCAAG; GGGGGCCTGGCCAGCCTGGCAAAAGATGGCTCCACCGAGGACACCGCGTCCAGCTTGTGT; GGCGAGGAGGACACAGAGGACGAGGAGCTGGAAGCCGCGGCCAGCCACCTGAACAAAGAC; TTATACCGGGAGCTCCTTGGTGGTGCCCCCGGCAGCTCGGAAGCAGCAGGAAGCCCCGAG; TGGGGCGGCAGACCTCCGGCCCTGGGGTCCCTGCTGGACCCCCTCCCCACTGCAGCCAGC; CTGGGCATCTCAGACTCCATCCGCGAATGCATCTCCTCTCAGAGCAGCGACCCCAAAGAT; GCTTCAGGAGACGGTGAGCTGGACCTCAGTGGCATTGATGACCTGGAGATTGACAGGTAC; ATCCTGAATGAGTCGGAAGCCCGCGTGAAGGCCGAGCTGTGGATGAGGGAGAACGCCGAG; TACCTGCGGGAACAGAGGGAAAAAGAAGCAAGAATAGCGAAAGAGAAGGAGCTCGGCATC; TACAAGGAACACAAGCCCAAGAAGTCTTGCAAGCGACGGGAGCCAATTCAGGCCAGTACC; GCCAGGGAGGCCATCGAGAAGATGCTGGAGCAGAAGAAGATCTCCAGCAAGATCAATTAT; AGCGTGCTCCGGGGCCTCAGCAGCGCCGGCGGGGGCAGTCCGCACAGGGAGGATGCACAG; CCCGAGCATAGCGCCAGTGCCAGGAAGCTGTCACGAAGGAGGACGCCGGCCAGCAGAAGT; GGGGCTGACCCTGTGACCAGTGTGGGGAAAAGGTTGAGGCCTCTGGTGTCTACGCAGCCA; GCAAAGAAGGTGGCCACGGGAGAGGCTTTGCTCCCAAGCTCTCCCACCCTCGGAGCTGAG; CCTGCCAGGCCCCAGGCGGTGCTGGTGGAGAGCGGGCCCGTGTCATACCACGCCGACGAG; GAGGCTGACGAGGAGGAGCCTGACGAGGAGGACGGGGAGCCCTGCGTCAGTGCCCTGCAG; ATGATGGGCAGCAACGACTATGGCTGTGATGGCGATGAGGACGACGGCTACTGA; ; Clone variation with respect to NM_001519.3; 38 a=>n;39 c=>n;40 a=>n;41 t=>n;42 c=>n;43 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001519 unedited; TCGGAANNGNATACGACTTACTATAGGGCGGCCGCGAATTCGCACGAGGGAGAGTCTTGG; GAGGCTCCGGGCGCTGCCCGCGCGGTCGCCGGCATGACGGGCCGCGTGTGCCGCGGTTGC; GGCGGCACGGACATCGAGCTGGACGCGGCGCGCGGGGACGCGGTGTGCACCGCCTGCGGC; TCAGTGCTGGAGGACAACATCATCGTGTCCGAGGTGCAGTTCGTGGAGAGCAGCGGCGGC; GGCTCCTCGGCCGTGGGCCAGTTCGTGTCCCTGGACGGTGCTGGCAAAACCCCGACTCTG; GGTGGCGGCTTCCACGTGAATCTGGGGAAGGAGTCGAGAGCGCAGACCCTGCAGAATGGG; AGGCGCCACATCCACCACCTGGGGAACCAGCTGCAGCTGAACCAGCACTGCCTGGACACC; GCCTTCAACTTCTTCAAGATGGCCGTGAGCAGGCACCTGACCCGCGGCCGGAAGATGGCC; CACGTGATTGCTGCCTGCCTCTACCTGGTCTGCCGTACGGAGGGCACGCCGCACATGCTC; CTGGACCTCAGCGACCTGCTCCAGGTGAATGTGTACGTGCTTGGAAAGACGTTTCTTCTC; TTGGCAAGAGAGCTCTGCATCAATGCGCCGGCCATAGACCCGTGCCTGTATATTCCACGC; TTTGCGCACCTGCTGGAATTCGGGGAGAAAGAACCACGAGTGTCCATGACTGCCCTGAGG; CTNCTACAGNNAGATGAAAGCGGACTGGATGCACACAGGCCGGGCGCCCCTCGGCCTCTG; CGGAGCAGCGCTCCTGGNTGCAGCCAGAATGCATGACTTNCAGAGGACTGTGAAAGGAGT; CATCAGTGTGGTCAAAAGTGTGTGAGTCCACGCTGCNGNAGAAGCTCACGGATTTNGAGA; ACCCCCACAGTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001519 unedited; GCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTGGTAAAGAACAACAGGAACTTCCTGGTTTACATAAAACTAT; TCTTCCCCCAAACCCTTGAAAAACCCTGGCAGGAACCAAGCTGGCTACTGAGCTCTGGGC; GGGGGTAGGGGGGTCTGGCCTGCTGCGGGCCAAGTTGGGGCAAGACATACAGCCCATTTC; ATGGGGAGGATGAGGCCCCTGGGGGGCAATGAGGGACGGGTCTGGCTCAAGGGCACCCCC; TCCTAAGGACACAGGGGGAAGCCCCCTCGGCCACATTCGGGGCAGCCATGCCAGAGCTGA; GACCTCCTACGAGGGGGACCGGGGCCCTCCCCCAAAAGGCCTGGGGAGGCTCTCGGGCCT; CCGGCTGGCCTTCCTTCTCGATGGGGGGCCCGAACCCGCTTTGACCGGGAGCGCCCCTGG; CCTCTGGGTTTTGTCACGGCAACTTCCAAAGCCCGGACCTATGCCGGCTATGCCCGCACT; GCCCAACGAACTTTGCTCAGGACGGGGGGGCGCCGGCTCAAATTGTCGGCCCTAAGTCAC; CTGGCGGCAGGCCCAAGGTTTCTGGGGCCACAAACCAAGGGGGCCTGGAGCAAGGGTCCG; ACCCCCCTGCTGGTCCAGCGGCGCCAGCACGGGCACGGGCGGCTTGTCGACGCCCGCCTC; TCCCCCTTCCCACCCCCTCCGCGTCCCCCCCCCCCCCCCCATTCCGCTCCCCTTTTTTTT; TTCCTTGTTTTTCCCCCCCCCCCCCCCCCTTACGCCCCTTTTCCTTTTCCCTTCCCCCCC; TTCCCTCCTCCCCCCTCCCCCCCTTCTCCCCCCCTCCCTCCCCCCCTTCCCACTTCTCAC; CCACTCCCCTCCCCTTTCCTCCCCCCTTTATTCCCCTCCCCCCTCCACCCCCCCCTGCCC; CTCCCCACCCCCTTCCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001519
• Insert Size: 3370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001519.2, NP_001510.2
• RefSeq Size: 3660 bp
• RefSeq ORF: 2034 bp
• Locus ID: 2972
• UniProt ID: Q92994
• Cytogenetics: 14q32.33
• Domains: CYCLIN, transcript_fac2
• Protein Families: Transcription Factors
• Summary: This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).