NHP2 (NM_017838) Human Untagged Clone
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | NHP2 |
| Synonyms | DKCB2; NHP2P; NOLA2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_017838 |
| Insert Size | 720 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_017838.3, NP_060308.1 |
| RefSeq Size | 867 bp |
| RefSeq ORF | 462 bp |
| Locus ID | 55651 |
| UniProt ID | Q9NX24 |
| Cytogenetics | 5q35.3 |
| Domains | Ribosomal_L7Ae |
| Summary | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2008 Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a). |
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Resources
"NM_017838" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203033 | NHP2 (Myc-DDK-tagged)-Human NHP2 ribonucleoprotein homolog (yeast) (NHP2), transcript variant 1 | 10 ug |
$150.00
|
|
| RC203033L3 | Lenti ORF clone of Human NHP2 ribonucleoprotein homolog (yeast) (NHP2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC203033L4 | Lenti ORF clone of Human NHP2 ribonucleoprotein homolog (yeast) (NHP2), transcript variant 1, mGFP tagged | 10 ug |
$450.00
|
|
| RG203033 | NHP2 (tGFP-tagged) - Human NHP2 ribonucleoprotein homolog (yeast) (NHP2), transcript variant 1 | 10 ug |
$489.00
|
|
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